Eighty-seven patients with hyperthyroidism were enrolled in a retrospective study,and 35.6％ of them also suffered from pulmonary hypertension(PH).Advanced age and systolic or diastolic dysfunction of left heart were risk factors in the mechanism of PH associated with hyperthyroidism (all P＜0.05).However,autoimmune disorder was found as a non-related factor in the mechanism of PH(P＞0.05).

Adult ; Decompression Sickness ; Diving ; Humans ; Male ; Middle Aged ; Occupational Diseases

Objective To assess the value of ultrasoud examination with transvaginal scaning(TVS) in the diagnosis of tubal pregnancy(TP). Methods 40 cases of clinically suspected TP were exeamined by two ultrasoud approaches: transvaginal and transabdominal prior to surgery.Based on surgery and pathology ,the sonographic manefestutions and diagonostic results of both methods were compared and analized statisticlly. Results The TP masses were visualized in 36 cases in TVS group(36/40) as compared with 27 cases in TAS group (27/40) (? 2=7.364, P

Adult ; Hearing Loss, Noise-Induced ; etiology ; Hearing Tests ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Young Adult

Objective: To observe the clinical efficacy of Tuina (Chinese therapeutic massage) plus Shen Ling Bai Zhu San for pediatric diarrhea due to spleen deficiency.Methods: A total of 105 children with diarrhea due to spleen deficiency were selected and randomized into two groups by the random number table method. Fifty-two cases in the control group were treated with oral Shen Ling Bai Zhu San, and 53 cases in the observation group were treated with additional Tuina treatment. The clinical efficacy was evaluated after treatment, and the changes of traditional Chinese medicine (TCM) symptom scores were observed. Results: During the trial, there were three dropouts in the observation group, and two in the control group. After the treatment, the total effective rate was 100.0％ in the observation group, and 90.0％ in the control group. The difference between the two groups was statistically significant (P<0.05). The scores of primary and secondary symptoms, and the total scores of TCM symptoms in both groups were significantly lower than those before treatment (P<0.05). And the scores of primary and secondary symptoms, and the total score of TCM symptoms in the observation group after treatment were lower than those in the control group (P<0.05). There were no obvious adverse reactions in either group during the treatment. Conclusion: Tuina plus Shen Ling Bai Zhu San has a better therapeutic effect in treating pediatric diarrhea due to spleen deficiency than Shen Ling Bai Zhu San alone.

The serum specimens were collected from ovarian cancer (n =100),benign ovarian disease (n =69) and healthy women (n =95).The serum levels of HE4 and CA125 were detected.Risk of ovarian maliqnancy algorithm (ROMA) was calculated.Accuracy of prediction was evaluated by the area under receiver operating characteristic curve (ROC-AUC).And validity of prediction was evaluated by sensitivity and specificity.The results showed that the median level of ROMA algorithm was 83.0％,8.9％ and 8.7％ in ovarian cancer,benign ovarian disease and healthy women groups respectively.The difference were statistically significant (all P ＜ 0.01).Compared with benign ovarian disease group,the ROC-AUC of ROMA algorithm was 0.900 in ovarian cancer group.The sensitivity and specificity were 81.0％ and 92.8％ in ovarian cancer group respectively.Thus ROMA algorithm is a useful parameter in risk stratification for ovarian cancer.The diagnostic accuracy of ROMA algorithm is better than that of HE4 and CA125 in ovarian cancer.

AlM: To observe the treatment efficacy of eyelid herpeszoster with external use ganciclovir ophthalmic gel.METHODS:A total of 77 patients were randomly divided into treatment group and control group. ln the treatment group, ganciclovir ophthalmic gel was topical coated on the basis of antiviral drugs, while the control group was coated with conventional acyclovir ointment and antiviral treatment.
RESULTS: The cure rate was 95% in the treatment group, and the control group was 83% after 10d treatment. The difference was significant.
CONCLUSlON: The efficacy of ganciclovir ophthalmic gel treated eyelid herpes zoster is obvious.

This paper summarizes the features of web sites that would be useful to infectious diseases physicians by exploring the Internet through search engine including Google,Baidu and Yahoo.Meanwhile,suggestions from professional forums,web sites and publications are also taken into consideration.Nine Comprehensive sites containing three categories and more of microbial pathogens,nine special sites for parasites,four special sites for fungi,two special sites for viruses and two special sites for bacteria are collected.Subjective navigation for each site is given.Features of these sites,including laboratory images,clinical images and number of images are also described.

BACKGROUND:The hair fol icle stem cells are usual cultured in Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum. Recently, keratinocyte serum-free medium has been used to culture hair fol icle stem cells.
OBJECTIVE:To observe the effects of three different culture media on the proliferation and purity of hair fol icle stem cells.
METHODS:After sterling, the skin of the rat’s beard was cut off, and the vibrissa fol icles were dissected under a stereocroscope. The fol icles were digested by Dispase Ⅱ firstly and by the mixture of trypsin and EDTA secondly. cellsuspension was divided into three pieces based on the average number of cells and respectively cultured by keratinocyte serum-free medium, Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum and keratinocyte serum-free medium containing 10%fetal bovine serum. Hair fol icle stem cells were selected by rapid adherence on col agen type Ⅳ, and passaged.
RESULTS AND CONCLUSION:Cultured hair fol icle stem cells spread to the third generation, and trypan blue assay showed there was no difference in cellsurvival rate among the three groups (P>0.05). cellCounting Kit 8 assay showed that the cells grew slowly in the three groups in the first 2 days. At day 4, the hair fol icle stem cells grew into the growth logarithmic phase, and the proliferative activity was ranked as fol ows:keratinocyte serum-free medium+10%fetal bovine serum>Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum>keratinocyte serum-free medium with a significant difference between the three groups (P<0.05). Flow cytometric examination showed the expression of CD34 in the keratinocyte serum-free medium+10%fetal bovine serum group was lower than that in the keratinocyte serum free medium group (P<0.05). The expressions of CD34, CK15 andβ1-integrin (CD29) in the Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum were lower than those in the other two groups (P<0.05). These findings indicate that we can obtain higher purity hair fol icle stem cells in the keratinocyte serum-free medium+10%fetal bovine serum than in the Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum.

Objective Hair follicle stem cells (HFSCs) can be cultured in vti ro to differentiate into chondrocytes, osteoblasts and adipocytes .The aim of the article was to investigate the culture method of HFSCs and their adipogenic and osteogenic differentiation potentials. Methods Microdissection and two-step enzyme digestion were applied to obtain HFSCs , and HFSCs are purified by differ-ential adhesion method.The cells were cultured in keratinocyte serum-free medium obtaining 10% fetal bovine serum.Flow cytometry was used to detect CD34、β1-integrin and CK15.The third-generation HFSCs were cultured by osteogenic inductor and adipogenic induc-tor respectively . Results Cultured HFSCs were homogeneous in shape , bearing the property of great refraction and typical cobble-stone-morph.By flow cytometry, the expression rates of CD34,β1-integrin and CK15 were 59.6%, 97.0%and 61.1%.Stem cells were in good growing condition from the third-generation cell growth curve.21 days after being induced by osteogenic inductor, calcified nodules appeared after alizarin red staining, while 14 days after being induced by adipogenic inductor, intracellular lipid droplets could be observed after oil red O staining. Conclusion Cultured HFSCs are chaterized by strong growth capacity and multiple differentiation potential .