1.Influence of metformin on the protein expression of IRS-1 in target tissues or OLETF rats
Dingqiong PENG ; Yan GAO ; Yu CHEN
Chinese Journal of Diabetes 2000;0(06):-
Objective To investigate the molecular mechanism of metformin. Methods The changes of protein expression of IRS-1 in liver, skeletal muscle and adipose tissues after treatment with metformin for OLETF rats, a model of spontaneous type 2 diabetes mellitus, were measured by Western blot analysis, and compared with those before treatment. Results 22 weeks after the treatment with metformin, the protein expression of IRS-1 was significantly increased in liver (P0. 05) ;and the protein expression of IRS-1 in adipose tissue was significantly decreased (P
2.Effects of antisense-CXCR4 on the VEGF-C mRNA expression in colon cancer cell
Yiliang PENG ; Houjie LIANG ; Yu CHEN
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To investigate the effects of transfection of chemokine receptor CXCR4 antisense-RNA on the functional expression of VEGF-C mRNA and the invasive ability of colon cell line HT-29 in vitro.Methods PCR primers were designed according to the coding sequence of CXCR4 gene.EcoR Ⅰ and BamH Ⅰ recognition sequences and cutting sites were added to the 5' end of the antisense primer.The purified PCR fragment was retro-inserted into the cloning site of eukaryotic expression vector pcDNA3.1(+).The pcDNA3.1(+)for CXCR4 antisense-RNA was transfected into colon cancer HT-29 cells by liposome transfection.The expression of VEGF-C mRNA was detected by RT-PCR.The expression of CXCR4 protein was determined by Western blot.At the same time,cell growth kinetics was assessed by MTT assay,and in vitro invasive ability was assessed with Boyden chamber.Results CXCR4 antisense-RNA recombinant was successfully constructed.The expression of VEGF-C mRNA in antisense-CXCR4 transfected(HT-29tran)group decreased by 54.2%,while the expression of VEGF-C mRNA in vacant-transfected(HT-29KZ)group only decreased by 9.4% campared with non-transfected(HT-29)group.The difference between the two groups mentioned above was remarkable(P
3.The Influence of Unilateral and Bilateral Amplification on the Effect of Hearing Aid Evaluation
Yu CHEN ; Yue WANG ; Wei WANG ; Taisheng CHEN ; Peng LIN
Journal of Audiology and Speech Pathology 2014;(2):184-186
Objective To study the influence of unilateral and bilateral amplification on the effect of hearing aid evaluation .Methods Using the subjective method that International Outcome Inventory for Hearing Aids (IOI-HA) and objective method that medium acoustic intensity (65 dB SPL) word recognition score(WRS) to evaluate the effect of unilateral and bilateral hearing aid fitting of middle -aged severe sensorneural hearing loss .Results Hearing aid were used for severe sensorneural hearing loss and the improvement of monosyllables and sentences in quiet and noise test of unilateral were 35 .73% ,43 .15% ,43 .23% ;the improvement of monosyllables and sentences in quiet and noise test of bilateral were 37 .90% ,51 .33% ,54 .86% .The WRS of bilateral was higher than unilater-al .The score of IOI-HA was 15~37 ,meaning patients with severe sensorneural hearing loss were satisfied with hearing aid ,and there was no statistical significance between unilateral and bilateral fitting .Conclusion The bilat-eral hearing aid fitting was better than unilateral .Binaural hearing loss are recommended to fit bilaleral hearing aids .
4.Expression of cytokines Th1 and Th2 in patients with esophageal squamous cell carcinoma.
Peng-Cheng CHEN ; Jian-Guo FENG ; Yu-Tian LING
Chinese Journal of Oncology 2007;29(11):850-851
Adult
;
Aged
;
Aged, 80 and over
;
Carcinoma, Squamous Cell
;
immunology
;
pathology
;
Cytokines
;
blood
;
Esophageal Neoplasms
;
immunology
;
pathology
;
Female
;
Humans
;
Interferon-gamma
;
blood
;
Interleukin-10
;
blood
;
Interleukin-12
;
blood
;
Interleukin-4
;
blood
;
Interleukin-5
;
blood
;
Lymphatic Metastasis
;
Male
;
Middle Aged
;
Th1 Cells
;
immunology
;
Th2 Cells
;
immunology
;
Tumor Necrosis Factor-alpha
;
blood
5.Recurrent fungus infections tricuspid endocarditis: a case report.
Hai-qiang HU ; Si-yu CAI ; Peng CHEN
Chinese Journal of Cardiology 2007;35(1):77-77
Adult
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Endocarditis
;
microbiology
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Humans
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Male
;
Mycoses
;
Recurrence
6.Expression of Nogo-A mRNA and Nogo-A protein in brain tissue of neonatal mice with ischemic-hypoxic brain damage.
Hua WANG ; Yu-jia YAO ; Da-peng CHEN
Chinese Journal of Pediatrics 2006;44(10):792-793
Animals
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Animals, Newborn
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Blotting, Western
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Brain
;
metabolism
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Disease Models, Animal
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Hypoxia, Brain
;
metabolism
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Immunohistochemistry
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Myelin Proteins
;
genetics
;
immunology
;
metabolism
;
Nogo Proteins
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RNA, Messenger
;
metabolism
;
Rats
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
;
Time Factors
7.Characteristic of Liver Injury Induced by Simvastatin Combined with HRZ in SD Rats
Shenghao LI ; Jie CHEN ; Yingrong DU ; Mingli YU ; Jiangli PENG
Journal of Kunming Medical University 2016;37(7):1-5
Objective To study the characteristics of liver injury induced by simvastatin combined with HRZ (Isoniazid,Rifampicin and Pyrazinamide) in SD rats.Methods Fifty-four 8-week-old SD rats were randomly divided into 3 groups:group A (control),group B (HRZ) and group C (simvastatin combined to HRZ),half of each group were male.We calculated the accurate dose respectively before those rats were given intragastrical administration of corresponding drugs.Six rats were killed in each group on 10th,20th and 40th day,respectively.Before this,blood was fastened from femoral of every rat that would be killed to test liver function,liver tissue slices were made in order to observe the pathological characteristic.Results Alanine amiotransferase of group C elevated in line with time and reached statistic difference on 40th day,furthermore,it was significantly higher than group A (P<0.05).Total bilirubin and direct Bilirubin of group C were significantly higher than those of group A from the beginning to the end (P<0.05),however,they declined rapidly on 10th day,this trend also had statistic difference (P<0.05) At the end of this experiment,hepatic cords was disordered slightly,but swelling liver cells and vacuolar degeneration were observed,the nuleus of cell condensed.Soakage of monocytes,neutrophils,and lymphocytes occurred in the portal and lobule regions,or even spotty necrosis occasionally.Conclusion Cholestasis occurs at the early stage when simvastatin is combined with HRZ in SD rats,however,it has a rapidly degressive trend.In contrast,Alanine amiotransferase elevates,furthermore,pathological injury or even spotty necrosis can emerge in liver tissue slices.
9.A Qualitative Research of Medical Humane Care in Improving Doctor-patient Relationship
Peng HAN ; Xiaoyun CHEN ; Tieshan ZHANG ; Zhongguang YU ; Shuqiang XU
Chinese Medical Ethics 2014;(2):284-287
Using the method of semi -structure interviews to pre-interview 6 experts and officially interview10 experts , then the record of the interview was compiled into the paper .Using the grounded theory method to encode material of the interview , a summary was made for each case base on data collation and analysis .This paper ana-lyzed the humanities concern the connotation of the application in the construction of a harmonious doctor -patient relationship and the specific measures .On the basis of the application of humanistic care in the construction of a harmonious doctor -patient relationship , significance and limitation is discussed .
10.Simultaneous Determination of Naringin, Hesperidin and Atractylodin in Yun-Pi Powder Extract by HPLC
Daye CHEN ; Wen LIU ; Xiaowei SHI ; Yu PENG
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(5):1020-1024
This study was aimed to simultaneously determinate the content of naringin, hesperidin and atractylodin in Y un-Pi (YP) Powder extract by HPLC. A Thermo ODS-2 column (250 mm í 4.6 mm, 5 μm) was used for the gra-dient elution with a methanol-water of 0~5 min (30%~40% A), 5~15 min (40%~50% A), 15~20 min (50%~79%A), 20~30 min (79%~100% A), 30~45 min (100% A), 45~55 min (100%~30% A), 55~60 min (30% A). The col-umn temperature was at 30℃. The detection wavelength was at 283 nm. The flow rate was 1 mL·min-1. The linear range was determined to be 0.009 16~0.137 00 μg (r = 0.999 9), 0.006 22~0.091 30 μg (r = 0.999 8), 0.003 58~0.053 70 μg (r = 0.999 9), respectively for naringin, hesperidin and atractylodin. The average recovery rate of pterodontic acid was 103.51%, 96.82% and 97.65%. And RSD was 1.37%, 1.41%, 1.49% (n = 6). It was concluded that the method had good specificity and repeatability. It was considered as a good tool for the content determination of naringin, hesperidin and atractylodin in YP Powder extract.