Objective To explore the relationship of urinary iodine level between 8-10 year-old children and adults under the same living conditions in coastal and mountain areas in Fujian Province.Methods Representative coastal and mountainous rural areas (Xindian Town Xiang'an District Xiamen City and Yongfu Town Zhangping District Longyan City) were selected as survey sites.Morning fasting urine samples from local 8-10 year-old children and their parents and a random urine sample in the morning from these children were collected.Urinary iodine was determined using the arsenic cerium catalytic spectrophotometric method (WS/T 107-2006).Results The median urinary iodine of fasting urine samples which were collected from 147 8-10 year-old children in coastal rural areas (71 boys and 76 girls) was 163.5 μg/L (boys 161.0 μg/L,girls 170.9 μg/L),and the median urinary iodine of random urine samples was 190.8 μg/L (boys 208.7 μg/L,girls 177.0 μg/L),and the difference was not statistically significant between boys and girls (Z =-0.376,-0.678,all P ＞ 0.05).The median urinary iodine of fasting urine samples which were collected from 116 8-10 year-old children in mountain rural areas (66 boys and 50 girls) was 171.8 μg/L (boys 169.5 μg/L,girls 173.8 μg/L),and the median urinary iodine of random urine samples was 210.8 μg/L (boys 233.2 μg/L,girls 203.4 μg/L),and the difference was not statistically significant between boys and girls (Z =-0.413,-0.881,all P ＞ 0.05).The median urinary iodine of random urine samples which were collected from 8-10 year-old children in mountain rural areas was significantly higher than that of the morning fasting urine samples (Z =-2.789,P ＜ 0.05).The median urinary iodine of children's fathers in coastal rural areas was 177.0 μg/L,and the median urinary iodine of their mothers was 181.7 μg/L.There was no significant difference between coastal rural children and their parents (Z =-0.387,-0.658,all P ＞ 0.05),and there was also no significant difference between fathers and mothers (Z =-0.014,P ＞ 0.05).The median urinary iodine of children's fathers in mountain rural areas was 227.3 μg/L,and the median urinary iodine of their mothers was 237.4 μg/L There was a significant difference between mountain rural children and their parents (Z =-4.176,-4.373,all P ＜ 0.05),and there was no significant difference between fathers and mothers (Z =-0.291,P ＞ 0.05).Conclusions Urinary iodine level of school-age children does not reflect the actual urinary iodine level of adults.There is a difference between urinary iodine level of random urine sample and morning fasting urine sample.Attention should be paid to this difference when monitoring iodine nutrition in population.

Objective To investigate the changes of excitatory amino acids of cerebrospinal fluid(CSF) in patients with acute cerebral infarction(ACI) and its clinical significance.Methods The CSF levels of excitatory amino acids including glutamate(Glu) and aspartate(Asp) in 30 cases with ACI and 20 control subjects with migraine were measured by high performance liquid chromatography.Results Glu and Asp levels in patients with ACI were significantly higher than those of the controls(all (P

To establish a method for determination contents of schizandrin, tanshinone I, cryptotanshinone, tanshinone II (A) and schizandrin B in rongxin pills. The HPLC method was performed on an Agilent C18. The mobile phase was composed of methnol and water wish gradient elution. The flow rate was 1.0 mL x min(-1). The column temperature was 30 degrees C and the detection wavelength wash 240 nm. The linear of schizandrin, tanshinone I, cryptotanshinone, Tanshinone II (A) and schizandrin B were 3.000-48.00 (r = 1.000), 3.985-63.76 (r = 0.999 9), 6.370-101.9 (r = 1.000), 8.690-139.0 (r = 0.999 9), 1.700-27.20 mg x L(-1) (r = 0.999 9), respectively. The average recoveries were 98.44%, 100.3%, 99.29%, 99.07%, 98.42%, and RSDs were 0.61%, 1.1%, 0.52%, 0.72%, 0.97%. The method is convenient, accurate and has good precision. It can be used for determination of the preparation.
Chromatography, High Pressure Liquid ; Cyclooctanes ; analysis ; Diterpenes, Abietane ; analysis ; Drugs, Chinese Herbal ; chemistry ; Lignans ; analysis ; Linear Models ; Organic Chemicals ; analysis ; Phenanthrenes ; analysis ; Polycyclic Compounds ; analysis ; Quality Control ; Reproducibility of Results

Objective Using Beckman assay kit to establish women thyroid function indicators reference interval during pregnancy in Fujian Province.Methods Two iodine sufficient areas were selected in Fujian randomly,and the women who did pregnancy test in local hospital were investigated.A questionnaire study and physical examination were conducted and women's serum samples were collected to detect the thyroid function [thyroid stimulating hormone (TSH),free triiodothyronine (FT3),free thyroxine (FT4),total triiodothyronine (TT3),and total thyroxine (TT4)] using Beckman chemiluminescence analyzer access-2 and assay kit.Reference interval was shown with median (M) and P2.5-P97.5.At the same time,the newly established reference interval was used to evaluate the thyroid function of 830 pregnant women.Result A total of 455 pregnant women were detected (early,middle and late pregnancies were 146,157 and 152,respectively).Most of the thyroid function indexes presented skewed distribution.The thyroid function index reference intervals in early,middle and late pregnancies were TSH:0.80 (0.02-2.96),1.13 (0.07-3.56),1.18 (0.09-3.76) mU/L;FT3:4.28 (3.30-5.76),3.74 (2.78-4.83),3.63 (2.81-4.56) pmol/L;FT4:11.78 (8.66-19.68),9.34 (7.03-12.76),8.55 (6.20-11.73) pmol/L;TT3:1.91 (1.09-2.95),2.00 (0.90-2.96),1.98 (0.91-3.02) nmol/L;and T4:123.00 (79.62-194.85),115.42 (58.78-175.85),117.43 (58.29-192.00) nmol/L.The thyroid dysfunction rate diag,nosed by our reference interval was 22.0％ (183/830),whilch was lower than 28.0％ (232/830) diagnosed by American Thyroid Association (ATA) reference interval (x2 =7.714,P ＜ 0.05).Conclusions Women thyroid function indicators reference interval during pregnancy in Fujian Province is successfully established.The study shows that each area and laboratory should establish the thyroid hormone reference interval of pregnant women for diagnosis and treatment of thyroid disease.

Objective To evaluate the influence of duration of laser irradiation on histomorphometric meas- urements in experimentally tenotomized and repaired rabbit Achilles tendons and to explore the best irradiation time. Methods A total of 20 male New Zealand rabbits aged 10-12 weeks were used and randomly divided into 4 groups:a control group and three experimental groups.All the animals underwent surgical excised and then repair of their Achillis tendon.The animals in the control group were then treated with sham laser irradiation,while those in the three experimental groups were treated with 10,20 and 30 minutes of He-Ne laser irradiation(632.8 nm, 18.9 mW)daily,respectively,for 14 days.On the 28th day after surgical operation,the animals were sacrificed and their Achilles tendons were sampled.HE stain and Van Geison stain were used to observe morphometric changes of tendons.The SDS-PAGE electrophoresis method and CS-930 photodensity scan instrument were employed to measure the content of typesⅠandⅢcollagen.Results it was shown that laser irradiation enhanced cell proliferation,cel- lular content,granulation tissue formation and collagen deposition in laser-treated tendons,especially in those irradia- ted for 20 minute daily,as compared to the control group.TypeⅠand typeⅢcollagen levels were significantly in- creased at the 28th day in the healing tendons and the ratio of collagenⅢtoⅠincreased in all the 3 experimental groups,and the increase of both collagen content and ratio of collagen typeⅢtoⅠwas significantly greater in those ir- radiated 20 minutes daily(P

Abstract? AlM: To explore the effect of Danshen injection iontophoresis combined with lumbrukinase on early patients with retinal vein occlusion ( RVO ) , and observe the prognosis to provide objective basis for clinical?METHODS: Eighty-two patients ( 117 eyes ) with early RVO were randomly selected in our hospital from January, 2011 to April, 2014, 41 cases (59 eyes) of control group treated with Lumbrokinase. Forty-one cases ( 58 eyes) of the observation group, treated with Danshen injection iontophoresis combined with lumbrokinase. The main indexes were the treatment effect, visual acuity and retinal circulation time.?RESULTS: The total effective rate of the observation group was 85. 4% ( 35/41 ), more than that of control group 68. 3% ( 28/41 ), the difference was statistically significant (P<0. 05). The invalid rate of the observation group was 14. 6% (6/41), lower than that of the control group 31. 7% ( 13/41 ), the difference was statistically significant (P<0. 05). The vision of the observation group 4. 8 ~5. 0 was 53. 7% (22/41), more than that of control group 41. 5% ( 17/41 ), the difference was statistically significant ( P < 0. 05 ). The restore vision of the observation group 4. 3 ~ 4. 7 was 31. 7% (13/41), lower than that of the control group 41. 5% ( 17/41 ), the difference was statistically significant ( P<0. 05 ). After treatment, retinal circulation time of two groups were lower than before treatment, the difference was statistically significant (P<0. 05). After treatment of retinal vein circulation time of the observation group was 8. 15 ± 1. 30s, 9. 70 ± 1. 28s lower than that of the control group, the difference was statistically significant (P<0. 05).?CONCLUSlON: There is better effective on Danshen injection iontophoresis combined with Lumbrokinase for early retinal vein occlusion, and can improve the patient's visual acuity, promote the rehabilitation of patients.

Adult ; Atropine ; therapeutic use ; Bronchodilator Agents ; therapeutic use ; Combined Modality Therapy ; Female ; Hemofiltration ; Humans ; Insecticides ; poisoning ; Male ; Middle Aged ; Organophosphorus Compounds ; Poisoning ; therapy ; Treatment Outcome

The clinical efficacy and safety of auricular acupuncture (AA) for treatment of primary insomnia was evaluated. After a comprehensive retrieval in domestic and foreign databases, literatures were strictly screened and Revman 5.2 software was applied to perform a Meta-analysis on eligible randomized controlled trials (RCTs). The evidence quality was assessed with GRADE profiler 3.6 software. As a result, 8 articles were included involving 894 patients. Compared among AA and sham AA, placebo AA, blank control, there was significant difference in Pittsburgh sleep quality index (PSQI) [WMD = -3.48, 95% CI (-3.96, -3.00)], sleep latency LWMD = -10.14, 95% CI (-17.16, -3.12)] and sleep awakening times [WMD = -9.98, 95% CI (-1.10,-0.48)]. Compared between AA and western medication, there was significant difference in PSQI [WMD = -3.62, 95% CI (-4.59, -2.65)]. The evidence quality was moderate in AA vs. sham AA, placebo AA or blank control, while that of the rest was extremely low. No reports of adverse events were described in all studies. In conclusion, for the treatment of primary insomnia, AA could effectively improve sleep quality, but due to the low evidence quality, cautious attitude should be taken on this conclusion, and clinical trials with large sample and high quality were needed in the further.
Acupuncture, Ear ; Humans ; Randomized Controlled Trials as Topic ; Sleep ; Sleep Initiation and Maintenance Disorders ; physiopathology ; therapy

OBJECTIVETo develop a new technique for assessing the risk of birth defects, which are a major cause of infant mortality and disability in many parts of the world.

METHODSThe region of interest in this study was Heshun County, the county in China with the highest rate of neural tube defects (NTDs). A hybrid particle swarm optimization/ant colony optimization (PSO/ACO) algorithm was used to quantify the probability of NTDs occurring at villages with no births. The hybrid PSO/ACO algorithm is a form of artificial intelligence adapted for hierarchical classification. It is a powerful technique for modeling complex problems involving impacts of causes.

RESULTSThe algorithm was easy to apply, with the accuracy of the results being 69.5%±7.02% at the 95% confidence level.

CONCLUSIONThe proposed method is simple to apply, has acceptable fault tolerance, and greatly enhances the accuracy of calculations.

Algorithms ; Artificial Intelligence ; China ; epidemiology ; Environmental Exposure ; adverse effects ; Humans ; Infant, Newborn ; Models, Biological ; Neural Tube Defects ; epidemiology ; Risk Factors

This study is designed to obtain recombinant human acetylcholinesterase (rhAChE) and apply it in screening acetylcholinesterase inhibitors. The rhAChE was overexpressed in HEK293 cells transfected by plasmid of pCMV-AChE with the cationic liposome and rhAChE was found to be secreted into cell culture medium. AChE activity was assayed according to modified Ellman method to obtain kinetic parameters. IC so50 values for donepezil compounds of rhAChE were calculated to determine their activities of inhibition. The results showed that Km value was 151.9 micromol.L-1 donepezil inhibited rhAChE in a mixed competitive-noncompetitive way (Ki= 16.03 nmol.L-1, Ki = 18.36 nmol.L-1) and that most new compounds tested exhibited high activities of inhibition on rhAChE. The study suggests that rhAChE is available to be applied in screening AChE inhibitors in vitro.
Acetylcholinesterase ; genetics ; metabolism ; Cholinesterase Inhibitors ; analysis ; pharmacology ; HEK293 Cells ; Humans ; Indans ; analysis ; pharmacology ; Inhibitory Concentration 50 ; Kinetics ; Piperidines ; analysis ; pharmacology ; Plasmids ; Recombinant Proteins ; genetics ; metabolism ; Transfection