The distribution of glycogen, polysaccharide, mucopolysaccharide, lipid and nucleic acid has been studied in Echinorhynchus gadi(Acanthocephala). The results were summarized as follows: Glycogen and polysaccharide was demonstrated by Bauer PAS reaction technique and was found in fertilization membrane in ovum, central nuclear mass in acanthor and lemnisci, hypodermis in cystacanth. Mucopolysaccharide was demonstrated by Mowry alcian blue staining technique and was found in outer membrane, fibrillar coat, fertilization membrane and inner membrane in acanthocephalan ova. Lipid was demonstrated by Smith Nile blue stain and Lison Sudan black B staining technique and was found roughly parallel to that of polysaccharide. Nucleic acid was demonstrated by Rosenbeck Feulgen reaction, Taft methylgreen-pyronin stain and Diengdoh acridine orange staining technique and found in central nuclear mass in acanthor, also, was found in lemnisci, proboscis and hypodermis in cystacanth.
parasitology-Acanthocephala ; histochemistry ; Echinorhynchus gadi ; glycogen ; mucopolysaccharide ; lipid ; nucleic acid

To identify acanthocephala found in 'Changran-pickles' and 'Myungran-pickles' each organ was measured in permanent slides. In the present report, the results obtained were as follows: 1. Morphology of male worms: Worms possessed 18-19 longitudinal rows, with 4 hooks per row, which became smaller towards the base of proboscis. Each worm contained two testis and six cement glands arranged linearly. Body 22.0 by 0.8-0.6 mm and 15.0 by 0.6-0.4 mm, proboscis 284.8 by 227.6 microgram and 524.9 by 151.4 microgram, proboscis sheath 1570.7 by 72.7 microgram and 751.9 by 280.4 microgram, lemnisci length 2566.7 and 1085.6, testis 2202.9-1860.5 by 737.0-575.7 microgram and 1033.8-981.1 by 463.1-351.6 microgram, cement glands 940.2 by 441.2 microgram and 610.0 by 369.1 microgram. 2. Morphology of female worms: Worms possessed 14-18 longitudinal rows, with 6-10 hooks per row and become smaller toward the base of proboscis. Each worm contained an uterine bell and uterus in the posterior portion and the eggs filled the body cavity. Body 14.0~51.0 mm by 0.7-0.5~2.2-1.4 mm, proboscis 466.1-268.9 microgram by 259.9-252.0 microgram, proboscis sheath 1550.7-506.0 by 298.8-231.1 microgram, lemnisci length 1325.7-473.1 microgram, eggs 112.4 by 28.5 microgram~51.7 by 14.0 microgram. In this present study, the acanthocephala collected in 'Changran-pickles' and 'Myungran-pickles' were identified as Echinorhynchus gadi by morphological features.
Acanthocephala/anatomy & histology/classification/*isolation & purification ; Animals ; Female ; Fish Diseases/*parasitology ; Fishes ; Helminthiasis, Animal/*parasitology ; Korea ; Male ; Seafood/*parasitology

For the purpose of observing ultrastructure of the epicuticle of Echinorhynchus gadi, the present electron microscopical studies had been made. Also the histochemical methods of Morwy, Bauer, Smith, Lison, Taft, and those of lead and uranyl acetate had been used in order to see the distribution of glycogen, mucopolysaccharides, lipid and nucleic acid in the cuticle of Echinorhynchus gadi. The results obtained by the above studies were as follows: Glycogen, mucopolysaccharides were found in the outermost, middle and inner layers, especially abundant in the middle layer of the cuticle. Lipid was found in the middle and inner layer, and it was found abundantly around the lacunal canal in the cuticle. Nucleic acid was found around the lacunal canal in the middle layer, and also distributed in the cell nucleus of inner layer in the cuticle. Electron microscopically, the cuticle of Echinorhynchus gadi had three outer layers, being outermost, middle and inner ones. The outermost layer was medium electron dense, composed with plasmalemma and filaments. The middle layer was homogeneous one which was electron pale. The inner layer, which was electron dense, consisted of felt layer and radial layer. The electron dense glycogen, lipid granules were distributed in radial layer.
parasitology-Acanthocephala ; Echinorhynchus gadi ; electron microscopy ; histochemistry ; glycogen ; mucopolysaccharide ; lipid ; nucleic acid

Acanthogyrus (Acanthosentis) tilapiae parasites were collected from the intestines of 300 fish belonging to three tilapia species sourced at the River Nile, Giza, Egypt. The proboscis of the parasite was characterized by three rows of hooks that curved towards the posterior of the body. The first row is supported by unmodified hooks. The parasite tegument has a series of alternative folds and a large number of pores. Sensory ganglia are located on the surface of the proboscis and body. Acanthogyrus (Acanthosentis) tilapiae provokes an aggressive host response indicated by hyperplasia of the intestinal goblet cells and focal eosinophil infiltrations. This acanthocephalan parasite shows a highly modified adaptation to its site of host infection.
Acanthocephala/*anatomy & histology/*physiology/ultrastructure ; Adaptation, Physiological ; Animals ; *Cichlids ; Egypt ; Female ; Fish Diseases/*parasitology ; Histocytochemistry/veterinary ; Intestines/parasitology ; Male ; Microscopy, Electron, Scanning/veterinary

The present study was performed to investigate the infection status with helminth in a group of feral cats in Korea. More than 29 helminth species including adults or eggs were detected in visceral and fecal samples of the examined cats. Among these were a host of nematodes, including toxocarids, Ancylostoma sp. and the larva of Anisakis simplex; trematodes, including Clonorchis sinensis, Paragonimus westermani, Eurytrema pancreaticum, Pharyngostomum cordatum, Metagonimus spp., Heterophyes nocens, Pygidiopsis summa, Heterophyopsis continua, Stictodora fuscata, Stictodora lari, Acanthotrema felis, Stellantchasmus falcatus, Centrocestus armatus, Procerovum varium, Cryptocotyle sp., Echinostoma revolutum, Echinostoma hortense, Echinochasmus japonicus, Stephanoprora sp., Plagiorchis muris, Neodiplostomum sp. and diplostomulum. We also detected a variety of cestodes, including Spirometra erinacei, Taenia taeniaeformis and unidentified species of tapeworm. We also found examples of the acanthocephalan, Bolbosoma sp. In our assessment of the stools, we detected at least 12 species of helminth eggs. These findings confirmed that feral cats in Korea are infected with a variety of helminth parasite species. Furthermore, among the helminths detected, E. pancreaticum, S. fuscata, S. lari, A. felis, S. falcatus, C. armatus, P. varium, Cryptocotyle sp., E. revolutum, E. japonicus, Stephanoprora sp., P. muris, Neodiplostomum sp. and Bolbosoma sp. represent helminth fauna which have not been reported previously in feral cats in the Republic of Korea.
Acanthocephala/*isolation & purification ; Animals ; Cat Diseases/*parasitology ; Cats/*parasitology ; Cestoda/*isolation & purification ; Feces/parasitology ; Helminthiasis, Animal/*parasitology ; Korea ; Nematoda/*isolation & purification ; Parasite Egg Count ; Species Specificity ; Trematoda/*isolation & purification