Based on TCM relative diagnostic theory and clinical experience,the author put forward that atrophic glossitis is caused by yin deficiency and should be treated from yin deficiency.During the past long-term clinical practice,the treatment from Yin deficiency has been proved a sound effect in the therapy of atrophic glossitis.

Objective To investigate the effect of adenosine on rats with acute renal failure(ARF) resulting from ischemia-reperfusion(IR).Methods The model of renal IR injury rats was induced by nipping bilateral renal pedicels of rats with non-traumatic forceps for 40 minutes.The first adenosine group was given adenosine 10 mg/kg intraabdominally at 2 hours before IR.The second adenosine group was given the same adenosine at 40 minutes after IR.Serum creatinine(Scr) levels,the proportion of apoptosis of renal tubular cells(RTCs) and the pathological changes of renal tissue were observed separately in every group at 2,6,12 hours after IR.Results 1.At 12 hours after IR,in IR group Scr levels and apoptosis of RTCs count were(198.56?13.35) ?mol/L and 26.50?2.07,which were increased significantly compared with sham group.In the first and second adenosine groups,they were(113.85?(12.90) ?mol/L),14.67?3.44 and(218.79?37.65) ?mol/L,20.83?5.27,respectively,and all of them were higher than those in sham group(all P0.05).Conclusions Adenosine which is given to rats with IR before IR may be an effective pharmacological agent for prevention and treatment of renal IR injury.The mechanism may be relevant to saving energy and reducing cellular apoptosis.

Objective To observe the effects of baicalin on forms of hepatic tissue, liver apoptosis, mRNA expressions of iNOS, NF-κB and protein expression of Caspase-3 in rats with ischemia reperfusion injury; To discuss its mechanism of action.Methods The rat models of liver ischemia reperfusion were performed according to the Pringle's method. Rats were randomly divided into sham-operation group, model group and baicalin group. Sham-operation group and model group were given normal saline for gavage, while baicalin group was given baicalin for gavage. Morphological characteristic was observed by HE staining. Hepatocyte apoptosis was determined by TUNEL. The mRNA expressions of iNOS and NF-κB were determined by RT-PCR. The protein expression of Caspase-3 was determined by Western blot.Results Compared with the sham-operation group, mRNA expressions of iNOS and NF-κB and the protein expression of Caspase-3 in the model group increased, as well as liver apoptosis rate (P<0.05,P<0.01); compared with the model group, mRNA expressions of iNOS and NF-κB and the protein expression of Caspase-3 in the baicalin group decreased, as well as liver apoptosis rate (P<0.05), and the hepatic lesion significantly improved in the baicalin group.Conclusion Baicalin can restrain Caspase-3 induced apoptosis by reducing the expressions of iNOS and NF-κB, with a purpose to realize the hepatoprotective effect for liver of rats with ischemia reperfusion injury.

Objective To cvaluate the efficacy and safety of HabibTM VesOpen intravascular radiofrequency catheter percutaneous radiofrequency ablation combined with TACE in the treatment of portal vein tumor thrombus of primary hepatocellular carcinoma.Methods Thirty-nine patients with primary hepatocellular carcinoma complicated with portal vein thrombosis were treated with HabibTM VesOpen intracavitary catheter ablation combined with TACE.The postoperative success rate,complication,blood index,α-fetoprotein (AFP),portal vein patency and tumor thrombus imaging changes were assessed.Results All of 39 patients underwent surgery successfully without occurrence of perforation,infection,hepatapostema,intra-abdominal hemorrhage nor other complications associated with surgery.Blood routine examinations (WBC,RBC,PLT) after 2 weeks and 4 weeks of surgery had no statistical difference compared with those of preoperation.Liver function indexes including ALT,AST,ALB had statistical difference before and after the surgery except for TBIL.AFP had statistical difference before and after surgery.Doppler ultrasonography after 4 weeks showed blood flow in original blocked portal vein.Enhanced CT or MR examinations after 8 weeks on the abdomen,suggested that tumors were shrinked or disappeared.Conclusion Radiofrequency catheter ablation with intracavitary radiofrequency catheter of HabibTM VesOpen is an effective method in the treatment of portal vein tumor thrombus in primary hepatocellular carcinoma.

ObjectiveTo study the toxic effect of bupivacaine encapsulated in liposomes on spinal cord in rats.MethodsOne hundred and eight SD rats (200-225 g) in which intrathecal (IT) catheter was successfully implanted without complications were randomly divided into 6 groups ( n =18 each):control group (group C) ; liposome group (group L) ; 0.5％ and 1.0％ bupivacaine groups (groups B1 and B2 ) and 0.5％ and 1.0％ bupivacaine encapsulated in liposomes groups (groups LB1 and LB2 ).In groups L,B1,B2,LB1 and LB2,liposome,0.5 ％ bupivacaine,1.0 ％ bupivacaine,0.5 ％ liposomal bypivacaine and 1.0 ％ liposomal bupivacaine 20 μl were injected IT respectively once a day for 7 consecutive days,while in group C nothing was injected IT.Pain threshold was measured by mechanical stimulation of the plantar surface of hindpaw.Motor function of the hindlimbs was also assessed.The animals were sacrificed at 8 day after IT injection.The lumbar segment of the spinal cord was removed for microscopic examination,detection of neuronal apoptosis (by flow cytometry) and Fos protein expression (by immuno-histochemistry).Results1.0％ bupivacaine IT significantly increased the percentage of apoptotic neurons in group B2 as compared with control group.0.5％ and 1.0％ bupivacaine IT significantly increased the number Fos protein positive cells in group B1 and B2 as compared with group C.1.0％ bupivacaine IT induced severe histologic damage including shrinkage of nucleus and vacuole formation in mitochondria.Encapsulation of bupivacaine in liposomes significantly attenuated bupivacaine-induced increase in apoptosis and Fos protein expression and histologic damage in group LB2 as compared with group B2.ConclusionThe encapsulation in liposomes can decrease the neurotoxicity of 1.0 ％ bupivacaine administered IT in rats.

Objective To know the drinking water quality in the rural areas in Fujian Province in order to provide the scientific basis for water improvement in the rural areas.Methods Fourteen counties were selected in Fujian,2004,10 monitoring sites in each county based on the population proportion of different drinking water types.Sixteen indicators were determined for every water sample.Results In the 14 investigated counties,38.71% of total population drinking water supplied with surface water,and 61.29% with ground water source.Drinking water from centralized water supply system was supplied to 58.24% of total population.The qualified rate of water samples was 26.39% for total 288 water samples,35.38% for surface water samples and 18.99% for ground water samples respectively.The hygienic state of surface water was much better(P

Objective To induce a line of Plasmodium berghei with resistance to artemisinin. Methods The major methods included blood transmission from passage to passage and progressive increase of drug pressure. Results The resistant lines were developed by different protocols: (A) The initial dosage of artemisinin was 126\^2 mg/kg which was increased by 60 mg/kg for the next passage and boosted by 126\^2 mg/kg for every other passage. As developed to passage 60 and 76, the resistant index was 18\^39∶1 and 14\^89∶1 respectively, then decreased gradually. For passage 108, the dosage was 8 862\^5 mg/kg, but the resistant index was only 10\^49∶1. (B) Using passage 66 from (A) as the source, a dosage of 4 000 mg/kg was given each week, the resistance of the passage 40 increased significantly with an index of 27\^5∶1. (C) Using passage 19 of (B) as the source, drug was administered at the dose of 2 000 mg/kg each week. The resistant index of passage 15 was 17\^41∶1. \{ Conclusion \} Line of P.berghei with medium level resistance to artemisinin was established.

OBJECTIVE:To investigate the dissolution of DEHP in PVC infusion set and the packaged infusion sets of the Paclitaxel injection so as to evaluate the safety of clinical infusion sets for paclitaxel.METHODS:The packaged infusion sets of Paclitaxel injection were collected from 9 Three-A grade hospitals in Chongqing.Paclitaxel injections were prepared as per actual concentration needed by the clinic and dripped through eight different infusion sets(in which 7 were packaged infusion sets) for 3 h.The peak area of the collected solution was measured and its content was determined by HPLC,and the effect of infusion time on the dissolution of DEHP was investigated as well.RESULTS:The dissolution of DEHP dripped through eight infusion sets were 1 408,9 393,6 576.5,2 412.6,8 194.4,0,8 477.2,and 8 037.4 ?g,respectively.The dissolution increased with the prolonging of infusion time.CONCLUSION:At present,the majority of packaged infusion sets of Paclitaxel injection were made form PVC materials.The safe medication of Paclitaxel can't be ensured for the dissolved DEHP can enter the blood directly,thus its hazardness should be given great attention.

Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Prognosis ; Staphylococcal Scalded Skin Syndrome ; diagnosis ; drug therapy

Objective To investigate the leukocyte infiltration inside the hematoma and the correlation between this and the expression of platelet P-selectin (CD62p) and platelet thrombin-sensitive protein (TSP) of patients with cerebral hemorrhage. Methods In 33 patients with cerebral hemorrhage treated with minimally invasive surgery, the expressions of platelet CD62p and TSP were detected by flow cytometry (FCM) and leukocyte counts were measured in intracerebral hematoma fluid and venous blood. The correlation analysis was performed between the two variables.Results The expressions of platelet CD62p and TSP in hematoma fluid were higher in patients with acute cerebral hemorrhage (20.25?1.82, 37.04?3.63) than those in venous blood (7.72?2.19, 12.32?2.81)(all P