Objective To monitor Staphylococcus infection in a hospital,and trace the source of Staphylococcus isolated from infectious wound-related hospital environment by molecular analysis. Methods By combination of fluorescence quanti-tative polymerase chain reaction (FQ-PCR)and culture method,environmental specimens related to 5 patients with wound infection were taken and performed multilocus sequence and MecA gene typing analysis. Results A total of 71 environmen-tal specimens were taken,Staphylococcus accounted for 36.62% (n= 26),88.46% (23/26 )of which were MecA+ methi-cillin-resistant strains. 77.78% (7/9)of Staphylococcus aureus (S. aureus)and 95.00% (19/20)of coagulase negative Staphylococcus (CNS)were methicillin-resistant strains. Multilocus sequence analysis revealed that ST239 (n= 6)was the most common sequence type in S. aureus;Staphylococcal cassette chromosome mec (SCCmec)analysis showed that the ma-jor type of S. aureus was typeⅢ,and CNS were typeⅢandⅣ. Conclusion Staphylococcus is common in healthcare-associated infection,and most Staphylococcus are multidrug-resistant,continuous monitor on drug-resistant Staph-ylococcus is necessary,and risk of Staphylococcus variant to medical institutes need to be paid attention.

To better understand the current status of medical students in clinical medical ethics,we design the relevant questionnaire,with 468 medical students conducting a questionnaire survey.Findings in general are more satisfactory.but there are great differences on certain issues.Educational Strategies:medical education curriculum should open more humanities courses and post-clinical teachers should pay close attention to ethics education for medical students.

Objective:To explore the effect of the labor abdominal pressure training device on the second stage of labor and the outcome of labor in primiparas undergoing epidural analgesia.Methods:The healthy primiparas who had patient controlled epidural analgesia were randomly divided into the observation group (126 cases) and the control group (122 cases). The control group used conventional oral instruction to force after entering the second stage of labor. The women in the observation group used the labor abdominal pressure training device to guide the force. The time required to learn to exert force, the second stage of labor and the outcome of labor were observed.Results:The parturient women learned the correct time to exert force, the time of the second stage of labor, the self-efficacy score of the second stage of labor, and the post-natal fatigue score were (13.56 ± 5.32) min, (76.32 ± 10.58) min, (121.38 ± 17.52)points, (8.56 ± 0.72) points in the observation group, and (25.47 ± 7.65) min, (105.21 ± 18.41) min, (101.42 ± 14.32) points, (13.31 ± 0.46) points in the control group, the differences between the two groups were statistically significant ( t values were -10.326-11.002, all P<0.05). The maternal spontaneous delivery rate, fetal assisted delivery rate, forceps assisted delivery rate, perineal lateral cut rate, perineal median cut rate, perineal tear>Ⅰ°rate were 94.44% (119/126), 4.76% (6/126), 0.79% (1/126), 9.52% (12/126), 11.11% (14/126), 6.35% (8/126) in the observation group, and 85.25% (104/122), 12.30% (15/122), 2.46% (3/122), 29.51% (36/122), 4.10% (5/122), 9.84% (12/122) in the control group,the differences between the two groups were statistically significant ( χ2 values were 4.582-9.437, P<0.05 or 0.01). Conclusions:The use of an abdominal pressure training device for labor in patient controlled epidural analgesia for pregnant women can shorten the second stage of labor and study the time of exertion, improve the self-efficacy of the second stage of labor, reduce the degree of perineal injury and fatigue, reduce the vaginal birth rate, and help protect Mother and child are safe.

Objective To observe effect of astragalus injection on levels of blood selenium,T cell immunity function and cytokines with viral myocarditis in children. Methods Fourty two patients with viral myocarditis in children, receiving the therapy of Astragalus injection Observing the levels of blood selenium and cytokine, including interleukin-1 (IL-1), IL-6, tumor necrosis factor-alpha (TNF-?),and including estimation of T lymphocyte subsets and cardiac function too. Results The result showed that in post-treatment, the levels of blood selenium were significantly higher (P

Objective To investigate the expression and the effect of nuclear factor-?B(NF-?B) in neonatal sepsis.Methods We separated 77 newborn infants into 3 groups, which were septic group (26 cases),non-septic group (31 cases) and control group (20 cases). NF-?B existed in PBMC was detected in 3 different periods, including at admission, after the 24th hour and 48th hour of admission, of the septic group and the non-septic group by flow cytometry. At the same time, the sample of the septic group and the non-septic group were drawn for blood cultures at admission before using antibiotics.Results The expression of NF-?B in septic group was more significant than that in the other 2 groups (P

Objective To explore the role of Tanggankang Capsule in decreasing blood sugar, regulating blood lipid and protecting liver for the patient of type 2 diabetes with hepatic injury. Methods 60 cases of type 2 diabetes with hepatic injury, and with TCM syndrome of stagnation of liver-qi and deficiency of spleen, dampness-heat and blood stasis were enrolled in the study. Patients were randomly divided into treatment group (Tanggankang Capsule group, n=30) and control group (Metfofmin and Glucurolactone group, n=30), and were administrated for 8 weeks. Results The total effective rate for the improvement of TCM syndromes was 89.9% in the treatment group and 62.4% in the control group, with significant difference between the two groups (P

Objective To find a new algorithm for PET/CT image fusion.Methods A variational model was used based on the wavelet transform.Firstly,PET and CT images were decomposed using wavelet transform.Then,images in approximate channel and detail channel were fused according to the two proposed assumption.Finally,decomposed images were synthesized to form fused image.Results Compared with the results form MATLAB wavelet fusion toolbox,the experimental results showed that the new variational image fusion model could provide more accurate result for target location in radiotherapy planning.Conclusion According to experiments,the new algorithm can reach good results and meet requirement of clinical demands.

Objective To explore the expression of galectin-3 in human gastric carcinoma tissues and to investigate its clinincal significances.Methods Immunohistochemistry method was used to detect the expression of galectin-3 in 52 pairs of specimens of gastric carcinoma tissues,33 atypical hypcrplasia of gastric tissues and 20 gastritis tissues.Expression levels of galectin-3 in different tissues were compared and analyzed.The relationship between expression levels and clinical parameters was investigated.Results The positive rates of galectin-3 were 94.2 ％ (49/52),80.0 ％ (16/20) and 33.3 ％ (11/30).The positive rates of galectin-3 were higher in gastric carcinoma than those in atypical hyperplasia and gastritis (Z =2.181,6.160,3.611,all P ＜ 0.05).The expression levels of galectin-3 in lymph node metastasis gastric carcinoma,poorly differentiated gastric carcinoma were 100 ％ (22/22),100 ％ (45/45) respectively.Galectin-3 expression in patients without lymph node metastasis,high differentiation in gastric carcinoma were 78.6 ％ (27/30),57.1％ (4/7)respectively.The expression levels of galectin-3 were correlated with metastasis and differentiation,with higher expressions in the cases with metastatic lymph nodes (Z =12.463,P ＜ 0.05) and in poor differentiation tissues (Z =3.245,P ＜ 0.05).Conclusion Galectin-3 is overexpressed in gastric carcinoma tissues,indicating that both of them may play roles in the pathogenesis of gastric carcinoma.The overexpression of galectin-3 is correlated with metastatic lymph nodes and poor differentiation,indicating that galectin-3 may be prognostic indicator of gastric carcinoma.

ObjectiveTo investigate whether advanced glycation end products (AGE) induce monocyte chemoattractant protein-1 (MCP-1) expression in mouse podocytes in vitro and the role of AGE receptor (RAGE). MethodsThe effects of AGE, CML, S100 protein and neutralizing antibody against RAGE on MCP-1 gene and protein expressions were examined by RT-PCR and ELISA in mouse podocytes. ResultsRAGE was detected in undifferentiated and differentiated podocytes. AGE and CML dose-dependently stimulated MCP-1 expression in podocytes. At 8 h of incubation, AGE-and CML-treated podocytes produced more MCP-1 than that treated with bovine serum albumin (BSA), the MCP-1 concentrations being (7.44?1.01 and 8.06?0.96)ng/L for AGE and CML respectively vs (3.77?0.39)ng/L for BSA (both P

Objective To investigate the epidemiological characteristics of children infected by mycoplasma pneumoniae(MP)over the last five years in Kunming region.Methods Indirect immunofluorescence assay was used to detect blood MP-IgM of hospitalized patients from January 2003 to December 2007 in order to determine the age and gender distribution characteristics of MP infection and to investigate the epidemiological features of the five years' results.Results Infection incidences by MP in Kunming region over the last five years were 20.9%,14.3%,17.5%,15.7%and 19.5%,respectively.Statistical significance was found among the groups mentioned above(P＜0.01).The MP infection incidences in different age groups were 10.7%(～1 year old),20.5%(～3 years' old),21.5%(～6 years' old)and 21.7%(～14 years' old).MP infection incidences showed age and gender characteristics(P＜0.01).Infants showed lower NIP infection incidence and infection incidence was higher in female.The epidemiological investigation indicated that MP infection incidences varied from year to year(P＜0.01).The infection incidences of 2003 and 2007 by MP were higher than those of the other years.There were epidemiological differences in infection incidences by NIP over last five years(summer,autumn and winter).The MP infection incidences showed seasonal differences(P＜0.01).Conclusion Mycoplasma pneumoniae was the main local causative agent responsible for respiratory tract infection in children aged under one years' old in Kunming region.Over the last five years,there were two outbreaks in the local area,but with no seasonal regularity of epidemiology.