Objective To systematically review the efficacy and tolerance of levofloxacin-based rescue regimens for H.pylori eradication failures.Methods A search of Medline,Embase,Cinahl, CBM-disc was performed.Randomized controlled trials comparing levofloxacin-based triple therapy with bismuth-based quadruple therapy were selected for meta-analysis.Assessment of study quality and ex- tracting data to calculate eradication rate and side effect rate.Results Seven randomized controlled stud- ies met the inclusion and exclusion criteria were recruited.Ten-day levofloxacin-based triple therapy was more effective(86.9% vs 61.8% P

The endophytic microorganisms found widely in many kinds of plants mediate various effects to theirs hosts. In this study, seven different dominant endophytes (SDE01 to 07) isolated from a Hy-peraccumulator-Solanum nigrum L. were resistant to Cd2+, and the strain SDE06 survived even in the medium containing 80 mg/L of Cd2+. Bacteria strain SDE06 was identified as Bacillus sp.. The removal of Cd2+ of SDE06 in different conditions were studied. Under the optimal conditions, the incubating time was 36 h, the solution pH 6.0, the temperature was 37?C and the Cd2+ concentration of medium was 20 mg/L, the highest removal rate was up to 80.2% at this condition.

OBJECTIVE: To establish an HPLC method for the determination of Paraquat in biological samples. METHODS: Paraquat in biological samples was extracted by C18 columns which were pre-treated with cetyl-trimethyl ammonium bromide (CTAB) and soudium dodecyl sulphate (SDS), and analysed by HPLC/DAD. RESULTS: The detection limit of the method was 1 ng x mL(-1), and the average recoveries were 81%-94%. CONCLUSION The method can be used to analysis of paraquat in biological samples.
Animals ; Chromatography, High Pressure Liquid/methods* ; Herbicides/chemistry* ; Liver/chemistry* ; Paraquat/analysis* ; Rabbits ; Sensitivity and Specificity ; Sodium Dodecyl Sulfate ; Solvents ; Swine

Objective To study the immunogenicity of human embryonic stem cells(hESCs)and the derived neural stem cells(NSCs)in vitro.Methods The constitutive expression of human leucocyte antigen(HLA)Ⅰ and Ⅱ in hESCs and the NSCs derived from these hESCs were detected by flow cytometry (FCM), as well as the expression of HLA-Ⅰ,Ⅱin NSCs induced by 30 ng/ml recombination human interferon-?(IFN-?).Meanwhile, the NSCs before and after induction of IFN-? were co-cultured with peripheral blood lymphocyte obtained from healthy person.Lymphocyte proliferation standing for the immunoreactivity of NSCs was then investigated.Results The hESCs slightly expressed HLA-Ⅰ(6.18%) and hardly any HLA-Ⅱ before differentiation.However, the NSCs expressed more HLA-Ⅰ(23.56%)as well as HLA-Ⅱ(1.28%, 1.73%)than the hESCs did.Both HLA-Ⅰ(46.43%)and HLA-Ⅱ(8.73%, 10.57%)expressed by the NSCs after they were induced by IFN-? were up-regulated.Conclusions hESCs express certain level of HLA-Ⅰ molecules but do not constitutively express HLA-Ⅱ molecules.The derived NSCs express heavy HLA-Ⅰ and a little HLA-Ⅱ, when treated by IFN-? they can inducibly up- regulated both molecules.The NSCs derived from HESCs are of immunogenicity, which induce rejection aiming at HLA-Ⅰ molecules or even at HLA-Ⅱ molecules when the host is inflammative or under stress, which can result in a failure of cellular transplantation.

In order to develop a rapid method which can check Campylobacter jejuni in animal and poultry foods nicely, an immunomagnetic capture-fluorescent PCR (IMC-FPCR) method was established in this paper. The reported method involves isolation of the target pathogen by immunocapture prior to the fluorescent PCR step, therefore the immunomagnetic-beads for Campylobacter were developed, and two groups of primer/probe, which targeted for the species special sequence of flaA gene and hipO gene for Campylobacter jejuni were designed. The immunomagnetic capture-fluorescent PCR assay amplification of the hipO gene and flaA gene for detection of Campylobacter jejuni was firstly reported in this paper. Result indicated that IMC-FPCR method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 24 h. The assay results are positive for all of the isolates of Campylobacter jejuni (3 isolates, including type strain ATCC 33560 and ATCC8341) with a detection limit of approximately 10 cfu/mL, are negative for Campylobacter coli and several other bacteria. IMC-FPCR assay provide not only a rapid, sensitive method for quantitative detection of Campylobacter jejuni, but also an important method for detecting of Campylobacter jejuni of viable but non-culturerable (VNC) state.
Campylobacter jejuni ; genetics ; isolation & purification ; Fluorescence ; Immunomagnetic Separation ; methods ; Polymerase Chain Reaction ; methods ; Sensitivity and Specificity

Objective To improve ultrasonic unit configuration of the field medical team.Methods The problems of the ultrasonic unit were analyzed in operating platform setup,personnel and facility allocation,application of interventional ultrasound,cross infection prevention and etc,and some countermeasures were put forward accordingly.Results There were interdependence and interaction among the elements of the ultrasonic unit,and the rational configuration made the requirements satisfied for interventional therapy,massive casualty diagnosis,bed side application and etc.Conclusion Improved configuration enhances the efficacy of the ultrasonic unit of the field medical team.

Totipotent and regionally non-specified embryonic stem (ES) cells provide a powerful tool to understand mechanisms controlling stem cell differentiation in different regions of the adult brain. As the development capacity of ES cells in the adult brain is still largely unknown, we grafted small amounts of mouse ES (mES) cells into adult rat brains to explore the survival and differentiation of implanted mES cells in different rat brain regions. We transplanted the green fluorescent protein (GFP)-positive mES cells into the hippocampus, septal area, cortex and caudate nucleus in rat brains. Then the rats were sacrificed 5, 14 and 28 d later. Of all the brain regions, the survival rate of the transplanted cells and their progeny were the highest in the hippocampus and the lowest in the septal area (P<0.01). The grafted ES cells could differentiate into nestin-positive neural stem cells. Nestin-positive/GFP-positive cells were observed in all brain regions with the highest frequency of nestin-positive cells in the hippocampus and the lowest in the medial septal area (P<0.01). mES cells differentiated into end cells such as neurons and glial cells in all transplantation sites in recipient brains. In the hippocampus, the ES cells differentiated into neurons in large amounts. These results demonstrate that only some brain regions permit survival of mES cells and their progeny, and form instructive environments for neuronal differentiation of mES cells. Thus, because of region specific presence of microenvironmental cues and their environmental fields, the characteristics of the recipient tissue were considerably important in formulating cell replacement strategies for neural disorders.
Animals ; Brain ; cytology ; Cell Differentiation ; physiology ; Cell Survival ; Embryonic Stem Cells ; cytology ; transplantation ; Female ; Graft Survival ; Mice ; Rats ; Rats, Sprague-Dawley ; Transplantation, Heterologous ; physiology

Aged ; Antineoplastic Agents ; adverse effects ; therapeutic use ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; Disseminated Intravascular Coagulation ; chemically induced ; Female ; Humans ; Quinazolines ; adverse effects ; therapeutic use

OBJECTIVETo discuss the computer tomography(CT) appearances of the enterocutaneous fistula classification.

METHODSCT scan was performed on 754 patients with enterocutaneous fistula, which were divided into tube fistula and labiate fistula according to clinic classification, and the appearances of CT scan were analyzed respectively.

RESULTSFive hundreds and eighteen patients (68.6%) were diagnosed as tube fistula, and CT appearance of which was a duct formed between internal hole and external hole. Two hundreds and thirty-six patients (31.4%) were diagnosed as labiate fistula,and CT appearance of which was a large external hole like labium.The basic appearance of intestine and celiac cavity in enterocutaneous fistula was inflammatory focus. The incidence of abscesses in tube fistula was higher than that in labiate fistula (P<0.01). The intestinal inflammation was more common in labiate fistula than that in tube fistula (P<0.01).

CONCLUSIONThere are characteristic CT appearances in enterocutaneous fistula and CT scan is useful for classification of enterocutaneous fistula.

Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Intestinal Fistula ; classification ; diagnostic imaging ; Male ; Middle Aged ; Tomography, X-Ray Computed ; Young Adult

OBJECTIVETo prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections.

METHODSThe pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polyclonal antibody (pAb) for DAS-ELISAs. Clinical samples from 186 urogenital infection patients (groups I) and 62 healthy donors (groups II) were detected in parallel by the DAS-ELISAs developed in this study and by IDEIA PCE commercial ELISA.

RESULTSTwo hybridoma cell lines, named 2H4 and 4E6, stably secreting specific mAbs against pORF5 were obtained. The mAb 2H4 was recognized by 32 (17.20%, positive recognition rate) and 25 (13.44%), mAb 2H4 by 0 (0%) and 2 (3.22%) samples from groups I and II, respectively. The sensitivities of mAbs 2H4 and 4E6 were 92.11% and 77.78% and the specificities were 100% and 96.88%, respectively in relation to the IDEIA PCE commercial ELISA. The sensitivities of detection for the DAS-ELISAs were 10 ng/mL (based on 2H4) and 18 ng/mL (based on 4E6).

CONCLUSIONTwo DAS-ELISAs were developed in this study that provided a feasible and effective assay that could be considered alternative tools for the serodiagnosis of C. trachomatis infection.

Adolescent ; Adult ; Chlamydia Infections ; diagnosis ; Chlamydia trachomatis ; pathogenicity ; Enzyme-Linked Immunosorbent Assay ; methods ; Female ; Humans ; Male ; Middle Aged ; Urogenital System ; microbiology ; Young Adult