Along with the fast development of medical technology,the theory of holistic medical care is infiltrated into every aspect of clinical medical practices.In this paper,though the treatment of surgical hepatobiliary diseases under the direction of holistic medical care ,we emphasize the significance of holistic medical care in diagnosis and treatment ,recommend and canonize the usage of holistic medical care into the clinical practices.

【Objective】To investigate the relationship of plasma renin activity(PRA),angiotensin Ⅱ(Ang Ⅱ) and aldosterone(ALD) activities with syndrome patterns in patients with ventricular remodeling following myocardial infarction,and to supply neuroendocrine evidence for syndrome differentiation of ventricular remodeling following myocardial infarction.【Methods】Sixty patients with ventricular remodeling following myocardial infarction were classified as Qi stagnation and blood stasis(pattern 1,n=16),phlegm-turbidity blockage(pattern 2,n=15),yangQi deficiency(pattern 3,n=16) and yin-blood deficiency(pattern 4,n=13).Radioimmunoassay(RIA) was used to measure plasma renin,Ang Ⅱ and ALD levels in acute myocardial infarction patients 7 days after onset,and cardiac color ultrasound was used to examine left ventricular function.【Results】In patients with ventricular remodeling following myocardial infarction,left ventricular end diastolic diameter(LVEDD),left ventricular end diastolic volume(LVEDV),left ventricular end systolic volume(LVESV),and left ventricular ejection fraction(EF) increased as compared with those in the healthy.LVEDD,LVESV and LVEDV became larger in patients with pattern 3 and pattern 1 than those in patients with pattern 2 and pattern 4(P

OBJECTIVE:To study inhibitory effects of propofol on PC12 cells injury induced by glutamic acid via mitogen-acti-vated protein kinase/extra-cellular regulated kinase (MAPK/ERK) signal pathway. METHODS:PC12 cells were randomized into normal control group,model group(10 mmol/L glutamic acid),propofol low-concentrations,medium-concentrations and high-con-centrations groups(12.5,25,50 μmol/L+10 mmol/L glutamic acid). The optical density of cells,cell apoptosis,the phosphoryla-tion of ERK1/2 and the expression of c-fos,Bax,Bcl-2 were detected after treated with relevant medicine for 48 h. RESULTS:Compared with normal control group,optical density of cells,the phosphorylation of ERK1/2 and Bcl-2 decreased in model group (P<0.01);apoptotic rate,the expression of c-fos and Bax increased (P<0.01). Compared with model group,optical density of cells,the expression of Bcl-2 and the phosphorylation of ERK1/2 increased in propofol group (P<0.01);apoptosis rate,the ex-pression of c-fos and Bax decreased (P<0.05 or P<0.01). There were statistical significant between the different concentrations (P<0.01). CONCLUSIONS:Propofol can inhibit the apoptosis of PC12 cells induced by glutamic acid,which is associated with the up-regulation of ERK1/2 phosphorylation.

ObjectiveTo explore the effect of personality and psychosocial factors on irritable bowel syndrome (IBS).MethodsPersonality and psychological status of 46 IBS patients and 32 healthy individuals were evaluated with Eysenck Personality Questionaire (EPQ), Symptom Checklist-90 (SCL-90) and Life Events Scale (LES).ResultsIn EPQ, E (extroversion-introversion) scores of IBS group were lower than those of control group; N (neuroticism) scores of IBS group were significantly higher than those of control group (P<0.01). In SCL-90, the scores of somatization, compulsion, anxiety, depression and etc. were higher than those of control group (P<0.05). The frequency of meeting negative life events, the quantity of stimulus of meeting negative life events and the total quantity of stimulus of meeting life events of IBS patients within one year until episode were significantly higher than those of control group (P<0.01).ConclusionPersonality psychological characteristics and life events before illness are associated with the morbidity of IBS.

Objective To investigate the level of serum citrulline and its significance in the patients subject to intestinal transplantation. Methods Phenylisothiocyanate served as derivative reagent and the serum citrulline was determined by HPLC. Thirty-three healthy adults served as controls. Serum citrulline was measured in 2 cases of intestinal transplantation during different transplant periods. Results The serum citrulline level in 33 healthy adults was (16.87?5.97)?mol/L. Before operation, it was low (less than 10 ?mol/L) in the patients and after operation it reached to high levels and kept at higher levels. The maximium of serum citrulline was more than 30 ?mol/L in patient A and was over 50 ?mol/L in patient B. Following rejection, the serum citrulline level in patient A was decreased rapidly and simialr to that before operation and in patient B it was lower than that before operation. Conclusion The level of serum citrulline in intestinal transplantation patients was correlated with transplant rejection. More patients are reqiured for the further study.

Humans ; Infant, Newborn ; Solanaceous Alkaloids ; poisoning

The invasive model of gastric carcinoma in nude mice was constructed with gastric carcinoma cells SGC-7901 and SGC-7901-T2. The expression of type IVcollagenase (MT-MMP) and the changes of basement membrane (BM) in normal kidey tissue and malignant lesion of gastric were studied by immunohistochemical technique. The results showed that the positive rate of type IV collagenase(MT-MMP)was 36.6%in normal kidey tissue and 70% in malignant lesions (P

The disequilibrium of histone acetylation and deacetylation may cause tumor.Histone deacetylases (HDACs) maintain the equilibrium between histone acetylation and deacetylation by catalyzing the deacetylation of histone.They are related to many regulation processes containing transcription of oncogene,cell proliferation and differentiation,apoptosis and so on.HDACs inhibitors have become the hot field of researches,more than ten different HDACs inhibitors are testing for the treatment of both hematological and solid malignancies and show obvious anti-tumor activity.

Objective: Lactulose and mannitol excretion ratio in urine appears to be a marker to measure the intestinal permeability. Methods: A method has been developed for the simultaneous determination of lactulose and mannitol in urine by high performance liquid chromatography.After ultrafiltered and deionized,urine samples were analyzed with a lichrosher NH 2 column(4.6 mm?250 mm,i.d.,5 ?m),a mix of acetonitril water (72∶28,volume ratio) as mobile phase and refractive index detector. Results: The recoveries were 97.86%、 99.19% respectively and the relative standard deviation were 2.12、2.04.In 18 urine samples from healthy volunteers and patients who ingested the test solution containing 10 g lactulose and 5 g mannitol,the 6 h urinary excretion ratio of lactulose/mannitol was 0.0275?0.0113. Conclusions: It is suggested that lactulose and mannitol excretion ratio was anaccurate marker of intestinal permeability.

Objective To evaluate the clinical efficacy of ureteroscopy for the diagnosis and treatment of renal colic. Methods A total of 50 cases of renal colic were diagnosed and treated by ureteroscopy. Stones with size smaller than 0 5 cm were removed, but lithoclasty was performed on those stones with size bigger than 0 5 cm. In addition, J shaped catheters or ureteral catheters were indwelt for drainage. Results Successful removal of stones and lithoclasty were achieved in 12 and 30 cases, respectively. No urolithiasis and other lesions were found in 3 cases. Improvement was found in those cases with indwelt catheters. The average stay was 4-5 d. Correlative etiological factors were found in 2 patients by ureteroscopy. Conclusion Transurethral ureteroscopy is an effective and safe method for the diagnosis and treatment of renal colic.