Drugs, Chinese Herbal ; chemistry ; Drugs, Investigational ; Humans ; Plasma

The endophytic bacteria of the mangroves were studied in this paper. The results show that there are 1.728 (0. 195 -4.225)?104cfu/g (fw) bacterial endophytes in the variety of mangroves, the most population of the endophytic bacteria was found in Rhizophora stylosa, the figure was4. 225?104cfu/g (fw) , the next was Bruguiera gymnorrhiza, Aegiceras corniculatum, Kandelia candel and Avicennia marina. In parts of the mangroves, the contents of the bacteria in stem was the most, the figure was 1. 649?10 cfu/g (fw) , then the root and the leaf. Of the bacteria, about 43. 53% strains expressed the antagonism against the growth of the plant pathogens, such as Fusarium oxyspontm f. sp. cubense , Colletotrichum sp. and Rahtonia solanaceance etc. and these bacteria were identified as Bacillus sp. . The results also showed that 9 of the 13 strains (69. 23% ) could promote the growth of the tomato, while 4 strains (30. 77% ) restrained the tomato's growth.

Objective To investigate the bond strengths of customized titanium bracket manufactured by selective laser melting.Methods Eighty human premolars which had been extracted for orthodontic purpose were collected and divided randomly (by random table) into two groups (customized bracket group and 3M bracket group,40 molars in each group).The 35％ phosphoric acid was used for etching and the brackets were bonded with 3M Unitek bonding adhesive.All bonded specimens were placed in saline for 24 hours at room temperature and were tested on DWD3050 electronic testing machine to determine the shear bond strength and tensile bond strength.After debonding,the adhesive remnant indexes (ARI) were recorded.Results The shear bond strengths of customized brackets was 6.80 (6.20,8.32) MPa,which was significantly lower than that of the 3M brackets [10.46 (9.72,11.48) MPa] (Z =-3.463,P ＜ 0.05).And the tensile bond strengths of customized brackets was (6.93 ± 1.21) MPa,which was significantly higher than that of the 3M brackets [(5.88 ± 1.23) MPa] (t =2.81,P ＜ 0.05).No significant difference was found in the ARI between two different kinds of the brackets.Conclusions The shear bond strength and tensile bond strength of both kinds of brackets were enough for clinic application.

OBJECTIVETo create an integrated digital maxillodental model and to apply it in computer aided design (CAD) of individualized lingual brackets in order to align both crowns and roots without fenestration and dehiscence.

METHODSCone-beam computerized tomography (CBCT)-based maxillodental model and laser-scanned dental model were integrated by auto registration in 10 patients with malocclusions. The registration error was calculated automatically. Three observers tested the method independently. The inter-observer difference was investigated. An integrated model was selected randomly and the setup was created with roots and jaws in good relationship without fenestration and dehiscence. The individualized lingual brackets were designed by CAD on the setup.

RESULTSNo significant difference was found in inter observers (P > 0.05). The registration errors of maxilla and mandible were (0.144 ± 0.020) mm and (0.141 ± 0.022) mm, respectively. The digital individualized lingual brackets based on the virtual treatment in integrated digital maxillodental model were produced.

CONCLUSIONSAn integrated digital maxillodental model was created in good accuracy. By applying the integrated model, individualized lingual brackets were designed.

Adolescent ; Adult ; Child ; Computer-Aided Design ; Cone-Beam Computed Tomography ; Dental Models ; Dentition ; Female ; Humans ; Imaging, Three-Dimensional ; Male ; Malocclusion ; therapy ; Mandible ; diagnostic imaging ; Maxilla ; diagnostic imaging ; Orthodontic Brackets ; Young Adult

Objective To investigate the effects of different concentrations of sodium fluoride on the morphologic characteristics of primarily cultured thyroid cells of SD rats and in order to obtain important proof for approaehing the mechani8m of thyroid gland damage caused by fluoride.Methods Thyroid cells of SD rat were primarily culture for 96 hours,and cell density was adjusted to 5.0×108/L Cell suspension with 5 ml Wills seeded into 6 weII plates,after 12 hours,0(contr01),10.100,1000 μmol/L of sodium fluoride was added into the well, witll each well representing different level of treatment group.Finally the cultured thyroid cells were collected for morph010gic study.Results Under microscope,the transparency of the control thyroid cells Was good,and cells gathered in cluster and adhered to wall.But a lot of cells treated with fluoride suspended,and lost their transparency-under scaning delectron microscope,the control calls showed integrated membrane and tightness to each other,as well as clear boundary between cells normal proliferation.While the thyroid cells treated with 10,100 μmol/L sodium fluoride 0bviouslv shrinked and deformed,and the cells treated with 1000 μmol/L of sodium fluoride were broken-Conclusions nuoride can affect the growth and development of thyroid cell and damage the structure and morphology.Sodium fluoride affects the morphologie characteristics of thyroid cells in a dose-response manner.

Background Researches showed that the incidence rate of cataract is high in the nickel mining area. Nickel sulphate can apparently inhibit the metabolism and proliferation of human lens epithelium cells. But the study on the injury mechanism of nickel on lens is still seldom. Objective Present study was to investigate the effect of nickel sulphate on the lens of SD rats. Methods Forty-five SPF SD rats aged from 7 to 14 days were grouped randomly into subcutaneous injection group, intraperitoneal injection group and blank group. Nickel sulphate of 2 g/L ( 10 mg/kg) was subcutaneously or intraperitonealy injected for 45 days. The opacity of rat lens was examined under the slit lamp at two-week interval and scored based on the criteria of LOCS II and LOCS III. The rats were sacrificed in 45 days after experiment and the lens were obtained for the pathological examination. Result The mean score of the anterior subcapsule opacity of rat lens was obviously higher in subcutaneous injection group compared with blank control group with a significant difference between them (t= 14. 311, P < 0. 05 ) , but no significant difference in the anterior subcapsule opacity between intraperitoneal injection group and blank control group (t = 4. 355 , P>0. 05 ). The score of posterior subcapsule opacity of lens were evidently higher in both subcutaneous injection group and intraperitoneal injection group than the blank control group (t = 9. 316,P = 0. 004;t = 7. 464, P = 0. 009) ,so was the mean score of the anterior +posterior subcapsule opacities(t = 23. 387,P=0. 000;t= 10. 533,P = 0. 002) and the total score of rat lens opacity ( t = 12. 358 , P = 0. 001; t = 10. 188 , P = 0. 003 ) . No significant differences were found in cortex opacity score and nuclear opacity score among three groups ( P > 0.05 ). Histopathology examination revealed that the degeneration of lens collagen protein was more serious in subcutaneous injection group and intraperitoneal injection group than the blank control group,and the injury degree of lens collagen protein was more dominant in subcutaneous injection group. Conclusion System administration of nickel sulphate induced the injury of anterior and posterior subcapsule of lens in SD rat.

Objective To analyze the diagnosis and treatment for complications of renal caliceal diverticulum with calculi or infection. Methods A retrospective investigation was performed on 29 cases with renal caliceal diverticulum. The 29 cases included 11 males and 18 females aged 18 to 61 years. Among the study group, 3 cases were simple renal caliceal diverticulum, 12 cases were diagnosed as diverticular calculi and 14 cases presented recurrent urinary tract infections including 3 cases with urinary fistula after unroofing and decompression as renal simple cyst from another hospital. Ten cases underwent an open operation that unroofed and decompressed the cyst, and sutured the diverticular neck. Eight cases underwent laparoscopic operation similar to the open operation, including lithotomy in caliceal diverticulum in 2 cases. Eleven cases diagnosed with caliceal diverticular calculi were taken one-stage percutaneous nephrolithotomy including dilating the diverticular neck, remaining the nephrostomy catheter and Double-J ureteral stents, and 1 case was transferred to open operation.Results The open and laparoscopic operations were performed successfully. One case was cured by Double-J ureteral stenting after postoperative urinary leakage. One case was transferred to open operation for the failure of percutaneous puncturation. X-ray examination revealed that there were no remaining stones after the operation. All the patients were followed up for 6 to 24 months without calculi and infection recurrence. Conclusions Stones and infection are common that complications of renal caliceal diverticulum. Percutaneous nephrolithotomy, laparoscopy and other operations were effective and feasible treatment options for cases with complications of renal caliceal diverticulum. Exact diagnosis was very important for treatment of renal caliceal diverticulum before operation.

Tomatoes ( Lycopersicon esculentum Mill.) are the principal dietary source of Lycopene which is one of carotenoid and is highly beneficial in preventing some diseases such as the cancer and the heart disease. Suppressing the expression of Lcy gene, the main gene regulating the transformation of the lycopene, is a convenient and effective way to enhance the content of lycopene. The primers were designed according to the gene sequence(U46919)and (X86452) in GenBank. The fruit-specific promoter--phytoene desaturase gene(Pds) promoter and the DNA segment of the Lcy gene were isolated from the genome DNA of tomatoes. The 3'end of Lcy DNA segment was connected together by an intron to inform the RNA interferential segment then which was inserted in the expression vector with the Pds promoter to inform the fruit-specific expression vector. The vector was transformed into the tomatoes through the Agrobacterium tumefaciens. Five transformants were obtained. And the PCR proved that the extra-gene was integrated into the tomato genome. The lycopene in the transgenic tomatoes fruit was increased significantly through analysing the contents of lycopene. These results show that regutating biosynthetic enzyme in carotenoid pathway by RNAi can improve the lycopene content of plant-derived products.
Agrobacterium tumefaciens ; genetics ; Carotenoids ; metabolism ; DNA, Plant ; genetics ; Fruit ; enzymology ; genetics ; metabolism ; Intramolecular Lyases ; genetics ; metabolism ; Lycopersicon esculentum ; enzymology ; genetics ; metabolism ; Oxidoreductases ; genetics ; Plant Proteins ; genetics ; metabolism ; Plants, Genetically Modified ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; genetics ; RNA Interference ; Transformation, Genetic

Abstract: Objective　To analyze the emergency response and long-term intervention effects after the detection of infectious snails epidemic by loop-mediated isothermal amplification (LAMP) assays in Hannan District, Wuhan City, and to explore the application of LAMP in early surveillance and early-warning of schistosomiasis transmission. Methods Snails picked up by the risk monitoring system in Hannan District were examined by anatomical microscopy and LAMP technology to identify the schistosomiasis infection. Emergency response and intensive intervention were initiated in the environment where positive snails appeared, and the long-term effects were evaluated. Results In May 2018, the infectious snails were detected by LAMP technology in Hannan District, and the positive snails were located in Zhujiacha, Dongzhuang Village, Obstacles and weeds were removed and buried by machine in Zhujiacha. 12 700 m2 of snails were killed by drugs, and the mortality rate of snails was more than 80%; no new seropositive persons were found in the emergency examination within 500 m of the positive snail sites. 506 people were examined in Dong Zhuang Village at the end of the year, and 30 positive IHA cases were detected with a blood positive rate of 5.93%, no positive fecal test was found, and all positive blood test patients took preventive medication. The monitoring results of sentinel rats and wild feces were all negative. Health education was carried out, 7 warning signs were deployed and refreshed, and 500 publicity brochures were distributed. After nearly three years of intensified intervention and monitoring in the villages where the positive environment is located, and the density of snails on the stubborn snail has dropped from 0.094/frame to 0.027/frame, and the positive rate of blood test in Dongzhuang Village has steadily dropped from 5.93% to 3.74%. Conclusions The infected snails missed by microscopy were detected by LAMP in Hannan District, which created conditions for the rapid emergency treatment of environment and elimination of positive snail and improved the sensitivity of the surveillance and early warning system in transmission-interrupted areas.

OBJECTIVETo study the development of self-regulation competence and its relationship with behavioral problems in Chinese children with the method of questionnaire assessment.

METHODSThe subjects of the study consisted of 18 to 36 months old toddlers and 3 to 6 years old children. Self-regulation competence in this study included effortful control and self-regulation development. Effortful control is the concept described in Rothbart theory. Attentional focus and inhibitory control were two components in effortful control, which mainly reflect the positive control of emotion and behavior. The Early Childhood Behavior Questionnaire (ECBQ) and Children's Behavior Questionnaire (CBQ) were adopted to assess the attentional focus and inhibitory control of effortful control and impulsivity in children at different ages. Achenbach's Children Behavioral Checklist was used to measure behavioral problems in three to five years old children.

RESULTSTotally 114 18 to 36 months old toddlers and 443 3 to 6 years old children were enrolled in data analysis. (1) The internal consistency of attentional focus in ECBQ and CBQ, alpha (Cronbach) value was respectively 0.89 and 0.75. The internal consistency of inhibitory control, alpha value was respectively 0.84 and 0.78. The alpha value of self-regulation development was respectively 0.84 and 0.94. (2) In 18 to 36 months old toddlers, there was no significant difference between genders in effortful control and self-regulation development. In 3 to 6 years old children, the score of girls' effortful control was higher than the score of boys' effortful control, the scores of girls' self-regulation development factor except regulation motion were significantly higher than boys (t = 4.27 and 11.14, P < 0.05 or P < 0.01). (3) The self-regulation competence including attentional focus, inhibitory control and all the factors in self-regulation development was significantly higher with age in three to six years old children (F = 2.9-10.16, P < 0.05 or P < 0.01). Children in this period already had some regulation skills. The development of regulation competence and regulation skill was not on the same step. Some were fast and some were slow. (4) The scores of attention focus and inhibitory control had significantly positive correlation with the general score of self-regulation development, the coefficient of partial correlation analysis was 0.52 and 0.64, respectively (P < 0.001). (5) The impulsivity had no significant correlation with self-regulation competence in 18 to 36 months old toddlers. The scores of impulsivity and behavioral problems scores had significantly negative correlation with attentional focus, inhibitory control and self-regulation development in 3-to-6 years old children. The coefficients were at low level, none was beyond -0.30 (P < 0.001).

CONCLUSIONSEffortful control or self-regulation had sex differences, girls had higher competence than boys, except motivation of self-regulation. But such sex differences did not show significant difference in children younger than three years of age in this study. This phenomenon is consistent with the development of self-regulation reported by Kopp. Child self-regulation competence increases with age. Generally speaking, the lower self-regulation competent, the higher impulsivity and behavioral problems, but the correlation was at a low level. For assessment and prediction of occurrence of behavioral problems, self-regulation competence should be combined with the other influential factors.

Age Factors ; Child Behavior ; psychology ; Child Behavior Disorders ; epidemiology ; Child Development ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Infant ; Internal-External Control ; Male ; Sex Factors ; Surveys and Questionnaires