As a connecting bridge between innate immunity and specific immunity,the Toll-like receptors (TLRs) play a significant role in inflammation,cellular signal transduction,and cell apoptosis.TLRs are also essential factors participating in immune regulation and immune escape in the pathogenesis and development of malignant tumors.A large number of clinical and experimental research have shown that the abnormal expression of TLRs can be detected in adenocarcinoma,such as lung cancer,breast cancer and colon caner.In recent years,the relationship between gynecologic malignant tumor and TLRs has received great interest.This article will review and summarize research progress of TLRs in ovarian cancer,cervical cancer,and endometrial neoplasia.

Objective To perform mutation screening on the OTOF gene of 76 Chinese patients with sporadic auditory neuropathy for investigating whether the patients contained mutational hotspots in the OTOF gene,identifying the distribution and frequencies of OTOF mutations,and detecting new mutation points in the OTOF gene.Methods Genemic DNA samples were extracted from peripheral venous blood samples of the patients.9 primer pairs were designed using the Primer 5.0 software package for 9 exons of the OTOF gene,in which mutations had been discovered in the past.The exons were amplified using polymerase chain reaction(PCR),and direct sequencing of the PCR products was performed to detect OTOF mutations.For analysing the sequence data,the DNAStar 5.0 software package was used.Results 8 types of OTOF polymorphic alleles were discovered in this study.Among them,the 82 769delAG deletion mutation was only found in a patient diagnosed with temperature sensitive auditory neuropathy.In exon 25 of this patient's OTOF gene,the AG deletion mutation caused the replacement of amino acid at positions 993~999 and resulted in a truncated protein at position 1 000 amino acid(exon 26),which caused an early stop codon.(This protein has 1997 amino acids in all).Nevertheless,no other mutations were found in this patient's OTOF gene.The heterozygous 76 378C/T and 82 913G/A polymorphisms were single nucleotide polymorphisms(SNP) discovered in this study.Other SNP found were 56 842A/C,82 885C/A,and 92 905G/A,which had been already published by NCBI.In addition,92 995C/T and 96 888C/T were heterozygous mutations found in the exons,but did not cause the replacement of acid amino.Conclusion Eight SNPs were found in the OTOF gene of the Chinese patients in this study.However,mutations,which were previously identified in other ethnic origins in the literature,were not found in these patients.Thus,the result implied that the Chinese patients with auditory neuropathy may contain new OTOF mutations or other relevant disease-causing genes.

Aptamers are oligonucleotides or peptides those are able to bind tightly,by their specific three-dimensional shapes,to a variety of targets.Because of numerous merits ( high affinity,high specificity,small size,little immunogenicity,stable structures,and ease of synthesis),aptamers represent a valid alternative to antibodies and become a valuable research tool and show great application to fundamental research,drug selection and clinical diagnosis and therapy.The review describe the applications and the possible applications of aptamers in the diagnosis,treatment and pathogenesis of autoimmune diseases.

Objective To identify a potential correlation between cold hemoagglutinin(CHA) and diffuse panbronchiolitis(DPB) in Chinese patients.Methods Eighteen patients diagnosed as DPB from December 1996 to July 2008 in Peking Union Medical College Hospital and 60 cases of DPB reported in mainland of China from 1996 to 2008 were enrolled in the study.Results Of 18 patients diagnosed as DPB in Peking Union Medical College Hospital,only one patient showed a titer of CHA≥1:64.Of 60 cases in mainland China,48 cases were CHA positive.CHA was positive in 54.1% all cases.There may be some correlation between positive rate of CHA and medication as well as population.Conclusion Low positive rate of CHA in Chinese subjects,which is different from that of Japanese DPB patients,suggests that CHA may not be applied as a diagnostic criteria for Chinese patients.

44 obstructive sleepapnea-hypopnea syndrome ( OSAHS) patients were Supplemented by an controlla-ble appliance in their intervene treatments. The data of polysomnography ( PSG) , Epworth sleepiness scale( ESS) and quality of life-Quebec sleep questionnaire( QSQ) before and 3 treatment period months after oral appliance were recorded. Correlations between subjective and objective measure before treated with OA and subsequently investi-gate treatment mechanism of oral appliance( OA) . Correlations indexes between before and after 3 treatment with an controllable appliance, and evaluation had statistically significance ( P <0. 05 ) . Absolute correlations between PSG indexes and subjective measures before oral appliance ranged from 0. 321 ~0. 433 , and 3 treatment period months after oral appliance ranged from 0. 306~0. 437 . Both PSG indexes and quality of life improved significantly after oral appliance(P<0. 05).

BACKGROUND: "Public Health Report in 2000" shows that various indexes of body shape of the national infants increase with their age; Urban infants exceed rural even-aged ones in the body height, body mass and chest circumference; Urban infants also exceed rural even-aged ones in the mean increase of development of body shape at each index. Urban-rural disparity still exists.OBJECTIVE: To investigate the body shape of 3- to 6-year-old infants of Heibei province, and analyze the differences in body shape at different age stages between urban and rural infants.DESIGN: Cross-sectional survey.SETTING: Hebei Provincial Party School.PARTICIPANTS: Body-shape investigation was performed in 1 600 healthy 3- to 6-year-old urban and rural infants from Shijiazhuang,Cangzhou and Chengde in July 2000 with clustering stratified random sampling. Informed consents were obtained from the guardians of the infants.METHODS: By referring to related results, the body length and body mass at born of infants were investigated through questionnaire-style according to the performance criteria of "Public Health Monitoring Working Manual in 2000" formulated by general administration of sport of China.To be measured shape indexes included body height, body mass, sitting height, chest circumstance, skinfold thickness at upper buttocks, shoulder blade and abdomen, t test was used for the comparison of difference of measurement data.RESULTS: Totally 1 600 questionnaires were handed out, and all of them were completely and exactly filled in and retrieved. 800 were male and 800 were female; 800 were urban infants and 800 were rural infants. ① The body height and body mass of 3- to 6-year-old infants at born: Four year-old male infants were shorter than other age-stage infants at born. Six-year-old male infants were the longest, and the increased amplitude was the largest and the increased speed was the fastest. The body height of female infants developed in wave-tendency from born, decreased at 5 years old and rebounded at 6 years old. The body mass of male infants developed in wave-tendency, and decreased fastest in the largest amplitude at 6 years old. The body mass of female infants developed in year-by-year decreasing tendency. The increased amplitude and speed of the body mass of the female infants were the same between at 5 yearn old and at 6 years old. ② The changes in body height and body mass of 3- to 6-year-old infants: The body height and body mass of 3- to 6-year-old infants increased with age.③ Comparison of the measuring index of body shape between urban and rural 3- to 6-year-old infants: The urban male infants significantly exceeded the rural male ones in chest circumstance, skinfold thickness at upper buttocks, shoulder blade and abdomen [(55.22±3.56)vs(54.58±3.43) cm;(9.33±3.20)vs (8.34±2.29) mm;(6.19±2.55)vs (5.18±1.98) mm;(6.64 ±3.30)vs (6.05±2.49) mm, t=2.573, 5.034,2.375, 2.848,P ＜ 0.05-0.01].The body height, body mass and sitting height were close between urban and rural male infants (P ＞ 0.05). ④ Comparison of the measuring index of body shape between urban and rural 3- to 6-year-old female infants: Urban female infants significantly exceeded rural female ones in skinfold thickness at upper buttocks, shoulder blade and abdomen [(10.09±3.32) vs (9.08±2.52) mm, (6.68±2.45) vs (6.16±2.01) mm, (7.62±3.23) vs (6.84 ±3.07) mm, t=4.857, 3.266,3.481 ,P ＜ 0.01].The body height, body mass,sitting height and chest circumstance were close between urban and rural female infants (P ＞ 0.05).CONCLUSION: ①Male and female infants have the largest increased amplitude and fastest increased speed in the body height between at 3 years old and at 4 years old. Increased amplitude and increased speed develop constantly from 4 years old. ② The body mass of male infants had the largest increased amplitude at 6 years old, but that of female infants at 4 years old. The increased speed of body mass of male infants is higher at 4 years old and 6 years old than at 5 years old, and that of female infants increase with age. ③ Urban male nfants exceed rural male infants in upper body development and in vivo fat content. In vivo fat content of urban female infants is higher than that of rural ones.

BACKGROUND: Officers and intellectuals are bearing heavy government affairs and tasks of teaching and scientific research. It is practically important to acknowledge and master their body constitution, which can help to instruct them to scientifically build their bodies.OBJECTIVE: To investigate the body function and shape as well as body constitution of scientific researchers and officers in Handan, and analyze the existing problems and bring out the settlement.DESIGN: Cross-sectional study.SETTING: Hebei Provincial Committee Party School.PARTICIPANTS: A total of 499 subjects, aged 20-59 years, including238 males and 261 females were selected from government officers, university teachers and scientific researchers of institute and aeademy by using stratified and cluster random sampling. Subjects aged 20-39 were divided into adult group A (50.5%), and those with the age ranged 40-59 were taken as adult group B (49.5%).METHODS: All subjects fulfilled the questionnaire (including whether they had hospitalized history, and the smoking condition, exercise condition, reasons why did not participate in the sports, sports items that patients took part in, the place they conducted athletic activities, time of each exercise and exercise times per week etc.) and were measured of the body constitution.MAIN OUTCOME MEASURES: ①Indexes of body function and shape,including body height, body mass, chest circumference, waistline, hip circumference and skinfold at all parts of body as well as the pulse in quiet state, systolic blood pressure (SBP), diastolic blood pressure (DBP) and lung capacity etc. ②Indexes of body constitution, including grasp force,bend-ahead in sitting, stand up on one leg with eyes closed, choice reaction time simple reaction time, back strength, 10 m×4 shuttle run, and pushup (male)/sit-up (female) etc. The results of test were scaled according to the Report of Monitoring of Chinese Adult Constitution, edited by the State Physical Cultural Administration. Those with the score greater than 17 points passed, while those less than 17 points failed.③The relationship between status quo of body constitution and life style.RESULTS: A total of 499 subjects were involved in the analysis of results.①With the age increasing, the body mass in males and females were increased with circumferences being enlarged and subcutaneous fat increased, and the shape was in horizontal development. The body function in females with the age ranged 20-40 were relatively stable, and began to descend at 40. The body function in adult males descended gradually and stably with the descending speed lower than in females. The force, bursting strength of lower limbs, position sense, coordination of sight and proprioceptive sense, speed in motion and response etc. Of adult males were better than males, while the extensibility and tenacity of joint, muscle and tendon were superior in females than in males. All indexes of constitution in males aged 20-40 were better, while the tenacity and speed in motion and response of males older than 40 began to decay rapidly. ②The pass rate in males of adult group A was 87.5% and was 87.3% in females, while that in males of adult group B was 80.2% and was 74.8% in females. ③The pass rate in populations with exercise custom was greatly higher than in those without it, while the proportion of exercise people accounted little in the subjects. It accounted for 40.7% in males of adult group A and for 28.6% in females, and it accounted for 37.3% in males of adult group B, and for 31.6% in females. ④Body constitution in people who never smoke and occasionally smoke were better than in those often smoke. The pass rate in people who had been hospitalized was a little lower. ⑤The pass rate in subjects who exercised three or four times each week was much higher,and it was the highest in subjects exercised 30-60 minutes each time.CONCLUSION: ①The constitution in population older than 40 is significantly worse and worse. ②Sports activities can remarkably promote the body constitution.③Smoking has obvious side effects on body constitution.④It can reinforce the body constitution by doing sports activity for 30-60 minutes and 3 or 4 times each week.

Objective To explore the relationship between self- management and DOSE index for patients with chronic obstructive pulmonary disease (COPD). Methods The convenient sampling and Pearson correlation analysis was used in the study,a total of 81 patients was investigated with Self- Management Scale (SMS) for COPD patients and DOSE index. Results The relationship of symptom management and DOSE index for COPD patients was a positive correlation (r=0.259, P<0.05);with self- efficacy was a negative correlation(r=-0.288, P<0.05).But self- management, daily life management, emotion management and information management with DOSE index were no correlation (P>0.05). Conclusions Through health education, nurses should strengthen to effectively help patients manage symptoms, enhance self- efficacy, improve the patients′quality of life.

Objective To analyze types, mechanism and forensic identification for traumatic visual loss. Methods 109 cases of the evaluation for impairment and disability on traumatic visual loss were retrospectively analyzed, including interocular differences, anisometropia, left or right eye and the distributions of visual acuity, etc. Results The male-to-female ratio was 5.8:1, mainly 30~59 years of age. The ratio of monocular versus binocular injuries was 17.17:1. The ratio of left versus right injured eye was 1.39:1 in intentional injury cases. 115 eyes were injured, 83 of which were in low vision or blindness. 95 patients(87.16%) had large interocular differences( ≥ 3 lines). There were 9 patients with anisometropia from 2.25D to 6.00D and 23 patients with anisometropia larger than 6.00D. Conclusion Interocular differences and anisometropia are common in practice, which are not included in the expertise standard yet. Functional vision should be considered when developing the expertise standard.

OBJECTIVE:To establish the method for the content determination of total saponins in Naomaitong.METHODS:Colormetric method was applied using panoxadiol as control.The detection wavelength was set at 556 nm.RESULTS:The linear range of panoxadiol was 2.03～10.15 ?g?mL-1(r=0.999 6) with an average recovery of 100.69%(RSD=1.76%,n=6).CONCLUSION:The method is easy to operate with high precision,accuracy and good reproducibility for the quality control of Naomaitong.