The development of effective therapy for children with acute lymphoblastic leukemia (ALL) is one of the greatest successes of clinical oncology. However, cure rates for adults with ALL remain relatively low. Adolescents are the special population between children and adults. These patients group will be received protocol from either pediatric or adult oncologists depending on referral pattern. In recent years, researchers from abroad large cooperative groups show pediatric-based protocol has better outcome than adult-based protocol. In this article, the protocol of ALL in children and adults are compared . It seems the first and probably the most important reason is the dose intensity of the nonmyelosuppressive chemotherapeutic agents, especially asparaginase.

Objective To find the mechanism of traditional Chinese medicine Heshouwuyin(HSWY) in anti-aging in ovary of the aging model rats,so as to provide theoretical and experimental evidence to anti-aging in female sexual gland.Methods Thirty-six rats were divided into three groups at random: normal group,aging model group,prevent aging group.Rats the in prevent aging group were subdivided into four groups: HSWY low dosage group,HSWY medium dosage group,HSWY high dosage group and Heshouwan(HSWW) group.The sub-acutely aging model rats were induced by D-galactose,and rats in the prevent aging group were received intragastric administration of HSWY and HSWW with D-galactose intraperioneal injection.The gene expressions of p53,p19~(ARF),Rb and p16 were detected in the ovaries of each group with reverse transcription polymerase chain reaction,and the protein expressions of Rb and p16 in the ovaries with Western blotting.Results The model group rats were in the aging status with low spirits and withered and yellowed hair.The ovaries of the rats in the model group expressed aging change.The gene expressions of p53,p19~(ARF),Rb and p16 were up-regulated and the protein expressions of Rb and p16 were increased in the ovaries of aging rats.HSWY significantly suppressed the gene expressions of p53,p19~(ARF),Rb and p16(P

Objective To observe the application effect of intra-aortic balloon pump (IABP) in the emergency coronary intervention.Methods 76 cases patients with acute myocardial infarction complicated with cardiogenic shock were selected in the Third Affiliated Hospital of Qiqihar Medical University.41 cases in the observation group used IABP,35 cases in the control group did not use the IABP.The left ventricular ejection fraction (LVEF),ejection fraction improvement rate (postoperative EF/pre-operative EF),and the incidence of postoperative complications of IABP were compared between two groups.Results LVEF changes,the rate of improvement in ejection fraction,systolic and diastolic blood pressure were statistically significant; after six months,LVEF changes and the number of cases of death in patients were also statistically different between two groups.Conclusions For the patients with AMI and hemodynamic disorders undergoing PCI,timely IABP can effectively improve the patients’ left ventricular ejection fraction,improve the patients’ blood pressure levels and reduce mortality rate of patients.

Ten compounds, including five steroidal saponins and five flavonol glycosides, were isolated from the whole plant of Solanum coagulans by means of column chromatographies over silica gel, ODS, Sephadex LH-20, and preparative HPLC. Based on analysis of MS and NMR spectroscopic data, their structures were established as anguiviosides XV (1), smilaxchinoside A (2), methylprotodioscin (3), protodioscin (4), solamargine (5), 3', 4', 5-trihydroxy-7-methoxy-6-C-β-D-glucopyranoside (6), brainoside B (7), camsibriside A (8), kampferol 3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (9), and quercetin-3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (10). All the compounds were first isolated from this plant. In the in vitro assays, compounds 4 and 5 showed cytotoxic activity against SMCC-7721 and NCI-H460.
Cell Line, Tumor ; Flavonols ; chemistry ; isolation & purification ; pharmacology ; Humans ; Saponins ; chemistry ; isolation & purification ; pharmacology ; Solanum ; chemistry

Objective To explore the changes of GRO-α and VEGF in the peritoneal fluid of women with endometriosis and their correlation. Methods The levels of GRO-α and VEGF were detected by enzyme linked immunosorbent assay ( ELISA ) method in 39 cases of endometriosis and 25 normal cases of without endometriosis. Results The levels of GRO-αand VEGF in the peritoneal fluid of EMs group were ( 108.77 ± 20. 58 ) pg/ml and ( 199. 67 ± 99. 26 ) pg/ml separately, and it was significantly higher than those in control group ( 71.07 ± 18. 96 ) pg/ml, ( 115.46 ± 32. 39 ) pg/ml. The differences were statically significant( P <0. 05). The level of GRO-α in the peritoneal fluid of the patients in stage Ⅰ and Ⅱ was sig-nificantly lower than that in stage Ⅲ and Ⅳ[(99.43 ± 16.05)pg/ml vs (117.65 ± 20.8)pg/ml, P <0. 05] , while the level of VEGF in the peritoneal fluid of the patients in stage Ⅰ and Ⅱ was significantly higher than that in stage Ⅲ and Ⅳ[(246. 89 ± 114. 34) pg/ml vs ( 152. 82 ± 54. 55 ) pg/ml, P < 0. 05] .There was positive correlation between the level of GRO-α and r-AFS ( r = 0. 439, P < 0. 05 ), but no cor-relation between the level of VEGF and r-AFS ( r = -0. 210, P >0.05). There was no correlation be-tween peritoneal fluid levels of GRO-α and VEGF in the EMs group ( r =-0. 05, P>0. 05). Conclusion Compared with control group, the levels of GRO-α and VEGF in peritoneal fluid of women with endome-triosis were significantly increased, which indicated that the increased GR0-α and VEGF secretion involved in the pathogenesis of this disease. There was correlation between the peritoneal fluid level of GRO-α and the degree of EMs.

One hundred and twenty four patients with cervical intra-epithelial neoplasia(CIN group)and 300 women in routine health check(control group)were recruited in the study.Ureaplasma urealyticum(UU)DNA in cervical secretions was detected by fluorescent quantitative PCR(FQ-PCR),and detectopm pf HPV was also performed in 103 CIN cases.The positive rate of UU was 64.5%in CIN group and 38.7%in control group(P＜0.05).The results demonstrated that UU infection may be associated with cervical intra-epithelial neoplasia,especially for high-grade lesions;and there may be interaction between UU and HPV infection in development of CIN.

Objective To evaluate the distribution characteristics of left ventricular systolic dyssynchrony (LV-SD) in dilated cardiomyopathy (DCM) patients with chronic heart failure (CHF) and normal QRS wave width, by pulsed-wave Doppler tissue imaging (PW-DTI), and study its relation with left ventricular systolic function, ventricular remodeling, and functional mitral regurgitation (FMR). Methods The time to peak systolic velocity (Ts) in 12 left ventricular segments was evaluated by PW-DTI, from which the standard deviation (SD) of Ts in the 12 segments (Ts-SD) and maximum Ts difference (Ts-maxD) were calculated. Results Ts-SD and Ts-maxD in the 12 LV segments of the DCM patients with CHF were significantly higher than those of the healthy controls (P＜0.01). In DCM patients with CHF and normal QRS wave width, the incidence of LV-SD was 29.8% (14/47) and the inferior wall was the most frequent distribution site of contraction delay. Linear regression analysis revealed a negative correlation between Ts-SD, Ts-maxD, and left ventricular ejection fraction (LVEF) (P＜0.01), but a positive correlation between Ts-SD, Ts-maxD and left ventricular end-diastolic volume (LVEDV), lefe ventricular end-systolic volume (LVESV), New York Heart Association (NYHA) cardiac function, FMR (P＜0.01) in DCM patients with CHF. Conclusion LV-SD exists in DCM patients with normal QRS width. LV-SD aggravates the LV systolic function damage, which is closely associated with left ventricular remodeling. LV-SD may contribute to the FMR in DCM patients.

Objective:To control the maintenance cost of medical equipment and improve the efficiency and service level for maintenance, and to research the management about classification maintenance for medical equipment based on analytic hierarchy process (AHP).Methods: 69 clinical department and 14 engineers were involved in the questionnaire survey and the evaluated factors were obtained from their questionnaires. The (AHP) was used to determine what is the factor weight of maintenance for the medical equipment, and then calculate the maintenance scores of the equipment. Finally, the weight scores were applied to ABC classification method and the equipments will be divided into three types (A\B\C) as this method, and they will be managed by different mode.Results: Depended on the sixth order analysis data from 6 factors of evaluation (price, frequency, stability, running time, standby factor and difficulty of maintenance) that were compared between any two factors by using AHP, through comprehensive scores ratio to got A\B\C classification and work out relative implement countermeasure of maintenance. The new classification management mode has achieved planning and preventive maintenance for key equipment, regular maintenance for major equipment, and maintenance after event for general equipment. After the method was applied, the fault rate of second half of 2016 year was decrease 30% compared with same time of 2015 year. The total cost of maintenance of 2016 year decreased 10% compared with that of 2015 year. And the satisfaction of clinical department for department of equipment was enhanced.Conclusion: The AHP is applied to classified manage medical equipment and maintain them as weight of factor. This method has resolved many problems on maintenance of equipment, such as confused primary and secondary, and formalism and so on. According to the factor weight of maintenance to manage medical equipment at classification mode, hospital can catch key point of maintenance of equipment, and the maintenance cost can be efficiently controlled, and the work efficiency and clinical service level of equipment department can be improved and enhanced.

Objective To investigate the clinical effect and analysis of Shah Glenn Dean combined with acarbose in the treatment of newly diagnosed elderly patients with type 2 diabetes mellitus.Methods 60 cases of newly diagnosed elderly patients treated in our hospital during the period from January 2015 to January 2017 in patients with type 2 diabetes mellitus is selected as the object of this study, they were randomly divided into control group and study group, 30 cases in each group.The control group was treated with acarbose treatment, the study group was treated with Shah Glenn Dean combined acarbose treatment, treatment time was 3months, glycosylated hemoglobin, after two groups of patients were in different treatment of hypoglycemia were observed and compared the rate of change, body mass index and blood glucose level changes.Results The patients in the control group, the incidence of hypoglycemia was 23.33%, patients in the study group, the incidence of hypoglycemia was 6.67%, patients in the study group,the incidence of hypoglycemia was significantly lower than that of control group, the difference was statistically significant between the groups(P <0.05); glycosylated hemoglobin levels in the study group were significantly lower than that of the control group of two patients, the difference was statistically significant between the groups(P <0.05); the total cost of the patients in the treatment group compared with the control group had no significant difference, the difference was not statistically significant.Conclusion Significant clinical therapeutic effect of saxagliptin combined with acarbose in the treatment of newly diagnosed elderly patients with type 2 diabetes, can make the patient's blood glucose levels were significantly lower,with high security, the incidence of hypoglycemia was significantly reduced, but also can reduce the weight of patients, the total cost in the course of treatment did not increase that will not cause economic burden on patients, it is worthy of clinical application.

Tumor recurrence and drug resistance impact patients'therapeutic effect and prognosis seriously.In recent years,it has been found that epigenetic changes are involved in drug resistance.DNA methylation is one of the ways of epigenetic.DNA methylation of drug metabolism enzyme gene can affect gene's expression,leading to drug resistance.Methotrexate(MTX),a folic acid antagonist,is the most widely used drug in the treatment of malignant tumors,such as leukemia,osteosarcoma,head and neck cancer,and so on.Reduced folate carrier(RFC) is the main transporter cartier of MTX,and gene methylation is related to tumor cells'resistance to MTX.This paper reviews the role of RFC,relation of RFC methylation and methotrexate resistance and the factors of RFC methylation.