1.Relationship between HLA-DRB1 alleles and chronic urticaria with positive autologous serum skin test
Yi WU ; Jinqiu MENG ; Yulin YAN ; Cunwei CAO ; Donghua LIU ; Ling LIANG
Chinese Journal of Dermatology 2012;45(2):78-80
ObjectiveTo investigate the relationship between HLA-DRB1 alleles and chronic urticaria with positive autologous serum skin test (ASST) in Guangxi Zhuang Autonomous Region.MethodsASST was conducted in 144 patients with chronic urticaria,who were subsequently divided into two groups according to the test result:positive group (n =62) and negative group (n =82).PCR amplification with sequence-specific primers was used to determine the genotypes of HLA-DRB1 alleles in the patients and 199 normal human controls.Chi-square test was performed to analyse the difference in the frequency of HLA-DRB1 alleles between the 3 groups by using the SPSS 13.0 statistical software package.ResultsThere were significant differences in the frequency of HLA-DRB1*01,*1401 and *16 alleles among the patients with positive and negative ASST and the controls (x2 =10.92,Pc =0.032;x2 =35.34,Pc < 0.01 ;x2 =12.69,Pc =0.032).Paired comparison revealed significant differences in the frequency of HLA-DRB1*1401 allele between the patients with positive ASST and controls(RR =17.09,Pc < 0.01 ) as well as between the patients with positive and negative ASST (RR =7.20,Pc < 0.01).ConclusionHLA-DRB1*1401 allele may be,or be linked to,the predisposing gene of chronic urticaria with positive ASST in Guangxi Zhuang Autonomous Region.
2.Development and application of Saccharomyces cerevisiae cell-surface display for bioethanol production.
Fei YANG ; Meng CAO ; Yi JIN ; Xiushan YANG ; Shen TIAN
Chinese Journal of Biotechnology 2012;28(8):901-911
Saccharomyces cerevisiae is useful as a host for genetic engineering, since it allows the folding and glycosylation of expressed heterologous eukaryotic proteins and can be subjected to many genetic manipulations. Recent advancements in the yeast cell surface engineering developed strategies to genetically immobilize amylolytic, cellulolytic and xylanolytic enzymes on yeast cell surface for the production of fuel ethanol from biomass. We reviewed the basic principle and progress of S. cerevisiae cell-surface engineering and gave an insight into the recent technological developments in the production of bioethanol using surface engineered yeast.
Biofuels
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analysis
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microbiology
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Ethanol
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isolation & purification
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metabolism
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Fermentation
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Metabolic Engineering
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methods
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Recombinant Proteins
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biosynthesis
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genetics
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Saccharomyces cerevisiae
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genetics
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metabolism
3.The effect of physical training on insulin resistance in patients with chronic heart failure
Zhao-Qiang DONG ; Yi-Meng DU ; Wan-Cai CAO ; Wei-Dong JIANG ; Qun WANG ; Qing-Hua LU ; Le JIANG ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(10):-
Objective To investigate insulin resistance and the effect of physical training on it in the pa- tients with chronic heart failure (CHF). Methods One hundred and twenty NYHAⅡ-ⅢCHF patients were ran- domly divided into a training group( n = 65 ) and a routine therapy group (n = 55 ). Another 35 healthy subjects were recruited as control group. All the patients were treated with routine anti-CHF drugs, and the training group patients had received physical training twice a day in addition. The HOMA-IR, insulin sensitivity index (ISI) , left ventricu- lar ejection fraction (LEVF), left ventricular fractional shortening( LVFS), 6-minute walking distance, heart rate and mean blood pressure were compared between the training and routine therapy groups before and after physical ex- ercise in both groups, and a comparison was made between the patients and the controls before the intervention with regard to HOMA-IR and ISI. Results Comparing with control group, ISI was reduced while the HOMA-IR in- creased (P
4.Residual undifferentiated embryonic stem cells in embryoid bodies
Wei, FU ; Qing-meng, PI ; Lun-gang, SHI ; Zheng-ya, TANG ; Yi-lin, CAO ; Wen-jie, ZHANG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(7):808-812
Objective To explore the residual undifferentiated mouse embryonic stem cells (ESCs) in embryoid bodies. Methods Mouse R1 and Oct-4-GFP transgenic ESCs were firstly cultured in suspension to form embryoid bodies (EBs). Twenty days later, EBs were digested into single cells and then re-plated in standard ESC culture condition. The morphology of residual undifferentiated cells in EBs was observed, and surface makers and in vitro redifferentiation potency of residual cells were examined by flow cytometry and immunofluoreseent staining. The residual cells were expanded and subcutaneously injected into nude mice, and the specimens were harvested from the injection site for histological analysis 6 weeks after injection. Results There were residual undifferentiated ESCs in EBs differentiated for 20 days, which displayed clonal morphology and expressed undifferentiated cell markers of ESCs, including SSEA1, CD31, CD9 and Oct-4. The cells could be differentiated to form EBs again, and could be re-expanded from secondary EBs. The residual cells were able to form teratoma at the injection site, and mature endoderm, mesoderm and ectoderm tissues could be found in teratoma tissues. Conclusion There are residual undifferentiated ESCs after differentiation of ESCs into EBs. The residual ESCs can differentiate again in vitro and in vivo, and can residue again in the in vitro differentiation.
5.Lentivirus packaging, concentration and infection of CD34~+ cells from umbilical blood
Zhi-Dong, WANG ; Chang-Hui, GE ; Wang-Xiang, XU ; Yi-Qun, ZHAN ; Chang-Yan, LI ; Meng-Meng, CAO ; Bo, DONG ; Xiao-Ming, YANG
Bulletin of The Academy of Military Medical Sciences 2009;33(6):522-525
Objective:To methodologically establish the lentivirus granule packaging, concentration and infection against CD34~+ cells from umbilical blood. Methods:The lentivirus system of the 3~(rd) generation was used to produce the virus. Ultrafiltration and ultracentrifugation were employed to concentrate virus. Several treatments were used to improve virus infection including in vitro amplification culture, facilitation of rest cells into cell cycle, promotion of cell adhesion and immobilization during infection, and repeat infection methods. Results:CD34~+ cells were not obviously changed by checking the expression level of CD34 marker on the cell surface after 48 h culture. After two-step concentration, virus titer was increased up to 5.06×10~7/ml, and the infection rate against CD34~+ cells from umbilical blood was increased up to 37.7%.Conclusion:Lentivirus supernatant with over 10~7/ml titer can be obtained using the above methods. Efficient infection against CD34~+ cells from umbilical blood can be achieved.
6.Identification of constituents in vitro and blood-absorbed ingredients of protective effect on acute liver injury from Yin Chen Hao decoction based on UPLC-QTOF/MS
Yi-qing YAO ; Qi CAO ; Xuan WANG ; Hui-lin MA ; Yu-miao CHEN ; Si-yi ZHAO ; Min-xuan GUO ; Jia-meng HU ; Dong-yao WANG ; Di-ya LÜ
Acta Pharmaceutica Sinica 2023;58(5):1173-1180
To identify the active constituents
7.Etiology and clinical features of fever of unknown origin.
Ma JIN-LING ; Cao JIAN ; Wang YU-TANG ; Guo YAN-YAN ; Meng QING-YI
Acta Academiae Medicinae Sinicae 2011;33(1):83-87
OBJECTIVETo investigate the etiology and clinical features of fever of unknown origin (FUO).
METHODSThe clinical data including etiology, diagnostic approaches, and clinical features were retrospectively analyzed in 816 patients with FUO who were presented in our department from January 2000 to January 2009.
RESULTSOf these 816 FUO cases, 766 (93.9%) were confirmed to be with infective diseases(40.4%, n=330), connective tissue diseases (34.4%, n=281), malignant tumors (10.9%, n=89), other known diseases (8.1%, n=66), and unknown diseases (6.1%, n=50). The most common infective disease was tuberculosis (49.7%, 164/330), the most common connective tissue disease was adult-onset Stills disease (AOSD)(55.2%, 155/281), the most common malignant tumor was lymphoma(56.2%, 50/89), and the most common "other known disease" was Crohns disease(22.7%, 15/66). All lung cancer cases had obstructive pneumonia. Significantly more elderly patients suffered from infective diseases (49.4% vs.32.0%) and malignant tumor (15.6% vs. 6.4%) compared with the non-elderly (both P=0.0000), while the proportion of connective tissue diseases was significantly less than that of the non-elderly (17.9% vs. 50.1%, P=0.0000).
CONCLUSIONSMost FUO can be confirmed after careful examinations and analysis. The main cause of FUO is infective diseases, especially tuberculosis in the elderly. The connective tissue diseases and malignant tumors are also important causes.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Connective Tissue Diseases ; complications ; Female ; Fever of Unknown Origin ; diagnosis ; etiology ; Humans ; Male ; Middle Aged ; Neoplasms ; complications ; Retrospective Studies ; Tuberculosis ; complications ; Young Adult
8.Development of research hospital's humanistic system at Nanj ing Drum Tower Hospital
Yitao DING ; Changjiang ZHOU ; Siyao WANG ; Yanling FANG ; Ningchun LIU ; Xuebin ZHANG ; Yanru ZHANG ; Huining YI ; Huiyan LIU ; Hongmei CAO ; Fanrong MENG
Chinese Journal of Hospital Administration 2016;(1):48-51
In the practice of building its humanistic environment of a research hospital,Nanjing Drum Tower hospital adheres to such humanistic characteristics of the hospital as humanistic concept,planning, environment,management,service,and quality.Furthermore,the hospital upholds such keys as learning, innovation, cooperation, undertaking, competition and development. High focus, high starting point planning,persistence,and down-to-earth efforts,effectively promoting hospital development.
9.Pulmonary sarcoid-like granulomatosis induced by aluminum dust: report of a case and literature review.
Hou-rong CAI ; Min CAO ; Fan-qing MENG ; Jing-yi WEI
Chinese Medical Journal 2007;120(17):1556-1560
Aluminum
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adverse effects
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Female
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Granuloma
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etiology
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therapy
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Humans
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Lung Diseases
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etiology
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therapy
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Middle Aged
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Occupational Diseases
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etiology
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therapy
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Sarcoidosis
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etiology
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therapy
10.Research progress on preparation of rat models of acute myocardial infarction
Jue CAO ; kui Yi LI ; qian Meng CHEN ; wu Kui YAO
Chinese Journal of Comparative Medicine 2017;27(10):96-100
Acute myocardial infarction is one of the major causes of death in the world. Research on animal models of myocardial infarction has a great significance in improving the pathophysiology, diagnosis and treatment of human myocardial infarction. At present, problems such as low survival rate and complicated preparation still exist. In recent years, some progress has been made in the preparation of animal myocardial infarction models, which can improve the survival rate, qualified rate and speed. This paper analyzes the preparation of rat models of acute myocardial infarction and related research, combined with actual factors to simplify the process of preparation and improve the skills of scientific researchers, so as to provide a source of stable and reliable acute myocardial infarction models for medical research.