Objective: To study the clinicopathologic properties of hippocampus glioma with temporal lobe epilepsy(TLE) as the main manifestation. Methods: The clinical data of 6 patients with TLE and hippocampus masses were retrospectively analyzed. The 6 patients included 2 males and 4 females, with the longest history of epilepsy being 4 years. Results: All the 6 patients were surgically treated and were pathologically confirmed to have gliomas, including one angiocentric glioma( AG), one pilocytic astrocytoma (PA) with mucous degeneration, one astrocytoma, one oligoastrocytomao, one oligodendroglioma and one glioblastoma. Glial fibrillary acidic protein(GFAP) was strongly positive in all cases. Epilepsy disappeared after surgery in 5 cases; one patient had recurrence and received a second operation. Conclusion: Hippocampus gliomas with TLE as the main manifestation are usually low-grade gliomas of various types, and operation can obtain satisfactory prognosis.

Encephalocele ; Humans ; Infant, Newborn ; Meningocele ; Nasopharynx

Bartter Syndrome ; complications ; physiopathology ; Hoarseness ; etiology ; Humans ; Infant ; Male

Female ; Growth Hormone ; therapeutic use ; Humans ; Infant ; Prader-Willi Syndrome ; diagnosis ; drug therapy ; genetics

Anemia, Aplastic ; drug therapy ; Animals ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Medicine, Chinese Traditional ; Phytotherapy ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Purpura, Thrombocytopenic, Idiopathic ; drug therapy

Hematology ; Humans ; Integrative Medicine

AIM: To investigate the role of retinal pigment epithelium (RPE) in the growth of Müller cells using a co-culture system in vitro . METHODS: Müller cells were cocultured with RPE cells under both normoxic and hypoxic conditions in Transwell chamber culture system. Müller cell proliferation was evaluated by MTT assay. The number of cells which migrate through micropores and stay on the outer bottom side of insert systems were observed and counted. RESULTS: The activities of proliferation and migration of Müller cells when cocultured with RPE cells were significantly higher than those of the Müller cells when cultured alone at all time points under both normoxic and hypoxic conditions. However, for both the coculture and control groups, there is no significant difference between the measurements at 3 and 6 hours. CONCLUSION: Evidence suggests that RPE, when co-cultured with Müller cells, can stimulate migration and proliferation of Müller cells under both hypoxic and normoxic conditions in a time-dependent manner; how-ever, there is no evidence to support the synergetic interaction of RPE and Müller cells co-cultured under hypoxic conditions.

Female ; Humans ; Infant, Newborn ; Kidney Neoplasms ; Twins, Monozygotic ; Wilms Tumor

Brain Neoplasms ; pathology ; Glioma ; pathology ; Humans ; Infant, Newborn ; Male

Background More and more evidences suggest that the interaction of retinal progression of retinal diseases.Objective Present study was to investigate the primarily cultured and digested using explant culture method and trypsas acidic protein(GFAP) staining,S-100 staining and cytokine-18 staining co-cultured under the normoxia and hypoxia using transwell chamber,and the proliferation and migration of cultured RPE cells were examined using MTT and compared with only RPE cells cultured group at 3, 6, 24 and 48 hours.Results Over 90% of primarily cultured RPE cells showed the positive response for S-100.The proliferation and migration of RPE cells were significantly increased in hypoxia condition compared with normoxia condition (P＜0.01).In hypoxia group,amount of proliferation and migration of RPE cells in co-culture group were higher than the only RPE cells cultured group(P＜0.01).Conclusion Hypoxia appear to aggravate the proliferation and migration of RPE cells under the hypoxia status.