Objective To investigate the quality control and standardization of HE and immunohistochemical stainings in tissues slice.Methods In accordance with the standardization of quality control,HE and immunohistochemial stainings in tissues slice were performed,and the results were blindly compared before and after the standardization.Results The excellent rates of HE and immunohistochemical stainings in tissues slice after standardization were 92.3％ and 91.1％ respectively,and higher than those before standardization 78.9％ and 74.9％(x2 =3.841,3.801,all P ＜ 0.05); Diagnosis rate of 100 cases with liver biopsy tissue after standardization 86.0％ was significantly higher than 73.0％ before standardization(x2 =3.741,P ＜ 0.05).Conclusion The quality of HE and immunohistochemical stainings in tissues slice enhanced after standardization.

Animals ; Breast Neoplasms ; metabolism ; pathology ; Cell Adhesion ; Cell Line, Tumor ; Chemokine CXCL12 ; metabolism ; Chromosomes, Human, Pair 2 ; Humans ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Neovascularization, Pathologic ; Receptors, CXCR ; genetics ; metabolism ; physiology

The Ebola virus haemorrhagic fever outbreak began in Guinea and other West Africa countries in Feb.2014.Its high mortality cause worldwide attention.What is Ebola virus? What is its epidemiological characteristics and clinical manifestations? As pediatric medical workers,what should we do? The hot issues at present will be discussed in this article.

As an immune organ,the intestine plays the role of barrier to the bacterium and endotoxin.And the glutamine is necessary to maintain the function of intestine mucosa endothelium.It has the theoretical significance and clinical value for the growth and repair of intestine mucosa endothelium,the improvement of immune function, and the reduction of oxidant injury.The effect of glutamine on the intestine barrier and its mechanism are reviewed.

During the resistance to SARS, medical staff's medical ethics accumulation reflects generously. The test of SARS has promoted the medical staff's social responsibility consciousness, strengthened the coordination between each other and sublimed the morals in the whole society. Therefore, the sufficient realization of the accumulation of medical ethics depends on the enhancement of hygiene industry input;the display of the accumulation of medical ethics depends on the positive propaganda and encouragement to medical staff; the expansion of the medical ethics epitaxy depends on the fully excavation and utilization of the accumulation of medical ethics.

The roles of the probiotics improving the intestine flora disturbance, protecting the intestine defense and barrier functions, and modulating the microbial balance are taking more attention in present years with important theoretical significance and clinical application value. We review the effect of probiotics on the intestinal microecology and its possible mechanisms.

Objective To probe into the present deployment of human resources at TCM hospitals,for the purpose of enabling the TCM hospital administrators in their policy making and nmagement of human resources.Methods 39 hospitals in the province were subject to a descriptive stndy. Results Nursing human resources in TCM hospitals in the province were found to be in shortage in general;other characteristics include irrational structure of their academic titles,insufficient nurses with senior academic titles,yet an improving proportion of competent nurses;lack of a unified model for TCM nursing in-service education confines the growth of TCM nursing personnel and TCM nursing academic development.Cbnclusion Health authorities and hospitals should deploy nurses per nursing workload,scientifically manage the contractual nurses,optimize the makeup of nursing personnel,launch fundamental training in TCM nursing,for the purposes of higher HR efficiency and better inheritance and development of TCM nursing studies.

Objective To express human inducible eostimulator (ICOS) extracellular region and IgG Fc fusion protein, and analyze their function in allogenie lymphocyte proliferation in vitro and in vivo. Methods Human ICOS extraeellular region and IgG Fc fragment were cloned into a soluble expression vector. ICOS-Ig fusion protein was expressed and purified in CHO cells. To monitor primary MLR, Balb/c spleen T cells were isolated as responder cells, and irradiated C57BL/6 spleen cells as stimulator cells. 50 μg/ml ICOS-Ig or IgG was added to primary MLR cultures. The cells responsive rates were detected by 3 H-TdR methods. ELISA tested supernatants for eytokines (IL-2,IL-4, IL-10 and IFN-γ). T cells of each group in primary MLR were cultured as responder cells for secondary MLR, and irradiated C57BL/6 (donor) or C3H (third party) spleen cells as stimulator cells. Similar indexes were detected in secondary MLR. Then vital dye CFSE was used to study alloreactive T cell proliferation in vivo. CFSE-labeled C57BL/6 spleen cells were transferred to irradiated Balb/c mice. Mice were then intraperitoneally injected with 0. 2 mg IgG, ICOS-Ig or CsA each day.At the 3rd day after transfection, the spleen cells of the mice were harvested to detect CD4+ CFSE+ and CD8+ CFSE+ by FACS. Results In primary MLR, ICOS-Ig inhibited allogenic T-cell proliferation with inhibition rate being (58 ± 8)% in 50 μg/ml, and increased IFN-γ secretion. In secondary MLR, ICOS-Ig specifically inhibited the proliferation of donor spleen cells with inhibition rate being (42±8)%, and in ICOS-Ig group the levels of IL-4 and IL-10 were lower and the level of IFN-γ higher than in IgG group. However, ICOS-Ig didn't inhibit the proliferation of third-party spleen cells. In the CFSE dye assay, CFSE intensity of CD4+ and CD8+ T cells in ICOS-Ig and CsA groups was stronger than that in control group (P < 0. 05), while CFSE intensity in combined treatment group were even stronger than that in ICOS-Ig and CsA groups (P<0. 05). Conclusion ICOS-Ig could inhibit allo-reactive T cell proliferation in vitro and in vivo, and induce donor-specific T cell hyporesponsiveness specifically.

Objective To research the effect of the baicalin to the expression of interferon(IFN)-α and IFN-β in the BALB/c mouse infected by respiratory syncytial virus(RSV),and discussed the antiviral of mechanism.Methods To establish the BALB/c mouse model of RSV,the effects of IFN-α and IFN-β in the BALB/c mouse infected by RSV were detected by ELISA.Results The IFN-α and IFN-β in the BALB/c mouse infected by RSV could be evaluated significantly by Baicalin.Conclusion The Baicalin regulates the level of IFN in the BALB/c mouse infected by RSV.

Objective To investigate the effect of alteration of CRELD1 gene expression on the related genes in the endocardial cushion development.Methods Over-expression and silence of CRELD1 gene were realized by the construction of lentiviral vector.Afterwards,the lentiviral vectors were used to infect the human fetal lung fibroblasts (HFL)-I.All of the cells were divided into the following 5 groups:the blank control group,the negative control group of interference,the interference group,the negative control group of over-expression,and the over-expression group.Western blot and real-time fluorescent quantitative polymerase chain reaction were applied to examine the mRNA and protein expression of CRELD1,Sox9,Aggrecan,Scleraxis and Tenascin-C.Results The DNA sequences of 2 recombinant plasmids pLV3-shRNA-CRELD1 and pLV4-CRELD1 matched very well with those which were designed according to the DNA sequence analysis.HFL-I was successfully infected with lentiviral vectors and displayed fluorescent green light under inverted fluorescence microscope.The results of real-time PCR detection and Western blot test were consistent:expressions of Sox9 and Aggrecan in the interference group were significantly higher than those in the negative control group of interference,while the expressions of the 2 genes in the over-expression group were significantly lower than those in the negative control group of over-expression.Expressions of Scleraxis increased in both the interference group and the over-expression group when compared with the negative control groups respectively.Compared to the corresponding negative control groups,Tenascin-C expression decreased markedly in the interference group,whereas it increased significantly in over-expression group.Conclusions CRELD1 gene has negative effect on the expression of the related genes Sox9 and Aggrecan in the endocardial cushion development,whereas it has positive effect on the Tenascin-C expression.It serves as a theoretical framework to illustrate the effect of CRELD1 gene on the atrioventricular septal defect.