1.Effects of Chinese herbal medicine Shenlong Decoction on mRNA expressions of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 in lung tissue of rats with pulmonary fibrosis induced by bleomycin.
Xiaodong LU ; Lijian PANG ; Linlin WANG ; Minghua NAN ; Zhi MA
Journal of Integrative Medicine 2010;8(10):961-7
To observe the expressions of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in rats with pulmonary fibrosis (PF) induced by bleomycin, and to explore the mechanisms of Shenlong Decoction in preventing and treating PF.
2.Effects on Salvia miltiorrhiza hairy roots of tanshinones content accumulation after treated with fosmidomycin.
Xia-Nan ZHANG ; Zhi-Shuang WANG ; Wei GAO ; Lu-Qi HUANG
China Journal of Chinese Materia Medica 2013;38(24):4263-4266
Fosmidomycin (100 micromol x L(-1)) which is the effective inhibitor of DXR, key enzyme in terpenoid MEP pathway, was used to treat with hairy roots of Salvia miltiorrhiza. The treated roots were harvested at 2, 4, 6, 8, 10, 16 and 21 d, mRNA level of SmDXR and tanshinone content in treated and negative control groups were detected. Results found that, after treated with fosmidomycin, color of S. miltiorrhiza hairy roots grew pale gradually comparing with controls; mRNA level of SmDXR in hairy roots varied as a shape of parabolic and the highest value achieved at the sixth day after treatment, then it decreased gradually; Content of four kinds of tanshinones were detected. Among of the four kinds of tanshinones, Tanshinone I content changed relatively little, while content of dihydrotanshinone I, cryptotanshinone and tanshinone II (A) decreased gradually in 21 days. The content of total tanshinones in NC groups was 5, 63 times more than FOS-treated roots in the 21th day. The previous results showed that SmDXR played an important role in the accumulation of tanshinone content in MEP pathway. Once the mRNA level of SmDXR was suppressed, the accumulation of secondary metabolites will be significantly affected.
Aldose-Ketose Isomerases
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genetics
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Diterpenes, Abietane
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metabolism
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Fosfomycin
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analogs & derivatives
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pharmacology
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Gene Expression Regulation, Plant
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drug effects
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Plant Roots
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drug effects
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growth & development
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metabolism
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RNA, Messenger
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genetics
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metabolism
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Salvia miltiorrhiza
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drug effects
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genetics
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growth & development
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metabolism
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Time Factors
3.Comparison of two methods for evaluation of underneath eye wrinkles
Yinfeng JIANG ; Wenjuan ZHANG ; Leilei ZHI ; Weiguang HUA ; Qin YU ; Laiji MA ; Nan LU
Chinese Journal of Medical Aesthetics and Cosmetology 2017;23(1):43-46
Objective To investigate the applicability of clinical grade and a non-contact measurement method in evaluation of underneath eye wrinkles and to compare two methods.Methods A lot of 46 healthy Chinese women were recruited for this study.Underneath eye wrinkles severity was evaluated using clinical grade and a non-contact measurement method.The correlations were calculated for clinical grade and non-contact measurement parameters and age.The non-contact measurement parameters were classified by factor analysis.The correlations between age,clinical grade and factors were analyzed.Results The correlation coefficient between clinical grade in comparison to subject's age was 0.818.The parameters getting from non contact measurement were obviously correlated with age and clinical grade except SEr and SEsc;the correlation coefficients between parameters and age were-0.601 to 0.605;the correlation cofficients between parameters and clinical grade were-0.630 to 0.570.The non-contact measurement parameters could be classified into two factors;one represented wrinkle depth and roughness;the other represented wrinkle width and counts.These two factors were also obviously correlated with age and clinical grade.Conclusions Clinical grade and non contact measurement methods are both applicable in evaluation of underneath eye wrinkles.The parameters getting from two methods are obviously correlated with each other.
4.Preparation and immunogenicity identification of artificial antigen for luteoloside.
Bo ZHANG ; Zhi-lai ZHAN ; Li-ping KANG ; Yuan YUAN ; Lu-qi HUANG ; Feng-wei DING ; Tie-gui NAN
China Journal of Chinese Materia Medica 2015;40(22):4359-4363
Lonicerae Japonicae Flos was one of the most widely used traditional Chinese medicine for its special biological activities. The content of luteoloside, one of its major compounds, was an important standard for the quantity control of Lonicerae Japonicae Flos. The major method used for the detection of luteoloside was instrumental analysis. Compared with the ELISA method, instrumental analysis was time-consuming, complex pretreatment and low-throughout. Thus, it was significantly important to develop an enzyme-linked immunosorbent assay (ELISA) for luteoloside analysis. Here, the conjugates of luteoloside-bovine (LG-BSA) and luteoloside-ovalbumin (LG-OVA) were produced as the immunogen and coating antigen by the carbodiimide ( CDI) method, respectively. The conjugation ratio of carrier protein and the hapten in the conjugate were determined by UV-Vis spectrophotometry (UV). LG-BSA conjugate was used to immunize Bal b/c mice to produce antiserum. The titer and specificity of antiserum were detected by ELISA. The conjugation ratio of hapten and carries protein were 3. 7: 1 (LG-BSA) and 1. 0: 1 (LG-OVA). The antiserum titer was higher than 2 000 with the linear range of 18.4-4 852.4 μg x L(-1), R2 = 0.988 4 and IC50 = 298.7 μg x L(-1). The result showed that the conjugate antigen LG-BSA was synthesized successfully and the mice can produce specific antiserum injected with artificial antigen.
Animals
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Antibodies
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analysis
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immunology
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Antigens
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chemistry
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immunology
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Drugs, Chinese Herbal
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analysis
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Enzyme-Linked Immunosorbent Assay
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methods
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Female
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Immunization
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Lonicera
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chemistry
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immunology
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Mice
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Mice, Inbred BALB C
5.The value of MR angiography in the diagnosis of deep vein thrombosis of the lower limbs:comparative study with DSA
Min FENG ; Shu-Zhi WANG ; Jian-Ping GU ; Jun SUN ; Gun-Nan MAO ; Ling-Quan LU ; Xin-Dao YIN ;
Chinese Journal of Radiology 2000;0(11):-
Objective To assess the clinical values of MR angiography(MRA)in the detection of deep vein thrombosis of the lower limbs.Methods Two-dimensional time of flight(2D TOF)MRA was performed in thirty patients who were suspected of having deep vein thrombosis in the lower limbs.The findings of MRA were compared to that of digital subtraction angiography(DSA).Results twenty-five cases showed deep vein thrombosis in the lower limbs,the MRA findings included venous filling defect (14 cases),occlusions and interruptions of veins(8 cases),venous recanalizations(3 cases),collateral veins(25 cases).Taking the results of DSA as a golden standard,MRA detected all of the affected cases with only one case as the false positive.Conclusion 2D TOF MRA is a method of choice in the diagnosis of deep vein thrombosis of the lower limbs.
6.The comparison between the mechanisms of sodium selenite induced apoptosis and arsenic trioxide induced apoptosis in human acute promyelocytic leukemia cell line NB4 cells.
Lu ZUO ; Jian LI ; Ti SHEN ; Zhi-Nan ZHANG
Journal of Experimental Hematology 2002;10(3):195-200
In order to explore the differences between the mechanisms of selenite-induced apoptosis and arsenic induced apoptosis in NB4 cells, growth inhibition was determined by MTT test, apoptosis determined by DNA electrophoresis and analysis of intracellular DNA contents, reactive oxygen species and reduced glutathione in the cell were measured by Lucigenin dependent chemoluminescent (CL) test and spectrophotometry, and mitochondrial transmembrane potential was measured by flow cytometry. The results showed that: 5 micro mol/L sodium selenite similar to 1 micro mol/L arsenic trioxide could induce the apoptosis of NB4 cells after treatment for 24 hours. Both could elevate the level of reactive oxygen species and intensify mitochondrial transmembrane potential collapse, accompanied with decrease of reduced glutathione centent. The effect of selenium selenite on these aspects was more significant than those of arsenic trioxide. Elevation of intracellular glutathione in N-acytlcysteine pretreated NB4 cells could enhance the selenite induced apoptosis and oxidative stress, but ameliorate the arsenic trioxide induced apoptosis and oxidative stress. It was concluded that sodium selenite and arsenic trioxide can induce the apoptosis of NB4 cells, but there are significant differences between the mechanisms of selenite-induced and arsenic-induced apoptosis in NB4 cells, particularly in the influence of intracellular glutathione content on the drug action.
Acetylcysteine
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pharmacology
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Apoptosis
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drug effects
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genetics
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Arsenicals
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pharmacology
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Cell Division
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drug effects
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DNA, Neoplasm
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drug effects
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genetics
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metabolism
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Flow Cytometry
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Glutathione
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drug effects
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metabolism
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Humans
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Intracellular Membranes
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drug effects
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physiology
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Leukemia, Promyelocytic, Acute
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metabolism
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pathology
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Membrane Potentials
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drug effects
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Mitochondria
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drug effects
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physiology
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Oxides
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pharmacology
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Reactive Oxygen Species
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metabolism
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Sodium Selenite
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pharmacology
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Tumor Cells, Cultured
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drug effects
7.Inhibition of the activation of transcriptional factor NFkappaB during sodium selenite-induced apoptosis in NB4 cells.
Jian LI ; Lu ZUO ; Ti SHEN ; Zhi-Nan ZHANG
Journal of Experimental Hematology 2002;10(5):409-412
In order to evaluate the effect of sodium selenite on the activation of NFkappaB during selenite-induced apoptosis in NB4 cells, Western blot was used to measure the level of P65 in nuclear extraction of NB4 cells treated with sodium selenite to reflect the activation of NFkappaB; the apoptosis of NB4 cells was determined by morphological observation, DNA ladder electrophoresis and flow cytometry; and MTT test was used to measure the growth inhibition of cells. Results showed that sodium selenite (>/=5 micro mol/L) suppressed the cell growth, induced apoptosis and inhibited the activation of NF kappaB in a concentration- and time-dependency pattern. It was concluded that inhibition of NF kappaB might be one of the mechanisms in selenite-induced apoptosis in NB4 cells.
Apoptosis
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drug effects
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Cell Division
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drug effects
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Dose-Response Relationship, Drug
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Enzyme Activation
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drug effects
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Humans
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NF-kappa B
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antagonists & inhibitors
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metabolism
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Sodium Selenite
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pharmacology
8.Normal reference values and predict equations of heart function.
Zhi-nan LU ; Sun XING-GUO ; Song-shou MAO ; M J BUDOFF ; W W STRINGER ; Wan-gang GE ; Hao LI ; Jie HUANG ; Fang LIU ; Sheng-shou HU
Chinese Journal of Applied Physiology 2015;31(4):332-336
OBJECTIVEFor heart functional parameters, we commonly used normal range. The reference values and predict formulas of heart functional parameters and their relationships with individual characteristics are still lack.
METHODSLeft ventricular (LV) volumes (end-diastolic volume and end-systolic volume), stroke volume (SV), ejection fraction (EF) and cardiac output (CO) were measured by cardiac CT angiography (CAT) in 1 200 healthy Caucasian volunteers, men 807 and women 393, and age 20-90yr. The results are analyzed by high-accuracy three-dimensional imaging technology, and then measured the dynamic changes of the volumes of each atriam and ventricule during their contractions and relaxations. The gender, age, height and weight were analyzed by multiple linear regression to predict LV functional parameters.
RESULTSExcept the LVEF was lower in man than in women (P < 0.001), all other LV functional parameters of EDV, ESV, SV, FE and CO were higher in man (P < 0.001). Multiple linear regression indicated that age, gender, height and weight are all independent factors of EDV, ESV and SV (P < 0.001). CO could be significantly predicted by age, gender and weight (P < 0.001), but not height (P > 0.05). The predict equation for CO (L x min(-1)) = 6.963+0.446 (Male) -0.037 x age (yr) +0.013 x weight (kg).
CONCLUSIONAge, gender, height and weight are predictors of heart functions. The reference values and predict equations are important for noninvasive and accurate evaluation of cardiovascular disease and individualized treatment.
Adult ; Age Factors ; Aged ; Aged, 80 and over ; Body Height ; Body Weight ; Cardiac Output ; Female ; Heart ; physiology ; Humans ; Male ; Middle Aged ; Reference Values ; Sex Factors ; Stroke Volume ; Ventricular Function, Left ; Young Adult
9.Correlation analysis between WNK4 gene and essential hypertension.
Zhi-jun SUN ; Xin-nan WANG ; Jing-yu LU ; Qian DING ; Ling-yue DONG ; Yan-yan ZHAO
Acta Academiae Medicinae Sinicae 2003;25(2):145-148
OBJECTIVETo investigate association of mutation in WNK4 gene with essential hypertension and to analyze the expression of WNK4 gene.
METHODScSNP in WNK4 gene in a small samples was detected by sequencing, then PCR-RFLP was performed in 98 patients with essential hypertension and 95 control subjects. The expression profile of WNK4 gene was tested by RT-PCR.
RESULTSA cSNP was detected in WNK4 gene exon7 G1662A, and there were significant differences in the distribution of allele frequency of G1662A between essential hypertension group and control group. WNK4 gene were expressed in the tissues of kidney, brain, lung, heart, spleen and intestine of fetus.
CONCLUSIONWNK4 gene is well correlated with essential hypertension.
Adult ; Brain ; metabolism ; Female ; Gene Expression ; Gene Frequency ; Genes ; Genetic Predisposition to Disease ; Humans ; Hypertension ; genetics ; Kidney ; metabolism ; Lysine ; genetics ; Male ; Middle Aged ; Mutation ; Phenotype ; Protein-Serine-Threonine Kinases ; biosynthesis ; genetics ; RNA, Messenger ; biosynthesis ; genetics
10.Effect of Banxia Qinlian Decoction on Th17/IL-17 Immune Inflammatory Way of Sjögren's Syndrome NOD Model Mice.
Yan LU ; Yi CHEN ; Ya-nan WANG ; Hui LIU ; Ji-sheng ZHANG ; Wei-guo MA ; Zhi-ming SHEN ; Jie WANG ; Kang WANG ; Feng-xian MENG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(5):612-617
OBJECTIVETo explore the molecular mechanism of exocrine immune inflammatory injury of Sjögren's Syndrome and the intervention of Banxia Qinlian Decoction (BQD).
METHODSTotally 18 female NOD mice were randomly divided into the model group, the positive drug group, and the BQD group, 6 in each group. Six female BALB/c mice were recruited as a blank control group. Mice in the blank control group and the model group were gavaged with deionized water at the daily dose of 0.1 mL/10 g body weight. Tripterygium Tablet was administered by gastrogavage to mice in the positive group at the daily dose of 10 mg/kg. BQD was administered by gastrogavage to mice in the BQD group at the daily dose of 60 g crude drugs/kg. After 12 weeks of medication, mice were sacrificed. Their eyeballs were excised and blood collected. Tissues of bilateral parotids and submandibular glands were kept. mRNA transcriptional levels of IL-17, IL-6, type 3 muscarinic acetylcholine receptors (M3R), aquaporin protein-5 (AQP5) were detected by RT-PCR. Expression levels of M3R and AQP5 protein were detected by Western blot. Protein expression levels of IL-17 and IL-6 were detected by ELISA.
RESULTSCompared with the normal group, mRNA transcriptional levels and protein expression levels of IL-17, IL-6, M3R, and AQP5 were significantly up-regulated in the model group (P < 0.01). Compared with the model group, mRNA transcriptional levels and protein expression levels of IL-17, IL-6, M3R, and AQP5 were significantly down-regulated in the positive drug group and the BQD group with statistical difference (P < 0.01, P < 0.05). Compared with the BQD group, mRNA-transcriptional levels of IL-17, IL-6, and M3R, as well as M3R and AQP5 protein expression levels were significantly down-regulated in the positive drug group (all P < 0.01).
CONCLUSIONThe molecular mechanism of BQD in inhibiting SS exocrine neurotoxic injury might be possibly related to regulating Th17/IL-17 immune inflammatory way.
Animals ; Aquaporin 5 ; metabolism ; Disease Models, Animal ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Female ; Interleukin-17 ; metabolism ; Interleukin-6 ; metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Inbred NOD ; Sjogren's Syndrome ; drug therapy ; immunology ; Submandibular Gland ; Th17 Cells ; Up-Regulation