1.Effects of PPAR-gamma agonist and MMP-2 on formation of atherosclerosis plaque in rabbits.
Feng LUO ; Zhao-hui WANG ; Lin-lin DU ; Jue WANG
Chinese Journal of Pathology 2007;36(8):556-557
Animals
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Aorta
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metabolism
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pathology
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Atherosclerosis
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etiology
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metabolism
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pathology
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Cholesterol, Dietary
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Male
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Matrix Metalloproteinase 2
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metabolism
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PPAR gamma
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agonists
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Rabbits
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Random Allocation
2.The application of Primovist in liver cirrhosis and small hepatocellular carcinoma
Lin LUO ; Hong SHAN ; Jin WANG
Chinese Journal of Hepatobiliary Surgery 2013;19(12):955-960
Primovist is a newly developed hepatocytespecific agent that is transported into the hepatocyte through organic anion transporting polypeptides (OATPs) and excre ted into bile through canalicular multiorganic anion trans porters.Primovist uptake is usually reduced in HCC cells that present hypointensity on the hepatobiliary phase,there fore this agent may help differentiate HCC from other neoplasms and improve histological grading.This article will further explore the use of Primovist in liver cirrhosis and small hepatocellular carcinoma.
3.Risk factors of vasovagal syncope
Ping LIN ; Haiyan LUO ; Cheng WANG
International Journal of Pediatrics 2011;38(6):535-537
Vasovagal syncope (VVS)is the most common clinical unexplained syncope,and its pathogenesis has not been fully understood yet.The contradictory reflection of cardiac autonomic nervous system caused by riggerring Bezold-Jarisch reflex,which could cause dilated blood vessels,blood pressure and cardiac output decreased,insufficient blood supply to the brain relate with syncope occurrence.Currently,most people consider it mainly relatel to the age and gender of patients,history of syncope,family history,mental and psychological factors,dietary habits and other factors,but the exact risk factors of VVS remains further study of multi-center and a large sample of data.
4.Determination of EDTA-2 Na in Amphotericin B by HPLC
Cheng WANG ; Ting LUO ; Ling LIN
China Pharmacist 2016;19(2):379-381
Objective:To establish an HPLC method for the determination of EDTA-2Na in amphotericin B. Methods: A Waters C18 column(50 mm × 4. 6mm, 5 μm) was used. The mobile phase A was acetic acid solution (1. 5 ml acetic acid was added into 1000ml water, and 41 ml 10% tetrabutylammonium hydroxide solution was added), and the mobile B was acetonitrile with gradient e-lution. The flow rate was 0. 8 ml·min-1 , the column temperature was 30℃, the detection wavelength was 260 nm and the injection volume was 25μl. Results:The results showed that EDTA-2Na in amphotericin B could be detected without any interference. The cal-ibration curve of EDTA-2Na was linear within the range of 0. 92-7. 37μg·ml-1(r=0. 999 9), the LOD was 1. 93 ng·ml-1 and the LOQ was 6. 45 ng·ml-1. The average recovery was 102. 5% (RSD=2. 8%, n=9). Conclusion: The method is simple, selective and accurate. It can be used for the quality control of EDTA-2Na in amphotericin B.
5.Promotive effect of basic fibroblast growth factor on cat corneal endothelial cell proliferation
Wenjuan LUO ; Jing LIN ; Chuanfu WANG
Chinese Journal of Tissue Engineering Research 2007;0(20):-
BACKGROUND:Previous experiments reported that basic fibroblast growth factor(bFGF) can change the shape of corneal endothelial cells,accelerate cell division and chemotaxis,and repair corneal endothelia cells injury in vivo and in vitro. OBJECTIVE:To investigate the effect of bFGF on cat corneal endothelial cells proliferation. DESIGN,TIME AND SETTING:The cell morphological observation was performed at the Central Laboratory of the Affiliated Hospital of Medical College of Qingdao University between January 2005 and December 2006. MATERIALS:Domestic cats were purchased from Experimental Animal Center of the Affiliated Hospital of Medical College of Qingdao University. METHODS:The corneal endothelial cells of cats were primarily cultured,and neurone specific enolase(NSE) immunocytochemistry staining was performed. After cell passage,bFGF(1,10,100 ?g/L) was added,continuously hatched for 1-5 days. MAIN OUTCOME MEASURES:MTT method was used to determine the proliferation of corneal endothelial cells;meantime,inverted phase contrast microscope and transmission electron microscope were used to observe the ultrastructure of cells. RESULTS:At days 1,3 and 5 after bFGF was added,the absorbance value in 490 nm was significantly higher than that of control group,especially in 10 ?g/L group. CONCLUSION:The bFGF can promote proliferation of cat corneal endothelial cells with effective concentration of 10 ?g/L .
6.Analyze the practice results of digital subtraction angiography composite operating room hundred grade purification
Minxin WANG ; Lijian LIN ; Jianrong LUO
Chongqing Medicine 2014;(9):1107-1109
Objective To discuss the practice effect about the waiting area in the hospital building of the daping hosptial ,the third military medical university to be reformed into digital subtraction angiography (DSA) composite operating room .Methods We reasonably choiced the form of purify conditioning ,medical C arm ,smallpox air supply ,and the efficient screening device layout at the end of smallpox air supply .Results The main parameters of the measured results met the national standards of indexes .The fan frequency was 48 Hz running at hundred grade ,and its operation energy consumption was about 25 kw/h ;the fan frequency was 35 Hz running at ten thousand grade ,and its operation energy consumption was about 12 kw/h;the conversion time was very short between hundred grade and ten thousand grade running ,about 10 minutes .Conclusion On the condition that the building environ-ment is not fit to install hanging medical C arm ,we can thorough choose floor type C arm ,adopt “leakage resistance type clean air supply smallpox” ,reasonably modify the form of purify air conditioning to design or modify a hundred grade operating room meet-ing requirement .
7.Selective transpedicular balloon kyphoplasty in treatment of osteoporotic thoracolumbar multi-vertebral compressive fractures:preliminary experience in 21 cases
Lin WANG ; Huiren TAO ; Zhuojing LUO
Orthopedic Journal of China 2006;0(04):-
[Objective]To evaluate the effect of selective kyphoplasty in treatment of osteoporotic thoracolumbar multi-vertebral compressive fractures based on preoperative magnetic resonance imaging(MRI).[Method]Twenty-one cases(fifty-seven vertebral bodies) of osteoporotic thoracolumbar multi-vertebral compressive fractures were treated from June 2003 to December 2006.The signal changes in different sequences were confirmed by preoperative MRI.Based on the MRI signal changes,57 vertebral bodies were treated by selective kyphoplasty.Normal balloon pressure and cement volume were performed for the painful vertebral bodies and lower pressure and cement volume were preferred for the vertebral bodies with old fracture.[Result]All patients tolerated the operation well with immediate relief of their back pain in 24 hours.The mean height of the anterior,media and posterior vertebral bodies were(1.7?0.9)cm,(1.7?0.7)cm,(2.5?0.9)cm preoperatively and(2.2?0.4)cm,(2.4?0.6)cm,(2.6?0.3)cm postoperatively.The mean kyphosis was improved from(33.7?10.9) degrees to(25.9?9.3) degrees(P
8.Determination of Cinobufagin and Resibufogenin in Qianglijiuxin Dripping Pills by HPLC
Xingping LUO ; Lanxia WANG ; Fen WANG ; Bochong ZHANG ; Ruichao LIN ;
Chinese Traditional Patent Medicine 1992;0(07):-
Objective: To determine the contents of cinobufagin and resibufogenin in Qianglijiuxin Dripping Pills. Methods: The HPLC method was used at Kromasil C 18 column of 250mm?4.6mm, 5?m, 0.5% Dipotassium Hydrogen Phosphate Acetonitrile (50∶50) as the mobile phase, 1.0mL/min as the flow rate at detection wavelength of 296nm. Results: For cinobufagin, the linear range was 0.67?g~4.7?g and the average recovery rate was 97.78% with RSD=1.2%. For resibufogenin, the linear range was 0.33?g~2.3?g and the average recovery was rate 99.61% with RSD=2.0%.Conclusion: The method can be applied to content determination of cinobafagin and Resibufogenin in Qianglijiuxin Dripping pills.
9.Comprehensive Healing Treatment of 30 Cases with Small and Short Penis in Simple Obesity Children
hong-ling, LI ; shi-lin, CHEN ; lin-lin, LI ; xiao-ping, LUO ; mu-ti, WANG
Journal of Applied Clinical Pediatrics 2004;0(07):-
Objective To investigate the therapy for small and short penis in simple obesity children.Methods Sixty simple obesity children with short and small penis were randomly divided into 2 groups(experiment group 30 cases and control group 30 cases).The experiment group received comprehensive treatment(consisting of massage of traditional Chinese medicine,behavior modification,dietetic and sport adjustment) to lose weight and treated by short and small penis healing apparatus.The control obesity children were given conducted behavior,dietetic and sport adjustment,and treated by short and small penis healing apparatus.Results After treatment,weight of 30 cases in the experiment group decreased,with a decrease of(7.75?3.50) kg.Body mass index(BMI) decreased from(31.10?3.88) before treatment to(27.82?3.49),and BMI before and after treatment changed significantly(t=12.68 P
10.Augmentation enterocystoplasty without reimplantation for patients with neurogenic bladder and vesicoureteral reflux
Deyi LUO ; Tongxin YANG ; Yifei LIN ; Kunjie WANG ; Hong SHEN
Chinese Journal of Urology 2015;36(2):104-107
Objective To assess the outcome of vesicoureteral reflux after augmentation cystoplasty in patients with neurogenic bladder.Methods Between January 2008 and January 2014,a total of 25 patients,with a hypocompliant bladder associated with vesicoureteral reflux confirmed by video-urodynamics preoperatively,were recruited in this study.They all had undergone bladder augmentation with a generous detubularized segment of bowel at our institution.No effort had been made to correct existing reflux.Preoperatively assessment included urinalysis,kidney function tests,ultrasonography,video-urodynamic evaluation.All patients had various degrees of vesicoureteral reflux.The status of vesicoureteral reflux and bladder function were studied by video-urodynamic.Results Mean follow-up was 2.2 years (range 0.5 to 5.5 years).The video-urodynamics manifested a significant improvement of bladder capacity,diminution of intravesical pressure and resolution of reflux after bladder augmentation.Of the 25 patients,20 (80%) no longer had reflux,3 (12%) had improvement,2 (8%) had no change.Sixteen of 18 with grades Ⅰ to Ⅲ (89%),all refluxing units with grade Ⅳ to grade Ⅴ (100%) showed complete cessation of reflux.Symptomatic urinary infection was not found after surgery.Conclusions Augmentation enterocystoplasty without ureteral reimplantation is effective and adequate for patients with high pressure and hypocompliant neurogenic bladder.Therefore,ureteral reimplantation is not necessary underwent when augmentation enterocystoplasty is recommended to patients with neurogenic bladder and vesicoureteral reflux.