The event of “diagnosing mental disorder but actually not” violates patients′right of informed con-sent and refused to health , showing some problems of “diagnosing mental disorder but actually not” and mandatory admission process and psychiatric judicial authentication , and also have some ethical problems .Therefore, relevant departments should perfect the laws and regulation , establish a hospitalized psychiatric patients appeal , the appeal channels , at the same time , physicians should follow a correct understanding , respect patients rights , disinterest-ed, the ethical principles of self -supervision and also use constraints should be regulated .

OBJECTIVE:To evaluate the effect of temozolomide(TMZ)in combination with dexamethasone(DXM)on the proliferation of human gliocytoma U251 cells in vitro.METHODS:Human U251 cells were assigned to 1 of the 3 groups: TMZ(10,25,50,100,200,400?mol?L~(-1),respectively)alone or in combination with 40?mol?L~(-1)DXM(TMZ+DXM group)or control group(none drug).After treatment for 72 hours,the cell morphology,cell inhibition ratio,cell cycle and the apoptotic rate were detected.RESULTS:When TMZ concentration was greater than 100?mol?L~(-1)the cell inhibition ratio was higher in TMZ-treated group than in TMZ+DXM-treated group(P

BackgroundWith the number of diabetics increases,people pay more attention to the diabetic keratopathy.The major mechanism leading to diabetic keratopathy is diabetic corneal neuropathy.So it is significant to observe pathologic mechanism of diabetic corneal neuropathy. Objective To investigate the protective effects of edaravone( a free radical scavenger) on corneal nerve of rats with experimental diabetic corneal neuropathy,then explain the effects of oxidative stress in the pathologic mechanism of diabetic corneal neuropathy. Methods Seventy Sprague-Daxley male rats were taken as experimental subjects and 20 of them were used as normal control group.The remaining 50 were induced to be diabetic mellitus by a single intraperitoneal injection of streptozotocin and divided into 2 groups randomly:edaravone treated group and diabetic control group.In the edaravone treated group,edaravone(0.2 g/L) eye drops were used 3 times a day until the animal was killed.Five rats in each group were sacrificed at 6,8,10 and 12 weeks respectively.Then the corneal sensation,number of corneal nerve fibers,morphology,content of malondialdehyde(MDA) and activity of superoxide dismutase(SOD) in the corneal tissue were detected.ResultsIn the diabetic control group,the corneal sensation and the number of corneal nerve fibers were decreased,the density of neural network for cluster was sparse,the nerve activity was decreased,the content of MDA in the corneal tissue was significantly increased,the activity of SOD in the corneal tissue was significantly decreased (P＜0.01 ).Accompany with the course of disease,the above change was obvious day after day.Compared with the diabetic control group,the corneal sensation and the morphological abnormalities in corneal nerve of edaravone group were improved significantly which had the partial branches to the 12th week,the content of MDA in the corneal tissue was significantly decreased,the activity of SOD in the corneal tissue was significantly increased (P＜0.01).Conclusions Edaravone can lower diabetic corneal nerve of rats with experimental diabetic corneal neuropathyinjury,Oxidative stress may be a critical pathologic mechanism of diabetic corneal neuropathy.

There are one third of synthesized proteins must be secreted to the cell surface or to the surrounding environment to acquire their native functional state. Most of them are exported by Sec translocase (secretion pathway). Sec translocase consists of a membrane embedded protein-conducting channel, termed SecYEG and a peripherally associated motor domain, the ATPase SecA. The SecDFyajC heterotrimeric membrane protein complex can facilitates protein translocation. SecB is a molecular chaperone that functions in the protein translocation pathway. SecM (secretion monitor) encoded by the 5' region of the secM-secA mRNA, which elongation arrest is required for upregulated expression of SecA. The signal sequence in the N terminus of the nascent peptide is first recognized by the signal recognition particle (SRP). SecB, the Sec-system-specific chaperone, channels the preprotein to the Sec translocation pathway and, ad- ditionally, actively targets the bound precursor to the translocase by its ability to bind SecA. The preprotein-bearing SecA then binds to the membrane, at a high-affinity SecA-binding site, SecYEG, which constitutes a channel for polypeptide movement. Continued translocation requires cycles of ATP hydrolysis bySecA, which is thought to occur in a step-wise fashion with a step of 20~30 amino acid residues.

Objective Systematically reviewed the standard treatment of primary brain glioma patients high levels,and discussed effective new therapies. Surgery,radiotherapy and chemotherapy are the main methods of treatment of high?grade gliomas, molecular markers supplement clinical prognostic factors under certain circumstances be able to guide treatment decisions. The rapid development of technology using molecular data can improve our understanding of the disease pathogenesis and proliferation. Effectively validate prognostic biomarkers and identification of tumor may improve the outcome.

Objective To investigate the drug resistance of clinical isolates in Pinggu area from 2011 to 2013,and guide clinical rational use of antibiotics.Methods Isolates from 2011 to 2013 in the hospital were collected and identified to species.Antibiotic susceptibility test were performed.For the infection with the same position and the same patient,only the first isolate was included. WHONET5.6 data analysis software was used for data auditing and inputing,the USA CLSI M100-S22 standard were refered to. Results Total of 5 794 strains of clinically isolated pathogenic bacteria were collected from 2011 to 2013,1 600 strains in 2011, 2 234 strains in 2012,1 960 strains In 2013.The detection rate of MRSA in each year from 2011 to 2013 were 63.50%,65.00%, 65.30% respectively.No vancomycin and linezolid-resistant Staphylococcus aureus was found.The resistance rate of Enterococcus to teicoplanin was <5%,to Linezolid <2.4% and to vancomycin <21.1%.The resistance rates of Escherichia coli and Klebsiella pneumoniae to imipenem and meropenem were both < 1.3%.The annual resistance rates of Acinetobacter bauman to imipenem were 34.6%,26.9%,29.3% respectively.Resistance rates of Pseudomonas aeruginosa to imipenem,meropenem,cefoperazone/shu-batan,piperacillin/tazobactam were lower than the national average.No polymyxin B resistance isolate was found.Stenotroph-omonas maltophilia were sensitive to ceftazidime and minocycline.Conclusion The antibiotic resistance rate data in Pinggu exhibits area specificity,which was different from the national antimicrobial resistance monitoring data in 2011.Detection rate of MRSA,re-sistance rate of enterococci to linezolid and vancomycin,Escherichia coli,Klebsiella pneumoniae,Enterobacter cloacae to imipenem, meropenem are increasing year by year.The resistance rates of Acinetobacter bauman and Pseudomonas aeruginosa are lower than the national average rate.

BACKGROUND: Studies on encapsulated whole cel protein antigen ofHelicobacter pylori are stil at the exploration stage. There is limited literature concerning the preparation process andin vitro release characteristics of chitosan microspheres encapsulated with whole cel protein antigen ofHelicobacter pylori. OBJECTIVE:To explore the preparation process andin vitrorelease characteristics of chitosan microspheres encapsulating whole cel protein antigen ofHelicobacter pylori. METHODS: Precipitation method was used to prepare chitosan microspheres, and the best preparation process, matching and encapsulation time were screened. Under electron microscope, the morphology and particle size of microspheres were observed. Chitosan microspheres were used to encapsulateHelicobacter pylori whole cel protein antigen, and BCA method was used to determine encapsulation efficiency, encapsulation content and release efficiency in vitro of Helicobacter pylori whole cel protein antigen. RESULTS AND CONCLUSION:Final concentration of 1% glacial acetic acid, sodium sulfate as crosslinking agent, pH=5.0, with no pulverization when the crosslinking agent was added was the best preparation process for chitosan microspheres. Electron microscopy showed the smooth surface morphology of microspheres with roundness and good dispersion, and the majority of the microspheres were 1-5 μm in diameter. The encapsulation efficiency ofHelicobacter pylori whole cel protein antigen microspheres was 80.4%, the encapsulated amount was 16.4%, and total 48-hour release rate was 19.4%.Helicobacter pylori whole cel protein antigen microspheres showed an overal slow release status. Chitosan microspheres show good encapsulation efficiency and amount ofHelicobacter pylori whole cel protein antigen, and Helicobacter pylori total bacteria protein antigen microspheres show an overal slow release status.

Objective To construct a new gene delivery system based on atelocollagen (ATE),and explore that modified aptamer (APT),and APT-ATE/miRNA (miRNA-15a and miRNA-16-1) were successfully synthesized to treat bone-metastatic prostatic cancers.Methods Flow cytometry (FCM) analysis was used to characterize APT-ATE complex.The diameter and zeta potential of complexes were measured by Zetasizer Nano-ZS9.The prostatic cancer (PCa) distribution experiments were used to explore its biological characteristics and targeting ability of PCa cells (PC3 and LNCaP).The inhibition of APT-ATE complex on LNCaP cell was determined with the cholecystokinin (CCK)-8 assay.Results FCM results demonstrated the successful synthesis of ATE-APT complex.The cellular uptake of vectors was concentration-dependent.The gene expression in vitro indicated that the modification of APT could increase the efficiency of gene expression and PCa targeting ability of ATE vectors to LNCaP [prostate specific membrane antigen (PSMA) over-expressing prostate cancer cells].The result of biodistribution showed that the bone uptake of APT-ATE was higher than ATE-APT.Conclusions APT-ATE/miRNA might be useful for preclinical and clinical studies on the treatment of bone-metastatic PCa.

Objective To study the clinical effects of femtosecond laser surgery and phacoemulsification in patients with cataract. Methods A total of 80 patients were randomly divided into study group and control group. 40 patients with 44 cataract eyes in control group were treated by routine phacoemulsification and 40 patients with 47 cataract eyes in control group were treated by femtosecond laser additionally. The capsulotomy diameter, capsular tension ring, capsule size and operation time difference were compared between two groups. The vision and Intraocular lens decentration rate were compared between two groups. Results The capsulotomy diameter in study group was significantly less than control group (P < 0.01). The apsular tension ring in study group was significantly more than control group(P < 0.05). The capsule size in study group was significantly less than control group (P < 0.01). The operation time in study group was significantly less than control group (P >0.05). The vision in study group was significantly better than those in control group during a year (P < 0.05) and the adverse reaction rate was significantly less (P < 0.05). Conclusion Femtosecond laser combined with phacoemulsification has a great clinical effects which improve patients′ vision and reduce adverse reaction rate makes it worth for clinical application.