Ten cases infected with Taenia saginata were treated with Bithionol, 2, 2' thiobis (4,6-dichlorophenol) which is well known as an excellent anthelminthhic against paragonimiasis and their follow-up studies were carried on within 3 to 12 months after treatment. Bithionol, 50 mg dose per kg of body weight was divided into two equal dose and administered orally in 30 minutes intervals. Three hours later, this was followed with a sodium sulfate or magnesium sulfate purge. The strobilas without scolices were found in the stools after the medication and the follow-up studies have shown the complete deworming in all cases. It is well tolerated, toxic effects being uncommon and when present, limited to mild gastro-intestinal disturbances.
parasitology ; helminth ; cestoda ; Taenia saginata ; chemotherapy ; bithionol

The phamacological and anthelmintic action of Lysimachia clethroides were examined. Upon examining the action of water, ether, alcohol and acetone extrcts of the root on smooth muscle, it was found that the active principle was weakened by heating. Water and ether extracts inhibited the dehydrogenase of the worm. In 50 percent of 8 areas studied, Taenia were completely eliminated with 2.5-4.0 gm doses of the ether extracts, and no toxic effect was observed by the administration of the above-mentioned doses.
parasitology-chemotherapy ; Taenia sp. ; Lysimachia clethroides

A total of 60 patients with Hymenolepis nana infection were treated with a single oral dose of praziquantel in two different dose levels. Twenty-nine cases treated with praziquantel in a single dose of 15 mg/kg body weight produced a 100% cure rate. On the otehr hand, 30 out of 31 patients who received a single dose of 25 mg/kg body weight cured completely, but only one case had counts of 200 EPG of feces in each tests on the 20th and 21st days after treatment. The cure rate was 96.8% and the mean egg reduction rate of 99.8 per cent was obtained. Side effects were mild and transitory. In a few cases, abdominal pain, vertigo, headache and diarrhea were complained in a few hours after medication. Clinial hematology, serum biochemistry and urinalysis were performed immediately before and the next day after treatment in all cases. There were no significant abnormalities detected in these tests.
parasitology-helminth-cestoda ; Hymenolepis nana ; chemotherapy-praziquantel

The present study was undertaken to observe the morphological changes of Clonorchis sinensis obtained from experimentally infected rats treated with praziquantel (Biltricide) which is a new anthelmintic agent with high activity against a broad spectrum of cestode and trematode species. For this study, the rats were infected experimentally with about 50 metacercariae of C. sinensis and were given praziquantel a single dose of 600 mg per kg of body weight at 5 weeks after infection. The Clonorchis worms were isolated from the bile ducts of the rats which were autopsied on the 4th day after treatment. After isolation the parasites were observed their shape and motility in the medium of 37 C physiological saline solution, and then the fine structure of the tegument of C. sinensis was studied by means of light, scanning and transmission electron microscope. The findings of the observation were compared with those of untreated parasites. The results are as follows: All the isolated worms moved actively in the medium of physiological saline solution (37 C). A majority of the parasites obtained from the treated rats showed a large balloon shaped structure on the surface between oral and ventral suckers. But such structure has never seen in the parasites obtained from control rats. By the scanning electron microscopic observation, the regular pattern of the tegumental ridges was significantly changed on the outer surfaces of parasites obtained from the treated rats as compared with those of the control rats. By the transmission electron microscopic observation, the numerous mitochondriae in the syncytial tegumental layer of the treated parasites appeared to be degenerated and formed small vacuoles, and the tegumental ridges were also degenerated and showed somewhat flattened. In the parasites obtained from the treated rats, a different size of vacuoles interspersed in the distal part of the syncytial tegument and also in subepithelial region of the urinary bladder. These vacuoles are fused each other and lead to the disruption of the apical region of syncytial tegument along the basement layer. Finally the basement layer was dislocated. So that the tegumental layer appeared as a large balloon.
parasitology-helminth-trematoda ; Clonorchis sinensis ; chemotherapy

The therapeutic effect of praziquantel on Fibricola seoulensis infection were studied by experimental infection of albino rats with the metacercariae obtained from the snakes. Total 51 albino rats were infected each with 500 or 1,000 metacercariae in number through intragastric tubes. One or four weeks later the rats were treated with 1, 5, 10 or 20 mg/kg sigle dose of praziquantel and sacrificed after 3 days or 10-120 minutes to search for the worms in their small intestine. The worms recovery rate at 3 days after the treatment was 10.7% in 1 mg/kg does group and 0.03-0.2% in 5, 10 and 20 mg/kg group, while that of untreated control was 51.3%. The minimum effective dose to treat F. seoulensis infection in the rats is considered to be 5 mg/kg in sigle dose. By observing the distribution pattern of worms in the small intestine after the treatment, dislodgment of the worms froms the duodenum, thier normal habitat, to the lower portion was recognizable within as early as 10 minutes. The majority of worms was found to have descended to the ileo-caecal portion after 60-120 minutes.
parasitology-helminth-trematoda ; Fibricola seoulensis ; chemotherapy-praziquantel

The efficacy of reduced single doses of mebendazole against Ascaris lumbricoides infection was evaluated by cellophane thick smear and egg counting techinques, in two rural populations from December 1977 to March 1978. Total 191 infected cases were divided into five dose groups; 100 mg single dose, 200 mg single dose, 300 mg single dose, 400 mg and 600 mg in repeated doses. The cure rate of mebendazole in the treatment of Ascaris infection was remarkably high and satisfactory in every dose groups. The egg reduction rates were all over 98 percent in every dose groups. Side effects were observed during the treatment, mainly mild and transient gastrointestinal troubles, and not exacerbated by the increase of doses upto 300 mg in single dose. Above results confirmed that in case of mass chemotherapy of Ascaris infection, 100 mg or 200 mg of single dose treatment is sufficient to achieve the economic and acceptable method of treatment.
parasitology-helminth-nematoda ; Ascaris lumbricoides ; chemotherapy-mebendazole

Tinidazole (Fasigyn), a new drug which is active in vitro and in vivo against Entamoeba histolytica, was used in 30 Korean patients with acute and chronic intestinal amoebiasis. In aute amoebic dysentry eight of ten patients given tinidazole 150 mg q.i.d. for 10 days were cured clinically and parasitologically. In chronic intestinal amoebiasis, the parasitological cure was obtained 6 out of 10 patients given150 mg q.i.d. for 10 days and 8 out of 10 in patients given 300 mg q.i.d. for 7 days. Tinidazole is well tolerated and is free from serious toxicity. There was no significant alteration in blood count, liver function tests and urine analysis of any of the patients treated with tinidazole.
parasitology-protozoa-Entamoeba histolytica ; chemotherapy-Tinidazole ; drug ; Tinidazole

A study on the effect of diethylcarbamazine (DEC) (Supatonin) against Brugia malayi infection was conducted on Cheju Island in September 1965. A total of 182 persons living in a village of Aiwol Myun, Bukcheju-Gun was examined for microfilaraemia. Microscopic examination of smears of 20 microliter of blood revealed a microfilaria positivity rate of 28.5 per cent. At the end of September 1965, 34 confirmed microfilaria positive cases were treated with DEC at a daily dosage of 5 mg/kg body weight. A full course of 12 days of drug administration divided of two rounds for 6 days each was used. The first round of treatment was given under a strict supervision of the author in order to observe carefully side-effects of the drug. The second round of treatment was given in January 1966. The microfilaria density in 20 microliter of blood of those who received the drug was checked four times; before the treatment, during the first round of the treatment, 2 weeks and 4 months after the completion of the first round. The pre-treatment mean microfilaria density of 104.6 diminished to nearly zero (only two cases with one microfilaria respectively) 2 weeks after the first round and again slightly rose up to 0.5 four months after the first round. These results indicate that DEC (Supatonin) is highly effective to eliminate the microfilaria of B. malayi. However, severe side-effects, e.g. fever (average 38.6C, maximum 39.7C), headache, backache and seldom abdominal discomfort ect. were observed. There were two cases of withdrawal from the scheme due to refusal.
parasitology-helminth-nematoda ; Brugia malayi ; chemotherapy-diethyl carbamazine ; diethylcarbamazine

A series of 250 patients with filariasis due to periodic type of Brugia malayi was selected for the mass treatment with diethylcarbamazine from two areas in Cheju-Do. Two types of dosage schedules have been employed: the one was the conventional dosage schedule (uninterrupted dose of 6 mg/kg once daily 6 times repeated with an interval of one or two month totaling 72 mg/kg), and the other was the low dosage schedule (uninterrupted dose of 0.5, 1, 2, 4, 6 mg/kg body weight given once a day with an additional 6 mg/kg once daily totaling the maximum 37.5 mg/kg). The relative effectiveness of those two schedules was evaluated under the basis of the effects on microfilaria counts and the results of analysis of side-reactions observed. The results obtained in this experiment are summarized as it follows. In the conventional dosage treatment, 118 out of 141 microfilaria positives treated (83.7%) became negative and microfilaria reduction rate was about 99%. However, in the low dosage treatment, 35 out of 43 positives treated (85.4%) became negative and reduction rate of microfilaria, 99.5% in average. Total dose and duration of treatment were 72 mg/kg and about 60 days in the former schedule but it were maximum 37.5 mg/kg and 10 days in the latter. The recurrence of filariasis after treatment in both conventional and low dosage was negligible. Comparative analyses regarding the resulting side-reactions between the two schedules were made on their frequency, severity, onset and duration and relationship with microfilaria density. The febrile reaction was most frequently observed in 80.5% in the conventional, 43.9% in the low dosage schedule. The main side-reactions usually appeared within 6 to 10 hours and lasted 48 to 72 hours in the former threament, however they occurred in 7 to 8 hours and lasted 24 to 43 hours in the latter dosage schedule. It is assumed that the side-reactions are not directly related with the microfilaria count. However, the febrile reaction seems to be correlated with microfilaria density if it is above a certain limit. The presence of adult worm in a lymphnode involved with local reaction was ascertained from the biopsy specimens performed and local reactions such as lymphangitis and lymphadenitis after drug administrations were assessed from clinical and pathological point of view. It was conclusively indicated that the chemotherapeutic response to low dosage schedule within shorter length of treatment was equal to that of larger doses in long term medication from the point of the reduction in microfilaria, and particularly the resulting side-reactions were also reduced in the low dosage schedule.
parasitology-helminth-nematoda-Bruigia malayi ; chemotherapy-diethylcarbamazine ; microfilaria ; diethylcarbamazine

The effect of praziquantel on P. westermani exposed in vitro was observed by stereomicroscope, light microscope and scanning electron microscope. Following results were found. The worms incubated in 0.01 micro-g/ml praziquantel were moving after 26-hour incubation. However, all of them were immobilized immediately after incubation in solutions over 0.1 micro-g/ml concentration. All of the exposed worms showed severe vacuolization not only in tegument but in subtegument, intestine, ovary, testis, Mehlis' gland and excretory bladder. Vacuoles in tegument burst out to form craters. As incubation time went on, tegumental structure was disintegrated severely. The worms exposed to praziquantel were observed to be immobilized and be vacuolized of all tissues. Disintegration of reproductive organs suggests that praziquantel have suppressive effect on egg production when the flukes are not killed. The drug effects were found more related with incubation time than with drug concentration.
parasitology-helminth-trematoda ; Paragonimus westermani ; electronmicroscopy ; chemotherapy ; praziquantel