Objective To find the mechanism of traditional Chinese medicine Heshouwuyin(HSWY) in anti-aging in ovary of the aging model rats,so as to provide theoretical and experimental evidence to anti-aging in female sexual gland.Methods Thirty-six rats were divided into three groups at random: normal group,aging model group,prevent aging group.Rats the in prevent aging group were subdivided into four groups: HSWY low dosage group,HSWY medium dosage group,HSWY high dosage group and Heshouwan(HSWW) group.The sub-acutely aging model rats were induced by D-galactose,and rats in the prevent aging group were received intragastric administration of HSWY and HSWW with D-galactose intraperioneal injection.The gene expressions of p53,p19~(ARF),Rb and p16 were detected in the ovaries of each group with reverse transcription polymerase chain reaction,and the protein expressions of Rb and p16 in the ovaries with Western blotting.Results The model group rats were in the aging status with low spirits and withered and yellowed hair.The ovaries of the rats in the model group expressed aging change.The gene expressions of p53,p19~(ARF),Rb and p16 were up-regulated and the protein expressions of Rb and p16 were increased in the ovaries of aging rats.HSWY significantly suppressed the gene expressions of p53,p19~(ARF),Rb and p16(P

The development of effective therapy for children with acute lymphoblastic leukemia (ALL) is one of the greatest successes of clinical oncology. However, cure rates for adults with ALL remain relatively low. Adolescents are the special population between children and adults. These patients group will be received protocol from either pediatric or adult oncologists depending on referral pattern. In recent years, researchers from abroad large cooperative groups show pediatric-based protocol has better outcome than adult-based protocol. In this article, the protocol of ALL in children and adults are compared . It seems the first and probably the most important reason is the dose intensity of the nonmyelosuppressive chemotherapeutic agents, especially asparaginase.

Ten compounds, including five steroidal saponins and five flavonol glycosides, were isolated from the whole plant of Solanum coagulans by means of column chromatographies over silica gel, ODS, Sephadex LH-20, and preparative HPLC. Based on analysis of MS and NMR spectroscopic data, their structures were established as anguiviosides XV (1), smilaxchinoside A (2), methylprotodioscin (3), protodioscin (4), solamargine (5), 3', 4', 5-trihydroxy-7-methoxy-6-C-β-D-glucopyranoside (6), brainoside B (7), camsibriside A (8), kampferol 3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (9), and quercetin-3-O-(2"-β-D-galactopyranosyl)-β-D-glucopyranoside (10). All the compounds were first isolated from this plant. In the in vitro assays, compounds 4 and 5 showed cytotoxic activity against SMCC-7721 and NCI-H460.
Cell Line, Tumor ; Flavonols ; chemistry ; isolation & purification ; pharmacology ; Humans ; Saponins ; chemistry ; isolation & purification ; pharmacology ; Solanum ; chemistry

OBJECTIVE:To discuss the problem in Chinese Traditional Medicine market and provide some countermeasures for its supervision. METHODS: We analyzed the status quo of Chinese traditional medicine market and the reasons accountable for the problem existing in Chinese traditional medicine market. RESULTS & CONCLUSION: The quality of Chinese traditional medicine and Chinese medicinal decoction pieces in Chinese traditional medicine market is far from perfect, it is urgent to tighten supervision on the quality of Chinese traditional medicine.

Objective To evaluate the distribution characteristics of left ventricular systolic dyssynchrony (LV-SD) in dilated cardiomyopathy (DCM) patients with chronic heart failure (CHF) and normal QRS wave width, by pulsed-wave Doppler tissue imaging (PW-DTI), and study its relation with left ventricular systolic function, ventricular remodeling, and functional mitral regurgitation (FMR). Methods The time to peak systolic velocity (Ts) in 12 left ventricular segments was evaluated by PW-DTI, from which the standard deviation (SD) of Ts in the 12 segments (Ts-SD) and maximum Ts difference (Ts-maxD) were calculated. Results Ts-SD and Ts-maxD in the 12 LV segments of the DCM patients with CHF were significantly higher than those of the healthy controls (P＜0.01). In DCM patients with CHF and normal QRS wave width, the incidence of LV-SD was 29.8% (14/47) and the inferior wall was the most frequent distribution site of contraction delay. Linear regression analysis revealed a negative correlation between Ts-SD, Ts-maxD, and left ventricular ejection fraction (LVEF) (P＜0.01), but a positive correlation between Ts-SD, Ts-maxD and left ventricular end-diastolic volume (LVEDV), lefe ventricular end-systolic volume (LVESV), New York Heart Association (NYHA) cardiac function, FMR (P＜0.01) in DCM patients with CHF. Conclusion LV-SD exists in DCM patients with normal QRS width. LV-SD aggravates the LV systolic function damage, which is closely associated with left ventricular remodeling. LV-SD may contribute to the FMR in DCM patients.

Objective To explore the relationship between transitional care model and diabetic patients. Methods 112 patients with type 2 diabetes mellitus were divided into observation group and control group according to random number table. Control group used the doctor as the center patient management mode. The observation group used thepatient-centeredpatient management, application of home nursing service. Results Glycosylated hemoglobin (HbA1c), triglyceride (TG), high-density lipoprotein (HGL), 2 h postprandial blood glucose (2hPBG), fasting blood glucose (FBG), body mass index (BMI) of observation group before treatment respectively were (11.2±2.4)%, (3.7±1.3) mmol/L, (0.5±0.2) mmol/L, (13.6±2.4) mmol/L, (11.8±2.9) mmol/L, (25.9±4.9) kg/m2. Patients discharged from hospital after care for 3 months respectively were (6.8±0.9)%, (1.0±0.3) mmol/L, (2.5±0.4) mmol/L, (8.9±1.5) mmol/L, (6.8 ± 2.0) mmol/L, (20.8 ± 5.8) kg/m2. There was statistically significant difference (t=3.5-6.6,P<0.05). Control group before treatment respectively were (11.9 ± 3.6) %, (3.8 ± 1.5) mmol/L, (0.6 ± 0.3) mmol/L, (13.9 ± 2.9) mmol/L, (11.6 ± 3.2) mmol/L, (25.9 ± 6.8) kg/m2,while patients discharged from hospital for 3 months respectively were (10.9±3.4)%, (3.3±0.7) mmol/L, (0.7±0.2) mmol/L, (12.8±4.2) mmol/L, (10.6± 2.6) mmol/L, (25.1 ± 6.6) kg/m2, there was no statistically significant difference (t=0.05-1.36,P>0.05). Patients discharged from hospital after 3 months, perception, diet, medication, exercise adherence score of observation group was (34.98 ± 5.67), (41.98 ± 5.00), (40.29 ± 5.60), (40.45 ± 7.21) points, which were obviously higher than (21.18±4.75) , (28.46±4.26), (21.88±4.58), (20.98±2.69) points of control group, the difference was statistically significant (t=13.96-19.03,P<0.01). Conclusions Using home nursing service mode in type 2 diabetes care is helping to improve blood sugar levels and improve patient compliance after discharge, fully improve the patients′prognosis.

Ginsenosides (GS)as the main ingredient of ginseng,include dozens of monomer,such as Rg3,Rh2,Rd,Rh1 ,Rg2,etc.GS and various monomers have different anti-hepatoma mechanisms.The main mechanism includes the following aspects,inducing the apoptosis of liver cancer cells,inhibiting the prolifera-tion,invasion and metastasis,reversing the multidrug resistance of liver cancer cells,increasing therapeutic effects by combining with other treatments,improving the immune function of organism and inhibiting hepatic glu-cose production.Different GS monomers play different roles of anti-hepatoma through their respective channels.

Motivational interviewing was a consultation model which centered on patients, and changed patients′unhealthy behaviors by changing their motivations, and promoting their health. Motivational interviewing had been widely used in improving the capacities of patients′self-management with chronic diseases in abroad. Through reviewing the application in chronic disease self-management of motivational interviewing at home and abroad, the paper pointed out the prospect and problems that needed to be solved in our country and provided references for further research on motivational interviewing in self-management of chronic diseases.

Concerning the dilemma between legal and ethical issues in the evolution and development of agential maternity for infertile women,this paper focuses on related ethical issues,including moral conflicts,rights and obligations,and moral evaluation,etc.The realistic value of agential maternity is also explored.

AIM:To study the effects of different duration of sleep deprivation (SD) on neurogranin (Ng),protein kinase C (PKC) and Ca2+-calmodulin dependent protein kinase II (CaMK II) mRNA expressions in hippocampus and forebrain of rats. METHODS:Male Wistar rats were divided into three groups:24 h,48 h and 72 h experimental groups. Each group was divided into sleep deprivation group (SD group) and control group (C group). SD model was established by sleep deprivation box. One step methods by Trizol was used to extract hippocampus and forebrain neuronal total RNA. The changes of Ng,PKC and CaMK II mRNA expressions were detected by SYBRA green I RT-PCR. RESULTS:(1) The expression of Ng mRNA in hippocampus of SD group was significantly lower than that in C group after 24 h,48 h and 72 h SD (P