To study the mechanism of Shenkangling (SKL), a compound traditional Chinese herbal medicine, combined with prednisone in treating adriamycin-induced nephropathy in rats.

Objective To investigate etiology of acute pneumonia in children in order to provid basis for clinical diagnosis and treatment.Methods The children with acute pneumonia who were hospitalized in children's hospital affiliated to Suzhou university were selected.And the sputum of them were collected.Bacteria and virus were tested using sputum culture and direct immunofluoresence respectively.Antibodies against mycoplasma and chlamydia were detected by enzymelinked immunosorbent assay(ELISA) in paired sera. Results Microbial etiology was obtained in 360 cases (67.7%) of 532 patients.Viral infections were in 178 cases (33.5%).Bacterial infections were in 23 cases (4.3%),mycoplasma pneumoniae 50 cases (9.4%) and chlamydia pneumoniae 19 cases (3.6%),compound infections 90 cases (16.9%).Respiratory syncycial virus was the major viral pathogen,streptococcus pneumoniae were the prominent pathagens of bacterial pneumonia,followed by haemophilus influenza.Conclusions Viral infection is the most common cause of acute pneumonia in children in Suzhou area during winter and spring,followed by mycoplasma pneumoniae,bacteria and chlamydia pneumoniae.Mycoplasma pneumoniae infection is more common in children older than 3 years,chlamydia pneumoniae infection is more in infants less than 3 months,most of compound infection children were below the age of 3 years.

0.05);2)After 12,24,36 months' treatment,BP was decreased significantly in each group (P0.05).Conclusion Both combined spirono- lactone/HCTZ and captopril/HCTZ significantly reduced BP and LVMI or LVMI and the maguitude of reduction was further enhanced after prolonged treatment.

Objective To study the effect of enalapril folic acid on peripheral blood inflammatory factors in type H hypertensive of rats.Methods Sixty Wistar Spontaneous hypertension male rats were randomly divided into control group,model group,enalapril group and enalapril folic acid group,15 rats in each group.Type H hypertensive rat model were established by feeding with 3％ methionine in model group,enalapril group and enalapril folic acid group.And enalapril folic acid group were given 10 mL · kg-1 enalapril maleate folic acid solution,enalapril group were given 10 mL · kg-1 enalapril solution,and the model group were given 10 mL · kg-1 double distilled water,and 4 groups were intervened for 8 weeks everyday.The serum levels of homocysteine (Hcy),serum tumor necrosis factor alpha (TNF-α),hypersensitivity C reactive protein (hs-CRP)and soluble OX40 ligand(OX40L) were measured by enzyme-linked immunosorbent assay.The nitric oxide(NO)level were measured by End-point assay.The structure of carotid artery was observed under microscope.Results Compared with Hcy(64.22 ±6.82) μ mol · L-1 and NO(41.45 ±5.25) μmol · L-1 in the enalapril group,levels of Hcy(19.75 ±2.14) μmol · L-1 were lower,and NO(57.68 ±6.43) μmol · L-1were higher in the enalapril folic acid group with significantly (P ＜ 0.05).Compared with TNF-α (72.72 ± 6.89) pg · mL-1,hs-CRP (1.15 ±0.12) pg · mL-1,OX40L(5.24 ±0.47) ng · mL-1in the enalapril group,levels of TNF-α(58.18 ±5.76)pg · mL-1,hs-CRP(0.86-±0.11) pg · mL-1,OX40L(3.03 ±0.39) ng · mL-1 were lower in the enalapril folic acid group with significantly(P ＜0.05).Compared with the carotid artery IMT (61.14±7.73) μm,and IMCSA levels(0.12 ± 0.02) mm2 in the enalapril group,the carotid artery IMT (52.74±6.29)μm,and IMCSA (0.10 ±0.01) mm2 in the enalapril folic acid group were lower with significantly(P ＜ 0.05).Compared with the levels of carotid artery LD(951.38 ± 6.08) μm in the enalapril group,the levels of carotid LD (969.79 ± 6.17) μm in the enalapril folic acid group was higher with significantly(P ＜0.05).Conclusion enalapril folic acid can effectively reduce levels of peripheral blood Hey,TNF-oα,hs-CRP and OX40L in type H hypertensive rats,improve levels of NO,reduce inflammation,and improve the structure of carotid artery.

OBJECTIVETo observe the changes of Ag-NORs of T lymphocytes and hemorheological indexes in old patients with blood stasis (BS) syndrome.

METHODSAg-NORs of T lymphocytes and hemorheological indexes in 54 old patients with blood stasis syndrome in the BS group were determined and compared with those in the control group consisted of 36 healthy persons.

RESULTSContents of Ag-NORs of T lymphocytes in the BS group and the control group were 5.857 +/- 0.396 and 6.111 +/- 0.541, respectively, showing significant difference (P < 0.05). Some abnormal hemorheological indexes were seen in the BS group, which were significant different to those in the control group (P < 0.05 ). Correlation analysis showed that RVP, ESR, K1 and K2 were negatively correlated with Ag-NORs of T lymphocytes (r = -0.273, -0.335, -0.301, -0.276 respectively; P < 0.01 or P < 0.05), while aggregation index was positively correlated with Ag-NORs of T lymphocytes (r = 0.389, P < 0.05).

CONCLUSIONThe lowered Ag-NORs of T lymphocytes and abnormal hemorrheological changes might be one of the important reasons for the lowered immunity of aged patients with BS syndrome.

Aged ; Aged, 80 and over ; Diagnosis, Differential ; Female ; Hemorheology ; Humans ; Male ; Medicine, Chinese Traditional ; Nucleolus Organizer Region ; pathology ; Silver Staining ; Syndrome ; T-Lymphocytes ; pathology

Objective Clinical significance of using ELISA to determine ?-amyloid(A?)_(1-42) antibody levels in the sera of patients with Alzheimer's disease(AD).Methods 96 wells PVC plate was coated with A?_(1-42)peptide.Serum of AD patient was competing with mouse A?_(1-42)monoclonal antibody in this assay.The second antibody was horseradish peroxidase(HRP)conjugated goat anti-mouse IgG.Serum A?_(1-42)antibody levels were determined by ELISA.Results The sensitivity of this assay was about 1 ng/ml.The recovery rate of this test was between 96.5% and 104.7%.The residual A?_(1-42)antibody levels in human serum or horse serum after A?_(1-42)antibody was removed by absorption were less than 1 ng/ml. Serum A?_(1-42)antibody levels in 37 AD patients[(5.1?1.9)ng/ml]were remarkably lower than those in normal people[(12.6?3.3)ng/ml,P

Objective To study the role of fibulin-5 in urothelial carcinoma of bladder. Methods Fibulin-5 expression was detected in bladder cancer tissues (13 cases of G_1 and G_2, 7 cases of G_3) and normal bladder mucosa samples by Western blotting assay. Fibulin-5 cDNA was amplified by PCR and cloned into pMD-19T simple vector. The pMD-19T-Fibulin-5 vector was digested by restriction endonucleases XhoI and EcoRI to generate a XhoI-Fibulin5-EcoRI fragment that was then ligated into the identical sites in p-EGFP-Nl plasmid to synthesize p-EGFP-Fibulin-5 plasmid. The p-EGFP-Fibulin-5 plasmid was finally transfected into bladder cancer cell line 5637. The migration and invasion of untransfected, vector-transfected and fibulin-5-transfected bladder cancer cells were measured by Boyden chamber assay. Results Compared to 1. 16 ±0. 28 in the normal control, the expression of fibulin-5 protein in low grade and high grade tumors were 0. 57±0. 32 and 0. 44±0. 42(P<0. 01, respectively). However, the difference between low grade and high grade tumors was not statistically significant (P>0. 05). The successfully transfected bladder cancer cells demonstrated green fluorescent light. The migrated cell number of fibulin-5-transfected cells was 127. 6 ± 3. 1 compared with 139. 3±7. 7 for vector-transfected cells and 136. 9±5. 7 for untransfected cells (P>0. 05, respectively). In contrast, the invaded cell number of fibulin-5-transfected cells was 8. 0±3. 1 compared with 31. 5±4. 8 for vector-transfected cells and 31. 7±4. 7 for untransfected cells (P<0. 01, respectively). Conclusion Fibulin-5 is down-regulated in urothelial carcinoma of bladder and acts as a tumor suppressor gene by inhibiting the invasion of bladder cancer cells.

OBJECTIVETo investigate the chemical constituents of Dendrobium chrysotoxum.

METHODThe chemical constituents were isolated by various column chromatographic methods and structurally elucidated by spectral evidences.

RESULTTen compounds were obtained and identified as (+)-syringare sinol (1), 5alpha, 8alpha-epidioxy-24( R)-methycholesta-6, 22-dien-3beta-ol (2), trans-3-(4-hydroxy-3-methoxyphenyl)-acrylic acid octacosyl ester (3), defusin (4), 3, 4-dihydroxy benzoic acid (5), 3, 4-dimethoxy-benzoic acid (6), vanillic acid (7), 3, 4-dimethoxy-benzoic acid methyl ester (8), 3, 5-dibromo-2-aminobenzaldehyde (9), heptadecanoic acid 2, 3-dihydroxy-propyl ester (10).

CONCLUSIONCompounds 1, 2 and 6-10 were isolated from this plant for the first time.

Dendrobium ; chemistry ; Furans ; chemistry ; isolation & purification ; Lignans ; chemistry ; isolation & purification ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Vanillic Acid ; chemistry ; isolation & purification

Animals ; Combined Modality Therapy ; Endostatins ; genetics ; Genetic Therapy ; Liver Neoplasms, Experimental ; radiotherapy ; therapy ; Mice ; Mice, Nude ; Neoplasm Transplantation

Objective To provide the basis for clinical acquired immunodeficiency syndrome (AIDS) surveillance and to avoid cross infection in hospital,we study the infection status of AIDS in Shandong province.Methods The fourth-generated Akzo's ELISA kit and the fourth generated Immunoluminometric detection reagent were used for HIV antibody screening for 399 303 cases of both inpatients and outpatients from Jan.2003 to Dec.2011.Beijing WanTai ELISA kit and Se-marked rapid detection reagent were used for re-detection,and the positive samples were sent to the local CDC for confirming test by Western Blot.Results The HIV-1 antibody detection results of 129 (0.3230‰)patients were confirmed to be positive,including 54 (0.1352‰) cases of outpatients and 75 (0.1878‰)cases of inpatients.HIV infection rates in outpatients from 2003 to 2011 were 0.050‰,0.030‰,0.111‰,0.120‰,0.124‰,0.113‰,0.148‰,0.201‰,0.2152‰; and that in inpatients were 0.150‰,0.089‰,0.138‰,0.144‰,0.104‰,0.132‰,0.197‰,0.329‰,0.313‰ respectively.Among these inpatients,there were 61 cases of medical patients and 14 cases of surgical patients,and most were youths and farmers.Conclusions HIV infection rate was increasing year by year.Most inpatients whose HIV-1 antibody was positive were in the phase of AIDS.Therefore,it's very necessary to execute routine testing for inpatients and outpatients who need special examination for early diagnosis of HIV infection.