OBJECTIVETo explore the alterations of after depolarization and triggered activity in myocardiac muscle during progression of mouse aortic stenosis.

METHODSAfter the establishment of mouse aortic stenosis model, the heart was collected and intracellular potential was recorded using standard glass microelectrode. After the recording, the action potential (AP), early after depolarization (EAD), delayed after depolarization (DAD) in papillary muscles were analysed.

RESULTS(1) Compared with that of time-matched control mice, there were no changes of the action potoutial duration at 90% repolarization(APD90) in model mice at 2 and 5 weeks, whereas the APD90 was progressively prolonged at 9 and 13 weeks. (2) During the 30 minites of the experiment, spontaneous EADs and DADs were frequently present in medel mice at 9 and 13 weeks, were not in control and model mice at 2 and 5 weeks. (3) Compared with those of control group, EADs and DADs triggered by hypokalaemia or isoproterenol were increased significantly. The incidence rate of triggering EADs and DADs was progressively increased over time.

CONCLUSIONEAD, DAD and trigger activities increase gradually during the process of aortic stenosis, and it suggests that the myocardium electrical-instability is markedly increased in the insult hearts.

Action Potentials ; physiology ; Animals ; Aortic Valve Stenosis ; physiopathology ; Disease Models, Animal ; Male ; Mice ; Myocytes, Cardiac ; physiology

OBJECTIVETo find the characteristic parameters from electrocardiogram (ECG) which is most related to pathological progress, surface ECG was performed in mice at 2, 5, 9 and 13 week post surgery.

METHODSElectrocardiogram recordings: The mice were anaesthetized with tribromoethanol (250 mg/kg, i.p.). Lead II surface ECG were acquired by using the Biopac System MP150 at a rate of 5 kHz.

RESULTS(1) No arrhythmia was observed in Sham and 2-week Band mice. Spontaneous arrhythmias were observed in ECG recordings, with an incidence of 15%, 28% and 63% in 5-, 9- and 13-week Band mice, respectively. Tachyarrhythmias, such as frequent premature ventricular extrasystole, bigeminy, trigeminy, and paroxysmal tachycardia were found. (2) Analysis of ECG recordings revealed a significant prolongation of QT and QTc intervals. Compared with age-matched Sham mice, the increment in QT and QTc intervals was 20.4%, 32.7%, 49.7%, 61.0% and 27.1%, 32.1%, 43.9%, 59.1% at 2, 5, 9 and 13 week, respectively (P < 0.01). (3) The characteristic change in electrocardiogram was on the J wave. The amplitude of J wave was upward in Sham mice, and it was significantly flattened or inverted in Band mice. (4) Except that RR interval at 2 week showed a slight decrease, there was no significant change in PR interval and RR interval in Band mice.

CONCLUSIONIn the present study, we found that the increase incidence of spontaneous arrhythmias, prologation of QT interval and changes in the amplitude of the J wave in the surface ECG during progression of mouse cardiac hypertrophy and failure, and the progressive pattern indicated that a gradual aggravation of the ventricular repolarization delay in this mouse model.

Animals ; Cardiomegaly ; physiopathology ; Disease Models, Animal ; Electrocardiography ; Male ; Mice

Objective:To investigate the feasibility of 3-D computer aided design(CAD)for the framework of a mandibular Kennedy Class Ⅱ,Modification 2 arch.Methods:In terms of the principle of clinical design,the 3-D framework was built with the technique of mixed model of points cloud,curves and surfaces on the reconstructed model of a partially edentulous cast,including the tissue surfaces and polishing surfaces of every component,such as clasp,rest,lingual bar and mesh construction,etc.Results:Preliminarily,a CAD method for 3-D RPD framework design was established and a individualized RPD framework 3D geometric model was set up.Conclusion:CAD for RPD framework design based on the mixed model of points cloud,curves and surfaces is feasible.

Objective To investigate the relationship between cross‐sectional area (CSA) and average CT value of posterior vertebral extensor group and bone mineral density(BMD) in postmenopausal women .Methods Totally 114 postmenopausal women being eligible for inclusion were enrolled .All the subjects ,ages range from 55 to 87 years old ,underwent quantitative computed tomography(QCT) scans from December 2015 to December 2016 in Beijing Jishuitan Hospital .The original image was transmitted to the image postprocessing workstation ,using Mindways QCT Pro software ,vertebral bone mineral density of L2-L4 were meas‐ured .CSA and average CT value of bilateral posterior vertebral muscles were measured by 2 diagnostic physicians in the L4 verte‐bral body horizontal axis CT images ,and analyzed respectively .Results Average CT values of posterior vertebral muscles between the left and the right side had a significant difference (P<0 .01) .The average CT value of bilateral posterior vertebral muscles was positively correlated with lumbar bone mineral density measured by QCT(P<0 .01) .There was no significant correlation between the CSA of posterior vertebral extensor group and BMD .Conclusion The loss of posterior vertebral muscle mass may be an impor‐tant risk factor for osteoporosis .The average CT value of posterior vertebral extensor group is more promising than CSA in the e‐valuation of the degenerative muscles .

Double template molecularly imprinted polymer ( D_MIP) was prepared by emulsion polymerization method using the nonylphenol (NP), bisphenol A (BPA) as templates, α_methyl methacrylate(MAA) as functional monomer. Then using the D_MIP as solid phase extraction materials, a method based on double_molecularly imprinted solid phase extraction coupled with high performance liquid chromatographic fluorescence detection was developed for the determination of trace NP and BPA from environmental and food samples. The D_MIP was characterized by Fourier transform infrared spectroscopy ( FT_IR ) and adsorption experiments. The results showed that the saturated adsorption capacity of the D_MIP for NP and BPA was 73. 3 and 97. 5 mg/g, respectively, and the relative selectivity coefficient was 2. 2 and 1. 7, respectively. Moreover, the conditions affecting extraction efficiency, such as washing conditions and desorption conditions were optimized. Under the optimal conditions, there was a good linear correlation in the concentration range of 0. 01-2. 3 mg/L with correlation coefficients (R2) greater than 0. 998 for NP and BPA. The detection limits ( S/N=3) were 0. 001 and 0. 002 mg/L. The proposed method was applied to determine NP and BPA in river water, beer and crucian carp samples. The recoveries of NP and BPA were in the range of 86. 4%-99. 1%with the relative standard deviation less than 6 . 2%. This method has good selectivity and high sensitivity and shows good prospects for the application in the enrichment and separation of NP and BPA in real samples.

TheStandardized Manipulations of Acupuncture and Moxibustion has been published and gradually standardized the clinical practice. It provided a solid foundation for the standardization of acupuncture and moxibustion therapeutic effects. It also provided powerful convictable effects on the accuracy and scientificity of acupuncture and moxibustion clinical practice, which gained well-acceptance from patients. In the treatment of certain diseases, acupuncture and moxibustion have become irreplaceable so that acupuncture and moxibustion can be strongly promoted both at home and abroad. There were some common problems occurred during the establishment of the national standardization onStandardized Manipulations of Oral and Lip Acupuncture. This article reviewed, analyzed and discussed questions occurred during the standardization establishment. The results showed that according to the principle of standardized production, a rigorous, scientific and reasonable text standard can be established. Advices or opinions gained during the process can be used as references for future standardization establishment. It was concluded that the standardization of acupuncture had great guidance in the acupuncture clinical application. It is an arduous and long-term mission to achieve and strengthen the position of international standardization of acupuncture and moxibustion. The government should increase the effort on investment and promotion of acupuncture and moxibustion standardization. Conversation and cooperation should also be strengthened with international communities to promote the standardization implementation.

Aged ; Anthracosis ; physiopathology ; Autonomic Nervous System ; physiopathology ; Death, Sudden ; Humans ; Male ; Middle Aged

Objective To study the biological effects of grape seed proanthocyanidin extract (GSPE) on human ovary cancer cells and to explore the molecular mechanism. Methods Human ovary cancer cell line SKOV3 were co-cultured with GSPE solution of the terminal concentrations of 25,50,100,200,400 ?g/ml respectively in 96-well plate. At 24,48,72 h after coculture,the following parameters were detected: cell growth curve,the inhibition rate of SKOV3 cells by MTT assay,DNA cycle by FCM,the apoptosis of SKOV3 by TUNEL and Annexin-V labeling method,the mRNA and protein expressions of survivin by semi-quantitative RT-PCR and Western blotting,respectively. Results GSPE dose-dependently inhibited the proliferation of the SKOV3 cells. Treated by GSPE,the progress of SKOV3 cells at S stage into G/M stage was inhibited. TUNEL showed that treated by 25 ?g/ml GSPE for 24,48 h,the apoptotic rates of SKOV3 cells were 31.98%,45.78% respectively. Annexin-V showed that after incubation with 25 ?g/ml GSPE for 24 h,the apoptotic rate was 14.68%. The survivin mRNA and protein expressions were both down-regulated. Conclusion GSPE inhibits the proliferation of malignant human ovary cancer cells and induces their apoptosis. Expression of survivin mRNA and protein may be related to cell growth inhibition and to the apoptosis mediated by GSPE in vitro.

Objective To investigate the effects of glycyrrhizin on gene expression of transforming growth factor(TGF)-?signaling pathway of hepatic stellate cell(HSC)in mice by gene microarray tech- nique.Methods The HSCs were isolated from mice and cultured in vitro.Then the mice were divided into control group,TGF-?_1 group(5 ng/ml) or TGF-?_1(5 ng/ml)combined with glycyrrhizin(100?mol/L) group.The cells were collected after 10 hours to extract RNA.A cDNA microarray(GEArray~(TM) Q) targeting TGF-?/BMP signal transduction was used to screen the genes which showed significant changes in expression of TGF-?pathway of HSC by glycyrrhizin.Results The microarray analysis showed that 16 genes(16.7%),such as Smad2,Smad3,Smad7,CoL3A1,CoL1A2,and PAI-1,were upregulated by TGF-?_1 and then down-regulated by glycyrrhizin.Five genes(5.2%)(including BMP7,IGFbp3 and etc.)downregulated by TGF-?_1,were then up-regulated by glycyrrhizin.Finally,2 genes upregulated by TGF-?_1 were then up-regulated predominantly by glycyrrhizin in HSC(T?R2,betaglycan).Changes in some genes,such as Smad2 Smad3,Smad7,were further confirmed to be coincided with cDNA microarray by semi-quantitative RT-PCR.Conclusions The anti-fibrosis mechanisms of glycyrrhizin may be through to interference of TGF-?signaling pathway,decrease the synthesis and increase the degradation of collagen.

Objective To investigate the new epidemiology and clinical manifestation of measles in children in recent years. Methods One hundred and twenty-eight cases of children′s measles were collected,and their epidemiological and clinical characteristics of age distribution,endemic distribution,vaccination,clinical manifestation,complication and misdiagnosis were discussed. Results Among the patients living in urban,90% cases were single dose vaccination,35% cases were between 3-8 years old,27% cases were less than 8 months old,59% cases were mild measles,27% cases were atypical measles,40% cases were misdiagnosed at first visit,and 67% cases of measles less than 8 months old were babies of vaccine immunized mother.Among the patients living in rural,68% cases had no vaccination,86% cases were at age of typical onset,61% cases were typical measles,7% cases were severe measles,and 28% cases were mild measles.Among all cases of measles,mild measles and atypical measles were as high as 41% and 16%,but typical measles were only 37%.In all patients,56% cases had typical measles rash,82% cases had Koplik′s spots,77% cases had typical onset of eruption,100% cases had higher fever in eruption stage,89% cases had typical downward sequence of eruption,93% cases had skin pigmentation,and 81% cases had desquamation. Conclusions Less than 8 months infants and single dose vaccinated pre-school children are newly susceptible group of measles.Mild measles and atypical measles markedly increased,with atypical clinical symptoms,variable rash and variant onset,but the character of aggravated fever in eruption stage scarcely changes.Infants of vaccine immunized mother and pre-school children are advised to vaccinate ahead of schedule.