OBJECTIVE:To probe into the status quo and the tendency of the utilization of psychotropic drugs in our hospital. METHODS:The consumption sum,DDDs and the daily drug cost of psychotropic drugs from 2006 to 2008 were analyzed statistically in this retrospective study. RESULTS & CONCLUSION:The consumption sum of psychotropic drugs in our hospital assumed year-on-year increase tendency. The DDDs of the first generation of psychotropic drugs remained stable. The consumption amount of the non-first generation of antipsychotic drugs increased year on year,with risperidone showing the most rapid increase. Fluoxetine,venlafaxine and paroxetine took the lead among all the antidepressant drugs used. However,the consumption amount of tricyclic antidepressants decreased markedly. Benzodiazepine took the lead among all the antianxiety drugs.

OBJECTIVE: To study the bioequivalence of azihromycin capsules in healthy volunteers. METHODS: 21 healthy volunteers designed by latin square were divided into group A, B and C. The three groups were given single dose of azihromycin capsules 1(0.25 g in every capsule), azihromycin capsules 2(0.125 g in every capsule) and reference preparation(0.25 g in every capsule). The plasma concentrations of azihromycin were determined by microbiological assay and pharmacokinetic parameters were calculated and the bioequivalence was evaluated. RESULTS: Main pharmacokinetic parameters of three kinds of preparations were as follows:Cmax(1.06?0.41)?g?mL-1,(0.99?0.54) ?g?mL-1 and (1.07?0.53) ?g?mL-1; tmax(2.38?0.86)h, (1.86?0.85)h and (2.52?1.40)h; AUC0～144(11.00?6.15)?g?h?mL-1,(10.65?5.17) ?g?h?mL-1 and (10.49?5.17)?g?h?mL-1;AUC0～∞(12.48?7.27)?g?h?mL-1,(11.65?5.64)?g?h?mL-1 and (11.57?5.87)?g?h?mL-1. The relative bioavailabitity of azihromycin capsules 1 and azihromycin capsules 2 were (102.73?15.63)% and (103.17?13.64)%. CONCLUSION: The results suggest that two specifications of azihromycin capsules are bioequivalent to reference preparation.

Chronic Disease ; Elasticity Imaging Techniques ; Humans ; Liver Diseases ; diagnostic imaging

Objective:To study the effects of PLCepsilon siRNA transfection on invasive power of human bladder cancer cell lines T24,and to bring a new method of gene therapy for bladder cancer.Methods:An eukaryotic expression vector containing PLCepsilon siRNA was transfected into T24 and positive cell clones were selected and amplified.Expression of PLCepsilon mRNA was detected by RT-PCR.invasive power of T24 were measured before and after transfection by the membrane invasion culture system(Transwell chamber)and gelatin enzymography(including 72 ku typeIV collagenase and 92 ku typeIVcolagenase analyzed).Results:the result of RT-PCR display that the T24 transfected with pll-PLCepsilon and p12-PLCepsilon vectors had lower expressing of PLCepsilon mRNA than the control groups T24 transfected with HK-A and T24 without transfection.In the membrane invasion culture system the invasion number of T24 transfected with p12-PLCepsilon(26.8?5.8)and the number of T24 transfected with pll-PLCepsilon(25.8?6.2)are both lower than the the control groups T24 transfected with HK-A(33.8?5.7)and T24 without transfection(34.8?6.9)(P

0.05).Conclusions:1.The acute rise in FFAs level for a short term can inhibit ACTH and cortisol secretion in healthy male adults,but with prolonged FFA elevation this inhibition last less than 24 hours.2.The acute FFAs rise has no influence on ACTH or cortisol circadian rhythm.

Objective:To discuss the surgical management of spondylodiscitis after the lumbar discectomy.Methods:7 cases of spondylodiscitis after the lumbar discectomy were analysed retrospectively.All patients were treated with surgical management of posterior approach combined with antibiotics.Results:In 7 patients,4 were males and 3 females.The mean age was 45 years old.The spinal segment involved included L 4/5 in three and L 5S 1 in four cases.The history, clinical courses,ESR and MRI are very useful in the diagnosis of postoperative spondylodiscitis.3 cases presented positive in the bacterial culture and 4 cases negative.After the surgery of the posterior focal debridement and bone plantation with internal fixation, the symptoms were relieved in a short period of time.The roentgenographic followe-up showed the spinal fusion 2.5～3.5 months after surgery.Conclusion:The surgical management of the posterior approach has more advantages over the anterior approach.It is one of the most effective methods for treatment of the spondylodiscitis after lumbar discectomy.

Objective:To evaluate the clinical efficacy of radical debridement and spinal fusion with internal fixation in surgical management of cervical spinal tuberculosis with kyphosis deformity.Methods:There were 14 cases in this series.The mean age was 32 years.14 cases of cervical spinal tuberculosis were radical focus debrided and grafted with iliac bone.5 cases were fixed with Orion,4 cases with Zephir,4 cases with Codman and 1 case with Apofix.The brace was needed for 3 months after surgery.Results:The period of follow-up lasted 21 months.All patients achieved satisfactory results,and showed successful interboby fusion within 3～6 months.The kyphosis deformity were corrected and the normal cervical lordoisis were maintained without associated complication.The neural symptoms were improved.All patients had no recurrence of tuberculosis.There were no plates and screws complications.Conclusion:It is safe and effective to treat cervical spinal tuberculosis by anterior radical debridement with primary autograft and internal fixation,and it is a good treatment option with high correction rate in local kyphosis deformity,high fusion,and low complication rate.

Objective:To investigate the effects of Urocortin-postconditioning on mitochondria-dependentapoptotic pathway.Methods: 24 Wistar rats were randomly divided into 3 groups(n=8):Control group(CON),I/R group(I/R),Urocortin group(UCN).The left ventricular samples in the Control group were collected as pre-ischemia controls through thoracotomy.After isolated heart Langendoff and Neely models were established,the rat hearts in the I/R group and the UCN group were continuously perfused with Krebs-Henseleit (K-H)buffersolution for 30 min,then the rat hearts were arrested by cardioplegia,St.Thomas-2 solution(STH-2)for 30min.After that,the rat hearts in the I/R group were reperfused with K-H buffer solution for 90 min.The rat hearts in the UCN group were reperfused with K-H buffer solution containing 10-8 mol/L Urocortin for 90 min.The releasing of Cyto C protein into cytosol was detected by Western blot.The expression of Caspase-3 protein in cardiomyocyte was detected by immunohistochemical assay.The apoptosis index (AI)of cardiomyocyte was detected by TUNEL.Results:Compared with Control group,the expression ofCaspase-3 and Cyto Cprotein in the I/R group and the UCN group were significantly increased(P

Glucose-6-phosphate dehydrogenase deficiency is a common monogenic inheritance disorder, with extensive distribution around the world and very high gene frequency. Acute hemolysis may lead to rapidly rising of serum total bilirubin concentrations and may eventually result in bilirubin encephalopathy.The hyperbilirubinemia is the result of abnormal bilirubin metabolism due to complex interactions between G-6-phosphate dehydrogenase deficiency and the genetic co-expression. Neonatal unconjugated hyperbilirubinemia is one of the most common conditions encountered by the practicing pediatricians.

Objective: To study the practical value of a new techinigue of perforating vein ligation. Methods: Enlightened by Subfascial Endoscopic Perforator, we isolate perforating vein in subfasica through little incision, and then ligate and cut perforating vein. The change of the symptoms and signs were observed, and the clinical severity grade of pre-operation were compared with that of post-operation. Results: The symptoms and signs of patients are improved after operation, and the clinical severity grade of postoperation of the patients was significant different, comparing with that of pre-operation. Conclusion: The effect of perforating vein ligation through little incision at subfascia is effective and its complications are few.