Recent work has revealed that large numbers of mitotic figures are present in adult hearts,which has implied that there should be some mechanism about nature repair of the adult heart.But the levels of natural repair are not high enough to retrieval the traumatic myocardium.As a new approach to improving the cardiac repair,stem-cell therapy provides an exciting and powerful treatment.We describe the background of natural repair,and summarize the current strategies in the application of stem-cell therapy to repair the traumatic myocardium.

Objective To evaluate the short-term effects of three-dimensional conformal radiotherapy (3D-CRT) associated with transcatheter arterial chemoembolization (TACE) in the treatment of primary hepatic carcinoma and 3D-CRT associated with chemotherapy in the treatment of metastatic hepatic carcinoma. Methods In 52 patients with primary hepatic carcinoma, first of all a single TACE was conducted. Then after an interval of 2 weeks, the conformal radiotherapy was given, with a radiation dose of 2~3 Gy every fraction, once daily or once every other day and 3~5 fractions every week. The total radiation dose to the target was 42.2~60.0 Gy (mean, 52.2 Gy). Afterwards, 2 additional TACEs were given after the radiotherapy. In 35 patients with metastatic hepatic carcinoma, chemotherapy was carried out for 1 treatment course firstly. Then 3D-CRT was employed. After the radiotherapy, another 2 courses of consolidating chemotherapy were administrated. The patient continued with the chemotherapy for additional 3 treatment courses (a total of 6 courses) if efficacy evaluation showed the patient had responded to the chemotherapy. Results The response outcomes were: complete remission (CR) 23 0% (20/87), partial remission (PR) 39 1% (34/87), stable disease (SD) 34 5% (30/87) and progressive disease (PD) 3 4% (3/87), the response rate (RR) being 62 1% (54/87). Conclusions 3D-CRT with TACE for primary hepatic carcinoma and 3D-CRT with chemotherapy for metastatic hepatic carcinoma offers high local control rates and good short-term effects.

Objective To study the inhibition activity of volatile oil from Mappianthus iodoies on SPC-A-1 and BEL-7402 cancer cells.Methods The volatile oil in Mappianthus iodoie was extracted by SFE-CO2.MTT assay was employed to test the antitumor effect of volatile oil from Mappianthus iodoies in two kinds of malignant tumor cell lines,with IC50 applied to evaluate the degree of inhibition activity.Results When the dose of volatile oil from Mappianthus iodoies was 200 μg/ml,the inhibition ratios of the tumor cell was in excess of 50％,the IC50 was 169.54,695.21 μg/ml respectively.Conclusion Volatile oil from Mappianthus iodoies extracted by SFE-CO2 has obvious inhibition activity on SPC-A-1 and BEL-7402 cancer cells.

Objective The effect of intermittent iron supplementation weekly and twice weekly was studied in children with iron deficiency anemia(IDA). Methods Subjects were 58 children who were randomly divided into two groups. One group received a dosage of 2mg/kg Fe every 3d for 9 weeks. The other group received the same dose Fe once a week for 12 weeks. Results Hemoglobin and serum ferritin increased significantly after treatment in both groups (P ＜0.05), and zinc protoporphyrin decreased significantly (P ＜0. 05). But serum ferritin of both groups was different after 6 weeks of treatment (P ＜0. 01). The side effect of the group supplemented once every 3d was higher than that of the group once a week, but there was no statistically significant difference. Conclusion Iron sup plementation every 3d has a similar effect to once a week for treatment of IDA. The former should be used for the se rious patients for 6 weeks. The later should be used for infants and the patients whose resistance of intestines and stomach are not good.

ObjectiveTo explore the mechanism and prevention for the hypertension complicated by the retroperinoneal paraganglioma operation.MethodsBlood pressures were monitored during operations on 15 patients with retroperinoneal paraganglioma. Also,the reason and the prevention for the changed blood pressure were studied emphatically during operation.ResultsThe elevated blood pressure of 12 patients was detected during operation,and all dropped to a varied degree after operation,with 2 of them below the normal value.ConclusionThe retroperinoneal paraganglioma operation was liable to cause blood pressure elevated,which was related to the anesthesia induction and the operation stimulation of tumor. After the excision of the tumor,blood pressure of most of the patients dropped to some degree. Therefore,during the retroperinoneal paraganglioma operation,the blood pressure should be monitored closely,and the short-acting antihypertensives should be applied on time. Meantime,occurrence of hypotension was also considered after the excision of the tumor.

Objective To investigate the influence of gender differences on NF-kB activation in livers in septic rats. Methods Total 20 female and 20 male Wistar rats were randomly divided into four groups.Tissue samples of the livers were collected to measure NF-kB activation by EMSA.The level of plasma ALT,TNF-?and estrogen were measured also. Results NF-kB activation in normal male and female rats has no significant difference (P＞0.05).After stimulated by LPS,the level of NF-kB activation and ALT,TNF-?in plasma were markedly upregulated,and the index of female group lower than that in male group (P＜0.01).The level of NF-kB activation in livers and ALT,TNF-?in plasma both in male and female have significantly negative correlation with the level of estrogen in plasma (P＜0.05 ).Conclusion There are significantly gender differences on NF-kB activation in livers in septic rats.Estrogen may decrease the injury of livers in septic rats.

OBJECTIVETo compare the therapeutic effect of Astragalus and Angelica Mixture (AAM) on treating CKD patients according to different CKD primary diseases, staging and TCM syndromes.

METHODSA multicentre, open-label, and self control clinical design was used, and thirty-two patients in line with inclusive criteria were recruited. Based on maintaining their previous basic CKD treatment, patients additionally took AAM (Astragalus and Angelica each 30 g), once a day, three months consisted of one therapeutic course. Serum creatinine (SCr), estimated glomerular filtration rate (eG- FR), 24 h urinary total protein (UTP), plasma albumin (ALB), hemoglobin (Hb), and changes of TCM syndrome factor integrals were compared before treatment, at the end of month 1, 2, and 3. The differences in the aforesaid indices were compared between CKD patients with different CKD primary diseases (chronic glomerulonephritis, chronic renal tubulointerstitial disease, hypertensive renal damage), different CKD stages (CKD 3 and CKD 4), and patients of qi-blood deficiency syndrome (QBDS) and non-QBDS.

RESULTSAAM could improve 78.12% (25/32) patients' renal function. Compared with before treatment, SCr decreased (12.08% +/- 10.11%), eGFR increased (21.14% +/- 18.55%), and ALB increased (2.76% +/- 1.97%) at the end of 3-month treatment (all P < 0.05). As for TCM syndrome factor integrals, compared with before treatment, the integrals for qi deficiency syndrome, blood deficiency syndrome, and yin deficiency syndrome decreased, while the integrals for dampness heat syndrome and turbid-toxin syndrome increased (all P < 0.05). There was no obvious difference in all indices except the integral for hypertensive renal damage patients of yin deficiency syndrome (P > 0.05). The SCr decreasing percent was 19.82% +/- 8.30% for patients of non-QBDS and 5.24% +/- 10.75% for patients of QBDS. The latter was higher with statistical difference (P < 0.05). As for TCM syndrome factor integrals, the integral differences of qi deficiency and blood deficiency were obviously higher in patients of QBDS, when compared with patients of non-QBDS (P < 0.05).

CONCLUSIONAAM could improve the renal function of CKD patients, elevate their ALB levels, and ameliorate associated qi deficiency syndrome, blood deficiency syndrome, and yin deficiency syndrome, especially for CKD patients of QBDS.

Adolescent ; Adult ; Aged ; Angelica ; Astragalus Plant ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Renal Insufficiency, Chronic ; drug therapy ; Treatment Outcome ; Yin Deficiency ; drug therapy ; Young Adult

Adenocarcinoma, Clear Cell ; metabolism ; pathology ; surgery ; Adult ; Carcinoma ; metabolism ; pathology ; Carcinoma, Mucoepidermoid ; metabolism ; pathology ; Carcinoma, Renal Cell ; metabolism ; pathology ; secondary ; Diagnosis, Differential ; Humans ; Keratins ; metabolism ; Male ; Nasal Cavity ; Nose Neoplasms ; metabolism ; pathology ; surgery ; S100 Proteins ; metabolism

Objective To obtain the experimental data of vascular tissue engineering.MethodsThe vascular endothelial cells (VEC) and vascular smooth muscle cells (VSMCs) were acquired and cultured, and then seeded on vascular tissue engineering materials. The porous gelatin-chitosan scaffold with VSMCs was subcutaneously implanted, followed by the observation of the cell growth ten days later.ResultsThe two kinds of cells were successfully cultured and their morpholoical and immunohistochemical characteristics were consistent with vascular endothelial and VSMCs respectively. The VSMCs could grow extensively on the scaffold after the in vivo implantation. The scaffold were wrapped by the fibrous tissue ten days later after the in vitro implantation of VSMCs. The seed cells grew in the scaffold, and the vessel cavity seen in the center of the scaffold, was quite different from the normal vessel structure.ConclusionIt is feasible to implant the VSMCs with fibrin gels into the living body. The vessels reconstructed, though different from the normal structure, is similar to the embryo of the vessels.

ObjectiveTo investigate the feasibility of construction of engineered blood vessel using chitosan tube and fibrin gel as scaffold.MethodsVascular endothelial cells and smooth muscle cells were harvested from aortas of a rat, respectively. After expansion in vitro, vascular endothelial cells were seeded onto the inner surface of chitosan tube and smooth muscle cells mixed with fibrin gel seeded onto outer surface of the scaffold to construct engineered blood vessels. Inverted microscope, immunohistochemical staining and scanning electronic microscope were used to evaluate the construct.ResultsVascular endothelial cells formed monolayer and covered the inner surface of chitosan tube. Smooth muscle cells survived in the fibrin gel and grew in a 3-dimensional manner. ConclusionChitosan-fibrin gel may be potentially used as scaffold of engineered blood vessels.