1.Methodology study of classification algorithm in traditional Chinese medicine syndrome study.
Journal of Integrative Medicine 2010;8(10):911-6
Study of traditional Chinese medicine (TCM) syndromes is a key to the research of TCM modernization, and the core is the classification and diagnostic criteria of syndromes. The purpose of this article is to review the usage of classification algorithms of data mining in TCM syndrome researches, and comprehensively analyze the main features of algorithms and their applications. The appropriate classification algorithm should be chosen according to different research purposes. Rough sets and cluster analysis are suitable for exploratory research without requiring a prior knowledge. Fuzzy sets theory, neural networks and decision tree are suitable for syndrome diagnostic criteria research when the classification goal is clear, because they require a prior knowledge. Among them, fuzzy sets theory could be used in combination with other classification algorithms. Thus, some new methods such as fuzzy clustering, fuzzy rough sets or fuzzy decision tree might be more suitable for TCM algorithm classification research. It is suggested that some novel classification algorithms need to be developed to fit the condition of TCM syndrome, based on the interdisciplinary theories and technologies.
2.Study on companion care needs of inlmtients at four tertiary hospitals in Wuhan
Xiuyun LI ; Min XU ; Jing WANG ; Yanxiang CHU
Chinese Journal of Hospital Administration 2012;28(7):519-522
Objective To investigate the companion care needs of inpatients at four tertiary hospitals in Wuhan,for providing targeted quality of care.Methods A questionnaire was customized according to Maslow's theory to survey 526 inpatients at four tertiary hospitals in Wuhan.Resnlts The companion care needs of inpatients were found high.Fore example,the esteem needs were the highest(2.41±0.39)yet with the lowest extent of satisfaction(2.59±0.43),followed by love and belonging needs(2.35±0.40)and safety needs(2.34±0.35)respectively.The physiological needs were the lowest(2.08±0.32)yet with the highest extent of satisfaction(2.63±0.44).Conclusion Nursing staff should change their mindset to respect patients' esteem needs,improve their nursing skills and service for safer care.Multimodel companion care needs should also be provided to meet patients' diversified needs.
3.Clinical study of autologous tumor tissue lysate loading dendritic cells for the treatment of hepatocellular carcinoma
Dongyin LI ; Chuan GU ; Jun MIN ; Zhonghua CHU ; Qingjia OU
Clinical Medicine of China 2008;24(7):693-696
Objective To investigate the feasibility and safety of autologous tumor tissue lysate loading den-dritic cells(DC) for the treatment of hepatocellular carcinoma (HCC). Methods The monocytes-derived DC were induced and antigen loaded with tumor tissue lysate to produce DC vaccine. Vaccination and clinical observation were conducted in 12 HCC patients for 41 times. Results The average output of DC was 1.69×107(1.69×107±9.44×106>) from 90 ml peripheral blood. 63.41% (26/41)patients appeared to develop delayed-type hypersensitivity after intradermal injection. After an average of 9 months follow up, 1 patient out of 4 recurrence and metastasis pa- tients survived for 17 months. The other three patients progressed. Out of 8 patients undergoing immunotherapy post- operatively,6 patients had no signs of recurrence and the others were found to have liver rceurrence and progression. Conclusion DC based immunotherapy is safe and feasible,with no side effects,which can be applied in the immu- notherapy strategy of HCC patients.
4.Effects of Tangxinping Capsule on angiotensin II and its type 1 receptor and myocardial ultrastructure in diabetic rats.
Min LI ; Qing NI ; Xiaoyan CHU ; Zhaoli WANG ; Lan LIN
Journal of Integrative Medicine 2008;6(11):1164-9
To investigate the protective effects of Tangxinping Capsule, a compound traditional Chinese herbal medicine, on diabetic cardiomyopathy in rats.
5.HPLC Fingerprints of volatile oil of Curcuma wenyujin in Zhejiang Province
Min LIU ; Shenghui CHU ; You CUI ; Xiaokun LI
Chinese Traditional and Herbal Drugs 1994;0(06):-
Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC.Methods The chromatographic fingerprint of C.wenyujin was established by HPLC with Shimadzu VP-ODS C18 column(250 mm?4.6 mm,5 ?m),acetonitrile-water(0.1 phosphoric acid) gradient elution,the flow rate of 1.0 mL/min,column temperatures of 30 ℃,and detective wavelength at 210 nm.ResultsTaking germacrone as the reference peak,14 common-peaks were selected as the fingerprint peaks.The similarities among samples of C.wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" published by GPC(Version 2004A) and cluster analysis.ConclusionThis method with better accuracy and reproducibility could be used in the quality control of C.wenyujin.
6.The maturation induction of human monocyte-derived dendritic cells
Dongyin LI ; Chuan GU ; Jun MIN ; Zhonghua CHU ; Qingjia OU
Chinese Journal of Immunology 1985;0(06):-
Objective:To investigate the most effective strategy for mature induction of dendritic cells.Methods:Human monocyte-derived dendritic cells were induced in the presence of cytokines GM-CSF and IL-4. On day 6, the immature DCs were pulsed with each of CD40L, LPS, TNF-? or a cocktail of cytokines(TNF-?, IL-6, IL-1?, PGE2). DCs were harvested after 24 h induction. The surface markers for maturation CD80,CD83,CD86 and HLA-DR were detected by FCM. FITC-Dextra endocytic activity was measured by FCM. IL-12 production was detected by ELISA. The capacity of DCs for T cell activation was detected by MTT assay.Results:CD40L,LPS,TNF-? and the cocktail of cytokines all could induce DCs’ maturation. The most effective scheme for induction of maturation was the cocktail of cytokines, and the expression rate of CD83 was up to 66.91%(P
7.Triceps Skinfold Thickness in Hemiplegics post Stroke in Community
Xiuling LIU ; Laiyong HOU ; Teng CHU ; Min LI
Chinese Journal of Rehabilitation Theory and Practice 2013;19(11):1052-1054
Objective To investigate the triceps skinfold thickness (TSF) of patients with stroke in community. Methods 42 hemiplegics after stroke and 42 normal persons in the same community were measured by a same doctor team, including height, weight, blood pressure, heart rate, waist circumference, hip circumference, and TSF. The grade of Brunnstrom and daily exercise time of the patients were investigated. Results The TSF was thicker in the non-affected side than in the affected side (P<0.05). The bilateral TSF was thinner in the patients than in the normal controls (P<0.05). In the patients, the TSF was thicker in those with function grade above Brunnstrom IV than those below (P<0.05), and in those who took part in exercise regularly than those did not (P<0.01). The body mass index was more in the patients than in the controls (P<0.05). Conclusion The TSF is thinner in the patients with stroke, which may associate with the motor function and daily exercise.
9.Detection and significance of phosphatidylinositol 3-kinase in adipose tissue of polycystic ovary syndrome patients with insulin resistance
Yong-Li CHU ; Hong-Yu QIU ; Yong-Yu SUN ; Min LI ; Hong-Fa LI ;
Chinese Journal of Obstetrics and Gynecology 2001;0(07):-
Objective To investigate the expression of phosphatidylinositol 3-kinase(PI-3K)in adipose tissue of polycystic ovary syndrome patients(PCOS),and explore molecular mechanisms of insulin resistance(IR)in PCOS.Methods Samples from patients with PCOS with IR(n=19),PCOS without IR (n=10)and controls(n=15)were collected.Serum fasting insulin(FIN)and fasting plasma glucose (FPG)were measure& Insulin resistance index was calculated using homeostasis model assessment (HOMA)to analyze the relationship between these markers and IR.Western blot technique was used to detect the PI-3K p85 subunit.Gene expression of PI-3K p85 subunit was detected by reverse transcription polymerase chain reaction(RT-PCR)method.Kinase activity was detected by immunoprecipitation,thin- layer chromatography and gamma scintillation counting.Results(1)The levels of FIN[(25.2?3.8) mU/L]and HOMA-IR(1.6?0.3)in PCOS with IR were significantly higher than those in PCOS without IR[(13.4 +3.8)mU/L,0.9?0.3]and controls[(9.5 +2.6)mU/L,0.5?0.3;all P<0.05).(2) There was no significant difference in the protein(0.65?0.10)and gene expression(0.92?0.12)of PI-3 K p85 subunit in PCOS with IR compared with PCOS without IR(0.72?0.10,1.01?0.10)and control groups(0.73?0.14,1.00?0.12;P>0.05).(3)PI-3K activity in PCOS with IR(81%)and PCOS without IR(89%)was significantly decreased(P<0.01,P<0.05)and negatively correlated with HOMA- IR(r=-0.69,P<0.01;r=-0.62,P<0.05).Conclusions No significant difference in the protein and gene expression of PI-3K p85 subunit in PCOS with IR is found.The decreased PI-3K activity may lead to IR of PCOS.
10.Spleno-left adrenal vein shunt for portal cavernous transformation
Zhengjun ZHANG ; Qimin CHEN ; Min XU ; Jun CHU ; Zhilong YAN ; Li HONG ; Song GU ; Ming HU
Chinese Journal of General Surgery 2010;25(1):17-19
Objective To evaluate the effect of spleno-left adrenal vein shunt for the treatment of portal hypertensive upper GI bleeding caused by portal vein cavernous transformation in children.Methods Spleno-left adrenal vein shunt was performed in 8 children with portal hypertension due to cavernous transformation.The clinical data was reviewed.Results Portal vein pressure decreased significantly from (30±11)mm Hg to(22±7) mm Hg after shunt.There was no mortality perioperatively and during the follow-up.There were no recurrent hemorrhage nor hepatic encephalopathy occurring in the follow-up and all the children have normal intelligence and normal liver function though blood ammonia level increased significantly from(18±7)μmol/L to (60±17)μmol/L in 4 cases.In 7 cases in which preoperative whole blood cell count significantly decreased,the postoperative WBC,RBC,Hb and PLT was (7.64 ±4.46)×10~9/L,(4.54±0.97)×10~(12)/L,(133±5) g/L and (355.40±107.36)×10~9/L respectively (all P <0.05).In one case suffering from preoperative low PLT count the postop PLT reached 333×10~9/L,which was significantly higher than that preoperatively.Esophageal varices ameliorated in 6 cases.No stenosis of anastomotic stoma and thrombosis developed.Conclusion Spleno-left adrenal vein shunt is an effective procedure to treat portal vein cavernous transformation induced portal hypertension in children.