3.Radiobiological effects of irradiation plus olaparib with different dose rates on CNE-2 cell line
Gang ZHAO ; Xiu HUANG ; Taihao ZHENG ; Xuejun SHI ; Meng LI ; Ming LI
Chinese Journal of Radiation Oncology 2016;25(7):764-769
Objective To investigate the radiobiological effects of radiation with different dose rates on human nasopharyngeal carcinoma cell line CNE-2 treated with or without a poly ADP-ribose polymerase (PARP) inhibitor,olaparib.Methods The concentration of olaparib used to treat cells equaled to the inhibition concentration IC10 of olaparib to CNE-2 cells.The CNE-2 cells were divided into acute radiotherapy (RT) group,fractionated radiotherapy (FRT) group,olaparib + RT group,and olaparib + FRT group.All groups were exposed to radiation of 0,1,2,3,5,7,and 10 Gy at a dose rate of 3 Gy/min.The delivery time for each dose point was 4 min in RT and 30 min in FRT.The colony forming assay was used to evaluate the survival of CNE-2 cells at each dose point.The multi-target,single-hit model was used to fit the cell survival curves and the parameters,D0,Dq,and SF2,were calculated.At dose points of 0.1,and 2 Gy,western blot was used to determine the expression of PARP-1 in the RT group and the FRT group and γH2AX in each group.Immunofluorescence was used to evaluate the γH2AX focus formation.A single factor analysis of variance was used to compare the 4 groups,and two two compared with SNK-q test.Results The IC10 value of olaparib to CNE-2 cells was 4.0 μmoL/L.At dose points of 1 and 2 Gy,the PARP-1 expression was significantly higher in the FRT group than in the RT group (P=0.029,0.022),while the γH2AX focus number was significantly smaller in the FRT group than in the other three groups (all P<0.05);compared with the RT group,the D0,Dq,and SF2 values in the FRT group were increased by 11.67%,15.78%,and 23.61%,respectively;compared with the FRT group,the D0,Dq,and SF2 values in the Olaparib+ FRT group decreased by 11.19%,6.44%,and 13.26%,respectively;there were no significant differences in above indices between the RT group,the Olaparib+RT group,and the Olaparib+FRT group.Conclusions For the same radiation dose,fractionation reduces the relative dose rate and weakens the radiobiological effects.lowdose olaparib can compromise the single strand break repair induced by the decline of the relative dose rate in a fractionated irradiation mode,which promotes the formation of double-strand break and improves the radiobiological effects.
4.Effect of Xinfeng Capsule on Lipoprotein Metabolism of Rheumatoid Arthritis Patients.
Jian LIU ; Lei WAN ; Chuan-bing HUANG ; Yuan WANG ; Yu-zhen PAN ; Xiu-li XIE
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(9):1060-1064
OBJECTIVETo explore the effect of Xinfeng Capsule (XC) on lipoprotein metabolism of rheumatoid arthritis (RA) patients.
METHODSTotally 180 RA patients were assigned to the experimental group and the control group by random digit table, 90 in each group. Patients in the experimental group took XC (three pills each time, three times daily), while those in the control group took Methotrexate Tablet (four tablets each time, once per week). One month consisted of one therapeutic course and all patients were treated for two therapeutic courses. A healthy control group consisting of 60 patients was also set up. Changes of lipoprotein indices, clinical efficacy, lipid metabolism, joint symptoms and signs, activity indicators were observed, and correlation analyses were performed.
RESULTSCompared with the healthy control group, expression levels of prealbumin (PA), globulin (GLO), high-density lipoprotein (HDL), apolipoprotein Al (Apo-A1) were lowered in RA patients (P <0. 05, P <0. 01). Correlation analyses showed that PA was negatively correlated with joint tenderness, morning stiffness time, disease activity score (DAS-28), C-reactive protein (CRP), interleukin (IL)-6, respectively. Total protein (TP) was negatively correlated with joint tenderness. GLO was negatively correlated with joint tenderness and DAS-28. HDL was negatively correlated with erythrocyte sedimentation rate (ESR) and endothelin (ET)-1. Apo-Al was negatively correlated with joint pain; Apo-B was negatively correlated with CRP; LDL was negatively correlated with morning stiffness time (P <0. 05, P <0. 01). Compared with before treatment, expression levels of PA, HDL, Apo-A1 , Apo-B, and serum IL-10 contents increased, and expression levels of ESR, CRP, IL-6, ET-1 , joint pain, joint swelling, morning stiffness time, and DAS-28 decreased in the experimental group (P <0. 05, P <0. 01). PA increased more after treatment than before treatment in the control group (P <0. 01). There was statistical difference in joint symptoms (except joint tenderness) and activity indices (except ET-1) in the control group (P <0. 05, P <0. 01). Compared with the control group after treatment, PA and HDL increased, ET-1 and duration of morning stiffness decreased in the experimental group (all P <0. 05).
CONCLUSIONSLipoprotein metabolic disorder exists in RA patients, and it is associated with disease activity. XC could obviously improve lipoprotein metabolism and joint symptoms.
Arthritis, Rheumatoid ; drug therapy ; metabolism ; Blood Sedimentation ; C-Reactive Protein ; Capsules ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Humans ; Interleukin-10 ; Interleukin-6 ; Lipoproteins ; Lipoproteins, HDL ; metabolism ; Methotrexate
5.Effect of Compound Shi-Chang-Pu Granula on Behavior, Structure of Hippocampus in Epileptic Mice Induced by Pentylenetetrazol
Xiuju LI ; Qiaoling HE ; Xiu LUO ; Jianjun MING ; Renbin HUANG ; Shijun ZHANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(5):1014-1019
This study was aimed to observe the effect of Compound Shi-Chang-Pu (CSCP) granula on behavior, structure changes of hippocampus with epileptic seizure of mice induced by pentyenetetrazole (PTZ). Sixty Kunming mice, which were half male and half female, were randomly divided into 6 groups, which were the blank control group, animal model, phenytoin (PHT) group, high-dose CSCP (4 000 mg·kg-1) group, middle-dose CSCP (2 000 mg·kg-1) group and low-dose CSCP (1 000 mg·kg-1) groups. The blank group and model group were given intragas-tric administration the same volume of saline, the others animals treated with continuous oral administration of drugs for 7 days. Intraperitoneal injection of PTZ (80 mg·kg-1) was given 1 h after the last medication to establish the a-cute epilepsy model (expect the blank control group). Observation was made on the influence of CSCP on incubation period, behavior degree and times of epileptic seizure among PTZ induced epilepsy mice, the structure changes of mice were detected by the brain tissue HE staining. The results showed that CSCP can improve the degree of seizure attack, prolong the incubation period of mild attack, and decrease Ⅳ and Ⅴ degree epileptic seizure, the PTZ in-duced epilepsy mice could damage the structure of hippocampus, reduce the number of cells, PHT and CSCP could ameliorate these changes. It was concluded that CSCP had certain inhibition on epileptic seizure mice induce by PTZ and ameliorate the damage of hippocampus. The antiepileptic mechanism still requires futher study.
6.Different metabolites of leaves between Tripterygium wilfordii and Tripterygium hypoglaucum based on UPLC-Q-TOF-MS.
Chao LIU ; Qing-xiu HAO ; Yan JIN ; Lu-qi HUANG ; Li-ping KANG ; Lan-ping GUO
China Journal of Chinese Materia Medica 2015;40(9):1710-1717
To analysis the differences between Tripterygium wilfordii and T. hypoglaucum, specimens of their leaves were collected from five production regions and analyzed by ultra performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS). The data were analyzed by multivariate statistical method, such as hierarchical cluster analysis (HCA) principal component analysis (PCA) and orthogonal signal correction partial least square discrimination (OPLS-DA). Potential markers with VIP values above 5.0 and corresponding r values above 0.85, were selected and further tested by combining mann-Whitney nonparametric. Those with P < 0.001 and AUC = 1 were confirmed as metabolite markers to discriminate them from each other. Results revealed that the two species were obviously different in their leaf metabolites. Based on their mass spectra, 23 potential metabolite markers were identified to distinguish T. wilfordii from T. hypoglaucum.
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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chemistry
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metabolism
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Mass Spectrometry
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Molecular Structure
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Plant Leaves
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chemistry
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metabolism
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Tripterygium
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chemistry
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classification
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metabolism
7.Observation on ocular damage in trichloroethylene-induced medicamentosa like dermatitis.
Xiu-qin WANG ; Wei-xin HUANG ; Li-hua XIA ; Jia-bin CHEN ; Ying ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(2):116-117
Adolescent
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Adult
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Dermatitis, Occupational
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diagnosis
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Eye Diseases
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diagnosis
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etiology
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Female
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Humans
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Male
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Trichloroethylene
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poisoning
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Young Adult
8.Clinical Significance of Monitoring Indexes of Oxygen Exchange in Neonatal Respiratory Distress Syndrome
yu-dan, HUANG ; er-li, QU ; xiu-rui, DENG ; qing, CHEN ; wei-dong, SU
Journal of Applied Clinical Pediatrics 1992;0(06):-
0.05).Conclusion Indexes of oxygen function may become criteria of early diagnosing NRDS,observing effect of treatment and guidance of ventilation weaning.
9.Design, synthesis and antiproliferative activity in cancer cells of novel 18β-glycyrrhetinic acid derivatives.
Min HUANG ; Kun LI ; Shu-yu JIN ; Ting-xiu CUI ; Dan LIU ; Lin-xiang ZHAO
Acta Pharmaceutica Sinica 2015;50(10):1263-1271
To investigate the anticancer effects of ring C in 18β-glycyrrhetinic acid (GA), a series of GA derivatives featured with 9(11)-ene moiety in ring C were designed and synthesized. The structures were confirmed by IR, LC-MS and 1H NMR. Their inhibitory effects towards human prostate cancer PC-3 and leukemia HL-60 cell lines were determined. Most of the derivatives displayed stronger antiproliferative activities than GA. Particularly, compound 14 showed promising anticancer activity with the GI50 values of 4.48 µmol · L(-1) and 1.2 µmol · L(-1) against PC-3 and HL-60 cells respectively, which is worth further study.
Antineoplastic Agents
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chemistry
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pharmacology
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Cell Line, Tumor
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drug effects
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Cell Proliferation
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drug effects
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Drug Design
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Glycyrrhetinic Acid
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analogs & derivatives
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chemistry
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HL-60 Cells
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drug effects
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Humans
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Male
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Prostatic Neoplasms
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pathology
10.Evalution of the effect of carvedilol on left ventricular function by Doppler ultrasound MPI in patients with heart failure
Guiying CHEN ; Lijun ZHOU ; Xianyou JI ; Weimin LI ; Chunhong XIU ; Yonglin HUANG
Chinese Journal of Practical Internal Medicine 2001;0(03):-
Objective To evaluate the effects of carvedilol on left ventricular function by MPI in patients with heart failure.Methods All the cases came from the first Affiliated Hospital of Harbin Medical University during 2004 to 2006.There were two groups:45 Carvedilol treated patients and 50 placebo controlled patients.The patients with heart failure were detected the conventional Tei index(c-Tei),the tissue Doppler Tei index(t-Tei)and left ventricular ejection fraction pretherapy and post-treatment 1 month and 6 month.Results The patients with heart failure were higher in LVEDd,c-Tei,t-Tei and lower in LVEF compared with the controlled patients(P