Objective To observe the results of 45 cases with acquired immunodeficiency syndrome(AIDS) genotypic viral resistance test(GT) assay.Method The technitues provided by AB Applied Biosystem was used to check the mutations in the reverse transcriptase associated with significant viral resistance.Results Fourteen cases out of 40(35%)cases which failed from first line regimen showed resistance to both zedovudine(ZDV) and lamivudine(LMV),and 26 cases out of 40(65%) cases showed resistance to ZDV or lamivudine used in the first line.For no-nucleoside reverse transcriptase in habitor(NNRTIs),11 cases out of 40(27%) cases had resistance to nevirapine(NVP),29 cases out of 40(63%) cases showed resistance to both NVP and efairavir(EFV).Two cases out of 5 which failed from second line regimen showed that one drug from both first and second line had no resistance,so it still could be used in the new regimen.Conclusion GT assay is very useful for choosing a best and personalized regimen for AIDS patients.

BACKGROUND:Longitudinal study of changes in obesity is an important method to explore the etiology, which can provide scientific basis for preventing and control ing obesity.
OBJECTIVE:To investigate the effect of age, observation period and birth cohort on the obesity prevalence of adults in Jiangsu province through the age-period-cohort analysis.
METHODS:20-69-year-old adults in Jiangsu province were col ected as the research objects. The stratified cluster sampling method was used to col ect the obese data in 2000-2010, and analyzed with SAS software. RESULTS AND CONCLUSION:Obesity prevalence of 1946-1950 birth cohort to 1976-1980 birth cohort was gradual y increased (P<0.05) from 2000 to 2010. Obesity prevalence from 1931-1935 birth cohort to 1941-1945 birth cohort was not significantly increased (P>0.05). With the increasing age in each age group of over 25 years old, the risk of obesity was increased gradual y. There were significant differences in the odds ratios between the baseline groups of 20-25 years old and the age groups of over 25 years (P<0.05). Compared with the 1995-1999 birth cohort, the other periods had a slight influence on the obesity prevalence (P>0.05). Compared with 1976-1980 birth cohort, there were no significant differences in the risks of obesity of the birth cohorts after 1951-1955 (P>0.05). But the risk of obesity from 1946-1950 birth cohort to 1931-1935 birth cohort was gradual y significant since the 2000;obesity risks of those born in the different times were different;rural area wil be the key area than 45 years old.

BACKGROUND:Calcium phosphate cement and recombinant human bone morphogenetic protein-2 both have bone osteoinductivity. Maybe both of them could promote repair of tendon-to-bone interface damage. OBJECTIVE:To evaluate the effect of calcium phosphate cement-II containing recombinant human bone morphogenetic protein-2 on tendon-to-bone interface healing after rotator cuff injury . METHODS: Twenty-seven adult healthy rabbits were enroled in the study. The tendon-to-bone interface of the bilateral shoulder joints was taken from three rabbits as normal group, and the bilateral shoulder joints of the other 24 rabbits were subjected to acute rupture of the rotator cuff and tendon-to-bone reconstructive surgery as experimental group (n=12) and control group (n=12). Rabbits in the experimental group were treated with calcium phosphate cement-II, while those in the control group treated with nothing. Specimens were colected at 2, 4, 8 postoperative weeks for biomechanical test. RESULTS AND CONCLUSION:The maximum tensile strength of the rotator cuff in the experimental group was higher than that in the control group (P < 0.001), but lower than that in the normal group (P < 0.001). At postoperative 8 weeks, the rigidity of rotator cuff of the experiment group was higher than the control group (P < 0.001) but lower than the normal group (P < 0.001). These findings indicate that calcium phosphate cement-II can improve the maximum tensile strength and rigidity of rabbit tendon-to-bone interface in the early postoperative period, enhance tendon-to-bone interface binding force, and promote tendon-to-bone interface healing.

Objective To assess the feasibility of heterogeneous acellular dermal matrix(ADM)seeded with adipose derived stem cells(ADSC)for urethroplasty in a rabbit model.Methods ADSC were isolated from a rabbit and expanded in vitro,then identified by flow cytometry.We seeded ADSC onto the ADM and made it into tissue-engineered urethra.12 male rabbits were removed 1 cm urethra and divided into experiment group and control group.There were 6 rabbits in each group.Reconstructed urethra with tissueengineered urethra was used in experiment group,while unseeded ADM were used in control group.Urethrography was performed at 6 months after surgery.The animals were scarified at 3 and 6 months after surgery and the repaired urethra were harvested.H&E staining and immunohistochemical staining were performed with cytokeratin AE1/AE3 and smooth muscle desmin makers.Results The morphology of isolated ADSC was with long spindle cross-links,and had multicentral growth.Flow cytometry showed that the ADSC expressed CD166,CD105,CD90 and CD44,but not expressed CD45 and CD13.The cells could growth well on the ADM and showed good biocompatibility with it.All animals could void normally,urethrography showed there was no significant stenosis.3 months after surgery,the experiment group appeared regenerated smooth muscle but not in the control group,both groups did not regenerate urothelium.At 6 months urothelium and smooth muscle cells could be observed in the experiment group,but only the smooth muscle was evident in the control group.Conclusions By applying tissue engineering methods,we can seed the ADSC onto the heterogeneous ADM and make it into tissue-engineered urethra,which can help improve the reconstructive effect of urethra.

Animals ; DNA Methylation ; Female ; Genes, Tumor Suppressor ; Genes, ras ; Humans ; Lung Neoplasms ; genetics ; metabolism ; Nasopharyngeal Neoplasms ; genetics ; metabolism ; Signal Transduction ; Tumor Suppressor Proteins ; genetics ; metabolism ; Uterine Cervical Neoplasms ; genetics ; metabolism

OBJECTIVE:To standardized declaration of preparation registration in medical institutions. METHODS:With the combination of registration review of new varieties in the preparation registration in medical institutions in Liaoning province from 2010 to 2014,the existing problems in drug registration information of preparation in medical institutions were introduced,and the solutions and suggestions were proposed. RESULTS & CONCLUSIONS:The main problems of registration information of prepara-tion in medical institutions included non-standardized preparation name,unclear topic and writing,inappropriate instructions and la-bels,and imperfect preparation process,etc. To standardize the preparation registration authenticity and integrity of registration in-formation of medical institutions,it is suggested to increase on-site verification efforts,strengthen the five years clinical using his-torical inspection,improve the level of research and development of hospital preparations declaration units,and strengthen the idea that the medical institutions should be responsible for the quality and safety of preparations to ensure the safety and effectiveness.

Objective To investigate the value of retention rate of indocyanine green at fifteen minutes(ICGR15)during hemihepatectomy for evaluation of residual liver reserve function in patients with primary liver carcinoma.Methods During hemihepatectomy,ICGR15 was tested in 44 patients after the hepatic artery and portal vein of resected side were ligated.Child-Pugh score,Child-Pugh classification,and MELD score before operation were tested.After operation,the liver function condition was estimated.Results The incidence of liver dysfunction was significantly lower in ICGR150.05).ICGR15 and MELD score in normal liver function group were statistical lower than those in mild insufficiency of liver function group and severe insufficiency of liver function group(P

Objective : To investigate the clinical efficacy of prosthesis retained by metal clasp and flexible clasp in the repair of type Ⅴ maxillary defects after tumor resection. Methods: 23 maxillary tumor surgical patients were selected in this study and restored with maxillofacial prosthesis. Total 24 prosthesis combined with metal clasp and flexible dentures were followed up for half a year to 2 years. Results : After the application of prosthesis, better maxillofacial appearances are presented in all of the patients, Watian drinking water tests are improved to grade Ⅰ or Ⅱ from grade Ⅳ or Ⅴ in 21 patients, there was significant difference (P < 0.05); Soft diet can be masticated on affected side in 15 patients; The mean speech intelligibility score with and without prosthesis were (83.2 ± 7.3)% and (36.6 ± 5.6)%, there was significant difference (P < 0.05); Favorable hygienic situations of oral cavity and prosthesis are maintained in 18 patients. Conclusion Prosthesis combined with metal clasp and flexible denture performed favorable clinical efficacy and contributed to improve the living quality and mental health after tumor resection.

OBJECTIVETo compare the vibration parameters in reducible anterior disc displacement and symptomless youth, and explore the clinical diagnostic value of frequency spectrogram of temporomandibular joint (TMJ) sounds.

METHODS21 youth with anterior disc displacement were included as experimental group, and 26 symptomless youth were included as control group. The joint vibrations during rhythmic maximal open-close jaw movement of all subjects were recorded. The sensitivity and specificity of total integral in diagnosis of reducible anterior disc displacement were calculated.

RESULTSAll TMJ vibration parameters, include total integral, integral < 300 Hz, integral > 300 Hz, > 300/< 300 Hz radio, peak amplitude, peak frequency and median frequency, were significantly higher in experimental group than that in control group (P < 0.05). Along with the increasing of disc displacement degree, the TMJ vibration parameters were increasing, and total integral significantly increased (P < 0.05). The total integral demonstrated a high sensitivity and specificity in diagnosis of anterior disc displacement with reduction (0.86 and 0.85, respectively).

CONCLUSIONThe TMJ vibration parameters are significantly higher in youth with reducible anterior disc displacement than that in symptomless youth. Different pathological stages of disc displacement show different TMJ sounds.

Adolescent ; Female ; Humans ; Male ; Sensitivity and Specificity ; Sound ; Temporomandibular Joint ; Temporomandibular Joint Disc ; Temporomandibular Joint Disorders ; Vibration

AIM: To observe the expression change of growth and differentiation factor 10 ( GDF10 ) in the spinal cord of the rats with neuropathic pain .METHODS:Male SD rats (n=60) were used.The neuropathic pain was induced by ligation of left L 5 spinal nerves of the animals .The paw withdrawal threshold was detected 1 d before surgery , and 0 d, 1 d, 3 d, 10 d and 21 d after surgery.The changes of GDF10 in the dorsal horn of L5 spinal cord were detected by immunofluorescence staining and Western blot .RESULTS:The paw withdrawal threshold of the rats with spinal nerve ligation was decreased from 1 d after surgery until 3 d with obvious difference compared with the na ve rats ( P<0.05 ) , continuously decreased until 10 d, and then stabilized at 21 d.The GDF10 was located in the cytoplasm of the neurons in the dorsal horn of L5 spinal cord detected by immunofluorescence staining .The expression of GDF10 in L5 dorsal horn de-tected by immunofluorescence staining was reduced after surgery , significantly decreased from 10 d ( P<0.05) until more than 21 d after surgery in spinal nerve ligation group compared with na ve group.GDF10 in L5 spinal cord detected at 10 d after surgery by Western blot was significantly down-regulated in spinal nerve ligation group compared with na ve group (P<0.05).CONCLUSION:Spinal nerve ligation induces the decrease in GDF 10 expression in spinal dorsal horn .The down-regulation of GDF10 may contribute to the regulation of hyperpathia caused by mechanical stimulation after the injury of spinal nerve .