Objective: To determine the content of ephedrine in Xiaoer Feiyan Zhike Granule. Meth ods: Chlorimetry is applied, the wavelength is 438 um and the linearity range is 4. 0 - 34. 0mg/L. Results: The recovery is 96. 3% and RSD is 1. 68%. Conclusion: This method is sensitive, specific and accurate and can be used to control the quality of Xiaoer Feiyan Zhike Granule.

Objective This study investigated magnetic resonance imaging (MRI)manifestations and pathological changes after aquaporin-4 (AQP4)gene silencing in treatment of early cerebral infarction.Methods Ninety adult Wistar rats were randomly divided into con-trol group,infarction group and treatment group.All the above groups were assigned to five sub-groups according to the time point:0.5,1,2,4 and 6 hours,respectively (n= 6 for each group).For the right middle cerebral artery occlusion (MCAO-infarction group),the rats were separately injected with short hairpin RNA-AQP4 liposome (shRNA-AQP4+MCAO-control group)and in-terference agents (siRNA-AQP4 + MCAO-interference group).The rats in all groups were examined with T2 weighted image (T2 WI)and diffusion-weighted imaging (DWI).The rs-T2 WI,rs-DWI and relative apparent diffusion coefficient (ADC)of the big-gest high-signal-intensity layer on T2 WI and DWI were measured.Corresponding brain tissue was collected for pathological observa-tion.The data were analyzed using SPSS 13.0 software.Results Identical changes were detected between control and infarction groups.The high-signal intensity was found on DWI at 0.5 hour and on T2 WI at 2 hours after MCAO.rs-DWI and rs-T2 WI in-creased with time.The value of relative ADC decreased quickly within 2 hours after MCAO,and cytotoxic edema was significantly aggravated.The value of relative ADC increased slowly at 4 and 6 hours,but angioedema was detected during this period.There was significant change in relative ADC between the treatment and control groups at 0.5 to 4 hours (P < 0.01)and cytotoxic edema was significantly lightened.But there was no significant difference in relative ADC between treatment and control groups at 6 hours (P >0.05).However,angioedema was visible in this group at 6 hours.Above results confirmed that during early cerebral in-farction,the differential regions of DWI and T2 WI high-signal area were considered as cytotoxic edema,which was the pathologic basis of the decreased ADC values.AQP4 gene silencing could effectively reduce cytotoxic edema.Conclusion DWI and ADC value could reveal timely and exactly therapeutic outcomes of cytotoxic edema.The high-signal intensity on T2 WI represents angioedema, and AQP4 gene silencing has no obvious change.

Objective To investigate the protective effect of Addie on cardiac injury induced by radiotherapy and chemotherapy in patients with cervical cancer.Methods 74 cases of cervical cancer were collected from the oncology department of Zhousha Hospital, those were treated with surgery and radiotherapy and chemotherapy according to postoperative staging and classification of cases.The patients were randomly divided into the experimental group and the control group, with 37 cases in each group.Control group received leukogenic and antiemetic treatment,the experimental group were given Addie injection 100 mL intravenous drip on the basis of the control group, one times per day,10 days as one courses, and two groups were all received 6 courses of treatment.Atthe end of treatment, the condition of heart function, left ventricular ejection fraction, left ventricular end diastolic diameter, 6 minutes walk test, myocardial enzymes, troponin T, C reactive protein /Barhel quality of life score index, EORTC-QLQ score and the incidence of adverse reactions were detected and compared with two groups.Results After treatment, the cardiac function of the experimental group was significantly better than that of the control group (P<0.05).After treatment,LVEDD of two groups decreased (P<0.05), LVEF, 6 minutes walk test distance increased (P<0.05),and LVEDD of experimental group was lower than control group (P<0.05), LVEF, 6 minutes walk test distance higher (P<0.05).After treatment, creatine kinase and creatine kinase levels were increased in two groups (P<0.05).Compared with the control group, the level of creatine kinase and creatine kinase in experimental group were higher(P <0.05).After treatment,the levels of troponin T and C reactive protein in two groups were decreased ( P <0.05 ) .Compared with the control group, the levels of troponin T and C reactive protein in experimental group were lower (P<0.05).After treatment,the scores of Barhel index and EORTC-QLQ score of two groups were significantly higher than before treatment, and the quality of life of the experimental group was significantly higher than that of control group ( P <0.05 ) .There was no significant difference in the incidence of adverse reactions between two groups.Conclusion Addie has an obvious protective effect on cardiac injury induced by radiotherapy and chemotherapy in patients with cervical cancer, and can improve the quality of life of patients.

OBJECTIVE:To evaluate clinical utilization and blood concentration monitoring of vancomycin in our hospital. METHODS: By retrospective survey method, the clinical information (primary diseases, bacteriological culture, blood concentration of vancomycin, clinical efficacy, renal function) of 41 inpatients who had been treated with vancomycin and their blood concentrations from Nov. 2005 to Dec. 2008 were analyzed statistically. RESULTS: The total response rate of vancomycin was 65.0%. The peak concentration of 62.7% cases were below 25 mg?L-1 while the valley value of 55.2% cases were below 5 mg?L-1. The incidence of renal dysfunction after treatment was 12.2%. CONCLUSION: The consumption and blood concentrations of vancomycin are close to low level in our hospital. It should be paid attention to the blood concentration monitoring of vancomycin to achieve an ideal individual administration according to clinical practice.

Objective To analyze the clinical and pathological data of the minimal deviation adenocarcinoma (MDA) of the cervix, and to provide evidence based medicine for diagnosis and treatment. Methods Clinical case reports about MDA or malignant adenoma during 1980-2014 published on CNKI, WanFang, CBM, WeiPu and Medline were retrieved and combined with 2 case with the same diagnosis and treatment. Results Age of onset was 20-86 years old, and the average age was 44.8 years, with median age of 44.5 years. Clinical symptoms of MDA showed massive vaginal apocenosis or irregular bleeding. Gynecological examination common showed cervical hypertrophic and harden. The positive rate of cervical cytology examination was 9.73% (11/113). MDA diagnosed by cervical biopsy and conization accounted for 58.38% (108/185). Immunohistochemical showed that the positive rates of carcinoembryonic antigen, P53 and Ki-67 were 93.91%(108/115), 75.44%(43/57)and 100.00% (42/42). Main operation assisted with postoperative radiotherapy and chemotherapy, early diagnosis, clinical stage and operation methods played important roles in the prognosis. Conclusions The clinical symptoms and physical signs are not obvious for MDA which is difficult to diagnose, so it is easy to diagnose mistakenly and misdiagnose also. The positive rate of cervical cytology is usually lower, repeated and deep cervical biopsy or conization combined with immunohistochemistry can improve the accuracy of diagnosis. The individualized treatment should be selected according to clinical stage for the patients who were confirmed preoperatively.

Objective To design and implement the academic impact power information platform for the secondary disciplines of clinical medicine in order to keep abreast of their advantages and disadvantages. Methods An index system for the assessment of secondary disciplines of clinical medicine was constructed with the bibliometric methods according to the factual data. Results Its functions of query analysis, batch input, and editing of the index data of different disciplines were achieved in higher education institutions using the ASP. NET technology based on the data processing, indexing and statistical analysis. Conclusion The platform can provide the data support for the develop-ment and scientific research management of secondary disciplines, and thus playing an important role in promoting the innovative research and management of clinical medicine.

OBJECTIVE: To probe into the causes of drug repercussion of inpatients in psychiatric hospital to promote rational drug use. METHODS: Using HIS (hospital information system), the drug repercussion data in 8 psychiatirc departments (ward pharmacy) between 2004 and 2005 were analyzed statistically. RESULTS: Of the total 203 drug repercussion cases analzyed, most were resulted from alteration of doctor’s orders, man-made entry errors of drug information, and high drug costs etc. CONCLUSION: The effective way to reduce drug repercussion is to improve technical level and working attitude, establish good rules and regulations and standardize service conditions.

OBJECTIVE:To evaluate the sedative and analgesic effects and safty of fentanyl-propofol and midazolam-propofol by intravenous injection in gastroscopy for aged-patients.METHODS:128aged-patients were randomly divided into3groups:fentanyl-propofol group(groupⅠ),midazolam-propofol group(groupⅡ)and ordinary gastroscopy group(groupⅢ).The effects of sedation and analgesia and the variation of SBP,HR and SDNN were observed.RESULTS:Patients in groupⅠandⅡfelt much less discomfortable than those in groupⅢ(P

TNF in sera from BALB/c mice treated with sepia was detected by L929 cell killing assay. Sera of the mice treated with saline were used as control. Results showed a significant TNF -inducing activity of sepia in animals. And the induced sera were also cytotoxic to human cancer cells,such as GM803 and Y99.

Objective To investigate the role of losartan in AT2-R mediated renal protective effects in renovascular hypertension rat. Methods Male SD rats were divided into sham group and renovascular hypertension group in which Goldblatt methods were performed. The two groups were intragastically administered with losartan (respectively 0, 10, 20, 30 mg?kg-1?d-1) for 6 weeks. Then renal function was measured, the AT2-R expression in the non-ischemic kidney and the AngⅡ contents in non-ischemic kidney and plasma were determined. Results In renovascular hypertension rats, especially in the contralateral, non-ischemic kidney, the expression of AT2-R protein were up-regulated. Losartan increased the AngⅡ contents in non-ischemic kidney and plasma, mediated the natriuretic/diuretic effect and renal protective effect in renovascular hypertension rat and increased the expression of renal AT2-R. Conclusion In renovascular hypertension rat, losartan mediates renal natriuretic/diuretic effect and protects renal function through up-regulating AT2-R in kidney.