Laboratory animal science has become a main support for the development of life science and has occupied an importance place in higher medical education.The paper analyzed the characteristics and problems of laboratory animal science and proposed the ideas of applying conformational memory teaching in laboratory animal science teaching from there aspects:teaching method,teaching frame and teaching effect.These measures established the foundation for the cultivation of applied talents with comprehensive competence.

Acquired Immunodeficiency Syndrome ; blood ; complications ; diagnosis ; Antibodies, Viral ; blood ; Child ; Cough ; complications ; Fatal Outcome ; Fever ; complications ; HIV-1 ; immunology ; Humans ; Male

In recent years,nano-silver base inorganic antibacterial agents have been widely used in medicinal and biological fields.The recent research progress on nano-silver base inorganic antibacterial agents used in denture resin base,antibacterial effects,biocompatibility and the influence of the agents on the mechanical properties and color of the resin are introduced.

At present, the research regarding repair of spinal cord mainly focuses on tissue engineering. Neural tissue engineering materials provide three-dimensional template for tissue regeneration and also environment for synthesis of extracellular matrix. This paper summarizes the types of nerve transplant materials and the research progress in application for treatment of spinal cord injury, so as to provide theoretical evidence for repair of spinal cord injury. But some problems exist in application of nerve cell scaffold materials for repair of spinal cord injury: poor mechanical properties lead to slow degradation speed, causing difficulties in tissue reconstruction with respect to velocity and in subsequent reconstruction of porous three-dimensional scaffold. In recent years, novel biomaterials with specific repair function have been made by the engineering method through combining the biological molecule with specific signal identification function and available materials, which is an advanced projeot in the current field of biomaterials.

Objective To identify the inclusion compound of ginsenoside Rh2 with hydroxylpropyl-?-cyclodextrin (HP-?-CD) in aqueous solution, and to investigate the change of thermodynamic parameters during the inclusion process. Methods The measurements of the inclusion mechanism, inclusion molar ratio of the host and guest molecules, and change of thermodynamic parameters were carried out by the following methods separately; thin layer chromatography (TLC), differential scanning calorimetry (OSC), infrared (IR) spectrometry, phase solubility method, and thermodynamic method, respectively. Results That all the phase solubility diagrams showed a typical AL-type in various temperatures and suggested the formation of a soluble complex of 1∶1 molar ratio. TLC and IR Spectroscopy confirmed that the significant interaction between the host and guest molecules was probably due to the inclusion of aglycone of Rh2 molecule into the hydrophobic cavity of HP-?-CD molecule. The change in the thermodynamic parameters suggested that the inclusion could proceed spontaneously (?G

Objective To obtain the dynamic characteristics of the stretcher bed without absorber, and to optimize the absorber. Methods Pro/E and ADAMS were used to build the dynamic model of the stretcher bed on ambulance. The principle of random sine wave superposition and Visual Basic language were used to produce time-domain pavement roughness which could stimulate the model. The system was analyzed. Results The curve of vertical acceleration in time and frequency domain and the RMS of vertical acceleration without absorber were obtained by simulation., and a reasonably suited pair of stiffness and damping coefficient was found to reduce the vibration intensity. Conclusion The inherent frequency of the stretcher bed is 2.7Hz, which is out of the sympathetic vibration area of decubital body. Through adjusting and optimizing the stiffness and damping of vibration reduction equipment, the stretcher bed is more comfortable.

Based on the advantage of artificial lung, the background, principle and materials of membrane oxygenator are descried briefly. Besides, the purpose of ECC, ECMO, IVOX and TAL, advantage and disadvantage, newest re-search progress of the oxygenator are introduced. The aspects of IVOX and TAL can be modified, and the significance of TAL as to be the real artificial lung is pointed out. By anticipating the development trend of the oxygenator, the feature of ideal artificial lung and the study in the future can be summarized.

Objective This article was aimed to take exploratory evaluation on the scientific research performance of provincial key disciplines in a medical university,and to provide a scientific basis for the discipline evaluation related topics.Methods This paper took 29 provincial key disciplines in a medical university as the evaluation system.We calculated the scientific research performance of key disciplines.Data envelopment analysis (DEA) method was used to evaluate the scientific level of performance,while the use of TOPSIS and gray correlation analysis method to rank the efficiency of its research,and the results obtained by the three methods was used for statistical correlation analysis.Results According to the results calculated by DEA Method,there were 18 DEA efficient units and 11 non-DEA efficient units.While in this 11 non-DEA efficient units,8 of them needed to reduce re source inputs and the other 3 units needed to increase research outputs.Meanwhile there were significant positive correlations between evaluation results of TOPSIS Method andGrey Correlation Analysis Method with Data Envelopment Analysis Method,respectively [the correlation coefficient:rs 1 =0.797(P =0.000); rs 2=0.583(P =0.007); rs 3-0.536(P =0.003)].Conclusions Compared to TOPSIS Method and Grey Correlation Analysis Method,DEA Method not only could evaluate the ef fectiveness of discipline,but also provide evidence to improve the non-DEA efficient units.

Biomarkers ; blood ; Child ; Child, Preschool ; Critical Illness ; Early Diagnosis ; Enzyme-Linked Immunosorbent Assay ; Female ; Gastrointestinal Diseases ; blood ; diagnosis ; etiology ; Humans ; Infant ; Male ; Peptides ; blood ; Severity of Illness Index ; Trefoil Factor-2

Background There are two types of different questionnaires in dry eye diagnosis.But the associations about two questionnaires or questionnaire and clinical examination are still unclear.To effectively quantize the symptoms is helpful for a correct diagnosis of dry eye disease.Objective This survey was to evaluate the Standard Patient Evaluation of Eye Dryness (SPEED) and Ocular Surface Disease Index(OSDI) questionnaire for the diagnosis of dry eye and investigate the correlation between the clinical examinations and questionnaires. Methods A perspective cohort study was designed.Sixty-six patients were enrolled in this study.This study was approved by the Ethic Committee of Peking University First Hospital,and written informed consent was obtained from each subject before any ocular examination.SPEED-based and OSDI-based questionnaires were used to score the dry eye symptom and grouped according to severity of complains.Corneal fluorescence staining,tear film breakup time(BUT),Schirmer I test and tear film interferometry were performed in all patients.The correlations between two questionnaires scores and their association with clinical examinations were evaluated.Results The negative correlations were found between the SPEED-based score or OSDI-based score with BUT value(r=0.390,P=0.001 ;r=-0.395,P=0.001 ),but no significant correlations were seen between the SPEED-based score or OSDI-based score with Schirmer test( r=-0.081,P=0.515; r=-0.080,P=0.525)and tear film interferometry score(r=0.158,P=0.204;r=0.219,P=0.077).The BUT was significantly prolonged in mild symptom group compared with serious group(t=2.339,P=0.022),but no significant difference was seen in Schirmer Ⅰ test and tear film interferometry scores using SPEED-based questionnaire ( t =0.404,P =0.687 ; t =- 0.947,P =0.347 ) ; while the positive fluorescence staining rate between two groups was significantly different (x2 =0.164,P =0.685 ).When using OSDI-based questionnaire,significant difference in BUT was seen among mild,moderate and serious symptom groups ( F =11.871,P =0.000 ),and BUT in mild symptom group was delayed in comparison with moderat and serious groups( P=0.000,0.000).No significant differences were found in Schirmer Ⅰ test,tear film interferometry scores and fluorescence staining rate among three groups(F=1.432,P =0.246; F =2.799,P =0.068; x2 =6.026,P =0.050).SPEED score showed a positive correlation with OSDI score ( r =0.697,P =0.000 ). Conclusions Both OSDI and SPEED are effective tools for the evaluation of symptoms of dry eye.The two types of questionnaires are consistent in symptoms evaluation.