Adolescent ; Asthma ; blood ; Bronchitis ; blood ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Uteroglobin ; blood

Agenesis of Corpus Callosum ; diagnostic imaging ; genetics ; pathology ; Brain ; diagnostic imaging ; pathology ; Chromosomes, Human, Pair 15 ; Humans ; Infant, Newborn ; Magnetic Resonance Angiography ; Male ; Radiography ; Translocation, Genetic ; genetics

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Neoplasms ; complications ; Respiratory Distress Syndrome, Adult ; complications ; Risk Factors

Aorta, Thoracic ; abnormalities ; Humans ; Infant ; Magnetic Resonance Angiography ; Male ; Pulmonary Artery ; abnormalities ; Recurrence ; Respiratory Insufficiency ; etiology ; Respiratory Sounds ; etiology ; Subclavian Artery ; abnormalities ; Vascular Diseases ; complications ; congenital ; diagnosis ; pathology ; physiopathology

Objective To explore the clinical significance of serum clara cell secretory protein(CC16),total immunoglobulin E(TIgE)and eosinophil cationic protein(ECP)in children with asthma.Methods Serum were collected from 59 cases during asthmatic acute attacks,29 asthmatic children who were in mild conditions,and 30 cases who were in moderate to severe conditions,and 30 healthy children.Serum CC16 concentration were measured by enzyme-linked immunosorbent assay(ELISA),TIgE and ECP concentration were measured by uniCAP100.Results The levels of CC16 in serum of asthmatic children during acute attacks were significantly lower than that in control group(t=2.93 Pa

Objective To explore the expression of GATA-3 in pulmonary tissue of asthmatic mice and the inhibitory effect of dexamethsone(Dex)on it.Methods The Blab/c mice asthma model was induced by ovalbumin(OVA) with classic method.Twenty-four male mice were randomly divided into control group,asthmatic group and Dex treated group.The expression of GATA-3 protein was measured by immunohistochemical staining.The expression of GATA-3 mRNA was measured by reverse transcription-polymerase chain reaction(RT-PCR).The level of IL-4 in mice spleen CD4 T cell was measured by flow cytometry.The airway inflammation was evaluated by HE staining.Results The percentages of postive GATA-3,GATA-3 mRNA and IL-4 protein of asthmatic group were significantly higher than those of control group(P

Objective To investigate the changes of plasma activated protein C(APC)in the pediatric leukemia patients with acute lung injury(ALI),and the relationship with the outcome.Methods During the study period(from Jan to Dec 2009),17 pediatric leukemia patients with ALI were selected.They were divided into neutropenic(n =10)and non-neutropenic(n =7)group.We collected the basic data including the age,gender,stage of chemotherapy,pediatric critical illness score,mechanical ventilation time,ICU time.The blood gas and plasma APC levels were detected on day 1 and day 4 of ALI onset.Results Compared with the non-neutropenic group,the neutropenic group had a significantly longer mechanical ventilation time [(16.60 ± 1 0.83)d vs(3.79 ± 4.08)d,P =0.009 6],lower PaO2 level on day l[(54.90 ± 17.05)mm Hg vs (92.70 ± 27.53)mm Hg,P =0.009 7],and lower APC level on day 4[(193.06 ± 63.19)pg/mlvs(286.28 ±25.12)pg/ml,P =0.007 7].Conclusion The APC generation is severely impaired in the neutropenic group,especially in those who died.The lower APC level is,the longer is the mechanical ventilation time,the worse is oxygenation,the poorer is the prognosis.APC replacement therapy may be promising in these patients.

Objective To compare the clinical features, diagnosis, treatment and prognosis between macrophages activation syndrome (MAS) and other hemophagocytic syndrome (HPS). Methods Thirty-six children with HPS were identified at Nanjing Children's Hospital during January 2006 to March 2009. They could be classi-fied into MAS group (13 patients) and other HPS group (23 patients). All relevant clinical features, laboratory data, treatments and outcomes were analyzed with t test,χ2 test and Fisher's exact test.Results Patients with MAS tended to be elder than those with other HPSs [(7.7±1.3) years vs (2.6±0.5)years, t=3.899, P=0.004]. There was no difference in gender distribution. In MAS cases, the central nervous system (69% vs 13%, P=0.001), circulatory system (23% vs 9%, P=0.047) and the urinary system (38% vs 9%, P=0.033) were usually involved. The clinical symptoms of MAS were more sever than other HPS. Serum ferritin [(9703±9819) μg/L vs (4569±1396) μg/L, t=2.854, P=0.015] and erythrocyte sedimentation rate (ESR) [(53±32) mm/1 h vs (20±14) mm/1 h, t=2.708, P=0.020] changed more obviously in MAS cases compared with other HPS. Conclusion, Childhood MAS is different from other HPS in terms of age, etiology, clinical manifestations, laboratory tests and treatments.

Objective To assess the in vitro affinity and apoptosis-inducing effect of 131I-K237 peptide (H-His-Thr-Met-Tyr-Tyr-His-His-Tyr-Gln-His-His-Leu-OH) to LNCaP prostate cancer cell line.Methods The K237 peptide was radiolabeled with 131I by the Iodogen method.The radiolabeling efficiency and radiochemical purity after purification were then characterized by TLC in vitro.LNCaP cells were inoculated in 96-well cell plate and divided into following groups (3 duplicate wells for each group):15 kBq 131I-K237was added in the experimental group,different doses of Na131I (5,10,15 kBq) were added in 3 negative control groups,15 kBq 131I-K237 with different doses of unlabeled K237 (1,2,4,8,16 μ g/μl) were added in 3 blocking groups,and PBS was added in blank control group.The cellular binding ratios were calculated after 48 h.LNCaP cells were inoculated in 24-well cell plate and divided into 3 groups:131I-K237group,which including 3 different dose subgroups (5,10,15 kBq) ; unlabeled K237 group,which including 3 different dose subgroups (1,2,4 μg/μl) ; blank control group with 100 μl PBS.All the cells were cultured for 48 h,then optical microscopy (OM) and fluorescence microscopy (FM) were used to observe the cell morphology ; DNA gel electrophoresis was conducted and flow cytometry (FCM) was used to estimate the apoptotic rate of LNCaP cells.One-way analysis of variance and the least significant difference (LSD)-t test were used to analyze the data.Results The labeling efficiency of 131I-K237 was (73.7±3.2) ％ and the radiochemical purity was (96.7±0.6) ％ after purification.The binding ratio of experimental group was (95.8±1.5)％,whereas the ratio of negative groups with 5,10,15 kBq Na131I and PBS group was (8.2±0.4) ％,(8.3±0.6) ％,(8.6±0.5) ％ and 0,respectively.The binding ratio of 131I-K237 and LNCaP significantly declined with the increased dose of unlabeled K237 (t=4.71,P＜0.01).The apoptosis of LNCaP cells cultured with 131I-K237 was observed.Typical DNA ladder was found by DNA gel electrophoresis.The apoptotic rates of 5,10,15 kBq131I-K237 groups were (34.1±2.9)％,(37.3±3.4)％ and (41.7±3.6)％,respectively; whereas those of unlabeled K237 groups and blank control group were (10.8±1.0) ％,(12.5±2.1) ％,(13.1±2.4) ％ and (2.9±0.3) ％,respectively.There were significant differences of apoptotic rate among groups (F=76.31,P＜0.05).The difference among 5,10,15 kBq 131I-K237 groups was statistically significant (t=3.09,3.27,4.52,all P＜0.05).Conclusion 131I-K237 can bind to LNCaP cells with highly affinity and has significant apoptosis-inducing efficacy on the prostate cancer cell line.

Objective To study the clinical and pathological influence of high uric acid on idiopathic membranous nephropathy (IMN) Methods A retrospective study with 314 patients diagnosed with IMN from January 2014 to June 2016 was conducted and the clinical pathological influence of high uric acid on IMN was analyzed.Results (1) Of the total,the prevalence of hyperuricaemia patients was 23.2％ (73 cases);(2) The difference of age,course of the disease,blood pressure and symptoms between hyperuricaemia IMN patients and IMN patients with normal uric acid was statistically significant (P ＜ 0.05);(3) Laboratory test indexes such as blood lipid and renal function between hyperuricaemia IMN patients and IMN patients with normal uric acid indicated statistical significance (P ＜ 0.05);(4) The pathological damage was aggravated in hyperuricaemia IMN patients (P ＜ 0.05).Conclusion High uric acid can enhance the clinical and pathological damage of IMN,and comprehensive and effective treatments should be conducted to delay the development of disease.