Objective The physiological and biochemical changes were studied during the Panax notoginseng′s fruit development.Methods Dynamic changes of fruits size,fresh and dry weight,moisture content and soluble sugar,starch and protein contents were investigated.Results The moisture content was increased first and then dropped to 73.37% at maturity.The fresh and dry weight,starch and protein contents of P.notoginseng′s fruits were increased with the fruit development.The soluble sugar content was raised first,then decreased,and increased slightly at the lateral stage of fruit development.Conclusion The P.notoginseng′s fruits are mature at 80 d after peak anthesis;the decrease of moisture content is companied with the gradual increasing of fruit fresh and dry weight during the fruit development.The storage and utilization of nutrients in P.notoginseng′s fruits are closely related with the fruit development.

The impact of the pandemic, caused by SARS-CoV-2, on the management of malignancies has posed several challenges. Gestational trophoblastic diseases, being considered highly curative, are categorized as emergent cases which need immediate intervention with a level I priority for chemotherapy for its malignant spectrum. These guidelines have been drawn up to assist in decision-making and the implementation of such are dependent on the capabilities of each institution combined with their local experience.

Objective To develop and verify a plaque method for detection of infectious titer of tick-borne encephalitis virus(TBEV)strain(PHKT strain for short)adapted to primary hamster kidney(PHK)cells.Methods PHK cells were infected with TBEV,a primary mouse brain adaption strain,and passed consecutively for 12 passages.The titer of PHKT was detected by plaque method(Monolayer BHK-21 cells were infected with PHKT of various passages at different dilution ratios,and the plaque number was calculated by neutral red staining)and challenge titration in mouse brain(Mice were challenged with PHKT of various passages at different dilution ratios through brain cavity,0.03 mL for each,observed continuously for 14 days,and calculated for the median lethal dose(LD50)by Reed-Muench method)respectively,and the correlation between the results of two methods was analyzed.The developed plaque method for the detection of TBEV titer was verified for specificity,repeatability and intermediate precision.Results The plaque titer of PHKT virus was up to8.9 lgPFU/mL;The correlation between the results of plaque method and mouse brain challenge titration method was good(r = 0.92);The specificity of plaque method for detecting infectious titer of PHKT virus was good,and the coefficients of variation(CVs)of repeatability and intermediate precision were both less than 5%.Conclusion A plaque method for detecting infectious titer of PHKT virus was developed,which may be used as an alternative method for challenge titration in mouse brain.

Myelodysplastic syndromes (MDS) is a highly heterogeneous group of clonal disease,which is derived from hematopoietic.stem cells.Anemia is the main manifestation of most MDS patients and transfusion is required in about 90 ％ patients.Long-term blood transfusions can lead to iron overload (IOL).IOL can damage the iron balance,and excess free iron can deposite in the heart,liver and other vital organs,resulting in structural damage and organ dysfunction,which can deteriorate the prognosis,accelerate the transformation into acute myeloid leukemia,and increase the mortality.Iron chelation can improve the prognosis of patients with IOL,and prolong the overall survival and leukemia-free survival.

At present,research of genes and signal transduction pathway for etiology and pathogenesy of diseases is a hot topic.STAT3 signal transduction plays an important role in regulating cell proliferation,differentiation,survival and apoptosis.As a point of convergence for numerous oneogenie signaling pathways,STAT3 is constitutively activated in diverse human cancer cells.Current study suggest that activation of STAT3 signal transduction is closely related to the currence,development and malignant biological behaviors of tumor.STAT3 has been identified as a promising target for cancer treatment.

Objective:To detect the expression and significance of Dicer in gastric cell lines and gastric cancer tissue.Methods:Western-Blot and reverse transcriptase polymerase chain reaction(RT-PCR) were used to detect the expression of Dicer in normal gastric mucosa cell line GES-1,human gastric cancer cell line SGC-7901,25 surgical removed gastric cancer tissue and paired para-tumor tissue.Results:1.The expression of Dicer in human gastric cancer cell line SGC-7901 was lower than that in normal gastric mucosa cell line GES-1(P

Objective:To investigate the expressions of SEDL(X-linked spondyloepiphyseal dysplasiatarda,SEDL)gene and its encoded protein sedlin in a variety of tissues in mice.Methods:Real-Time PCR and Western Blot were used to detect the expression levels of the SEDL mRNA and sedlin protein in 9 tissues in mice,respectively.And the expression levels of them in bone tissues in mice of 1,2 and 4-week-old were also measured in the same way.Results:①Expressions of SEDL gene and its corresponding sedlin protein were existed in 9 tissues detected in mice.The relative expression levels of SEDL mRNA and sedlin protein in tissues in mice were significantly different (P

In university research institutions,when in charge of several projects at the same time,the project leader will inevitably pay not enough attention to every project.In order to ensure the progress and quality of each research project,the study designs and builds project managercentered management mechanisms,redesigns the research and development structure from the system perspective,establishes the crossbar management model,and further analyzes the internal communication mechanism,the power-and-responsibility division mechanism,and oversight mechanism.

Alzheimer Disease ; therapy ; Amyloid beta-Peptides ; metabolism ; Animals ; Humans ; Medicine, Chinese Traditional

The CD28-B7 are members of immunoglobulin superfamily.When B7 is engated by the CD28 ligand, a costimulatory signal occurs and transfers to T cell and it is important in the activation, proliferation, anti-apoptosis and promoting cytokine secretion of T cell.The CD28-B7 family is associated with tumor and autoimmune disease.