Sanger sequencing technology is the most commonly used method for genetic analysis in inherited eye disease in the past few decades on account of its long DNA sequencing read length and high accuracy.However,application of this method is limited in genetically heterogeneous diseases because of the high economic and time cost.Next generation sequencing (NGS) technology is a high-throughput,cost-effective,and highly automated method which has been widely used since 2005.Although NGS is especially suitable for studies on genetically heterogeneous inherited eye disease,overdependence on the technique itself and neglect of the assessment of the phenotype may lead to misjudgment of the testing results and higher economic burden for the patients.So far,the most common method on researches of monogenic inherited eye disease is to combine NGS and Sanger sequencing technique.Precise evaluation of the clinical phenotype of patients is very important,as it is related with selection of detection methods and determination of disease-causing mutations.

Paeoniflorin in Chinese traditional patent medicine "Moluo granules" was determined byreversed phase HPLC.Optimum separation was achieved by employing C18 column as the stationary phase,MeOH-(CH3)2 CHOH-36% HOAc-H2O(25∶2∶2∶71) as the mobile phase.Theaverage recovery is 99.06%,and the coefficient of variation is 1.04%,The extraction is simple,the analytical method is rapid and accurate with good reproducibility.

To introduce the academic thoughts of traditional Chinese medicine(TCM)master GUO Zi-guang based on the analysis of clinical cases.His main academic thoughts are:①He proposes the theory of six meridians and their differentiation in the diagnosis and treatment of typhoid.The six meridians are bases for the treatment of all diseases,and their differentiation provides a fundamental principle for the differentiation of diseases.②He uses the method of combining the disease and syndrome in diagnosis and treatment,which widens the horizon of traditional differentiation and diagnosis in TCM and avoids the absence of syndrome in the practice of differentiation.In this way,the therapeutic effects of Chinese medicine can be guaranteed so as to meet the needs of medical and social development.③He emphasizes that it is fundamental to improve the therapeutic effects of TCM for its development.The approach is to combine disease and syndrome in diagnosis and treatment,and the way is to apply different methods in disease treatment.

Objective:To assess the anatomy of portal vein and hepatic vein with three dimensional 16-MSCT angiography.Methods:A total of 100 healthy subjects underwent upper abdominal MSCT examinations in our department.All subjects were administered 90～120ml of IV contrast material at an injection rate of 3ml/s.The scan delay time was 45～60s and 90～120s for portal venous and hepatic venous phase with automated bolus tracking,respectively.All data sets were reconstructed in 1-mm section and 0.5mm interval,and then were transferred to a workstation for maximum intensity projection(MIP)and volume ren-dering(VR) econstruction.The portal vein anatomy was classified according to Couinaud and Atri’s classification;the hepatic veinanatomy according to Nakamura’s,Marcos’s and Kawasaki’s classification.Results:① Normal portal vein anatomy was seen in 76 cases,type Ⅱin 16,type Ⅲ in 6,type Ⅳ in 2.②Nakamura’s TypeⅠof hepatic vein was presented in 72 cases;type Ⅱ in 22.type Ⅲ in 6.The branching pattern of the middle hepatic vein was type Ⅰin 65 cases,type Ⅱin 19,and type Ⅲ in 16.Segment IVb vein branched from the middle hepatic vein in 42 cases,and from the left hepatic vein in 58.Conclusion:3D 16-MSCTA is a significantly valuable tool which can provide complete and comprehensive information on the anatomy of portal vein and hepatic vein combined with MIP and VR image.

Objective: To study the effects and significances of lipopolysaccharide preconditioning on the activities of NF-?B and the expression of ICAM-1/LFA-1 in rats graft liver ischemia/reperfusion injury. Methods: Male Sprague-Dawley rats were divided into three groups: sham operation group(Sham group),orthotopic liver transplantation group (OLT group) and LPS preconditioning group (LPS group). Only dissecting hepatoduodenal ligament was perfomed in Sham group. Experiments of OLT were performed by two-cuff method in OLT group and LPS group. The activities of NF-?B and the expression of ICAM-1/LFA-1 in hepatic tissue ,the levels of ALT,AST in inferior caval vein blood were detected at 0,60,180 min after dissecting of hepatoduodenal ligament in Sham group and after portal vein reperfusion in OLT group and LPS group. Results: Compared with those in Sham group at the different time points respectively,the activities of NF-?B and the expression of ICAM-1/LFA-1 were higher in OLT group and LPS group(P0.05) at 0 min after reperfusion,they were evidently higher in OLT group than in LPS group (P

Objective:To culture mesenchymal progenitor cells(MPC)from compact bone fragments in C57 mice in vitro and to study the feasibility of inducing directed differentiation of MPC into neuron-like cells in vitro. Methods:Bones of hind limbs of C57 mice were sheared into bone fragments and digested by collagenase type Ⅱ. Then,MPC were cultured in vitro and analyzed by flow cytometry for identification of its immunology phenotype. MPC of P3 in good growth were induced directionally by the supernatant cultured with primary neuron,and then detected the expression of neuronal specific markers neuron specific enolase(NSE)and neurofilament protein(NF)by immunocytochemical staining. Results:The primary MPC were cultured successfully and they grewwell after passage. Compared with that of the control group,the positive rate of CD29 and CD44 of MPC of antibody group had significant difference (P

Objective To compare of treatment effects of intravenous iron and oral iron on hemodialysis ane- mia in patients with erythropoietin.Methods The oral dosage of ferrous succinate was 200mg dose,3 times a day for 2 months.After washing out for 2 months.Then these patients were given intravenous iron dextran with a dosage of 100mg twice a week for 5 weeks.During the treatment,hemoglobin (Hb),hematocrit (Hct),serum ferritin (SF) and trasferrin saturation(TSAT) were monitored.Results The efficacy of oral iron was 20 % ,the efficacy of intra- venous iron was 60 %.The Hb,Hct,SF and TSATS level increased obviously in the efficacy of intravenous iron,sta- tistical analysis showed a very significant difference(P

Objective:To investigate the effects of endoscopic local treatment for colorectal intramucosal carcinoma.Methods:42 patients with colorectal intramucosal carcinoma were treated with endoscopic local through one or few methods such as biopsy forceps resection,looping ligature and high frequency electric coagulation etc.in past five years.Results:(1)18 cases received biopsy forceps resection,3 cases received looping ligature,16cases received high frequency electric coagulatio resection,5 cases received many kinds of methods.(2)3 cases of 5 cases with advanced carcinoma were operated enlarge radical surgery except 1 cases who refused operation and 1 cases who losted operation opportunity.In the rest 37 cases,7cases were operated surgery,malignant remnants were no founded in theses colon specimens.(3)The following-up 33cases(30 cases without surgery)failed to show recurrent cancer for 1 month to 5 years.(4)In the following-up cases,the incidence of neogenesis adenoma was 57.1% in cancer with pilosity adenoma and was 15.8% in early cancer without adenoma during 1 year to 3 years after operation.Conclusions:It can achieve to healing,security and minimum invasive treatment for endoscopic local resection to colorectal intramucosal carcinoma as long as it is suitable to select case.

Objective:To evaluate the influence of health education on knowledge and attitude about drugs and addictive drug abuse prevention among the junior middle school students.Methods:389 junior middle school students were selected in this program.Drugs and addictive drug abuse prevention related knowledge and attitude of the junior middle school students were surveyed by questionnaire anonymously before and after the health education.Results:After education,average score of drugs and addictive drug abuse prevention knowledge among the junior middle school students was significantly increased from 8.8 to 15.7(full score 17),suggesting the significant difference.The junior middle school student's level of knowledge about the danger of smoking and drinking also increased significantly.The correct rate of the answers rose from 4.6%～78.6% to 79.4%～100.0%.After this education,the rate of correct attitude about the drugs and addictive drug abuse prevention was significantly higher than before.Conclusion:The study suggests that health education on drugs and addictive drug abuse prevention among the junior middle school students has the positive impact on increasing the basic knowledge on the drugs and addictive drug abuse prevention for the junior middle school students as well as setting up the correct attitude on it.

Objective:To prepare levofloxacin-carboxymethyl-chitosan(LVFX/CMC) microspheres and to study their colon-targeted release in rats.Methods: KromasilRKR100-5C18(250 mm?4.6 mm)was used as the analytical column and the temperature was maintained at 50℃.The mobile phase consisted of a mixture of acetonitrile:0.1% trifluoroacetic acid(2080) pumped at a flow rate of 1.0 ml/min,with ?_(ex)295 nm and ?_(em)495 nm.Sixty healthy male SD rats were randomized into 2 groups: to receive gastric lavage with normal LVFX(40 mg,control group) and LVFX/CMC microspheres(40 mg,treatment group).HPLC was used to detect the concentrations of LVFX in the stomach,intestine,cecum,colon and blood in rats in both groups.Results: LVFX was detectable 3 h after LVFX/CMC microsphere lavage.Five hours after lavage,the levels of LVFX in cecum and colon were respectively(3.394?0.197) mg and(1.873?0.216) mg in treatment group,and(0.489?0.123) mg and((0.078?0.002)) mg in control group(P