Objective To explore the feasibility, difficulties and indications of video-assisted laparoscopic pancreaticoduodenectomy. Methods Clinical records of 5 cases of laparoscopic pancreaticoduodenectomy in this hospital from November 22, 2002 to April 28, 2005 were analyzed, with respect to preoperative diagnosis, surgical methods and procedures, intraoperative blood loss, operation time, difficulties encountered and countermeasures, and postoperative recovery. Related parameters included patient's temperature, drainage volume, blood routine, liver functions, amylopsin changes, postoperative complications, bowel movement recovery, and length of hospital stay. Results All the 5 cases of operation were successfully performed, with an operation time of 360~660 min (mean, 528 min) and an intraoperative blood loss of 150~2 000 ml (mean, 770 ml). Pathological examinations revealed 3 cases of well-differentiated papillary adenocarcinoma of the duodenum, 1 case of endocrine small-cell carcinoma of the head of pancreas, and 1 case of papillary adenomatoid hyperplasia of the duodenum with malignant changes accompanying intermediated-differentiated adenocarcinoma of upper and middle intrahepatic biliary duct. Postoperatively, a small amount of pancreatic leakage occurred in case number 1 and a digestive tract stress ulcer happened in case number 2, all of which were cured by symptomatic management. In case number 4, a recurrent pancreatitis with pulmonary infection was observed, and the patient died from stress ulcer hemorrhage on the 39th postoperative day. Except for the case number 4 in which the patient's highest temperature reached 39.5 ℃ after the recurrence of pancreatitis, the highest temperatures of the other 4 patients were all

Biomedical Research ; Humans ; Tai Ji

To discuss the feasibility of the pharmacodynamic evaluation method for traditional Chinese medicine (TCM) formula particles, with traditional decoction for reference and the intervention of Magnoliae Officinalis Cortex in rats with ulcerative colitis (UC). First of all, the similarity of traditional Magnoliae Officinalis Cortex decoction and formula particles of different manufacturers was defined by using the IR fingerprint. The UC rat model was established and given Houpo formula particles of different doses and manufacturers, with the decoction for reference, in order to observe disease activity index (DAI), colon mucosa damage index (CMDI), pathologic changes, nitric oxide (NO), endothdin (ET), substance P, vasoactive intestinal peptide (VIP). Their intervention effects on UC rats were compared to study the difference between Sanjiu and Tianjiang Houpo formula particles, in order to demonstrate the feasibility of the pharmacodynamic evaluation method for Houpo formula particles. According to the results, Houpo formula particles showed similar pharmacodynamic actions with the traditional decoction. The pharmacodynamic comparison of Houpo formula particles of different manufacturers showed no statistical significance. The experiment showed that on the basis of the TCM compounds, a prescription dismantlement study was conducted to define target points of various drugs. The traditional decoction was selected for reference in the comparison of corresponding formula particles for their pharmacodynamic equivalence. This method could avoid controversies about single or combined boiling of formula particles, and give objective comments on the pharmacodynamic effect of the formula particles. The method is proved to be feasible.
Animals ; Chemistry, Pharmaceutical ; methods ; Colitis, Ulcerative ; drug therapy ; metabolism ; Dosage Forms ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal ; adverse effects ; chemistry ; pharmacokinetics ; Humans ; Magnolia ; chemistry ; Male ; Rats ; Rats, Wistar ; Substance P ; metabolism ; Vasoactive Intestinal Peptide ; metabolism

Biomarkers, Tumor ; analysis ; Humans ; Male ; Prostate-Specific Antigen ; analysis ; Prostatic Neoplasms ; metabolism

Objective In order to discuss the nursing points for chronic skin ulcer patients who had ac-cecpted local oxygen therapy and traditional chinese medicine treatment. Methods We gave totally nursing to 20 patients who accecpted local oxygen therapy and traditional chinese medicine treatment. Results Eighty percent patients had obtained clincal cure and got successful nursing effect. Conclusions Careful perioperation nursing and related monitoring can improve the cute rate of chronic skin ulcer, and then pre-vent complication.

Objective To assess the therapeutic effect of cerebral aneurysmal embolization with electro-detachable platinum coils(EDPC), and to discuss the timing of the embolization and points for attention during the procedure. Methods From February 1999 to July 2004, 60 intracranial aneurysms were treated with EDPC in 58 patients, of which 56 cases were subject to subarachnoid hemorrhage (SAH). 36 patients were Hunt & Hess Grade I, 12 Grade II and 8 Grade IV～V. Two of all 58 patients had no SAH. Two sorts of EDPC were used in this group: one is Guglielmi detachable coil from Boston Scientific Corporation and the other is Sapphire detachable coil from Micro Therapeutics Inc. All the sessions of embolization and each detachment of the EDPC were closely monitored both fluoroscopically and angiographically for the accurate and safe embolization of aneurysmal lumen. Results Of all 60 aneurysms, 58 were successfully treated with EDPC and 2 were failed to be embolized due to arterial spasm. The packing degree of aneurismal lumen was complete in 44 cases (75.9%) and incomplete in 12 cases (20.7%). The incidence of non-thrombotic complication was 6.9%. One patient had hemiplegia because of the hernia into the parent artery by EDPC. No death occurred in our group. 41 patients were followed-up during a period of 1～4 years and no SAH onset recurred after embolization. Conclusions Endovascular treatment of intracranial aneurysms with GDC is a better alternative. But the long-term effects should be verified by follow-up in the future.

The hybrid PET/MR has a unique advantage of simultaneous scanning of both PET and MRI images,which has been gradually applied in clinical practice.In the clinical studies of severe brain diseases (such as cerebrovascular disease,brain tumor and epilepsy),accurate quantification of cerebral blood flow (CBF) can help to understand the etiology,pathogenesis,and to make early diagnosis as well as therapeutic solutions.The hybrid PET/MR can implement a noninvasive,convenient and accurate method of arterial input function for quantification of CBF.The application of the hybrid PET/MR in quantification of cerebral blood flow were reviewed in this article.

Objective To probe the application of CT cerebral perfusion imaging on transient ischemic attacks(TIA) Methods Conventional CT and CT cerebral perfusion imaging were performed on 5 normal adults and 20 patients with clinically diagnosed TIA After regular CT examination, dynamic scans of 40 seconds were performed on selected slice (usually on the basal ganglia slice), while 40 ml non ionic contrast material were bolus injected through antecubital vein with These dynamic images were processed with the "Perfusion CT" software package on a PC based workstation Cerebral blood flow (CBF) and time to peak (TP) enhancement were measured within specific regions of the brain on CT perfusion images Quantitative analysis was performed for these images Results A gradient of perfusion between gray matter and white matter was showed on CT perfusion images in normal adults and TIA patients CBF and TP for normal cortical and white matter were 378 2 ml?min -1 ?L -1 , 7 8 s and 112 5 ml?min -1 ?L -1 , 9 9 s, respectively In 20 cases with TIA, persisting abnormal perfusion changes corresponding to clinical symptoms were found in 15 cases with prolonged TP Other 5 cases showed normal results TP of affected side (11 8?4 4) s compared with that of the contralateral side (9 1?3 1) s was significantly prolonged ( t =5 277, P

Objective To determine the changes in action potential peak value and maximal nerve conduction velocity of rabbit sciatic nerve after being exposed to different doses of high intensity ultrasound (HIU) and establish the experimental basis for the use of HIU in patients with refractory pain. Methods Eighty New Zealand white rabbits of either sex weighing 2.0-2.5 kg, aged 5-10 months were randomly divided into four groups with 20 rabbits in each group :group Ⅰ 0 second;group Ⅱ 15 seconds;group Ⅲ 25 seconds and group Ⅳ 50 seconds. The animals were anesthetized and placed in the prone position. Sciatic nerves were exposed to 4 different doses of HIU (7.1 MHz, 40 W/cm2 f55 0 s, 15 s, 25 s, 50 s) in the four groups. The animals were allowed to recover. At 5 intervals (1 and 2 weeks, 1, 3 and 6 months) after exposure to HIU, 4 animals in each group were anesthetized and recording electrodes were placed on sciatic nerves at 2 cm distal to the site of HIU radiation, tibial and sural cutaneous nerves. The amplitude (peak value) and latency of action potential and maximal conduction velocity of the nerves were recorded. Sciatic nerves were obtained from one of the four animals for light and electron microscopic examination. Results The larger was the dose of HIU, the more time was needed for the injuried nerve to recover. In group Ⅰ (0 s) there were no significant changes in action potential peak value and maximal nerve conduction velocity at any interval after exposure to HIU;in group Ⅱ (15 s) there were slight changes in the electrophysiological parameters and the changes recorded on tibial nerve returned to normal soon while those recorded on sural cutaneous nerve returned to normal one month later; in group Ⅲ (25 s) there were significant changes in the electrophysiological parameters and it took 3 months for tibial nerve but 6 months for sural cutaneous nerve to recover;in group Ⅳ (50s) the nerves were completely blocked, no action potential was recorded and no recovery was observed after 6 months. Light and electron microscopic examination showed that in group 1 no significant changes of nerves were observed; in group Ⅱ there was slight deformation of myelin sheath which returned to normal 2 weeks later; in group Ⅲ sheath was broken or lost, and axon degeneration was observed at 1 week after exposure to HIU and the changes disappeared 1 month later; in group Ⅳ necrosis of the nerve was observed and no recovery was observed 6 months later. Conclusion HIU can be used for the treatment of refractory pain. Analgesia can be achieved with loss of only part of motor function. The nerve can be permanently blocked by HIU if necessary.