Objective To investigate the status of urogenital Ureaplasma urealyticum (Uu) infection and their drug tolerance in our area,so as to guide rational administration for the clinic.Methods Mycoplasma culture and drug susceptibility test were performed for 771 patients with urogenital infaction. The culture,identification and drug resistance of mycoplasma were carried out with one complex mycoplasma kit.Results The positive rate of Uu was 49.9%. The proportional percentage of males was 12.5% and the Uu positive rate was 22.3%,for females,the proportional percentage and Uu positive rate was 87.5% and 60.6% respectively. The population aged 21-40 was susceptible to Uu infection. The most sensitive antibioties to Uu was clindamycin (95.12%),followed by doxycycline (87.57%) and roxithromycin (86.24%).Conclusion The difference of Uu drug sensitivty was significant in different areas. So it is necessary to select antibioties by drug sensitivity. Clindamycin,doxycycline and roxithromycin may are used as preferred antibioties in treating Uu infection in our area. The drug-resistance status of spectinomycin and lincomycin was serious.

OBJECTIVE To explore the situation of bacteriological examination in every clinical department,know distribution and drug resistance of pathogenic bacteria,and offer the help about diagnosis and reasonable using antibiotics.METHODS Using the Microscan A/S-4 to identify the microbes and microbial sensitivity tests according to pathogens from more than twenties clinical departments between 2004 and 2005.RESULTS The clinical delivering rate was low,and samples were dispersive.The samples mostly were sputum,pus,urine and blood,and their rate was 52.15%.The first three pathogenic bacteria were totally 13 kinds of species in all kinds of departments.The main pathogenic bacteria in the internal medicine such as in the department of respiration,department of gerontology,department of endocrinology and so on were mostly Klebsiella pneumonia and Escherichia coli.The main pathogenic bacteria in department of neurosurgery,ICU,department of burn and department of trauma were mostly Pseudomonas aeruginosa,Acinetobacter baumannii/haemolyticus,Enterobacter cloacae and Staphylococcus aureus,These bacteria might lead to nosocomial infections in our hospital too.Their rate was 52% and drug-resistance was serious.A.baumannii/haemolyticus and E.cloacae were only sensitive to imipenem.P.aeruginosa was most sensitive to piperacillin/tazobactem and next to ceftazidime,amikacin and ciprofloxacin.CONCLUSIONS There are the serious phenomena that P.aeruginosa,A.baumannii/haemolyticus and E.cloacae are highly resistant to many antibiotics.We will much more attend and study it.The clinics must attend it and improve the delivering rates of cultured sample.

According to the theory of microscopic system reform in higher education, a total of 927 college students of different majors from Beijing University of Chinese Medicine were investigated by using SIMPP method. It indicated that through promoting the study of self-prepared, learning state and learning effect, students can enhance their learning ability of the admission acceptance. From the perspective of teachers, the students' self-study ability can be fully mobilized using individual person-alized teaching method. Then, the initiative of university education in the teaching and learning can be improved. Ultimately, the efficiency of student learning can be improved.

Interest in learning can not only stimulate students' enthusiasm for learning, but also directly affect the quality of higher education. In this paper, the questionnaire of the related factors of college students' interest was designed to carry out micro-system engineering research. The factors as-sociated with college students' interest in learning and their complex relationships were investigated by using methods of systems science and systems engineering. The results shows that the students who can actively participate in after-school learning discussions , feel good in lectures and have clear learning objectives, are interested in learning. And at the same time, the teacher's influence and his active guidance in classroom teaching will raise students' interest in learning. This study provides a more comprehensive basis and clues to improve students' interest in learning and education reform.

Objective To study the biodistribution of 131 I-2'-deoxy-1-β-D-arabinofuranosy1-5-iodouracil (FIAU) in the rat middle cerebral artery occlusion model and the expression of thymidine kinase (TK) gene in brain tissue after gene-modified stem cell transplantation,and thus evaluate the possibility of further noninvasive monitoring of stem cell transplantation therapy in cerebral infarction.Methods Adenovirus recombinant Ad5-TK-intemal ribosome entry site-brain derived heurotrophic factor-enhanced green florecent protein(IRES-BDNF-EGFP) carrying TK-IRES-BDNF gene was prepared.Cerebral infarction model was established in rats by intraluminal middle cerebral artery occlusion with nylon monofilament.Gene modified bone marrow mesenchymal stem cells were transplanted via intraparenchymal route,lateral ventricle,carotid artery and tail vein,respectively.The normal rats were used as controls.131 I- FAU was prepared to be the tracer for biodistribution study and the ％ ID/g was calculated based on measurement of the tissue radioactivity counts.The expression of TK gene was evaluated by quantitative real-time PCR (QR-PCR) and Western blot analysis.Data were analyzed with independent-samples t-test,one-way analysis of variance (ANOVA) test,and Pearson linear correlation test.Results The ％ ID/g of infarcted brain tissue in the intraparenchymal group was 0.124 ± 0.013,which was significantly higher than that in lateral ventricle group (0.052 ±0.004),carotid artery group (0.061 ±0.002),tail vein group (0.059 ±0.005) and control group (0.005 ±0.001) (t =2.913 - 5.652,all P＜0.05),while there were no statistically significant differences among the other route transplanted groups ( t =0.694 - 1.448,all P ＞ 0.05 ).The differences of ％ ID/g between the infarcted and contralateral sides of brain tissue in all transplanted groups were statistically significant (t =9.004 - 15.734,all P ＜ 0.05 ),while there was no statistically significant difference of this parameter between both sides of brain tissue in control group (t =1.511,P =0.182).The expression of TK gene in intraparenchymal group was significantly higher than other groups (t =7.482 -12.371,all P ＜0.05).The expression levels ofTK gene on QR-PCR showed a positive correlation with ％ID/g of the brain tissue ( r =0.971,P ＜ 0.001 ).Similarly,the ratio of TK/β-actin by the Western blot analysis correlated with the ％ ID/g ( r =0.899,P =0.002 ).Conclusion Intraparenchymal route may be the way of choice for cell transplantation therapy of cerebral infarction.If suitable radionuclide tracer is available,PET or SPECT may be potentially used for noninvasive monitoring of stem cell transplantation in cerebral infarction in vivo.

Abstract:Alcoholic liver disease (ALD) is one of the most common liver diseases in the world. Long-term alcoholism causes a series of pathological changes in the liver, which eventually leads to the occurrence of liver diseases with an increasing incidence. At present, significant progress has been made in the pathogenesis and pathological development of alcoholic liver disease, but the relevant mechanism of ALD has not been thoroughly studied. It is necessary to improve the existing animal model or establish a new, more comprehensive animal ALD model to simulate human ALD. Experimental animal models of ALD, especially rodents, are often used to simulate human ALD, and the ideal rodent ALD model can effectively simulate all aspects of alcohol in human liver. But so far, the commonly used animal models all have certain defects, and there is no complete animal model that can simulate human ALD. This paper reviewed the pathogenesis of ALD, related methods and influencing factors of ALD model, and provided a theoretical basis for relevant researchers to establish the ALD rodent model. 

OBJECTIVETo observe the clinical effectiveness of Sanmiao Powder (SP) combined fibrinogenase for injection in treatment of acute deep venous thrombosis (ADVT) of lower limbs.

METHODSEighty patients with ADVT were randomly assigned to two groups according to the disease course (within 7 days or 7-28 days), 40 in each group. Every time phase was also divided into two groups, i. e., one group treated with fibrinogenase for injection alone (Group A and C) and another group treated with fibrinogenase for injection + SP administration (Group B and D) , 20 in each group. The clinical effectiveness was observed after 2-week treatment.

RESULTSThe fibrinogenase for injection + SP administration showed better effects in alleviating the swelling of limbs, relieving pain, and lowering fibrinogen. Better effects were obtained in the group with the disease course less than 7 days.

CONCLUSIONBetter effect on ADVT was obtained by integrative medicine.

Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Lower Extremity ; blood supply ; Male ; Metalloendopeptidases ; therapeutic use ; Middle Aged ; Phytotherapy ; Venous Thrombosis ; drug therapy

OBJECTIVETo evaluate incidence of postoperative delirium after hip surgery in elderly patients by meta-analysis.

METHODSFrom January 1, 2014 to December 31, 2013, clinical literatures about postoperative delirium after hip surgery in elderly patients,were searched from the Pubmed. Literature extract table were formed according to inclusion and exclusion criteria. Stata-12.0 was applied for Meta-analysis. P was used to test heterogeneity of study, random-effect model was performed when I2 > 50%. Subgroup analysis was used according to stage of age, assessment scale of delirium and statistical area of literature. Begg test was used to test publication bias.

RESULTSTwenty-one literatures were included. Incidence of postoperative delirium after hip surgery in elderly patients by weighted and combination was 17% [95% CI (16%, 18%)]. Incidence of postoperative delirium after optional hip surgery was decreased more than emergency operation in included 5 literatures [OR = 0.32, 95% CI (0.22, 0.45)]. Incidence of postoperative delirium in patients less than 80 years old was 21% [95% CI (19%, 23%)], while 21% [95% CI (19%, 24%)] in patients more than 80 years old. Incidence of postoperative delirium in CAM evaluation scale was 23% [95% CI (21%, 26%)], while 19% [95% CI (17%, 21%)] in other evaluation scales. Incidence of postoperative delirium in Asian area was 17% [95% CI (15%, 20%)], while 23% [95% CI (21%, 25%)] in European and American area. There was no publication bias tested by Begg test (P < 0.05).

CONCLUSIONIncidence of postoperative delirium after hip surgery in elderly patients increases higher, especially in emergency operation. A standardizing research method is benefit for evaluate incidence of postoperative delirium after hip surgery in elderly patients, decreasing heterogeneity and publication bias.

Aged ; Delirium ; epidemiology ; Hip Fractures ; surgery ; Humans ; Incidence ; Postoperative Complications ; epidemiology ; Publication Bias

Objective To provide applied anatomic data for relevant operations of blood vessels of perisacral promontory(BVPSP). Methods The composition of BVPSP including origin, course, diameter of the middle sacral vessels, the distance between the sacral promontory and the sacral 1 transverse trunk were observed on 37 adult cadavers. Result The BVPSP is composed of the common and internal iliac vessels, the superior segment of the middle sacral vessels and the sacral 1 transverse trunk. Middle sacral artery comes from abdominal aorta. Middle sacral veins are thin walled without valves. The average diameter of middle sacral artery and vein is 1.02 mm and 2.53 mm respectively. The distance between the sacral 1 transverse trunk and the sacral promontory is 5.75 mm. Conclusion The composition of BVPSP, especially middle sacral veins, plentiful vascular anastomosis are the anatomical basis leading to massive hemorrhage in the relevant operations.

Objective To evaluate the clinical value of 18F-FDG PET-CT in detecting or diagnosing the recurrence and metastasis of colorectal cancer in the postoperative patients with rising CEA or CA19-9 during the follow-up.Methods 87 colorectal cancer patients were entered in the trial,all were postoperative,and during the follow-up were rising CEA or CA19-9 found, and then had the whole body 18F-FDG PET-CT examination.The final diagnosis of all patients were based on pathology results of re-operation,colonoscoply,a variety of imaging examination or clinical follow-up. Results For the diagnosis of recurrence or metastasis of postoperative colorectal cancer with rising CEA or CA19-9,PET-CT sensitivity was 91.0%(71/78),specificity 66.7%(6/9),accuracy 88.5%(77/87).Among the 61 patients who have recently (in 3 months) had conventional chest,abdominal and pelvic imaging examination (ultrasound,CT and MRI),32 patients PET-CT found the lesions that the conventional imaging did not found.The treatment programs of 21 patients have been changed,correction rate was 34.4%(2 1/61). Conclusion The 18F-FDG PET-CT imaging had a significant value in the diagnosis of recurrence and metastasis and restaging in the postoperative colorectal cancer with rising CEA or CA19-9 during the follow-up.