Objective To investigate the relationship between the hepatic membrane protein MRP3(multidrug resistance-associated protein 3,MRP3) expression and the nuclear hormone receptor RXR?(retinoid-X receptor alpha,RXR?)expression in both cultured hepatoma cell HepG2 and bile duct ligated(BDL) rat liver.Methods Total cellular protein and nuclear protein were isolated from HepG2 cells induced by chenodeoxycholic acid(CDCA) or phenobarbital(PB),as well as from the liver tissue of BDL rats.The protein expressions of MRP3 and RXR? were determined by Western blotting.Results The membrane protein MRP3 expression was significantly enhanced and the nuclear receptor RXR? expression was suppressed by CDCA or PB in HepG2 cells.Similarly up-regulation of MRP3 and down-regulation of RXR? were also observed in BDL rat liver.Conclusion The up-regulation of hepatic membrane protein MRP3 may be associated with down-regulation of nuclear receptor RXR?.

Objective To explore the level of hypoxia-inducible factor-1α( HIF-1α) and vascular endotheli-al growth factor( VEGF) in serum and tissue of patients with metrocarcinoma.Methods 48 patients diagnosed with cervical cancer were analyzed retrospectively.Patients were treated with paclitaxel combination chemotherapy,HIF-1αand VEGF levels in serum of patients were detected by ELISA method before and after the treatment.HIF-1αand VEGF levels in cervical tissue of patients were observed by immunehistochemical staining methods.Western blot method was used to detect the change of HIF-1αand VEGF expression levels in cervical tissues of patients.And the levels of HIF-1αand VEGF in patients with lymphatic metastasis and lymph node metastases were compared. Results Lymph node metastasis did not occur in patients with uterine cancer serum HIF-1αand VEGF were (16.71 ±34.75)ng/L,(255.77 ±87.99)ng/L,respectively,and those after treatment were (98.33 ±32.17)ng/L, (153.67 ±65.45)ng/L,the differences were statistically significant(t=9.416,5.001,P=0.003,0.007) before and after treatment;The serum of patients with lymph node metastasis of uterine cancer in HIF-1αand VEGF increased, and cervical cancer patients after treatment,cervical tissue HIF-1αand VEGF;The metastasis were statistically signif-icant differences occurred(t=3.055,16.196,P=0.038,0.000);HIF-1αand VEGF decreased after treatment,the expression levels were significantly decreased compared with before treatment ( t=5.899,P=0.004;t=9.649,P=0.001);patients with lymph node metastasis after treatment,the expression levels of HIF-1αdecreased compared with before treatmen ( t =5.233, P =0.004), but there was no significant changes in the expression of VEGF. Conclusion The mechanism of taxol anti-uterine cancer may be related with inhibition the expression of HIF-1αand VEGF protein,and thereby inhibiting tumor angiogenesis and cancer metastasis.

Neonal necrotizing enterocolitis (NEC) is the most common gastro-intestinal emergency occurring in neonates and it can lead to death of neonates and low birth weight infants.Although numerous risk factors have been implicated in the pathogenesis of NEC,the etiology of the disease remains unclear.It lacks effective methods of prevention and treatment of NEC in clinic.Erythropoietin(EPO),a glycoprotein hormone,can mediate antioxidative,antiapoptotic,and vessal proliferation.It may play a role in prevention and treatment of NEC.This review will introduce the treatment of EPO and pathogenesis of NEC.

Objective To contrast clinical effect of normal frequency mechanical ventilation vs .nasal continuous positive air‐way pressure ventilation in the treatment of neonatal respiratory failure .Methods Sixty children diagnosed as neonatal respiratory failure were enrolled in our hospital from January 2013 to October 2014 ,and the children were randomly divided into observation group (n=31) and control group (n=29) .Observation group were given nasal continuous positive airway pressure ,children with the control group received normal frequency mechanical ventilation .The X‐ray of the two groups before treatment and 2 d after treatment were observed and scored ,the 3 d survival rate ,blood gas analysis index ,ventilator associated pneumonia ,time of opera‐tion ,and incidence of pulmonary hemorrhage were observed in the two groups .Results The X‐ray results showed that :after treat‐ment ,the two groups of children with X‐ray film scores were gradually decreased ,compared with before treatment ,the difference was statistically significant (P<0 .05);the observation group decreased significantly after treatment 24 h compared with the control group ,the difference was statistically significant (P<0 .05);the survival rate of 3 d in the observation group(93 .50% ) was signifi‐cantly higher than that in the control group(82 .76% ) ,the difference was statistically significant (P<0 .05) .The pH in observation group was less than the control group ,PO2 was greater than the control group ,the difference was statistically significant (P<0 . 05) ,the PCO2 in observation group was less than the control group ,but the difference was not statistically significant (P>0 .05) . Time ,incidence of pulmonary hemorrhage and incidence of ventilator associated pneumonia in observation group were less than those in control group ,the difference was not statistically significant (P>0 .05) .Conclusion The normal frequency of mechanical ventilation and nasal continuous positive airway pressure can be effective reducing the relevance of respiratory pneumonia in the treatment of neonatal respiratory failure ,short‐term effect of nasal continuous positive airway pressure is slightly better than normal frequency mechanical ventilation .

Objective To investigate the influence of serial subcultivation on the immunoregulation of retinal pigment epithelialium cells in vitro.Methods Co-culture-systems of HRPE cells with lymphocytes were established in vitro.The expression of Fas on different passages of HRPE cells and the induction of apoptosis on lymphocytes were observed by flow cytometry. Results When co-cultured with lymphocytes,the expression of Fas was tapering and the duration of culture in vitro was significantly correlated with the expression of Fas(the rate of positive cell: r=-0.859,P0.05).Conclusion HRPE cells beyond 3 passages showed a 1ow capability of immunoregulation and were not suitable for seeds cells for HRPE transplantation.

Objective To investigate the expression of epidermal growth factor-like domain 7 (EGFL-7) in brain tis?sue of diabetes mellitus (DM) rats, and the intervene effect of amioguanidine and baicalein thereof. Methods Forty-eight SD rats were randomly divided into following four groups:control group, diabetic group, aminoguanidine group and baicalein group (n=12 for each group). The control group received only pelleted food and water. Diabetic group, aminoguanidine group and baicalein group were given high fat and sugar diet in combination with small dose of intraperitoneal injection of strepto?zotocin to establish the diabetic model. And then aminoguanidine group and baicalein group were given aminoguanidine and baicalein 150 mg/(kg · d) through oral gavaging. Diabetic group and control group were given equivalent saline through oral gavaging. After 16-week treatment, the expression of EGFL-7 proteins in rat brain was detected by immunohistochemistry and Western blotting methods. Results Results of immunohistochemistry and Western blotting assay showed that the ex?pression of EGFL-7 was significantly increased in diabetic group, aminoguanidine group and baicalein group compared with that of control group (P<0.01). When compared with diabetic group, the expression of EGFL-7 was significantly decreased in aminoguanidine group and baicalein group (P<0.01). There was no significant difference in the EGFL-7 expression be?tween aminoguanidine group and baicalein group (P>0.05). Conclusion EGFL-7 may play an important role in cerebral vascular hyperplasia and remodeling of diabetic rats. Aminoguanidine and baicalein restrained cerebral vessel growth by re?ducing the expression of EGFL-7.

Objective To investigate the expression and significance of Maspin and IKKα in nasosinusoidal mucosa of rats with fungal rhinosinusitis (FRS).Methods A total of 40 SD rats were used to establish the FRS model, and randomly divided into nasal obstruction group, FRS group, immunosuppressive group and invasive FRS group, 10 rats in each group.Another 10 normal rats were used as control group.Mice in the control group were fed with normal diet.In the nasal obstruction group, the mice had only hemostatic cotton stuffed in the nasal cavity and injection of 0.9% NaCl in the abdominal and nasal cavities.In the FRS group, the mice were injected Aspergillus fumigatus spore suspension into the nasal cavity and 0.9% NaCl i.p.The mice of the immunosuppressive group were given cyclophosphamide i.p.and 0.9% NaCl injection into the nasal cavity.The invasive FRS group was injected with cyclophosphamide i.p.and Aspergillus fumigatus spore suspension into the nasal cavity.The serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay (ELISA).The expression of Maspin and IKKα in nasosinusoidal mucosa was detected by immunohistochemical staining.The expression of Maspin mRNA and IKKα mRNA in the nasosinusoidal mucosa was detected by fluorescence quantitative PCR.Results The serum levels of IL-6 and TNF-α in different groups were significantly different (P< 0.05).The level of IL-6 in the FRS group was (69.3 ± 10.9) ng/L, significantly higher than those in the control group, nasal obstruction group, immunosuppressive group and invasive FRS group [(45.2 ± 7.1)ng/L, (46.4 ± 6.7) ng/L, (21.3 ± 4.5) ng/L, and (20.9 ± 4.3 ng/L)] (P < 0.05).The level of TNF-α in the FRS group was (30.4 ± 4.8) ng/L, significantly higher than those in the control group, nasal obstruction group, immunosuppressive group and invasive FRS group [(14.8 ± 2.7) ng/L, (13.9 ± 1.4) ng/L, (7.9 ± 0.6) ng/L, and (7.8 ± 0.4 ng/L)] (P < 0.05).The levels of IL-6 and TNF-α in the control group were significantly higher than those in the immunosuppressive group and invasive FRS group (P< 0.05).There was no significant difference between the immunosuppressive group and the invasive group (P> 0.05).Theresult of immunohistochemical staining showed that the protein expression of Maspin in the FRS group and invasive FRS group was significantly lower than that in the control group, nasal obstruction group and immunosuppressive group, while the expression of IKKα protein was significantly higher than that of control group, nasal obstruction group and immunosuppressive group (P< 0.05).The protein expression of Maspin in the invasive FRS group was significantly lower than that in the FRS group, by contrast, the expression of IKKα protein was significantly higher (P< 0.05).The PCRresult revealed that the expression levels of Maspin and IKKα mRNA were (0.217 ± 0.013) and (0.193 ± 0.012), significantly lower than that in the control, obstruction and immunosuppressive groups [(0.309 ± 0.021), (0.302 ± 0.017), and (0.293 ± 0.02)] (P< 0.05), while the expressions level of IKKα mRNA were significantly higher [(0.319 ± 0.043), (0.384 ± 0.048) vs (0.169 ± 0.015), (0.171 ± 0.018), and (0.175 ± 0.019)] (P< 0.05).Conclusions Down-regulation of Maspin expression after IKKα activation is the main cause of the onset of FRS, which may also be one of the mechanisms of invasive FRS.

Because of the unique molecular structure and stable performance of yolk immunoglobulin ( IgY) , it has been a hot spot in the field of antibody research.As a new type of vaccine, nucleic acid vaccine has more advantages than most traditional vaccines, such as production cost, immunizing dose and immune protection.The approach combining the advantages of nucleic acid vaccine and IgY production in egg yolk and suck IgY has wide scope of application.In this re-view, we introduce a quick and inexpensive production method of IgY, namely, IgY produced by nucleic acid vaccine im-munization.

Objective To compare the clinical effect of UK and rt-PA intravenous thrombolytic therapy for acute myocardial infarction.Methods By SAS statistical analysis system,72 patients with acute myocardial infarction were randomly divided into rt-PA group and UK group,each group had 36 cases.The coronary artery recanalization rate,bleeding events,adverse cardiovascular events,mortality,and cardiac troponin T(cTnT) level were recorded.Results In rt-PA group,0.5h,1 h,2h after the start of thrombolytic therapy,the coronary artery recanalization rate was significantly higher than that in the control group,the difference was statistically significant (x2 =7.32,6.12,5.81,all P ＜ 0.05).In rt-PA group,3 cases had bleeding events,2 cases died,and in UK group,5 cases had bleeding events,3 cases died,,there was no statistically significant difference between the two groups (x2 =1.22,P ＞ 0.05).In rt-PA group,there were 6 cases with adverse cardiovascular events,14 cases in the control group,the difference was statistically significant (x2 =4.56,P＜0.05).2 cases died in rt-PA group,3 cases died in UK group,the mortality between the two groups had no statistically significant difference (P ＞ 0.05).The cTnT level in the hospital 6h,12h was significantly higher than admission in two groups (t =6.67,6.30,4.11,4.22,4.40,all P ＜ 0.05).In rt-PA group,cTnT level in the hospital 6h,12h was significantly lower than that in UK group (t =5.92,6.61,all P ＜0.05).Conclusion Compared with UK,rt-PA in the treatment of acute myocardial infarction can increase recanalization rate,decrease adverse events and myocardial injury,it is worthy of clinical application.

BackgroundWith the number of diabetics increases,people pay more attention to the diabetic keratopathy.The major mechanism leading to diabetic keratopathy is diabetic corneal neuropathy.So it is significant to observe pathologic mechanism of diabetic corneal neuropathy. Objective To investigate the protective effects of edaravone( a free radical scavenger) on corneal nerve of rats with experimental diabetic corneal neuropathy,then explain the effects of oxidative stress in the pathologic mechanism of diabetic corneal neuropathy. Methods Seventy Sprague-Daxley male rats were taken as experimental subjects and 20 of them were used as normal control group.The remaining 50 were induced to be diabetic mellitus by a single intraperitoneal injection of streptozotocin and divided into 2 groups randomly:edaravone treated group and diabetic control group.In the edaravone treated group,edaravone(0.2 g/L) eye drops were used 3 times a day until the animal was killed.Five rats in each group were sacrificed at 6,8,10 and 12 weeks respectively.Then the corneal sensation,number of corneal nerve fibers,morphology,content of malondialdehyde(MDA) and activity of superoxide dismutase(SOD) in the corneal tissue were detected.ResultsIn the diabetic control group,the corneal sensation and the number of corneal nerve fibers were decreased,the density of neural network for cluster was sparse,the nerve activity was decreased,the content of MDA in the corneal tissue was significantly increased,the activity of SOD in the corneal tissue was significantly decreased (P＜0.01 ).Accompany with the course of disease,the above change was obvious day after day.Compared with the diabetic control group,the corneal sensation and the morphological abnormalities in corneal nerve of edaravone group were improved significantly which had the partial branches to the 12th week,the content of MDA in the corneal tissue was significantly decreased,the activity of SOD in the corneal tissue was significantly increased (P＜0.01).Conclusions Edaravone can lower diabetic corneal nerve of rats with experimental diabetic corneal neuropathyinjury,Oxidative stress may be a critical pathologic mechanism of diabetic corneal neuropathy.