2.Diagnosis And Treatment of Spontaneous Rupture of Primary Liver Cancer (Clinincal Analysis Of 20Cases
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
in this Article 20 Cases of Spontaneous rupture of primary liver Cancer Adimitted and treated in our hospital in the past 23 years (1956-1979) Are Analyed. of these 19 Were Male And 1 female, their Age ranged from 20 to 65 years, 75 per Cent Were 30 to 40 years of Age, The incidence Was 8 per Cent.Diagnosis, differential diagnosis and the mechanism of Spontaneous ruptnre of primary liver Cancer as Well as the Cause of Erroneous diagnosis are discussed in detail.The Clinical records revealed that 50 Per Cent of the Patients Who deid Within one month. After the rupture Should be reseued promptly, 2 Cases Survived 4 and 19 months respectively After the ligation of he patic artery, in order to prolong the Patients. life, it is Considered positive to excute ligation of hapatic Artery to stop bleeding And prevent the progress of Cancer.
3.Ultrastructure of mycardial cells and expression levels of tumor necrosis factor α in myocardium tissue of diabetic rabbits during early myocardial ischemia and their significances
Qingbin ZHAO ; Dandan LIU ; Chao LI
Journal of Jilin University(Medicine Edition) 2017;43(3):463-467
Objective:To investigate the changes of ultrastructure of myocardial cells and the expression levels of tumor necrosis factor α(TNF-α) in myocardium tissue of the diabetic rabbits during early myocardial ischemia and their mechanisms,and to provide the theoretical basis for studying the pathogenesis of diabetic myocardial ischemia injury.Methods:A total of 42 healthy male New Zealand white rabbits were chosen.The method of intravenous injection of alloxan in ear vein was used to establish the diabetic models in 26 rabbits among 42 rabbits;the method of partial ligation of left anterior descending coronary artery by thoracotomy was used to establish the diabetic myocardial ischemia models in 22 model rabbits with diabetes.A total of 16 (modeling succesfully) were divided into diabetic myocardial ischemia 7 d group and 28 d group;other 16 rabbits were used as sham operation groups at the relevant time points(7 and 28 d),and there were 8 rabbits in each group.The ultrastructure of myocardial cells was observed by transmission electron microscope;the apoptosis index (AI) of myocardial cells in ischemic myocardium tissue of the rabbits was detected by TUNEL method;the expression levels of TNF-α in ischemic myocardium tissue of the rabbits were detected by real-time RT-PCR method.Results:In sham operation groups,the nuclei of myocardial cells was bigger,the nuclear chromatin was in uniform distribution,the myofibril ordered arrangement,the sarcomere was neat;the mitochondria between myofibril were rich,and their structures were clear.For the rabbits in diabetic myocardial ischemia group,their early-stage cardiocyte nuclei were irregular in shape,myofibrils was loose in structure,the part of myofibril was broken,and the mitochondrial hyperplasia between the myocardial cells was obvious.Compared with sham operation groups,the AI of myocardial cells and the expression levels of TNF-α in the ischemic myocardial cells of the rabbits in diabetic myocardial ischemia 7 d and 28 d groups were significantly increased(P<0.05),and the indexes mentioned above in diabetic myocardial ischemia 7 d group was higher than those in diabetic myocardial ischemia 28 d group(P<0.05).Conclusion:The destruction of ultrasturctue and obvious apoptosis of the myocardial cells of the rabbits occur in the early stage of diabetic myocardial ischemia.The increasing of expression level of TNF-α may involve in the myocardial ischemic injury.
4.Prevention and treatment of normal peifusion pressure breakthrough in the treatment of cerebral arteriovenous malformation
Zhaohui LI ; Chao DU ; Xingli ZHAO
International Journal of Cerebrovascular Diseases 2009;17(12):918-921
Normal perfusion pressure breakthrough is a serious complication that may occur in the treatment of cerebral arteriovenous malformations. This article reviews the progress in research on normal perfusion pressure breakthrough in recent years. It mainly investigates its pathogeneses, predictive methods and prevention and treatment strategies.
5.Changes in epidemiology and clinical characteristics of cervical carcinoma over the past 50 years
Enfeng ZHAO ; Lei BAO ; Chao LI
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To evaluate the changes in the epidemiology and clinical characteristics of cervical carcinoma over the past 50 years, and to explore the equitable treatment corresponding to these changes. Methods The clinical and pathological data of 1557 patients with invasive cervical carcinoma from January 1955 to December 2004 were retrospectively analyzed. Results (1) The average age of cervical carcinoma onset gradually decreased over the past 50 years, from 56.27?8.45 in years 1955~1964 to 43.81?8.9 in years 1995~2004. At the same time, the radio of clinically early (stages Ⅰ-Ⅱ) and non-squamous cancer also steadily increased. (2) Young patients (≤35 year old) had higher ratios of non-squamous, poorly differentiated and late stage (Ⅲ-Ⅳ) cancers, with a higher rate of lymphatic metastasis compared with other ages. (3) Because of the changes in epidemiology and clinical characteristics of cervical carcinoma, it′s necessary to alter the traditional model of treatment and to explore equitable therapy corresponding to these changes. Conclusions The average age of cervical carcinoma onset gradually decreased and simultaneously the ratio of clinically early (stages Ⅰ-Ⅱ) and non-squamous cancer also steadily increased over the last 50 years. Patients aged 35 and younger of cervical carcinoma had some obvious clinical characteristics compared with that of older patients. Preservation of reproductive endocrine function ought to be deliberated on in cervical carcinoma treatment of women with fecundity. Neoadjuvant intraarterial chemotherapy (NAIC) is an useful method for cervical carcinoma at present
7.Perioperative Nursing of Sacral Neuromodulation for the Treatment of Neurogenic Bladder
Chinese Journal of Rehabilitation Theory and Practice 2006;12(12):1048-1049
To summarize the experience about the preoperative and postoperative nursing of 11 cases with neurogenic bladder treated by sacral neuromodulation. The main nursing interventions of this procedure involved accurate recording of voiding diaries and postvoid residual, effective psychological nursing and health education of related disease information, standard videourodynamic examination. 11 patients acquired more or less benefit after the screening test and were discharged successfully, among them 2 cases had the permanent implantation of the stimulation device.
8.Effect of decoy receptor-3 and glutamic acid decarboxylase-65 recombinant adenovirus on type 1 diabetes mice
Min ZHANG ; Shufa LI ; Bing CHEN ; Jianyong ZHAO ; Chao JIANC
Chinese Journal of Endocrinology and Metabolism 2012;28(2):150-152
The decoy receptor-3 ( DcR3 ) and glutamic acid decarboxylase-65 ( GAD65 ) recombinant adenovirus was construced and transduced into denlritic cells (DC). After the transduced DC were utilized to immunize NOD mice,the CD+8 T cells and blood glucose were analyzed. The results showed that recombinant adenovirus inhibited the proliferation and cytokine release of GAD65 specific T cells,and delayed the incidence of diabetes.Both interferon-γ[ (50.5±7.2)vs(95.4±6.9) and(91.2±6.5) pg/ml] and interleukin-2 [ (46.3±5.1 )vs ( 86.1 ±5.2 ) and ( 80.3 ± 7.3 ) pg/ml ] were decreased compared to those in negative and blank controls ( all P<0.05 ).The results suggest that DcR3 and GAD65 recombinant adenovirus might provide a promising way for gene therapy of type 1 diabetes.
9.Effects of exenatide on the cell proliferation, invasion and apoptosis of human tongue squamous cell carcinoma SCC-25
Chao HUANG ; Feifei SHEN ; Gang LI ; Yue ZHAO
Tianjin Medical Journal 2015;43(5):461-464
Objective To detect the effects of exenatide on the related indicators of proliferation, invasion and apoptosis of cell line SCC-25. Methods SCC-25 cells were cultured in vitro. The expression level of glucagon like peptide 1 receptor (GLP-1R) was determined by Western blot assay in SCC-25 cells. SCC-25 cells were divided into four groups:control group and exenatide group (1,10 and 100 nmol/L). The ability of cell proliferation was detected using MTT assay after 24 h, 48 h and 72 h of culture. The ability of invasion was measured with Transwell assays. The expression levels of MMP-2, Caspase-3 and Phospho-p38 MAPK were measured by Western blot assay. Results GLP-1 receptor expression was found in SCC-25 cells. Compared with control group, the cell survival rate, invasion rate and the expression of MMP-2 were significantly decreased in SCC-25 group (P<0.05). The expression of Caspase-3 were significantly increased (P<0.05).Changes were in a concentration-dependent and time-dependent manner (P<0.05). The expression of Phospho-p38 MAPK was significantly increased at 24 h in 10 nmol/L exenatide group (P<0.05). Conclusion Exenatide can inhibit the cell proliferation and invasion, which may contribute the apoptosis by promoting expressions of Phospho-p38 MAPK and Caspase-3 of SCC-25 cells.
10.Cytokine-induced killer cells specifically inhibits implanted gastric cancer cells
Chao LIU ; Weizheng MAO ; Baocheng ZHAO ; Hui LI
Chinese Journal of Cancer Biotherapy 1995;0(03):-
Objective:To investigate the inhibitory effect of cytokine-induced killer cells (CIK) against implanted gastric cancer cells. Methods:Gastric cancer SGC-7901 cells were subcutaneously injected into the inguina of nude mice to establish gastric cancer model. The tumor bearing mice were randomly divided into CIK group and fibroblasts group,in which mice were subcutaneously injected with fluorescence dye SP-DiI labeled CIK and fibroblasts HFL-I cells,respectively. Distribution of CIK and HFL-I cells in different tissues of gastric cancer bearing mice were observed. Meanwhile,tumor volume was measured after different treatments and tumor inhibitory rate was calculated. Tumor necrosis areas in different groups were observed. Results:SP-DiI labeled CIK was mainly located in the gastric cancer tissues 10 d after injection,and was hardly detected at the injection sites,liver,spleen and lung tissues (P