Objective To investigate the expression of human defensin-alpha1-3 (HNP1-3) in breast cancer and in normal tissues and determine whether there exists a relationship between HNP1-3 expression and clinicopathologic features.Methods Immunohistochemical staining was used to detect the expression levels of HNP1-3 in 59 breast cancer specimens,and 20 normal tissues (fibroadenoma of breast or tumor-adjacent tissues).The relationship of the expression levels of HNP1-3 with patient’s age,clinical stage,histological grade,lymph node metastases and expression of PR/ER/C-erB-2 was analyzed.Results High level expression of HNP1-3 (95%) was expressed in those normal breast tissues.Different expression of HNP1-3 was found in breast cancer specimens and closely correlated with tumor histological stage and lymph node metastases (P

Objective To explore the effect of a training based on instructional video modeling designed from the mirror neuron theory on executive dysfunction after stroke. Methods 40 stroke inpatients with executive dysfunction from July, 2014 to July, 2015 were divided into control group (n=20) and observation group (n=20). The control group accepted executive rehabilitation training, while the observation group were asked to see the video of executive rehabilitation task before training. They were assessed with Behavioral Assessment of the Dysexecutive Syndrome (BADS) before and after intervention. Results The total score and scores of subtests except Rule Shift Cards and Key Search increased more in the observation group than in the control group (t>2.9, P<0.05). Conclusion Rehabilitation video as a visual stimulus for exercise training based on the mirror neuron theory could improve executive function recovery of stroke patients with executive dysfunction.

Objective To develop a more ideal procedure of living donor nephrectomy. Methods Ten cases of living relate donor who received laparoscopic nephrectomy were regularly followed up from 2 to 12 months. Results These donors were discharged on the day 5 after operation. Renal function of the recipients was normal in 4 days after operation. The advantages of laparoscopic living related donor nephrectomy included smaller incisions, less pain, faster recovery and short hospitalization. Conclusion Laparo- scopic living related donor nephrectomy is effective and safe.

Objective To explore the effects of nutritional intervention on body composition,blood biochemical parameters and blood pressure of overweight adults with metabolic syndrome.Methods Forty overweight adults with metabolic syndrome (body mass index (BMI) ＞ 28.0 kg/m2,waist circunference ≥85 cm(for male)or ≥80 cm(for female))participated in this investigation.Changes of serum lipid profiles,blood glucose,blood pressure and other indicators were compaired before and after 2-months'intervention.SPSS 18.0 Software was used for data analysis.Results After two rnonths' nutritional intervention,body weight,body fat,BMI,visceral fat area and waist hip ratio were significantly improved(t =17.02,11.07,5.11,10.35,9.58 ; P ＜ 0.05).The glutamic-pyruvic transaminase,glutamic-oxalacetic transaminease,cholesterol,triacylglycerol,fasting blood-glucose,systolic blood pressure and diastolic blood pressure were also significantly improved after nutrition intervention(t =2.87,2.91,3.33,2.24,2.56,5.74,6.99 ;P ＜ 0.05).Conclusion Nutrition intervention may contribute to improved levels of blood pressure,blood glucose and serum lipid profiles of obesity individuals through body weight and body fat control and lead to reduced insulin resistance caused by obesity.

Objective To investigate the relationship between diastolic function of the left ventricle and common carotid artery (CCA) elasticity in patients with hypertension. Methods Forty-one primary hypertension patients and 31 healthy volunteers underwent echocardiography to obtain the indices of LV diastolic function and also echo tracking techniqne (ET) to obtain the CCA elasticity indices. The LV diastolic function indices included Ve (early diastolic peak velocity), Va (late diastolic peak velocity ), Ve/Va, Em (early diastolic mitral annular motive velicity), Am (late diastolic mitral annular motive velicity) and Em/Am. The indices of CCA elasticity included Ep (pressure-strain elasticity modulus), β(stiffness parameter), AC (arterial compliance), AI (augmentation index) and PWVβ (pulse wave velocity). Results Va was higher and Em, Ve/Va and Em/Am were lower in patients than that in the healthy , P＜0.05. Ep and PWVβ were higher in patients,P＜0.05. Am and Va were correlated positively with PWV β and Ep , respectively in patients, P＜0.05. Ve/Va was correlated positively with AC, and negatively with PWV β, P＜0.05 in patients. Em/Am was correlated negatively with β and PWV β in patients. Conclusions The LV diastolic function and common carotid artery elasticity were decreased in patients with hypertension. The decreased common carotid artery elasticity was along with the LV diastolic function decreased.

Objective To find out the changes of the soft tissue profile in Guangdong women with Angle's I teeth uncovered by lips after extraction of 4 first bicuspid teeth and to evaluate its significance. Methods Fifteen cases of adult Guangdong women with Angle's I teeth uncovered by lips were enrolled in this retrospective study. The X-ray film of lateral cephalometry was used to analyze the soft tissue profile in pre- and post- corrective tooth extraction. The paired t-test was statistically applied for comparison between the changes of lip tissue in pre- and post- correction. Results The changes occurred obviously in the angles of nose vs. lip and upper vs. lower lips, angle Z, and the length, thickness, esthetical plane distance and convex distance of upper and lower lips, but not in the angles of face and facial convex, basic angle of upper vs. lower lips and chin thickness. Conclusilons The facial dislocation and figure of these women have been positively improved after extraction of 4 first bicuspid teeth in spite of the larger thickness of lips than before correction, the just partly shortened distance between lips and the upper and front teeth still exposed and uncovered by lips to some extent. Further improvement depends on functional exercise of lip muscles.

Objective To observe the effect of low doses of ultrashortwave therapy (USW) on sciatic nerve injury and to deduce its possible mechanism.Methods Fifty-four Sprague-Dawley rats were randomly divided into a USW group,a control group and a normal group with 18 rats in each.Each group was then sub-divided into 1 week,2 week and 3 week subgroups with 6 rats in each.A model of peripheral nerve injury was established by forceps clipping of the sciatic nerve in the USW and control groups.The USW group was then treated with USW exposure.Rats from the appropriate subgroups were sacrificed after 1,2 and 3 weeks of treatment.Sciatic nerve samples were stained using hematoxylin-eosin and toluidin blue.Expression of basic fibroblast growth factor (bFGF) was detected by immunohistochemical methods.Results Degeneration of axons was observed in both the therapy and control groups after 1 week,and regeneration at the end of the 2nd and 3rd weeks.The number of axons with myelin sheaths was significantly higher in the therapy group than in the control group at the end of the 2nd and 3rd weeks.The expression of bFGF was significantly higher in the USW group compared with the control group at all observation time points.Conclusion USW can obviously accelerate the regeneration of the sciatic nerve,probably through increased expression of bFGF.

This article introduced the main biological mechanisms of acupuncture promoting nerve function recovery after spinal cord injury, which include inhibition of inflammation and oxidative stress, alleviation of neuropathic pain, increase of neurotrophic active sub-stance, regulation of cell survival/apoptosis gene and neural regeneration pathway.

T he finite element method (FE m) is a mathematical technique using modern computer tech-nology for stress analysis, and has been gradually used in simulating human body structures in the biomechanical field, especially more widely used in the research of thoracolumbar spine traumatology. T his paper reviews the establishment of the thoracolumbar spine FE m, the verification of the FE m, and the thoracolumbar spine FE mresearch status in different fields, and discusses its prospects and values in forensic thoracolumbar traumatology.

AIM: To prepare the efficient tumor-DC vaccines, dendritic cells(DC) derived from 6-8 weeks Balb/c mice bone marrow progenitor cells were pulsed by apoptotic SP2/0 tumor cells and induced maturation by SP2/0 tumor lysates supernatants. Then SP2/0 tumor burdening Balb/c mice were immunized by the tumor-DC vaccines to observe the therapeutic effects in vivo .METHODS: Immature DC were derived by recombinant murine GM-CSF and IL-4, then were pulsed by SP2/0 apoptotic cells. Tumor-DC vaccines were stimulated by LPS and SP2/0 tumor lysates supernatants prepared by four cycles repetitive freezing and thawing, respectively. -thymidine incorporation test and standard 4h [ 51 Cr] release assay were used to detect the proliferation and activation of cytotoxic T lymphocytes (CTL) stimulated by DC in vitro . (4-5)?10 5 DC were immunized in the right inguen of SP2/0 tumor burdening Balb/c mice and most mice received three cycles immunization every two weeks. Changes of the tumor and mice life-spans were recorded. RESULTS: In vitro proliferation and activation of CTL induced by the tumor-DC vaccines of tumor lysates supernatants or LPS stimulation group were more powerful than other groups ( P