For healthy term or preterm neonates who do not require resuscitation after birth, delayed cord clamping (DCC) for 1-3 min has been shown to have a beneficial effect on neonatal and infant health. DCC is recommended as a standard procedure by the World Health Organization. Umbilical cord milking (UCM) can be considered as an alternative when DCC is unfeasible. However, the safety of UCM in preterm infants less than 28 weeks still needs more evidence.

ObjectiveTo explore the influence of locus of control,self-efficacy and self-esteem on shame.MethodsTotal 924 students from six primary and middle schools in Chengdu were recruited by stratified sampling.They were assessed with locus of control,self-efficacy,self-esteem,and shame questionnaire.Results ( 1 ) Shame was significantly negatively related with self-efficacy (r =- 0.248 ) and self-esteem (r =- 0.318 ),and was significantly positively related with locus of control (r =0.185 ).Locus of control was negatively associated with self-efficacy(r =- 0.214) and self-esteem (r =- 0.254).Self-efficacy was positively related with self-esteem (r =0.440) ; (2) Self-esteem played partial mediating role between shame and locus of control as well as self-efficacy; ( 3 ) Self-efficacy played partial mediating role between locus of control and self-esteem as well as shame ; (4) High self-esteem group and low self-esteem group existed significant differences in whole structure equation model.Based on the whole model,the high self-esteem group reduced the path from locus of control to self-esteem ; and low self-esteem group reduced the path from locus of control to self-efficacy.ConclusionLocus of control and self-efficacy are not only directly but also indirectly associated with shame by self-esteem,and self-efficacy would be regulated by the level of self-esteem.

Objective To explore the influence and potential protective effects of non-specific panMetrix Metalloproteinases(MMPs) inhibitor Doxycycline on hyperoxic lung injury and lung development in neonatal rats.Methods Neonatal SD rats were divided into 4 groups randomly within 12 hours after birth:air with normal saline(AN),air with doxycycline(AD),hyperoxia with normal saline(ON),and hyperoxia with doxycycline group(OD).Hyperoxia groups(ON and OD) were exposed to ≥90% O2.Doxycycline 20 mg/kg or equal volume of 0.9% saline(Doxycycline concentration 2 mg/ml) was administered by gastric gavage,twice daily from day 1 to experimental day,but it didn't exceed 14 days.Stereological study was carried out at day 1,3,7,14 and 21 after birth.Results Enlarged mean alveolar area was noted both in hyperoxia and Doxycycline groups.OD group had larger star volume than ON group on day 14.Except ON group,all other groups had increased alveolar septum and the condition was worsened by hyperoxia with Doxycycline.The percentage of collagen in lung parenchymal tissue in ON group increased persistently.This increasing trend was stopped in OD group on day 14,and collagen percentage had no significantly difference between OD and AN groups on day 14 and 21.Conclusion Both hyperoxia and Doxycycline influence neonatal lung development,percentage of coUgen in lung parenchymai can be reduced by Doxycycline in hyperoxic lung injury.

Objective To establish a rapid and sensitive chemiluminescence immunoassay for detecting squamous cell carcinoma antigen (SCCA)in human serum.Methods Fluorescein isothiocyanate (FITC)and N-(aminobutyl)-N-(ethylisoluminol)(ABEI) were used to label two different monoclonal antibodies of anti-SCCA.SCCA in serum combined with labeled antibodies and formed a sandwiched immunoreaction.After adding the substrate solution,the relative light unit of ABEI was measured.Magnetic particles coated with anti-FITC antibody were used as solid separation carrier.Results The results demonstrated that the method was linear to 22 ng/mL with a detection limit of 0.025 ng/mL.The coefficient of variation (CV)was less than 6% and 7% for intra-assay and inter-assay precision,respectively.Compared with the commercial kit,the proposed method showed a correlation of 0.990 1.Conclu-sion chemiluminescence immunoassay based on magnetic particles displays acceptable performance for quantification of SCCA and is appropriate for use in clinical diagnosis.

Tumor inhibiting protein p33ING1b is the key expressive product of inhibitor of growth 1.It plays critical role in cell multiplication ,period control ,senescence ,repair of DNA damage ,apoptosis ,and chroma-tin remodeling.The abnormal expression of p33ING1b is closely related to the occurrence and development of tumor.This paper reviews tumor inhibiting protein p 33ING1b in the research development of tumors in digestive system.

Objective To evaluate the efficacy of seretide (fluticasone/salmeterol) in treatment of patients with cough variant asthma(CVA). Methods 86 CVA patients were treated with fluticasone/salmeterol propionate 8 weeks, after 8 weeks, 86 patients were randomly assigned to treatment group and control group, each group with 43 patients, the treatment group continued medicine for 4 weeks, the control group without any treatment.The two groups of patients FENO measurement values, cough symptom score before treatment and after treatment for 8 weeks, 12 weeks were compared. Results After treatment for 8 weeks, cough symptom scores and FeNO in the two groups values decreased significantly compared with those before treatment(P<0.05), 12 weeks after treatment, treatment group cough product, FeNO values, were lower than in control group(P<0.05).Conclusion Fluticasone salmeterol powder inhalation can effectively improve the symptoms of cough cough variant asthma.Symptom score and FENO value can be used as a monitoring index to evaluate the therapeutic effect of CVA, and the FENO value is more effective, which has the advantages of non-invasive, simple, safe, rapid and repeatable.

Objective To investigate the predictive value of C-reactive protein(CRP)and serum calcium in the patients with acute pancreatitis(AP).Methods 85 cases of AP in our hospital from January to December 2013 were selected as the study subjects,in-cluding 40 cases of mild acute pancreatitis(MAP)and 45 cases of severe acute pancreatitis(SAP).40 cases of normal volunteers were selected as the control group.The levels of serum calcium and CRP were detected in the three groups by the automatic bio-chemical analyzer and turbidimetric immunoassay.Results The levels of serum calcium in the MAP and SAP groups was on the low side and significantly lower than that in the control group,but which in the MAP group were higher than that in the SAP group,the differences were statistically significant(P <0.05).The level of serum CRP in the MAP group and SAP group was high-er than that in the control group,and which in the SAP group was higher than that in the MAP group,the difference was statistical-ly significant(P <0.05).The level of serum calcium on the third after admission in the MAP and SAP groups was significantlyin-creased compared with the day of admission(P <0.05),but the levels of CRP in the MAP and SAP groups were significantly de-creased,the difference was statistically significant(P <0.05).The CRP detection was more in clinic,but the experiment indicating that simultaneous detection of serum CRP and calcium could significantly increase the accuracy,sensitivity and specificity of clinical judgement,the difference had statistical significance(P <0.05).Conclusion Serum calcium and CRP can be used as the predictive indexes for the condition progression and prognosis of acute pancreatitis and their joint examination can improve the sensitivity,spe-cificity and accuracy of the detection.

Objective To explore the primary treatment of type 2 diabetes, clinical effect of rosiglitazone combined with metformin treatment.Methods In 40 patients with type 2 diabetes mellitus patients implementation of routine treatment for metformin, and 40 patients in the control group based on the use of rosiglitazone intervention treatment, two groups of patients in our hospital from January 2015 to December 2016 were compared between the two groups.Results Treatment efficiency, obvious effective rate of observation group were higher in the observation group was 95%, while only 82.5% of the control group,comparison between groups showed significant differences(P<0.05);the two groups of patients with fasting blood glucose levels, 2 hour postprandial blood glucose level There were no significant difference before treatment, after treatment group observation group significantly improved, comparison between groups showed significant differences(P<0.05).Conclusion Rosiglitazone Combined with metformin in newly diagnosed type 2 diabetes clinical observation, which can help to improve the clinical symptoms of patients, reduce blood glucose, compared with pure medication.To improve the treatment effect, it is worthy of reference.

Objective To explore the etiology of patients with chief complaint of vertigo in the department of neurology in order to reduce thc rate of misdiagnosis.MethodsA total of 367 patients with chief complaint of vertigo in our department of neurology were followed up.The associated medical history,symptoms and physical examination were obtained.ResultsThe main diagnoses for the 367 patients were benign paroxysmal positional vertigo (BPPV) ( 219,59.7％ ),posterior circulation ischemia (PCI) ( 65,17.7％ ),migraine ( 31,8.4％ ),hypertension ( 18,4.9％ ) and psychogcnic vertigo ( 17,4.6％ ).Conclusions Presentation of vertigo can be clinically diagnosed in most discases,with the most common causc of BPPV.Combination of the prominent clinical features,physical examinations and especially Dix- Hallpike maneuver may guide the general physicians to a most proper cause of vertigo.

Objective To examine the differences in the level of sedation and adverse effects produced by equivalent analgesic doses of remifentanil,sufentanil and fentanyl.Methods Eighty ASA Ⅰ female patients aged 18-39 yr with BMI of 18-25 kg/m2 scheduled for laparoscopic surgery under general anesthesia were randomly divided into 4 groups ( n =20 each):group control (group C) ; group remifentanil ( group R) ; group sufentanil (group S) and group fentanyl (group F).Remifentanil 2 μg/kg,sufentanil 0.2 μg/kg and fentanyl 2 μg/kg in normal saline 10 ml were infused iv over 2 min in groups R,S and F respectively.Depth of sedation was assessed and scored using OAA/S scale (5 =alert,1 =no response to prodding) and wavele index (WLI),before (baseline) and at 2,4,6,8 and 10 min after drug administration.Besides RR,pulse oxygen saturation,BP,HR were also monitored.The incidences of apnea,muscle rigidity,nausea and vomiting,pruritus,vertigo,bradycardia,profuse sweating and skin rash were measured and calculated.Results The 4 groups were comparable with respect to age,BMI and height.The lowest values of OAA/S scores,WLI and RR were significantly lower in groups R,S and F than in group C.Sufentanil produced the deepest sedation among the 3 opioids.Remifentanil produced strongest respiratory depression and nausea and vomiting.Conclusion The equivalent analgesic dose of sufentanil produces deeper sedation than that of remifentanil and fentanyl while remifentanil has the greatest impact on RR.