0.05).The positive expression rate of CXCR4 was 96% in cancer cells in regional metastasized lymph nodes.And its expression was lower than that in primary tumors(P

Objective:To make the isolation and 2-DE analysis of mitochondria,in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation,and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable,which provides a basis for the following proteomic study on mitochondria.

0.05);The levels of basal FINS showed a significant differences between group A and group B(P0.05).Conclusions Intensive insulin treatment to newly diagnosed type 2 diabetes patients can control blood glucose rapidly to a desired level,and can recover the function of B cell better than traditional oral hypoglycemic agents by dissolving the virulence of glucose rapidly.

Objective: To study the effect of Resen heche tablets (RSHC) on tonyfing and warming Shen Yang. Methods: The mice model of Shen Yang deficiency was established by injecting hydrocortisone. The body weight, autonomic activity and swimming time in ice water in model mice were observed. The level of superoxide dismutase (SOD) in red cell and lipid peroxide (LPO) in plasma of aged rats were analyzed. The effect on anti stress in mice was examined. Results: RSHC had the remarkable effect to improve body weight and autonomic activity and to prolong swimming time in ice water in model mice, and to increase SOD activity and reduce LPO in blood of aged rats, and to elevate the ability of anti fatigue and anoxic tolerance in mice. Conclusion: RSHC drug has the effect on tonifying and warming Shen Yang; can promote growth, enhance organism activity, elevate the adaptation to circumstances and protect injury from free radicals.

Objective The study aimed to explore the reliability and validity of the Chinese version of the Nottingham Assessment of Functional Footcare (NAFF) in investigating the footcare behavior's level in patients with high risk of diabetic foot.Methods According to Bradley's principle,NAFF was converted into the Chinese version scale,then it was applied to 211 cases of patients with high risk of type 2 diabetic foot,and the reliability and validity of foot care be-havior scores were analyzed.Results In the Chinese version scale,the Cronbach's alpha coefficient was 0.77,test-retest reliability was 0.74.Content validity,ICVI was 0.80 to 1.00,S-CVI/Ave was 0.96,and the adjusted value of kappa was 0.75 to 1.00.Conclusions The questionnaire has been tested to have good reliability and validity.The NAFF possesses certain value in the clinical application and could be suitable for Chinese patients with high risk of type 2 diabetic foot.

The doctor-patient relationship is always the focus in the society. The relationship seems more complex and sensitive in the pediatric surgical ward because its patients are kids. However, the pediatric surgical residents' ability of doctor-patient communication is lack of systemic and norma-tive training, which is not good for the mutual understanding between doctors and patients. This study explores how to enhance the residents' doctor-patient communication skills from the perspective of awareness, professional knowledge and some specific skills. In the meantime, it discusses the need to train the teachers and set up a regular examination-assessment system.

Virus is a kind of pathogen which has greatly threat-ened human lives and is severely harmful to human’ s health. It is featured by rapid transmission and high mortality. At present there is no efficacious anti-virus western medicine. Some kinds of traditional Chinese medicines show certain restrictive effect a-gainst virus as natural materials and become a hot spot. This pa-per proceeds from experimental study on anti-virus activity and action mechanism of febrifuge and detoxifying herbs, and eluci-dates the important role of febrifuge and detoxifying herbs in anti-virus effect to provide evidence for screening anti-virus Chinese medicine in clinic.

The expression of c-met stimulated by high glucose in human renal tubular epithelial cells and the role of HGF/c-met system in diabetic nephropathy were examined. The proximal tubular epithelial cells were cultured in vitro under different conditions. MTT was used for the detection of cellular proliferation and RT-PCR was employed for measurement of c-met mRNA level. Our results showed that under different conditions, there were no significant differences in the proliferation of proximal tubular epithelial cells 12 h and 24 h after the culuture (P>0.05). The proliferation of proximal tubular epithelial cells showed a significant change 96 h after the culture and the cellular proliferation induced by hepatocyte growth factor (HGF) was very active (P<0.05). Moreover, no significant difference in the expression of c-met mRNA was found 12 h after the culture under different conditions (P>0.05), while 24 and 96 h after the culture, a persistent and significantly higher expression of c-met mRNA was found in HGF-induced proliferation. It is concluded that addition of exogenous HGF could inhibit the apoptosis induced by high-level glucose, promote the proliferation of proximal tubular epithelial cells, and induce the expression of c-met. Our study suggests that local up-regulation of HGF/c-met system plays an important role in the repair of renal damage in diabetic nephropathy.

Objective To investigate the expression of activator protein-1 (AP-1) in PBMCs from patients with dermatomyositis (DM) and glucocorticoid effect on the expression.Methods PBMCs were isolated from 15 normal human controls and 20 patients (including 12 patients with primary DM who had never received glucocorticoid treatment and 8 patients with recurrent DM who had stopped glucocorticoid treatment for more than 1 month),and classified into two parts:one was cultured in RPMI 1640 medium with dexamethasone of 80 μmol/L and 10％ calf serum for 48 hours,and the other was frozen at -80 ℃ and reserved.Electrophoretic mobility shift assay (EMSA) was carried out to detect the activity of AP-1 in these cells.Results The expression (grayscale area) of AP-1 in the PBMCs from the normal controls was 4.93 ± 0.15 mm2.A significant decrease was induced by the treatment with dexamethasone in the expression (grayscale area) of AP-1 from PBMCs of patients with primary DM and those with recurrent DM (5.59 ± 0.39 vs.30.23 ± 0.49 mm2,5.69 ± 0.39 vs.34.79 ± 0.61 mm2,F =13812.64,P ＜ 0.01 ).The activity of AP-1 was statistically higher in the PBMCs from patients with recurrent DM than in those from patients with primary DM before the treatment with dexamethasone (P ＜ 0.01 ).Conclusions The enhanced activity of AP-1 may be an important factor inducing the inflammatory reaction in and recurrence of DM.Glucocorticoids may suppress the AP-1 activity to a certain degree.

The pathological changes in myocardial cell of diabetic rats were consistent with diabetic cardiomyopathy. The levels of adiponectin and the expression of T-cadherin in diabetic group were increased gradually,and higher than in normal control group