Objective The physiological and biochemical changes were studied during the Panax notoginseng′s fruit development.Methods Dynamic changes of fruits size,fresh and dry weight,moisture content and soluble sugar,starch and protein contents were investigated.Results The moisture content was increased first and then dropped to 73.37% at maturity.The fresh and dry weight,starch and protein contents of P.notoginseng′s fruits were increased with the fruit development.The soluble sugar content was raised first,then decreased,and increased slightly at the lateral stage of fruit development.Conclusion The P.notoginseng′s fruits are mature at 80 d after peak anthesis;the decrease of moisture content is companied with the gradual increasing of fruit fresh and dry weight during the fruit development.The storage and utilization of nutrients in P.notoginseng′s fruits are closely related with the fruit development.

The impact of the pandemic, caused by SARS-CoV-2, on the management of malignancies has posed several challenges. Gestational trophoblastic diseases, being considered highly curative, are categorized as emergent cases which need immediate intervention with a level I priority for chemotherapy for its malignant spectrum. These guidelines have been drawn up to assist in decision-making and the implementation of such are dependent on the capabilities of each institution combined with their local experience.

Objective To develop and verify a plaque method for detection of infectious titer of tick-borne encephalitis virus(TBEV)strain(PHKT strain for short)adapted to primary hamster kidney(PHK)cells.Methods PHK cells were infected with TBEV,a primary mouse brain adaption strain,and passed consecutively for 12 passages.The titer of PHKT was detected by plaque method(Monolayer BHK-21 cells were infected with PHKT of various passages at different dilution ratios,and the plaque number was calculated by neutral red staining)and challenge titration in mouse brain(Mice were challenged with PHKT of various passages at different dilution ratios through brain cavity,0.03 mL for each,observed continuously for 14 days,and calculated for the median lethal dose(LD50)by Reed-Muench method)respectively,and the correlation between the results of two methods was analyzed.The developed plaque method for the detection of TBEV titer was verified for specificity,repeatability and intermediate precision.Results The plaque titer of PHKT virus was up to8.9 lgPFU/mL;The correlation between the results of plaque method and mouse brain challenge titration method was good(r = 0.92);The specificity of plaque method for detecting infectious titer of PHKT virus was good,and the coefficients of variation(CVs)of repeatability and intermediate precision were both less than 5%.Conclusion A plaque method for detecting infectious titer of PHKT virus was developed,which may be used as an alternative method for challenge titration in mouse brain.

Objective: To explore the safety of using diagnostic hysteroscopy in patients with clinical stage one endometrial cancer,whether diagnostic hysteroscopy can increases the risk of positive peritoneal cytology in such patients and affects their post-operative prognosis.Methods: Medical records of 52 patients with clinical stage one endometrial cancer diagnosed by pre-operative hysteroscopy in our hospital were reviewed in this retrospective study,informations about cytological test of abdominal peritoneal fluid,type of operation,post-operative surgical stage and follow-up were collected.Results: The mean interval between hysteroscopy and laparotomy was 36 days(5~82d),2/52(3.8%) present positive peritoneal cytology,the mean survival time was 81 months(9~130m),the 5-year survival rate was 91.5%.Conclusion: Diagnostic hysteroscopy had not increase the risk of positive peritoneal cytology,also had no adverse effect on prognosis in clinical stage one endometrial cancer patients.

Objective To analyze the clinical significance of ECG changes in postures in the judgment of pathological atrioventricular block.Methods 62 patients with primary atrioventricular block were diagnosed by ECG examination.According to the complication,there were 31 cases with complication and 31 cases without complication.The two groups were taken sitting and squatting (1 min squat exercise,immediately lying position) ECG examination,the ECG abnormalities of the two groups were compared.Results In the comorbidity group,among the 31 patients,sitting electrocardiogram P-R interval returned to normal in 9 cases,abnormal in 22 cases,the normal rate was 29.0％ (9/31).Among the 22 cases of abnormal patients,squatting electrocardiogram P-R interval returned to normal in 1 case,abnormal in 21 cases,the normal rate was 4.5％ (1/22).Sitting,squatting electrocardiogram PR interval total returned to normal rate was 32.3％ (10/31).In the no complications group,among the 31 patients,sitting electrocardiogram PR interval returned to normal in 21 cases,abnormal in 10 cases,the normal rate was 67.7％ (21/31).Among the 10 cases of abnormal patients,squatting electrocardiogram P-R interval returned to normnal in 4 cases,abnormal in 6 cases,the normal rate was 40.0％ (4/10).Sitting,squatting electrocardiogran P-R interval total returned to normal rate was 80.7％ (25/31).Conclusion ECG changes in postures can early effectively determine the pathological and functional atrioventricular block.

Objective To find the mechanism of traditional Chinese medicine Heshouwuyin(HSWY) in anti-aging in ovary of the aging model rats,so as to provide theoretical and experimental evidence to anti-aging in female sexual gland.Methods Thirty-six rats were divided into three groups at random: normal group,aging model group,prevent aging group.Rats the in prevent aging group were subdivided into four groups: HSWY low dosage group,HSWY medium dosage group,HSWY high dosage group and Heshouwan(HSWW) group.The sub-acutely aging model rats were induced by D-galactose,and rats in the prevent aging group were received intragastric administration of HSWY and HSWW with D-galactose intraperioneal injection.The gene expressions of p53,p19~(ARF),Rb and p16 were detected in the ovaries of each group with reverse transcription polymerase chain reaction,and the protein expressions of Rb and p16 in the ovaries with Western blotting.Results The model group rats were in the aging status with low spirits and withered and yellowed hair.The ovaries of the rats in the model group expressed aging change.The gene expressions of p53,p19~(ARF),Rb and p16 were up-regulated and the protein expressions of Rb and p16 were increased in the ovaries of aging rats.HSWY significantly suppressed the gene expressions of p53,p19~(ARF),Rb and p16(P

Objective:To evaluate the inhibitory effect of Small interferirng RNA(siRNA) on human papilloma vinus(HPV)16 E6 oncogenes in nasopharyngeal carcinoma(NPC) cell line HNE-1,and observe the effects of HPV16 E6 silencing on NPC cell growth.Methods:Four siRNA against the HPV16 E6 gene were designed and transfected into HNE-1 cell respectively using RNAi-Mate transfection reagent.MTT was used to investigate the cellular proliferation after the transfection.RT-PCR was used to detect the expression of HPV16 E6 and protein level of HPV16 E6 was measured by Immunohistochemical staining.Results:After the most effective siRNA was transfected into HNE-1cell,the result of MTT indicated that the cellular proliferation was restrained remarkably,and the highest inhibitory rate was 32%.Meanwhile,RT-PCR and immunohistochemical staining showed that there was a significant decrease in HPV16 E6 mRNA and protein level.Conclusion:These results demonstrated that siRNA against HPV16 E6 could effectively downregulate HPV16 E6 expression in HNE-1 cell line,and inhibit the cellular proliferation.RNAi has great potential in the treatment of nasopharyngeal carcinoma as a new gene therapy.

Objective:To investigate the turnover and influencing factors for amniotic membrane transplantation under conjunctiva. Methods:Amniotic membrane stained with DTAF was implanted under conjunctiva of 20 New Zealand white rabbits. After transplantation,we observed the clinical change. The tissue samples from grafted area were observed by HE staining and under laser confocal micro-scope to trace amniotic membrane on 3,5,8,12,16 W after surgery. Results:The amniotic membrane was intact until 3W after trans-plantation. The amniotic membrane was at the bottom of the conjunctiva and fascia,near the top of the sclera. At the 5th week after surgery,the amniotic membrane was bandshaped and began dissolved. Remnants of the amniotic membrane could be identified at the 8th week after surgery. After 12 W,the amniotic membrane basically dissolved,but remnants of curmbly erythroic tissue were locally left. The amniotic membrane totally dissolved after 16W. The types of inflammatory cells were mainly plasma cells,mononucleimacrophages and lymphocytes. At the 3th week after surgery,the inflammatory reaction reached the peak,then the inflammatory cells decreased. The main pathologic reaction was chronic inflammation.Laser confocal microscope discovered that the amniotic membrane persisted for 12 weeks under conjunctiva. Conclusion:The amniotic membrane implanted under conjunctiva persisted for 12 weeks and finally dissolved.

Objective:To detect the expression and significance of Dicer in gastric cell lines and gastric cancer tissue.Methods:Western-Blot and reverse transcriptase polymerase chain reaction(RT-PCR) were used to detect the expression of Dicer in normal gastric mucosa cell line GES-1,human gastric cancer cell line SGC-7901,25 surgical removed gastric cancer tissue and paired para-tumor tissue.Results:1.The expression of Dicer in human gastric cancer cell line SGC-7901 was lower than that in normal gastric mucosa cell line GES-1(P

Objective:To investigate the expressions of SEDL(X-linked spondyloepiphyseal dysplasiatarda,SEDL)gene and its encoded protein sedlin in a variety of tissues in mice.Methods:Real-Time PCR and Western Blot were used to detect the expression levels of the SEDL mRNA and sedlin protein in 9 tissues in mice,respectively.And the expression levels of them in bone tissues in mice of 1,2 and 4-week-old were also measured in the same way.Results:①Expressions of SEDL gene and its corresponding sedlin protein were existed in 9 tissues detected in mice.The relative expression levels of SEDL mRNA and sedlin protein in tissues in mice were significantly different (P