Triple negative breast cancer(TNBC) is defined by the lack of estrogen receptor (ER) , progesterone receptor (PR) and human epidermal growth factor receptor-2 ( Her-2) expression. It is a subtype of tumor with distinct clinical and pathologic features and lacks effective targeted therapies. It is non - sensitive to endocrine therapy and targeted therapy drug herceptin, leaving chemotherapy as the mainstay of treatment The feature and the treatment of TNBC is the hotspot of resent research.

Glycogen storage diseases (GSD) are a group of inherited disorders characterized by enzyme defects that affect the glycogen metabolism. Hypoglycemia, hepatomegaly, splenomegaly and growth retardation are the main clinical manifestations. The enzymes affecting glycogen synthesis and degradation are varied.Consequently, the clinical manifestations are different because of different enzyme defection. Most patients treated regularly can obtain the normal growth and grow to adults, but still can be accompanied long-term complications by adulthood. Now some scientists find new modified cornstarch is superior to standard therapy in maintaining blood glucose levels. Gene therapy is still in the experimental animals.

C-termini of proteins often play an important role in various biological processes, such as the transcription and translation from DNA to protein and also participating in various biological regulations. The determination of protein C-terminus is so crucial because it provides not only distinct functional annotation, but also a way to monitor the proteolysis-modified proteins. Based on the biological mass spectrometry, a series of novel methods and technologies were developed both for qualitative and quantitative analyses of protein C-terminus. These methods or technologies can be applied to accurate and effective protein C-terminus profiling, including the sequences and quantitative information of C-termini, which reveals the biological function of C-termini in life's activities and provides a better understanding of the degradation of mature proteins. Combined with our research, this review highlights the improvements in C-terminal proteomics study in the past decades, including the methodologies for recognition and identification of C-terminus, as well as the enrichment strategies for protein C-terminus.
Mass Spectrometry ; Protein Processing, Post-Translational ; Proteins ; chemistry ; Proteolysis ; Proteomics

Objective To investigate the incidence of adult epilepsy patients with attention-deficit hyperactivity disorder (ADHD) and the characteristics of ADHD subtypes, and to explore the correlations between several epilepsy-related factors with ADHD.Methods According to corresponding inclusion criteria and exclusion criteria, 156 adult epilepsy patients who visited to Neurology Clinic of Zhejiang Hospital and the First Affiliated Hospital of Anhui Medical University from March 2014 to October 2014 were enrolled as a study group to enter this test.Eighty-six nomal adults were enrolled as the control group who matched for the age, gender, inteligence quotient with the study group.Using the chi-square test and Logistic regression analysis and the other statistical methods to analyze the phenomenon of ADHD in adults with epilepsy and the factors that may contribute to the phenomenon.Results The detection rate of ADHD in 156 adult patients with epilepsy was 26.9％ (42/156), significantly higher than that in the heathly control group which was 4.7％ (4/86, x2 =17.862, P ＜ 0.05).Symptomatic epilepsy and the electroencephalogram showed multifocal discharge had higher detection rate of comorbid ADHD and the difference was statistically significant (The detection rate of idiophathic epilepsy was 22.1％ (21/95), symptomatic epilepsy was 46.7％ (14/30), cryptogenic epilepsy was 22.6％ (7/31), x2 =7.362, P =0.025.The detection rate of frontal epileptiform discharges was 30.6％ (11/36), temporal area was 25.9％ (14/54), central area was 18.2％ (4/22), multifocal epileptiform discharges was 46.2％ (12/26), the normal was 1/18, x2 =10.187, P =0.037).The results of binary Logistic regression analysis showed that epilepsy age of onset (B =-0.889, P =0.049) was possibly an important factor for epilepsy with ADHD.Conclusions The risk of ADHD occurred in adults with epilepsy is higher than that in the healthy adults and attention-deficit is the common subtype.The etiology, age of onset and electroencephalogram features of adults with epilepsy may be associated with the occurrence of ADHD.

BACKGROUND:Precancerous lesions are a long-term development process in which many factors are involved. Bone marrow mesenchymal stem cel s can repair tissue injury. OBJECTIVE:To investigate the effect of bone marrow mesenchymal stem cel transplantation on gastric precancerous lesions in the rats. METHODS:Thirty-six Wistar rats were randomly divided into control group, model group and transplantation group. Animal models of gastric precancerous lesions were established in the model and transplantation groups. Rats in the transplantation group were given 1 mL of CM-dil-labeled bone marrow mesenchymal stem cel s (3×106 cel s) via the tail vein, once a week, total y three times. Rats in the model and control group were subjected to the tail vein injection of the same volume of normal saline. Then, rats were sacrificed 1 week after final injection, and pathohistological changes in rat gastric tissue sections were observed. The expression of vascular endothelial growth factor in the gastric mucosa and levels of serum cytokines were detected. RESULTS AND CONCLUSION:The severity of gastric mucosal injury in the transplantation group was lighter than that of the model group. The expression of vascular endothelial growth factor was significantly higher in the transplantation group compared with the model and control groups (P<0.05). The levels of serum interleukin-17 and interferon-γwere significantly higher in the model group than the transplantation group fol owed by the control group (both P<0.05). Therefore, bone marrow mesenchymal stem cel s can increase vascular permeability, reduce inflammation, block or ease the occurrence of precancerous lesions by up-regulating the expression of vascular endothelial growth factor in the gastric mucosa lesions and reducing the expression of interleukin-17 and interferon-γ.

In this paper, the concept, policy support and existing modes of transitional care in America were introduced in detail. It can provide reference for the development of transitional care mode in our country, provide continuous nursing services for the elderly, and improve the health status of the elderly.

[ABSTRACT]OBJECTIVETo evaluate the efficacy of combined oral and intratympanic glucocorticoids treatment for severe to profound sudden sensorineural hearing loss(SSNHL).METHODSFifty-four patients with severe to profound SSNHL were retrospectively studied. They were treated with oral steroids, vasodilators, anticoagulants. The patients who accepted intratympanic methylprednisolone sodium succinate were included the therapy group, the others were included in the control group. Hearing improvements were analyzed respectively after two weeks.RESULTSHearing in both groups were improved significantly after treatment(P<0.01), but there was no difference in hearing improvements between two groups(P=0.194). The efficacy for severe SSNHL patients in therapy group was equal to those who were in control group (P=0.251), so was the efficacy for profound SSNHL patients in both groups(P=0.380).CONCLUSIONThe combined systemic and intratympanic glucocorticoids treatment was effective for patients with severe to profound SSNHL, but while with no better efficacy than oral glucocorticoid alone.

Obiective To explore the application of psycho-cardiology in patients with chronic heart failture compli-cated with depressive disorder. Methods Hospitalized patients with chronic heart failture were assessed with PHQ-9 and PHQ-2 health questionnaire. A total of 80 patients with depressive disorder were selected and randomly divided into routine treatment group (n=40, received routine medication) and intervention group (n=40, application of psycho-cardiology based on routine medication). After three months, changes of scores before and after therapy were statistically analyzed in these two groups. Results Compared with routine treatment group, total scores of PHQ-9 significantly decreased in intervention group (P＜0.01). LVEF, BNP and CRP improved in both groups after intervention (P＜0.05), which were more prominent in intervention group compared with routine treatment group (P ＜ 0.05). Conclusion Psycho-cardiology can treat chronic heart faiture and solve psychological problems of patients and provide comprehensive rehabilitation to patients.

The irrational drug use is one of the major public health issues. As children are in growth, their drug absorption, distribution, metabolism, excretion and pharmacodynamic characteristics are different from those in adults, thus more challenges on how to make rational use of drugs in children are seen. In this paper, by analyzing current situation of irrational drug use in children, the definition and standards of the rational drug use, and the measures adopted by WHO and China in promoting rational use of drugs in children, we emphasize here and call for a promotion of the awareness of rational use of drugs in children, and activities in forms of propaganda and health education, popularization of medical science and the medication safety knowledge, guidance for the development of good medication beliefs and habits in public, improvement of the social security administration, maximization of the protection of children's medication safety, and maintenance of children's health rights and interests.

Objective To investigate the relationship between body mass index(BMI) and the outcomes of liver function of patients with hepatocarcinoma after surgery. Methods We divided the patients into three groups: normal, overweight and obese groups based on BMI, then we recorded their preoperative and the postoperative liver functions: albumin, total bilirubin, alanine aminotrasferase(ALT), aspertate aminotransferase (AST), alkaline phosphatase(ALP), γ-glutamyltranoferase(γ-GGT), triglycerides(TG), total cholesterol (TC), high density lipoprotein(HDL) and low density lipoprotein(LDL). Results The mean values of ALT change between the preoperative and the postoperative were (14.9 ± 1.98) U/L, (15.4 ± 2.27) U/L,(22.2±1.32) U/L; Mean values of γ-GGT change were(16.2 ± 1.33) U/L, (18.7 ±0. 97) U/L,(22.3 ± 1.27) U/L; mean values of TG change were (0. 18 ± 0. 44) mg/dL, (0. 21 ± 0.08) mg/dL,(0.25 ± 0.03) mg/dL. Compared with the results of control groups, there were no significant differences of recovery of liver functions between normal and overweight groups(P ＞ 0. 05), but the obese group' s recovery of liver functions did lag behind the normal group (P ＜ 0.05). It is showed that the obese group' s shared same recovery of albumin, total bilirubin, AST, ALP, TC, HDL and LDL. The difference was significant between the normal and obese groups in terms of recovery of ALT, γ-GGT, TG(P ＜ 0. 05). Conclusion BMI is associated with the recovery of liver function of patients with hepatocarcinoma, the obese patients have poor prognosis of recovery of liver function.