[Objective] Sorting and summing up Professor ZHANG 's clinical experience in the diagnosis and treatment of atlanto-axial joint subluxation, to develop the thought of clinical diagnosis and treatment of the disease. [Methods]Via studying with professor ZHANG, and collectting some cases,consulting the related literature and data.Finally, discuss and summarize it. [Results] Diagnosis found and appliedFour tenderness(Below the foramen magnum, trapezius muscle and at the junction of the skull,fengchi point,wangu point).Method combined with primary symptom and signs, X-ray. Treatment, application of error correction manipulation, light effect;And with local acupuncture point injection, menstruation invigorates the circulation of reinforcement. [Conclusion] Professor ZHANG's method of diagnosis and treatment of atlanto-axial joint subluxation is unique,clear, simple, has obvious curative effect, worth clinical application and promotion.

Hypoxia- inducible factor-1 ( HIF-1) promotes tumor deterioration, invasion and metasta-sis. Treatment targeting the HIF-1 signal pathway is becoming a hot research. In addition to uniquely selective inhibition of topoisomerase Ⅰ(Topo Ⅰ) ,the recent studies show that camptothecin compounds can regulate the activity of HIF-1 through decreasing HIF-1 protein translation and have been classified as a non-selective chemical inhibitor of HIF-1.

Sphingosine analogue FTY720 exerts good immune suppressive effect by inducing lymphocytes apoptosis and homing, which can be used for organ transplant rejection as well as anti-autoimmune disease treatment. Recent studies have found that FTY720 plays anti-tumor potential by inducing growth arrest and apoptosis as well as inhibiting tumor metastasis.

AIM:To establish a method for determining notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Jingkang Capsules(Radix Rehmanniae praeparata,Radix Polygoni multiflori,Radix et Rhizoma Notoginseng,etc.). METHODS: A Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m) with acetonitrile-water as the mobile phase was used for gradient elution,the flow rate was 1.0 mL/min and the UV detection wavelength was at 203 mn. RESULTS: There was a good linear relationship at a range of 0.327-2.180 ?g for notoginsenoside R_1(r=(0.999 4))(the average recovery was 98.23% with RSD of 1.53%(n=6)),0.909-6.060 ?g for ginsenoside Rg_1(r=1.0)(the average recovery was 99.04% with RSD of 1.00%(n=6)),0.672-4.480 ?g for ginsenoside Rb_1(r=0.999 8)(the average recovery was 98.17% with RSD of 1.31%(n=6)).CONCLUSION: The method is simple,quick and accurate.It can be used for the quality control of Jingkang Capsules.

The physical change of astigmatism in human eye is analyzed in this article.Different methods for astigmatism applied in clinic are expound and compared with advantages and disadvantages.It is important that analyzing carefully and designing customized normgram for astigmatism eye to approach the perfect visual function after reasonable treatment.

DNA extraction is a basic technology of molecular biology. The purity and the integrality of DNA structure are necessary for different experiments of gene engineering. In recent years there have been some new or improved DNA extraction methods appeared. The methods of DNA extraction from animals, plants, microorganisms and marine organisms were summarized in this article.

Objective To investigate the related factors and preventive measures of nasopharyngeal massive hemorrhage in patients with nasopharyngeal carcinoma after radiotherapy. Methods Clinical data of 43 nasopharyngeal massive hemorrhage patients with nasopharyngeal carcinoma after radiotherapy were analyzed retrospectively. Results Thirty-nine cases(90.7 %) had abroad skull base invasion due to tumor, 36 cases (83.7%) received high dose of radiation, and 13 cases (30.2 %) were accompanied by diabetes. Conclusion Tumor location is the major factor for nasopharyngeal massive hemorrhage, while radiation dosage, fractionation patterns and diabetes are the secondary factors. The measures in cluding hyperfractionation, overall dose control and blood sugar regulation should be taken for the high risk patients. Prevention is more important than salvage.

Objective:To study the transdermal permeability of Psoralen (Pro) and Isopsoralen (Isopro). Methods:V C two compartment was adopted to research the permeability deferences between Pso and Isopso in nude mouse skin in vitro, and investigated the effect of penetration enhancers, such as EtOH, azone and glycerol on the permeability of them. Results: Pso and Isopso are suitable compounds for transdermal delivery system and the permeability coeffient of Isopso is larger than that of Pso. 10% EtOH-1% Azone greatly increased the permeability coefficient. Conclusion: The results can offer reference for Psoralea corylifolia TDS.

Developing changes of physiology and biochemistry in myocardium and brain tissue after myocardial infarction in rats was observed. The result showed that BP、 ?dp/dtmax and BBF were dccreased significantly at 6 hours after ischemia. The BBF was returned to normal after 72 hours but BP and ?dp/dtmax wcrc kept lower for 7 days. The MDA was clearly higher at 72 hours and returned to normal at 7th days in myocardium and brain tissue. BUt the NAGase reached to the peak at 7th days and was returncd to normal at 14th days. The changes of MDA and NAGase were correlated to the changes of heart fuetion after myocardial infarction. It suggested that cardiogcnic cerebral ischemia is a real fact.

Objective To investigate the coagulation and fibrinolytie system changes in liver cirrhosis (LC) patients with and without portal vein thrombosis (PVT). Method Twenty-six cases of LC with PVT (PVT group) and 61 eases of LC without PVT (control group) were chosen. Platelet(Pt), prothrpmbin time (PT), activated partial thromboplastin time (APTT), fibrin (Fib), D-dimer, tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-l) were determined. SPSS 11.5software was used for statistic analysis. Results In PVT group, the average level of D-dimer was (0.97±0.53 ) mg/L,signifieantly higher than that in control group (0.61±0.40) mg/L. No significant difference was found between the two groups in Pt PT, APTT, Fib, t-PA and PAI-1. PT and APTT were progressively prolonged from Child-Pugh grade A to B and then to C. Fib was progressively decreased from Child-Pugh grade A to B and then to C. Decreased Pt and increased t-PA, PAI-1 were found in LC patients, but had no sighifieant difference. Conclusions The changes determined of coagulation and fibrinolytie system in patients with LC are abnormal. The average level of D-dimer is higher in LC patients with PVT than that in LC patients without PVT. D-dimer may be a helpful tool to rule out the presence of an underlying PVT in LC patients.