Objective To explore the changes of GRO-α and VEGF in the peritoneal fluid of women with endometriosis and their correlation. Methods The levels of GRO-α and VEGF were detected by enzyme linked immunosorbent assay ( ELISA ) method in 39 cases of endometriosis and 25 normal cases of without endometriosis. Results The levels of GRO-αand VEGF in the peritoneal fluid of EMs group were ( 108.77 ± 20. 58 ) pg/ml and ( 199. 67 ± 99. 26 ) pg/ml separately, and it was significantly higher than those in control group ( 71.07 ± 18. 96 ) pg/ml, ( 115.46 ± 32. 39 ) pg/ml. The differences were statically significant( P <0. 05). The level of GRO-α in the peritoneal fluid of the patients in stage Ⅰ and Ⅱ was sig-nificantly lower than that in stage Ⅲ and Ⅳ[(99.43 ± 16.05)pg/ml vs (117.65 ± 20.8)pg/ml, P <0. 05] , while the level of VEGF in the peritoneal fluid of the patients in stage Ⅰ and Ⅱ was significantly higher than that in stage Ⅲ and Ⅳ[(246. 89 ± 114. 34) pg/ml vs ( 152. 82 ± 54. 55 ) pg/ml, P < 0. 05] .There was positive correlation between the level of GRO-α and r-AFS ( r = 0. 439, P < 0. 05 ), but no cor-relation between the level of VEGF and r-AFS ( r = -0. 210, P >0.05). There was no correlation be-tween peritoneal fluid levels of GRO-α and VEGF in the EMs group ( r =-0. 05, P>0. 05). Conclusion Compared with control group, the levels of GRO-α and VEGF in peritoneal fluid of women with endome-triosis were significantly increased, which indicated that the increased GR0-α and VEGF secretion involved in the pathogenesis of this disease. There was correlation between the peritoneal fluid level of GRO-α and the degree of EMs.

Tumor recurrence and drug resistance impact patients'therapeutic effect and prognosis seriously.In recent years,it has been found that epigenetic changes are involved in drug resistance.DNA methylation is one of the ways of epigenetic.DNA methylation of drug metabolism enzyme gene can affect gene's expression,leading to drug resistance.Methotrexate(MTX),a folic acid antagonist,is the most widely used drug in the treatment of malignant tumors,such as leukemia,osteosarcoma,head and neck cancer,and so on.Reduced folate carrier(RFC) is the main transporter cartier of MTX,and gene methylation is related to tumor cells'resistance to MTX.This paper reviews the role of RFC,relation of RFC methylation and methotrexate resistance and the factors of RFC methylation.

objective To evaluate the clinical implications of perioperative serum albumin (ALB),pre-albumin(PA)and high-sensitivity C-reactive protein(hs-CRP)changes in surgical patients.Methods This study included 103 surgical cases undergoing major,moderate or minor surgery respectively.Perioperative serum ALB.PA and hs-CRP levels were measured.Results were compared with 56 normal controls. Results Pre-and postoperative ALB was(43.6±5.1)g/L and(35.8±5.3)g/L in major surgery patients compared with that of(45.0±3.7)g/L and(38.3±5.7)g/L in moderate or minor surgery patients,both were significantly lower than that of(46.7±2.9)g/L in control group(F=54.07,P＜0.05);Pre-and postoperative PA level was(246±68)mg/L in major surgery patients while that was (270±53)mg/L and(207±70)mg/L respectively in moderate or minor surgery group,that in both groups were lower than that(279±40)mg/L in control group(F=41.18,P＜0.05),Pre-and postoperative hsCRP level was(5.3±5.1)mg/L and(12.7±4.2)mg/L in major surgery patients compared with that of (4.5±3.6)mg/L and(11.1±4.3)mg/L in moderate or minor surgery patients,again both that in the two groups were much higher than that of(1.0±0.7)mg/L in control group(F=69.87,P＜0.05).Conclusions Combined detection of serum ALB,PA and hs-CRP levels is a measure of surgical trauma especially for these patients suffering from postoperative infections.

the GGT level.Increased GGT and TG may be risk factors for diabetes mellitus.

Objective To explore the conversion rate of iodate ions (IO3-) being reduced to iodide ions (I-) by ascorbic acid (C6HsO6,VC) in simulated human gastric juice,and to provide references for further safety evaluation of edible salt iodized with potassium iodate.Methods An ion chromatography method was developed to detect iodide ions in simulated gastric juice.The conversion rate of iodate ions being reduced to iodide ions was used as the index for scavenging rate of iodate ions.In simulated gastric juice in vitro and in 37 C water bath,the scavenging effects of VC on iodate ions were determined in simulated gastric juice with different VC concentrations and simulated gastric juice acidities,as well as for different reaction time.Ion chromatography column:Dionex IonPac AS19 (250 mm × 4.0 mm);eluent:KOH 30 mmol/L (online produced),isocratic eluting,flow rate 1.0 ml/min,injection volume 100 μl,and detected by a conductivity detector.Results Performance of the method:within the range of 0-5 000 μg/L,iodide ions concentration and the chromatographic peak area had a good linearity (correlation coefficient r =0.999 7),and the detection limit of iodide ions was 20 μg/L.For quantification of iodide ions in simulated gastric juice,the relative standard deviation (RSD) of repeated measure for 6 times was ＜ 2.0％,the standard addition recovery rate was 97.6％-102.4％,and the overall average recovery rate was 99.4％.In the simulated gastric juice with a pH of 1.4 containing 5 mg/L and ≥ 10 mg/L VC,the reaction time to achieve 100％ conversion rate of iodate ions being reduced to iodide ions was 5 min and 2 min,respectively.In the simulated gastric juice with a pH of 3 containing 10 mg/L ascorbic acid,the reaction time to achieve 100％ conversion rate was 15 min.VC quantitatively reduced iodate ions to iodide ions by the stoichiometric relationship between reactants of the reduction reaction equation,and every 100 μg VC quantitatively reduced 24.0μg of iodine in iodate to iodide ions.Conclusions In simulated gastric juice,the reaction of iodate ions being reduced to iodide ions by VC is a stoichiometric reaction with relatively fast reaction rate,the scavenging rate of iodate ions by VC within the concentration level in human gastric juice can reach 100％.The results prompt that the iodate ions from edible salt iodized with potassium iodate in daily diet are reduced to iodide ions mainly in the human stomach.

The expression of microRNAs (miRNAs) alters obviously in ovarian cancer.Through miRNA profile based on a microarray platform and further research on individual one,they are found to be closely related to pathogenesis,progression,recurrence,and drug resistance of ovarian cancer and have a good prospect in applying it in the early diagnosis,detection recurrence,prognosis and therapy of ovarian cancer.

AIM: To study the change of Gq protein-phosphatidyl inositol signaling in the brain of rats with acute respiratory distress syndrome(ARDS).METHODS: Forty male Wistar rats were randomly divided into oleic acid groups(OA groups of 30 min,60 min,90 min and 120 min exposure) and control group.The ARDS model in rats was reproduced by intravenous injection of oleic acid(0.2 mL/kg in 2 min).The mean arterial blood pressure(MABP),blood gas indexes,malondialdehyde(MDA),lactate dehydrogenase(LDH) and creatine kinase(CK) in plasma and brain tissues were measured.G?q/11 protein and phospholipase C in brain tissues were detected by Western blotting.RESULTS: Compared to control group,MABP and PaO2 in all OA groups obviously decreased(P

Objective To identify the role of resistin in insulin resistance(IR) by endoplasmic reticulum(ER) stress in human umbilical vein endothelial cells ( HUVECs) and in rats.Methods HUVECs were cultured in vitro and disposed by resistin (R) or tauro ursodesoxycholic acid (tudca).Expressions of GRP78, P-akt and P-eNOS were determined using Western blotting.Thoracic aortic rings were made and their dilation function exposed to different concentrations of insulin was detected.Changes of vascular morphology were observed by HE staining.Results Results of Western blotting showed that expression of GRP78 was remarkably increased,but P-akt and P-eNOS were markedly decreased in R group.However, there was no difference in expressions of GRP78, P-akt and P-eNOS between tudca group and control group.The insulin induced vasodilation was decreased in R group and there was no difference between tudca group and control.Using HE staining, the R group showed significant medial thickening and proliferation of smooth muscle.Conclusion Resistin can induce insulin resistance in vascular endothelium cells by ER stress.

Objective:To establish an HPLC-FD method for determining the content of Angelica sinensis polysaccharide (ASP1) to lay foundation for its pharmacokinetic study in rats. Methods: Purified ASP1 was labeled with FITC by the method of Belder and Granath to obtain ASP1-FITC. The tissue samples were treated with 30% trichloroacetic acid and 11% NaOH before injection. The samples were determined by HPLC-FD. A PL aquagel-OH MIXED column was used,and the mobile phase was phosphate buffer( dis-solve NaH2PO32. 34g , Na2HPO34. 33 g and NaCl 11. 70 g into 1000 ml water) with pH of 7. 0. The flow rate was 0. 5 ml·min-1. The excitation and emission wavelengths was set at 495 nm and 520 nm, respectively. Results:The linear calibration curve was within the concentration range of 0. 25-40. 00 μg·ml-1(r=0. 9996)with the lower limit of quantification of 0. 20μg·ml-1 in tissue sam-ples. The extraction recovery of ASP1 was determined at low, medium and high concentration with the recovery of 91. 98%-114. 20%. The intra and inter-day RSDs were lower than 8. 31% and 2. 94%, respectively. Conclusion:The method to determine the content of ASP1 in rats by HPLC-FD with pre-column derivatization has been esablished. It is simple, accurate and reliable, which can be suc-cessfully applied in the study of pharmacokinetics and tissue distribution of ASP1 in rats.

Objective To study the DNA damage and repair of normal lung interstitial cells and human lung adenocarcinoma cells exposed to cigarette smoke. Methods Cultured human embryo lung fibroblasts (HLF) and human lung adenocarcinoma A549 cells. Mainstream smoke was collected by using dimerhyl sulfoxide (DMSO) and phosphate buffer solution (PBS) as absorbents. MTT assay was used to test the cytotoxicity of the solutions of cigarette smoke, then selected the concentrations of the solutions with no obvious cytotoxicity to treat cells and detected DNA damage and repair by comet assay. Results As treated with original solutions or 1/2 dilution of DMSO cigarette smoke solutions only, the Viability of cells was below 80%, but it was beyond 80% when treated with PBS solutions. The results showed that a significant difference of DNA damage was seen between the treated groups and negative control groups (P0.05),but the DNA damage caused by DMSO solutions was worse than PBS solutions significantly (P