Chronic Disease ; Elasticity Imaging Techniques ; Humans ; Liver Diseases ; diagnostic imaging

Objective:To study the solubilization effect of 2-hydroxypropyl-?-cyclodextrin(2-HP-?-CD) on anethol trithione and the complexation of anethol trithione with 2-HP-?-CD.Methods:On the basis of single factor experiment,the drug complexed rate was adopted as the index,to study the effects of factors like host-guest molar ratio,alcohol concentration,stiring rate and stiring time on the techniques of preparing anethol trithione-2-HP-?-CD;the inclusion complex was identificated and solubility was detected by diferential scanning calorimeter(DSC) methods.Results:2-HP-?-CD can obviously enhance solubility of anethol trithione.Conclusion:The solubility of inclusion complex increased by 460 times when it was included by 2-HP-?-CD.

Objective:To investigate the effects of Urocortin-postconditioning on mitochondria-dependentapoptotic pathway.Methods: 24 Wistar rats were randomly divided into 3 groups(n=8):Control group(CON),I/R group(I/R),Urocortin group(UCN).The left ventricular samples in the Control group were collected as pre-ischemia controls through thoracotomy.After isolated heart Langendoff and Neely models were established,the rat hearts in the I/R group and the UCN group were continuously perfused with Krebs-Henseleit (K-H)buffersolution for 30 min,then the rat hearts were arrested by cardioplegia,St.Thomas-2 solution(STH-2)for 30min.After that,the rat hearts in the I/R group were reperfused with K-H buffer solution for 90 min.The rat hearts in the UCN group were reperfused with K-H buffer solution containing 10-8 mol/L Urocortin for 90 min.The releasing of Cyto C protein into cytosol was detected by Western blot.The expression of Caspase-3 protein in cardiomyocyte was detected by immunohistochemical assay.The apoptosis index (AI)of cardiomyocyte was detected by TUNEL.Results:Compared with Control group,the expression ofCaspase-3 and Cyto Cprotein in the I/R group and the UCN group were significantly increased(P

Many chemokines and chemokine receptors, such as CCL2 and CXCR4, participate in the growth, angiogenesis, distal metastasis of breast cancer. However, the recent failures in the clinical trials of some single target antagonists suggest that chemokine network in tumor microenvironment is really complicated. Obviously, multi-targets regulation strategy for chemokines is indispensable based on the polypharmacological principle in the future. Atypical chemokine receptors (ACR) as endogenous and physiological regulators, including Duffy antigen receptor for chemokines (DARC), D6 and ChemoCentryx chemokine receptor (CCX-CKR), may be the powerful candidates for this purpose.

Objective:To evaluate the clinical efficacy of radical debridement and spinal fusion with internal fixation and to discuss the safety of anterior instrumentation in surgical management in thoracolumbar spinal tuberculosis.Methods:There were 16 cases in this series.The mean age was 45 years.16 cases of thoracolumbar spinal tuberculosis were radical focus debrided and grafted with iliac bone.8 cases were fixed with TSRH,3 cases with Ventrofix and 5 cases with Z-plate.No brace was needed after surgery.Results:The period of follow-up lasted 19 months.All patients had good results.The incision of all cases were cured for the first term.No case had sinus.13 degree of the kyphosis deformity was improved without associated complication.All patients had no recurrence of tuberculosis.Conclusion:The results show that the radical focal debridement and anterior bone transplantation with internal fixation can reconstruct the spinal stability and it is safe to implant anterior instrumentation in tuberculosis foci.

Objective:To discuss the surgical management of spondylodiscitis after the lumbar discectomy.Methods:7 cases of spondylodiscitis after the lumbar discectomy were analysed retrospectively.All patients were treated with surgical management of posterior approach combined with antibiotics.Results:In 7 patients,4 were males and 3 females.The mean age was 45 years old.The spinal segment involved included L 4/5 in three and L 5S 1 in four cases.The history, clinical courses,ESR and MRI are very useful in the diagnosis of postoperative spondylodiscitis.3 cases presented positive in the bacterial culture and 4 cases negative.After the surgery of the posterior focal debridement and bone plantation with internal fixation, the symptoms were relieved in a short period of time.The roentgenographic followe-up showed the spinal fusion 2.5～3.5 months after surgery.Conclusion:The surgical management of the posterior approach has more advantages over the anterior approach.It is one of the most effective methods for treatment of the spondylodiscitis after lumbar discectomy.

Objective:To evaluate the clinical efficacy of radical debridement and spinal fusion with internal fixation in surgical management of cervical spinal tuberculosis with kyphosis deformity.Methods:There were 14 cases in this series.The mean age was 32 years.14 cases of cervical spinal tuberculosis were radical focus debrided and grafted with iliac bone.5 cases were fixed with Orion,4 cases with Zephir,4 cases with Codman and 1 case with Apofix.The brace was needed for 3 months after surgery.Results:The period of follow-up lasted 21 months.All patients achieved satisfactory results,and showed successful interboby fusion within 3～6 months.The kyphosis deformity were corrected and the normal cervical lordoisis were maintained without associated complication.The neural symptoms were improved.All patients had no recurrence of tuberculosis.There were no plates and screws complications.Conclusion:It is safe and effective to treat cervical spinal tuberculosis by anterior radical debridement with primary autograft and internal fixation,and it is a good treatment option with high correction rate in local kyphosis deformity,high fusion,and low complication rate.

BACKGROUND:Silver sulfadiazine water gel dressings are a combination of two antibacterial drugs, which can play a local broad-spectrum, potent, long-lasting antibacterial effect to effectively control and prevent wound infection. OBJECTIVE:To observe the clinical efficacy of silver sulfadiazine water gel dressings on skin defects with wound infection and to explore its biocompatibility. METHODS:Thirty patients with skin defects combined with wound infection were randomized into observation and control groups. Folowing conventional debridement and dressing, the observation group was subject to wound rinse with normal saline, silver sulfadiazine water gel dressings and gauze bandage; in the control group, Vaseline gauze was used folowed by gauze bandage. Visual analog scale scores, dressing adhesion to wound, wound healing rate, and healing time were observed in the two groups at 0, 1, 2 weeks after dressing. RESULTS AND CONCLUSION:The visual analog scale scores in the observation group were significantly lower than those in the control group at 1 and 2 weeks after dressing (P < 0.01), and the wound healing rate was also higher in the observation group than the control group (P < 0.05); wound adhesions were milder in the observation group than the control group when dressing; the healing time was significantly shorter in the observation group than the control group (P < 0.05). These findings suggest that the silver sulfadiazine water gel dressing has good effects on the treatment of skin defects with wound infection, which can reduce pain, effectively control wound infections and promote wound healing.

Objective To explore the relationship of recurrence rate and the age and body temperature of fitrst febrile seizures (FS) in children.Methods One hundred and ninety-two cases of FS admitted to our hospital from Jan 2004 to Jun 2006,were divided into three groups according to the initial onset of body temperature with FS:＞40.0 ℃,～40.0 ℃,≤38.5 ℃ ;three groups according to the duration between fever initiation and seizure attack:≤6 h,～ 12 h,～24 h,＞24 h;four groups according to age:≤6 months,～ 18 months,～ 3 years,＞ 3 years.For each group of children with FS,the recurrence rate and follow-up were compared.Results All cases were followed up to 6 years old,22 cases were lost,and the follow-up rate was 88.5％ (170/192).Follow-up of 170 children with FS,the recurrence rate was 32.6％ (15/46),51.1％ (46/90) and 79.4 ％ (27/34) in each group of temperature ＞ 40.0 ℃,～ 40.0 ℃,and ≤ 38.5 ℃.There were statistically significant among three groups (x2 =17.18,P ＜ 0.05).Duration of fever before seizures ≤ 6 h group,～24 h group,＞ 24 h group,the recurrence rate were 70.7 ％ (29/41),51.6％ (49/95) and 29.4％ (10/34) respectively,the recurrence rate was significant difference among the three groups(x2 =12.71,P ＜0.05).The recurrence rate of different age groups in initial attack of FS were 84.6％ (22/26),63.4％ (45/71),39.5％ (15/38),17.I％ (6/35),the differences were statistically significant (x2 =34.17,P ＜ 0.05).Conclusion There was higher recurrence rate of febrile seizure in small children,lower body temperature and shorter duration between fever initiation and seizure attack.