Objective To find the mechanism of traditional Chinese medicine Heshouwuyin(HSWY) in anti-aging in ovary of the aging model rats,so as to provide theoretical and experimental evidence to anti-aging in female sexual gland.Methods Thirty-six rats were divided into three groups at random: normal group,aging model group,prevent aging group.Rats the in prevent aging group were subdivided into four groups: HSWY low dosage group,HSWY medium dosage group,HSWY high dosage group and Heshouwan(HSWW) group.The sub-acutely aging model rats were induced by D-galactose,and rats in the prevent aging group were received intragastric administration of HSWY and HSWW with D-galactose intraperioneal injection.The gene expressions of p53,p19~(ARF),Rb and p16 were detected in the ovaries of each group with reverse transcription polymerase chain reaction,and the protein expressions of Rb and p16 in the ovaries with Western blotting.Results The model group rats were in the aging status with low spirits and withered and yellowed hair.The ovaries of the rats in the model group expressed aging change.The gene expressions of p53,p19~(ARF),Rb and p16 were up-regulated and the protein expressions of Rb and p16 were increased in the ovaries of aging rats.HSWY significantly suppressed the gene expressions of p53,p19~(ARF),Rb and p16(P

The development of effective therapy for children with acute lymphoblastic leukemia (ALL) is one of the greatest successes of clinical oncology. However, cure rates for adults with ALL remain relatively low. Adolescents are the special population between children and adults. These patients group will be received protocol from either pediatric or adult oncologists depending on referral pattern. In recent years, researchers from abroad large cooperative groups show pediatric-based protocol has better outcome than adult-based protocol. In this article, the protocol of ALL in children and adults are compared . It seems the first and probably the most important reason is the dose intensity of the nonmyelosuppressive chemotherapeutic agents, especially asparaginase.

Objective To explore the relationship between transitional care model and diabetic patients. Methods 112 patients with type 2 diabetes mellitus were divided into observation group and control group according to random number table. Control group used the doctor as the center patient management mode. The observation group used thepatient-centeredpatient management, application of home nursing service. Results Glycosylated hemoglobin (HbA1c), triglyceride (TG), high-density lipoprotein (HGL), 2 h postprandial blood glucose (2hPBG), fasting blood glucose (FBG), body mass index (BMI) of observation group before treatment respectively were (11.2±2.4)%, (3.7±1.3) mmol/L, (0.5±0.2) mmol/L, (13.6±2.4) mmol/L, (11.8±2.9) mmol/L, (25.9±4.9) kg/m2. Patients discharged from hospital after care for 3 months respectively were (6.8±0.9)%, (1.0±0.3) mmol/L, (2.5±0.4) mmol/L, (8.9±1.5) mmol/L, (6.8 ± 2.0) mmol/L, (20.8 ± 5.8) kg/m2. There was statistically significant difference (t=3.5-6.6,P<0.05). Control group before treatment respectively were (11.9 ± 3.6) %, (3.8 ± 1.5) mmol/L, (0.6 ± 0.3) mmol/L, (13.9 ± 2.9) mmol/L, (11.6 ± 3.2) mmol/L, (25.9 ± 6.8) kg/m2,while patients discharged from hospital for 3 months respectively were (10.9±3.4)%, (3.3±0.7) mmol/L, (0.7±0.2) mmol/L, (12.8±4.2) mmol/L, (10.6± 2.6) mmol/L, (25.1 ± 6.6) kg/m2, there was no statistically significant difference (t=0.05-1.36,P>0.05). Patients discharged from hospital after 3 months, perception, diet, medication, exercise adherence score of observation group was (34.98 ± 5.67), (41.98 ± 5.00), (40.29 ± 5.60), (40.45 ± 7.21) points, which were obviously higher than (21.18±4.75) , (28.46±4.26), (21.88±4.58), (20.98±2.69) points of control group, the difference was statistically significant (t=13.96-19.03,P<0.01). Conclusions Using home nursing service mode in type 2 diabetes care is helping to improve blood sugar levels and improve patient compliance after discharge, fully improve the patients′prognosis.

Objective: To investigate the expression of histone deacetylase 2 (HDAC2) in esophageal squamous cell carcinoma (ESCC), and to explore the effects of downregulation of HDAC2 expression on cell proliferation, cell cycle and apoptosis of EC9706 cells and their possible molecular mechanisms. Methods: The expression of HDAC2 protein in ESCC tissues was detected by immunohistochemistry. HDAC2 small interfering RNA (siRNA) and control siRNA were transfected into ESCC EC9706 cells, and then the EC9706 cells were randomly divided into three groups, including untreated group, siRNA control group and HDAC2 siRNA group. The expression of HDAC2 protein in EC9706 cells was detected by Western blotting. The cell proliferation status was examined by cell counting Kit-8 (CCK-8) method. The cell cycle and apoptosis were detected by flow cytometry (FCM). The expressions of proteins related to cell proliferation, cell cycle and apoptosis were detected by Western blotting. Results: The positive expression rate of HDAC2 in ESCC tissues (79.71%) was significantly higher than those in normal esophageal mucosa (51.11%) and paracancerous tissues (dysplasia, 23.19%), and there was a significant difference among these three tissues (χ2=44.121, P=0.000). The expression of HDAC2 protein wasnot associated with age and gender of patients with ESCC (both P>0.05), but closely associated withhistological grade, invasive depth, TNM stage and lymph node metastasis (all P<0.05). HDAC2 siRNA effectively down-regulated the expression of HDAC2 protein, obviously inhibited the cell proliferation, arrested cell cycle at G0/G1 phase and induced apoptosis of EC9706 cells. In addition, the result of Western blotting showed that down-regulation of HDAC2 expression could markedly increase the expressions of p21 protein and apoptosis-related bax protein while decrease the expressions of cyclin D1 and bcl-2 proteins. Conclusion: HDAC2 may play an important role in occurrence and development of ESCC. The downregulation of HDAC2 expression-mediated proliferation inhibition, cell cycle arrest and apoptosis of EC9706 cells may be tightly associated with the increased expression levels of p21 and baxas well as the decreased expression levels of cyclin D1 and bcl-2. Copyright© 2011 by TUMOR.

Objective To explore clinical value of detection of carcinoembryonic antigen(CEA) ,cytokeratin 19 fragment(CY-FRA21-1) and neuron specific enolization enzyme(NSE) in pleural effusion and serum during the differential diagnosis and patho-logical classification of lung cancer .Methods 180 patients with pleural effusion were divided into two groups ,one including 55 be-nign pleural effusion cases(benign pleural effusion group) and the other including 125 lung cancer cases(lung cancer group) .CEA , NSE and CYFRA21-1 in pleural effusion and serum of two groups were measured ,respectively ,and the measure results between two groups were conducted .Results CEA ,NSE and CYFRA21-1 levels in lung cancer group were significantly higher than those in benign pleural effusion group(P<0 .05) .CEA levels in pleural effusion and serum in lung adenocarcinoma group were significantly higher than those in benign pleural effusion group ,lung squamous carcinoma group and small cell lung cancer group(P<0 .05) .CY-FRA21-1 levels in pleural effusion and serum in lung squamous carcinoma group were significantly higher than those in benign pleu-ral effusion group ,lung adenocarcinoma group and small cell lung cancer group(P<0 .01) .NSE levels in pleural effusion and serum in small cell lung cancer group were significantly higher than those in benign pleural effusion group ,lung adenocarcinoma group and lung squamous carcinoma group(P< 0 .01) .The positive detection rate of CEA in lung adenocarcinoma group was significantly higher than that in lung squamous carcinoma group and small cell lung cancer group(P<0 .05) .The positive detection rate of CY-FRA21-1 in lung squamous carcinoma group was significantly higher than that in lung adenocarcinoma group and small cell lung cancer group(P<0 .05) .The positive detection rate of NSE in small cell lung cancer group was significantly higher than that in lung squamous carcinoma group and lung adenocarcinoma group(P<0 .01) .Conclusion CEA ,CYFRA21-1 and NSE in pleural effusion and serum are specific tumor markers of lung adenocarcinoma ,lung squamous carcinoma and small cell lung cancer ,respectively , which may have a certain clinical significance for the differential diagnosis and pathological classification of lung cancer .

Motivational interviewing was a consultation model which centered on patients, and changed patients′unhealthy behaviors by changing their motivations, and promoting their health. Motivational interviewing had been widely used in improving the capacities of patients′self-management with chronic diseases in abroad. Through reviewing the application in chronic disease self-management of motivational interviewing at home and abroad, the paper pointed out the prospect and problems that needed to be solved in our country and provided references for further research on motivational interviewing in self-management of chronic diseases.

Ginsenosides (GS)as the main ingredient of ginseng,include dozens of monomer,such as Rg3,Rh2,Rd,Rh1 ,Rg2,etc.GS and various monomers have different anti-hepatoma mechanisms.The main mechanism includes the following aspects,inducing the apoptosis of liver cancer cells,inhibiting the prolifera-tion,invasion and metastasis,reversing the multidrug resistance of liver cancer cells,increasing therapeutic effects by combining with other treatments,improving the immune function of organism and inhibiting hepatic glu-cose production.Different GS monomers play different roles of anti-hepatoma through their respective channels.

Objective To observe the application effect of intra-aortic balloon pump (IABP) in the emergency coronary intervention.Methods 76 cases patients with acute myocardial infarction complicated with cardiogenic shock were selected in the Third Affiliated Hospital of Qiqihar Medical University.41 cases in the observation group used IABP,35 cases in the control group did not use the IABP.The left ventricular ejection fraction (LVEF),ejection fraction improvement rate (postoperative EF/pre-operative EF),and the incidence of postoperative complications of IABP were compared between two groups.Results LVEF changes,the rate of improvement in ejection fraction,systolic and diastolic blood pressure were statistically significant; after six months,LVEF changes and the number of cases of death in patients were also statistically different between two groups.Conclusions For the patients with AMI and hemodynamic disorders undergoing PCI,timely IABP can effectively improve the patients’ left ventricular ejection fraction,improve the patients’ blood pressure levels and reduce mortality rate of patients.

Objective To evaluate the distribution characteristics of left ventricular systolic dyssynchrony (LV-SD) in dilated cardiomyopathy (DCM) patients with chronic heart failure (CHF) and normal QRS wave width, by pulsed-wave Doppler tissue imaging (PW-DTI), and study its relation with left ventricular systolic function, ventricular remodeling, and functional mitral regurgitation (FMR). Methods The time to peak systolic velocity (Ts) in 12 left ventricular segments was evaluated by PW-DTI, from which the standard deviation (SD) of Ts in the 12 segments (Ts-SD) and maximum Ts difference (Ts-maxD) were calculated. Results Ts-SD and Ts-maxD in the 12 LV segments of the DCM patients with CHF were significantly higher than those of the healthy controls (P＜0.01). In DCM patients with CHF and normal QRS wave width, the incidence of LV-SD was 29.8% (14/47) and the inferior wall was the most frequent distribution site of contraction delay. Linear regression analysis revealed a negative correlation between Ts-SD, Ts-maxD, and left ventricular ejection fraction (LVEF) (P＜0.01), but a positive correlation between Ts-SD, Ts-maxD and left ventricular end-diastolic volume (LVEDV), lefe ventricular end-systolic volume (LVESV), New York Heart Association (NYHA) cardiac function, FMR (P＜0.01) in DCM patients with CHF. Conclusion LV-SD exists in DCM patients with normal QRS width. LV-SD aggravates the LV systolic function damage, which is closely associated with left ventricular remodeling. LV-SD may contribute to the FMR in DCM patients.

OBJECTIVE:To explore the feature and regularity of ADR induced by animal bone polypeptide medicine,so as to provide reference for rational use of these drugs in clinical practice. METHODS:In retrospective analysis,96 cases of ADR oc-curred in our hospital during Jan. 2009-Jun. 2016 were analyzed statistically in terms of patients'gender and age,drugs involved, occurrence time,route of administration,organs and systems involved,association,prognosis,etc. RESULTS:Among 96 cases (including 17 serious ADR),the ratio of male to female was 1.34 : 1,and 56.25% of them were above 40 years old. ADR mainly occurred within 15 minutes after medication(39.58%)dominated by intravenous drip. Organs/systems involved in ADR were main-ly lesion of skin and its appendants(34.81%),followed by respiratory system damage(18.35%)and systemic damage(16.46%). In aspect of ADR association,41 were probable(42.71%)and 55 were possible(57.29%). After suffering from ADR,43 patients (44.79%)were cured after drug withdrawal or corresponding treatment,and 53 patients(55.21%)were recovered. CONCLU-SIONS:Medical staff should master the regularity and characteristics of ADR induced by animal bone polypeptide medicine,while strengthen rational drug use management and monitoring to ensure the safety and effectiveness of clinical drug use.