OBJECTIVE: To study the effects of Semen Litchi on the expressions of S180 and EAC tumor cells and Bax and Bcl-2 proteins in rats. METHODS: EAC, S180 and hepatic carcinoma tumor-bearing mice models were established in mice by inoculating them with tumor strains. EAC, S180 and hepatic carcinoma suspensions were diluted respectively with normal saline in a ratio of 1∶1 for the preparation of tumor-bearing ascites suspension. Each rat was subcutaneously inoculated with the mixture 0.2 mL via axilla 24 hours before medication, with the whole sterile operation finished within 60 minutes. After treated with Semen Litchi for 10 consecutive days, the growth of EAC, S180 and hepatic carcinoma in mice was observed. RESULTS: The growth of EAC and S180 and hepatic carcinoma cells in mice was significantly inhibited by Semen Litchi. CONCLUSION: Semen Litchi can inhibit the growth of S180 and EAC tumor cells in vitro and in vivo, and its antitumor effect is related to its ability to promote apoptotic of tumor cells.

OBJECTIVE: To study the effects of extracts of the seed of Litchi (ESL) on the expression of estradiol receptor (ER) and progestogen receptor (PR) in mammary tissue and apoptosis of mammary glandular cells in rats with hyperplasia of mammary glands (HMG). METHODS: The HMG model was induced by intramuscularly injecting with 0.5 mg?kg-1 estradiol benzoate for 25 days, subsequently intramuscularly injecting with 4 mg?kg-1 progesterone for 5 days. 60 healthy nonfertile SD rats were divided into normal group, model group, rujieping group and high-dose, medium-dose and low-dose ESL groups, treating with distilled water, distilled water, 3.3 g?kg-1 Rujieping and 44, 22, 11 g?kg-1 ESL via i.g. gtt once a day for 30 days, respectively. Immunohistochemical method was applied to observe the expression of ER and PR in rat mammary tissue and apoptosis of mammary glandular cells. Uterus index was also calculated. RESULTS: High-dose and medium-dose of ESL can inhibit the expression of ER and PR of mammary gland in HMG rats and induce apoptosis of mammary glandular cells(P

Diabetes Mellitus ; drug therapy ; Diabetic Angiopathies ; drug therapy ; Diagnosis, Differential ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Hypoglycemic Agents ; therapeutic use ; Medicine, Chinese Traditional ; Phytotherapy

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OBJECTIVE: To explore the antioxdant action of Xiangsha liujun pill in hyperlipidemia model rats. METHODS: Hyperlipidemia model was established by intragastric administrating emulsion in rats. The levels of blood lipids, serum SOD activity and MDA content in rats before and after intragastric administration of Xiangsha liujun pill were measured. RESULTS: Xiangsha liujun pill markedly inhibited the concentrations of serum total cholesterol (TC), trglyceride (TG), low-density lipoprotein (LDL) and high-density lipoprotein (HDL), promoted the activity of SOD and decreased the content of MDA (P

OBJECTIVE:To establish an LC-MS method for the determination of the ritodrine content in human plasma.METHODS:The determination was performed on Agilent Eclipse Plus C18 column with the column temperature kept at 40 ℃.The mobile phase consisted of methanol-water(95∶5,containing 0.02 mol?L-1 ammonium formate)with a flow rate of 0.5 mL?min-1.The extraction agent was acetic ether-dichloromethane(4∶1).A triple quad mass spectrometer system equipped with an electro spray ionization ion-trap source was used as the detector and operated in the positive ion model.Selected reaction monitoring(SRM)was employed to detect ritodrine(m/z 288.2→121.1)and the internal standard dobutamine(m/z 302.1→107.0).RESULTS:The linear range of ritodrine was 0.25～100 ng?mL-1(r=0.999 1),with the lower limit of quantification at 0.25 ng?mL-1.Both the intra-day RSD and inter-day RSD were less than 15%.The methodological recovery was 98.00%～103.44%.CONCLUSION:The method is sensitive,accurate,reproducible,specific and suitable for the pharmaceutical study of ritodrine.

The JCI-based PDCA cycle makes continued quality improvement its comerstone.Its use in ICU human resource management consists of assurance of HR quantity,assurance of HR quality,and scientific use of human resources.Its application in ICU nursing care consists of creating excellent environment and providing high quality nursing items,with emphasis on quality nursing service in detail.Conversion of working mode,rationalized HR deployment,high quality nursing care,all contribute to raising the quality and social reputation of the hospital and the department,to standardized management,enhanced teamwork,better nursing care quality,and to higher satisfaction of the patients in the end.

OBJECTIVE: To investigate the clinical features of fungal rhino-sinusitis. METHOD: Clinical data of 189 patients suffering from fungal rhino-sinusitis treated by functional endoscopic sinus surgery was analyzed retrospectively. The analyzed data included clinical type, age of onset, predilectionsite, reason, and surgical outcome. RESULT: Among the 189 patients with fungal rhino-sinusitis,181 cases were fungal ball,6 cases were allergic fungal rhino-sinusitis, 1 case was acute invasive fungnal rhino-sinusitis, 1 case was chronic invasive fungnal rhino-sinusitis. One hundred and twenty-eight cases were in the maxillary sinus (123 cases were unilateral, 5 cases were bilateral). Nineteen cases were in the ethmoid sinus. 31 cases were in the sphenoid sinus. Two cases were both in the maxillary sinus and ethmoid sinus, 1 case was both in the maxillary sinus and sphenoid sinus. Two cases invasive fungnal rhino-sinusitis had diabetes history. All the patients treated by functional endoscopic sinus surgery, 184 cases without recurrence, 5 cases suffered re-operation. CONCLUSION The incidence of fungal rhinosinusitis is showing a rising trend, fungal ball is the highest. The sinusitis patients whom we highly doubt for fungal infection should be confirmed by using sinonasal secretion smear, cultivation and histopathological examination. Surgical treatment should completely remove the fungal masses, to avoid reoperation.
Chronic Disease ; Emotions ; Ethmoid Sinus ; pathology ; Fungi ; Humans ; Maxillary Sinus ; pathology ; Mycoses ; pathology ; surgery ; Recurrence ; Retrospective Studies ; Rhinitis ; microbiology ; surgery ; Sinusitis ; microbiology ; surgery ; Sphenoid Sinus ; pathology

Objective To assess the diagnosis value of epithelial membrane antigen (EMA) immunocytochemistry examination in meningeal carcinomatosis.Methods The routine cerebrospinal fluid cytologic examination and EMA immunocytochemistry examination of 23 cerebrospinal fluid specimens from 14 patients with definitive meningeal carcinomatosis were analyzed,retrospectively.Results The positive rate of routine cerebrospinal fluid cytologic examination and EMA immunocytochemistry examination were 39.13%(9/23)and 86.96%(20/23),respectively. There was a significant difference between two results ( P

Diabetic peripheral neuropathy (DPN) is a common complication of diabetic mellitus. The treatment is so difficult that doctors have not found reliable methods to cure this disease. The research progress both in TCM and western medicine of this disease were reviewed in terms of aspects of etiology, pathogenesis and differential treatments.