Objective If IL-2 and IFN-? are united,perhaps we may see a more significant antitumor effect.A fusion gene of IL-2 and IFN-? was designed and constructed by DNA engineering.Methods CEA promotor、IL-2cDNA and IFN-?cDNA were obtained by PCR and RT-PCR.SDS-PAGE was used to examine the results;pcDNA-IL-2、pcDNA-IFN-?、pCEA-IL-2/ IFN-?、pEGFP-IL-2/ IFN-? were transfected to CEA high expression cell line(Lovo)by use of liposome.The fusion protein expression was examined by fluorescence microscope and Western blotting.Results IL-2/ IFN-? fusion gene can be expressed in tumor cells.Its expression is more obvious in CEA high expressed cells(Lovo),and can be located in cell plasma.Conclusion The fusion gene can be expressed in plasma of CEA high expressed cell lines.It may have a new anti-tumor effects.

Orthodontic tooth movement (OTM) is a very complicate mechano-biological procedure which is accomplished by the remodeling of periodontal tissues via orthodontic force.As the connective tissue between alveolar bone and cementum,periodontal ligament plays a vital role in the response and conduction of orthodontic force.The susceptible response of periodontal ligament to orthodontic force during OTM is a critical factor on inducing and modulating periodontal tissue restitution.This article reviews the concrete role of periodontal ligament and its mechano-biological behavior during OTM,discusses its mechanical response and biological mechanism respectively on histological,cellular and moleucular levels.

Reading therapy in hospital libraries, academic libraries and public libraries was described in terms of its theoretical study and practical study,and its significance in hospital library was elaborated by comparative analysis.

Objective:To investigate the clinical efficacy of loratadine combined with compositus mentholi cream in the treatment of infantile with external ear eczema and evaluate the safety .Methods:Totally 96 cases of infantile with external ear eczema were ran-domly divided into the observation group (n=48) and the control group (n=48) according to a random number table .Both groups were given the conventional treatment .The control group was treated with topical compositus mentholi cream , while the observation group was treated with loratadine additionally .Both groups were treated for 10 d.The clinical efficacy , the level changes of interleukin-4 (IL-4), interferon-γ(IFN-γ) and immunoglobulin-E (IgE) before and after the treatment, the incidence of adverse reactions during the treatment and the relapse after 6-month follow-up were compared between the groups .Results:The total effective rate of the obser-vation group (95.83%) was significantly higher than that of the control group (79.17%, P<0.05); the levels of IL-4, IFN-γand IgE in both groups were significantly decreased after the treatment (P<0.05), and those in the observation group were significantly lower than those in the control group (P<0.05);there was no significant difference in the incidence of adverse reactions between the groups (P<0.05); the recurrence rate in the observation group (8.33%) was significantly lower than that in the control group (22.92%, P<0.05).Conclusion:The clinical efficacy of loratadine combined with compositus mentholi cream in the treatment of infantile with external ear eczema is reliable with promising safety .

The WeChat service provided at present in 14 hospitals directly under the Yunnan Health and Family Planning Commission was surveyed with suggestions proposed for providing active Wechat service in hospitals in order to alleviate the difficulty to see a doctor and the tense relationship between doctors and patients.

Functional gastrointestinal diseases(FGIDs) mainly include functional dyspepsia (FD),irritable bowel syndrome (IBS),functional constipation (FC) and functional abdominal pain syndrome (FAPS).In gastroenterology outpatient,42%～61% FGIDs patients have psychological factors,mostly depression and anxiety.The personality of these patients are almost introvert.Negative life events can be an incentive to lead to depression and anxiety which can aggravate the functional gastrointestinal symptoms.Clinical manifestations include long-term abdominal discomfort,abdominal pain (some with no fixed position),abdominal distension,anorexia,change in bowel habits,retrosternal and upper abdominal burning pain or overlapping symptoms.Some have obvious emotional performance.Dyspepsia symptom score of FD patients with mental disorders are higher than those who have no emotional performance and the gastric emptying decrease greatly than the latter.FGIDs treated by systemic therapy but with no improvement in symptoms may have somatization,compulsion,interpersonal tension,depression,anxiety and other psychological disorders.So psychological evaluation should be carried out to make early recognition for refractory functional gastrointestinal disorders.

TCM has dominance and characteristics in treating chronic nephritis, such as protecting the function of kidney and defering the kidney crocking up. Professor LI Ji-ren, a famous herbalist and the gainer of the special allowance of the State Council, has worked on clinic for 60 years. He Accumulates abundant experiences in treating chronic nephritis. On clinic, Professor Li advocates integratation of syndrome differentiation and disease differentiation, attaching importance to protect the basic of body and flexibility of syndrome differentiation. In aspects of medication, he begins with treating spleen and kidney,at the same time, harmonizing the qi and blood, detaching the clear and dirtiness, protecting other organs. Danbai Zhuanyin Prescription, containing thirteen herbal medicines, has marked effect on chronic nephritis albuminutia.

To explore the stability of impact assay for determination of microorganism in indoor air, the comparisons of continued sampling times of 2-minute and 4-minute for monitoring on microorganism of indoor air were carried out with JWL- Ⅱ B multifunctiom air microorganison monitor in 11 hotels, public places of entertainment, barbar shops and beauty shops. The results showed that, under a same environmental condition, there was no significant differences between the data obtained by 2-minute monitoring and 4-minute monitoring (t=1.854, P＞0.05).It indicated that impact assay presented very good stability in detecting the microorganism of indoor air within certaindetecting duration.

Since cancer-related pain is the worldwide problem,intrathecal drug delivery system (IDD)has been an effective pain management option for patients with chronic and cancer pain.Compared with the other ways,such as oral or intravenous infusion,the delivery of drugs into the intrathecal space provides superior analgesia with lower doses of analgesics to minimize side effects while significantly improve quality of life.Recently,besides traditional opioid (especially mainly morphine) for intrathecal,clinical experts have also found some other drugs could be used for IDD to control the cancer-related pain,and have observed their effect and related adverses.This article aims to provide a general overview of the development trend in this field.

Objective To study the correlation of blood uric acid,lipoprotein associated phospholipase A2(Lp-PLA2) and arterial stiffness in young patients with coronary heart disease.Methods 56 young patients with coronary heart disease were selected as the observation group,50 young healthy subjects were selected as control group.The serum uric acid,Lp-PLA2 and arterial stiffness in the two groups were determined.Blood uric acid,Lp-PLA2 and arterial stiffness in patients with coronary heart disease were analyzed.Results There were no significant differences in age,sex,BMI,systolic blood pressure,diastolic blood pressure and triglyceride between the two groups (all P>0.05).The total cholesterol,low density lipoprotein,uric acid and Lp-PLA2 in the observation group were significantly higher than those in the control group (t=3.927 4,4.162 4,5.772 6,9.770 1,P<0.05,P<0.01).High density lipoprotein in the observation group was significantly lower than that in the control group (t=2.6043,P<0.05).Multivariate Logistic regression analysis showed that low density lipoprotein cholesterol,uric acid,Lp-PLA2 were independent risk factors for coronary heart disease (low density lipoprotein uric acid OR=1.053,95% CI:1.031-1.106;uric acid OR=1.082,95% CI:1.043-1.115;Lp-PLA2 OR=1.007,95% CI:1.002-1.011).The arterial stiffness of the observation group was (8.86±1.63)v·cm-1·s-1,and had positive correlation with serum uric acid (r=0.221,P<0.05) and Lp-PLA2 (r=0.361,P<0.05).Conclusion Blood uric acid,Lp-PLA2 can reflect the early arterial stiffness in young patients with coronary heart disease.