Objective This study was performed to find the association of the severity of coronary artery disease with ordinary risk factors for CHD and left ventricle heart function in patients with CHD. Methods Selective coronary angiography were carried on 305 consecutive patients admitted to our hospital from December 2008 to April 2009. The severity of coronary disease was accessed by the Gensini scoring system. These patients were divided into two groups, patients with CHD group (251 cases) and control group (patients without CHD) (54 cases) , to compare the difference of ordinary risk factors for CHD between the two groups. Multiple linear regression analysis was used to analyze the association of the severity of coronary artery disease with ordinary risk factors for CHD. We also used linear correlation analysis to investigate the association of the severity of coronary artery disease with left ventricle ejection fraction in patients with CHD. Results The two groups had statistically difference on age, sex, smoking index, hypertension prevalence rate, serum glucose abnormal prevalence rate, diabetes prevalence rate, and metabolic syndrome prevalence rate( P < 0.05). We found that age (β_1 =0.251, P <0.01), sex (β_2 =0.235, P < 0.01), LDL-C concentration (β_3 = 0.241,P <0.01), duration of diabetes (β_4 =0.226, P <0.01) went into multiple linear regression model. We also found the severity of coronary artery disease had a negative correlation ( r = -0. 362, P <0.01) with left ventricle ejection fraction in patients with CHD. Conclusion When we decide to cany out coronary angiography on susceptible patients, we should consider their age, sex, smoking index, whether had hypertension, serum glucose abnormal, diabetes, metabolic syndrome or not. Age, sex, LDLC concentration, duration of diabetes affect severity of coronary artery stenosis independently. Left ventricle ejection function will decrease as coronary atherosclerosis continues.

?Retinoblastoma is a common eye malignant tumor which has high degree of malignancy.The traditional treatment methods are destructive, and the prognosis is poor. Hypoxia-inducible factor-1αhighly express in retinoblastoma and regulate the development and progression of retinoblastoma ( by influencing the proliferation and differentiation of tumor cells and involving in the generation of vascular mimicry ) . This article reviewed the role of hypoxia inducible factor-1αin the development of retinoblastoma which will initiate a new avenue for the treatment of retinoblastoma.

Objective To invesgate hepatitis B c antigen(HBeAg)as the carrier to construct mixed hepatitis C virus(HCV)therapeutic vaccine.Methods Fused the pTrc-core gene with two synthetic T-epitope antigen gene of HCV,expressed the plasmids pTrc-core-T1 and pTrc-core-T2, applied sucrose density gradient centrifugation to get the fusion protein HBcAg-T1 and HBcAg-T2, dialysised and concentrated the protein,mixed and immunized them in mice using the protein HBcAg (expressed by pTrc-core)as control.The tumor regression trial in mice was evaluated at appropriate time.After immunized four times,got the blood and spleen of mice.Interleukin(IL)-12 in serum were detected by enzyme-linked immunosorbent assay(ELISA),nonspecific T lymphocytes prolifera- tion response of splenic lymphocytes was respectively examined by thiazolyl blue(MTT)assay.T cell subset of splenic lymphoeytes,IL-5 in serum,IL-4 and interferon(IFN)-?in lymphocyte were evalu- ated by FACS.Results Tumor regression trial showed the experimental group formed only one tumor(diameter=0.1 cm),smaller than the T_1T_2 peptides group(diameter=0.9 cm)and blank group(diameter=1.3 cm).FACS indicated that CD8~+ T cell percentage of spleen cells from HBcAg- T_1T_2 group(20.21?2.01)% was higher than T_1T_2 peptides group(15.33?1.45)% and blank group(5.09?1.66)%,the percentage of IFN-?positive cells in these three groups were(1.58?0.05)%,(0.88?0.02)% and(0.53?0.03)%.The ELISA discovered that the level of IL-12 in the experimental group was the highest.Different from above,the IL-4 and IL-5 were lower in the exper- imental group.The detection of eytotoxic T lymphocyte(CTL)activity showed that the quantity of Hela cells infected by HCV in HBcAg-T1T2 stimulated group was different obviously from T1T2 peptides group. Conclusion The mixed protein HBcAg-T1 and HBcAg-T2 can induce stronger cellular immunity and it is able to serve as a therapeutic vaccines candidate specific for HCV.

Animals ; Cadmium ; toxicity ; Cytochrome P-450 Enzyme System ; blood ; Female ; Ovary ; drug effects ; metabolism ; Phosphoproteins ; blood ; Progesterone ; biosynthesis ; Rats ; Rats, Wistar

BACKGROUND:The rise of tissue engineering has opened up new ways for tissue repair and reconstruction of the urinary tract, and the bladder acellular matrix is a better alternative material for urinary tissue engineering.
OBJECTIVE:To construct the compound of hair fol icle stem cells with heterologous bladder acellular scaffold, and to observe the growth of hair fol icle stem cells on the scaffold.
METHODS:Bladder acellular matrix from New Zealand rabbits were prepared and detected using scanning electron microscopy and Masson staining. Passage 3 hair fol icle stem cells were statical y inoculated into the surface of bladder acellular matrix using secondary sedimentation method. Under inverted microscope, cellgrowth was observed, and cellgrowth curves were drawn. cellgrowth on the scaffold surface was observed through histological detection and scanning electron microscope observation.
RESULTS AND CONCLUSION:Prepared bladder acellular matrix was a white translucent film with fiber mesh structure, and no residual cells were seen. Masson staining results indicated that the bladder acellular matrix had col agen structure, and no obvious residual cells. After culture for 48 hours, hair fol icle stem cells grew wel around the bladder acellular matrix under inverted microscope;1 week later, hair fol icle stem cells extended and adhered to the scaffold surface. These findings indicate that hair fol icle stem cells have a good biocompatibility with the bladder acellular matrix through in vitro culture.

Objective To observe the structural abnormalities of the painful shoulder in patients soon after stroke by M RI,and to identify the possible causes of post-stroke shoulder pain (PSSP).Methods Patients with hemiplegia following their first cerebrovascular accident were divided into 2 groups:PSSP group (32 patients) and post-stroke no shoulder pain (PSNSP) group (20 patients).Physical findings of affected shoulder and MRI findings of both affected and unaffected shoulders were evaluated.Results Shoulder subluxation was significantly more frequent in PSSP group than in PSNSP group (53％ vs 15％,P ＜0.05).In PSSP group,the incidence rate of rotator cuff injury,subacromial-subdeltoid bursa effusion,glenohumeral joint effusion,effusion of long head of biceps tendon was higher in affected side than that in unaffected side(63％ vs 13％,53％ vs 13％,75％ vs 31％,41％ vs 13％,respectively).In PSNSP group,the incidence rate of glenohumeral joint effusion was higher in affected side than that in unaffected side (70％ vs 17％).But compared with no pain hemiplegic shoulder,only rotator cuff injury and subacromial-subdeltoid bursa effusion were significantly higher in PSSP patients (63％ vs15％,53％ vs 25％,respectively),and there was significant difference between the two groups (P ＜0.05).Conclusion Rotator cuff injury was found to be a possible cause of PSSP.

Radiotherapy is an effective mean of treatment of gastric cancer. Combination of radiotherapy and chemotherapy in gastric cancer preoperative, postoperative and palliatie treatments can increase the local control rate and the survival rate of gastric cancer patients. With the development of three-dimensional conformal and intensity modulated radiotherapy, the radiotherapy is being more extensively applied in the comprehensive treatment of gastric cancer.

Objective To observe the inhibitory effects and characteristics of intravitreal injection with bevacizumab on laser-induced choroidal neovascularization (CNV).Methods Twelve male brown norway (BN) rats were divided into the bevacizumab group and control group with six rats in each group.One eye of rats were received a series of 8 diode laser esions around optic disc to induce CNV,then the rats in bevacizumab group and control group underwent intravitreal injection with 2 μl bevacizumab and ringer's lactate.On days 7,14,and 21,the morphology and leakage of CNV were observed by fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA).On day 21 after photocoagulation,thephotocoagulated eyes were enucleated and processed for histopathologie examination,including hematoxylin and eosin (H&E) staining and immunohistochemistry staining for vascular endothelial growth factor (VEGF).Results On day 7 after photocoagulation,ICGA showed that CNV developed in the bevacizumab group and the control group.FFA showed that leakage intensity in the bevacizumab group was significantly lower than that in the control group,but the bevacizumab group gradually increased over time.The mean thickness of CNV significantly decreased in the bevacizumab group.The CNV in the bevacizumab group were negative for VEGF according to the result of immmuohistochemistry staining.Conclusions Early intravitreal injection with 2 μl bevacizumab can reduce the thickness of CNV and inhibit the leakage of CNV.However,bevacizumab could neither block the formation of CNV,nor suppress the permeability permanently.Combined other therapies with bevacizumab may be more potential to treat CNV effectively.

OBJECTIVE: To establish the quality standards for Qudu capsules. METHODS: TLC was used to identify Rheum Palmatum, Phellodendron Amurense, Angelica Sinensis and Pheretima Aspergillum in Qudu capsules. HPLC was used to determine the content of the effective ingredient Paeonia Lactiflora. RESULTS: The spots characteristic of Rheum Palmatum, Phellodendron Amurense, Angelica Sinensis and Pheretima Aspergillum were found through detection by TLC. The linear range of Paeonia Lactiflora was 0. 094 4～ 0. 944 0? g( r=0. 999 8) . The average recovery was 99. 94% ( RSD=1. 74) . CONCLUSIONS: This standard can be used for the quality control of Qudu capsules.

Objective To investigate the expression and significance of p38 mitogen-activated protein kinase (p38MAPK) in hyperoxia-induced lung injury of new-born rats. Methods Totally 160 rats at the age of 12 h were randomly and equally divided into air control group, hyperoxia-induced lung injury group, hyperoxia-induced lung injury+SB203580 group and hyperoxia-induced lung injury + normal saline group. After the injury was inflicted, SB203580 or normal saline at same volume was given intraperitoneally at 5 mg/kg. After the rats were executed at the time points of 12, 24, 72 h and 1 week after the model establishment, the right upper lungs were resected for histopathology, right below lungs for wet weight/dry weight, and left lungs for detecting the expression of p38MAPK by Western blot analysis. Results Hyperoxia-induced lung injury model were established successfully after 72 h by exposure to hyperoxia. No p38MAPK expression was observed in air control group. In hyperoxia-induced lung injury group p38MAPK was detected from 12 h, reached to the peak at 72 h, and decreased 1 week later, and its expression was significantly higher in this group and normal saline group than in SB203580 group. Conclusion p38MAPK is involved in the process of hyperoxia-induced lung injury, and the injury can be relieved by treatment of SB203580.