No abstract available.
Female ; L-Lactate Dehydrogenase* ; Lactic Acid* ; Ovary*

No abstract available.
Hematologic Neoplasms* ; L-Lactate Dehydrogenase* ; Lactic Acid*

OBJECTIVE: Creatine phosphokinase-MM(CPK-MM) and lactate dehydrogenase(LDH) are well known indices of muscular injury. To know the degree of muscular injury during spinal surgery, the author report serial measurement of serum CPK-MM and LDH4 level. METHODS: The authors investigated 23 patients who underwent lumbar spinal surgery without bone fusion or instrumentation. Peripheral venous blood samples were serially collected 1 day before surgery, operation day, postoperative 3rd, 7th, 14th and 28th day. Postoperative back pain was measured by visual analogue scale. We evaluated the relationship between number of operation level, serial changes of serum CPK-MM, LDH4, duration of surgery and postoperative back pain. RESULTS: CPK-MM activity was higher after surgery than before it and reached at maximal level on the postoperative 3rd day, and it was returned to normal level on the postoperative 7th day. The score of postoperative back pain scale was the highest on the postoperative 3rd day. CPK-MM activity was significantly correlated with operation level, duration of surgery, and postoperative back pain(P<0.05). In contrast, serum level of LDH4 showed no significant correlation with duration of surgery(P>0.05). CONCLUSION: Postoperative muscle injury is inevitable in all patient who underwent spinal surgery, and these injuries are related extent of exposure and duration of surgery. To reduce muscle injury and postoperative back pain, less invasive and shorter surgery in time is recommend.
Back Pain ; Creatine ; Humans ; L-Lactate Dehydrogenase ; Lactic Acid ; Spine*

BACKGROUNDStreptococcus (S.) oligofermentans is a newly identified bacteria with a yet to be defined mechanism of sucrose metabolism that results in acid production. This study aimed to investigate the biochemical mechanisms of S. oligoferm-entans glucose metaolism.

METHODSThe S. oligofermentans LMG21532, Lactobacillus (L.) fermentum 38 and the S. mutans UA140 were used to characterize sucrose metabolism by measuring lactate dehydrogenase (LDH) activity and lactic acid production. Continuous dynamics and high performance capillary electrophoresis were used to determine LDH activity and lactic acid production, respectively, from bacteria collected at 0, 10 and 30 minutes after cultured in 10% sucrose.

RESULTSThese analyses demonstrated that LDH activity of the three bacterial strains examined remained stable but significantly different throughout the sucrose fermentation process. The S. oligofermentans LDH activity ((0.61 ± 0.05) U/mg) was significantly lower than that of L. fermentum ((52.91 ± 8.97) U/mg). In addition, the S. oligofermentans total lactate production ((0.048 ± 0.021) mmol/L) was also significantly lower than that of L. fermentum ((0.958 ± 0.201) mmol/L). Although the S. oligofermentans LDH production was almost double of that produced by S. mutans ((0.32 ± 0.07) U/mg), lactic acid production was approximately one sixth that of S. mutans ((0.296 ± 0.058) mmol/L). Additional tests examining pyruvic acid production (the LDH substrate) demonstrated that lactic acid concentrations correlated with pyruvic acid production. That is, pyruvic acid production by S. oligofermentans was undetectable following sucrose incubation, however, (0.074 ± 0.024) and (0.175 ± 0.098) mmol/L pyruvic acid were produced by S. mutans and L. fermentum, respectively.

CONCLUSIONS. oligofermentans is incapable of fermenting carbohydrates to produce enough pyruvic acid, which results in reduced lactic acid production.

L-Lactate Dehydrogenase ; metabolism ; Lactic Acid ; metabolism ; Pyruvic Acid ; metabolism ; Streptococcus ; metabolism ; Sucrose ; metabolism

Urinary lactate dehydrogenase (U-LD) isozyme assays were performed by cellulose acetate electrophoresis on the patients with clinically proven upper urinary tract infection (U-UTI) (N=21), lower urinary tract infection (L-UTI) (N=l5) and undetermined site of urinary tract infection (N =l4) as well as normal controls(N=l5). The results of this study are summarized as follows: * Total U-LD in normal controls (73.8 +/- 37.4 U/1) was significantly lower than in patients with upper UTI (145.9 +/- 86.8 U/I(p<0.01) or lower UTI (126.3 +/- 88.4 U/I) (p<0.05). * In normal controls and patients with L-UTI, LDI and LD2 predominated ('fast zone' pattern), and in patients with U-UTI, mainly LD5 and LD4 predominated('slow zone' pattern).
Diagnosis, Differential* ; Electrophoresis, Cellulose Acetate ; Humans ; L-Lactate Dehydrogenase* ; Lactic Acid* ; Urinary Tract Infections* ; Urinary Tract*

Influence of acute and chronic carbon monoxide poisoning on the rat prostate has been studied enzymologically and the following results were obtained: 1. An increasing tendency of the total lactate dehydrogenase activity in the prostate tissue was observed is the chronic poisoning groups. 2. The lactate dehydrogenase isoenzyme distribution pattern was not remarkably influenced by chronic poisoning. 3. The lactate dehydrogenase isoenzyme distribution pattern is the prostate tissue was as LDH5> LDH4> LDH3> LDH2> LDH1.
Animals ; Carbon Monoxide Poisoning* ; Carbon Monoxide* ; Carbon* ; L-Lactate Dehydrogenase* ; Lactic Acid* ; Poisoning ; Prostate* ; Rats*

Influence of acute and chronic carbon monoxide poisoning on the rat testis has been studied enzymologically and the following results were obtained: Experimental animals were divided as 1) control, C1 (6 animals) 2) acute poisoning with 0.5% carbon monoxide until death, C2 (10 animals) 3) chronic poisoning with 0.05% carbon monoxide for 2 hours daily E1 (5 animals)...3 days/week for 8 weeks E2 (6 animals)...3 days/week for 4 weeks 4) acute poisoning until death with 0.5% carbon monoxide after chronic poisoning with 0.05% carbon monoxide E3 (5 animals)...chronic poisoning for 8 weeks plus acute poisoning E4 (5 animals)...chronic poisoning for 4 weeks plus acute poisoningUsing Neiland's method, the total activities and distribution patterns of lactic dehydrogenase in rat testis were measured. All of the data were analyzed statistically. 1. The total activities of lactic acid dehydrogenase in all experimental groups seemed to increase. In the group E3, the increase was statistically significant.(p<0.05) 2. No significant changes were observed in the lactic acid dehydrogenase isoenzyme distribution pattern of the testis. both is the control and experimental groups. 3. The lactic acid dehydrogenase distribution patterns were as fraction l>2>4>3>5 4. No significant histopathological changes were observed in the rat testis.
Animals ; Carbon Monoxide Poisoning* ; Carbon Monoxide* ; Carbon* ; L-Lactate Dehydrogenase* ; Lactic Acid* ; Oxidoreductases ; Poisoning ; Rats* ; Testis*

The purpose of this study was to compare body composition, anaerobic capacity, and hematologic parameters after intermittent complex training in high school soccer players. The subjects were 15 high school soccer players (age, 15.60±0.82 years; height, 171.93±5.65 cm; body weight, 61.32±7.43 kg). Clinical variables included body composition, anaerobic capacity, and hematologic parameters. Data were analyzed to compare between trials by paired sample t-test. As a result, there were significant differences in body weight (p<0.05) and muscle mass (p<0.05) of body composition after 4 weeks. There were significant differences in shuttle run (p<0.001) and heart rate in rest (p<0.05) of aerobic performance after 4 weeks. There were significant differences in lactate dehydrogenase (p<0.001), creatine kinase (p<0.001), all out lactic acid (p<0.01), and red blood cell (p<0.001) of hematologic parameters after 4 weeks. In summary, intermittent complex training for 4 weeks showed strong influences on body composition and hematologic parameters variables in high school soccer players.
Body Composition* ; Body Weight ; Creatine Kinase ; Erythrocytes ; Heart Rate ; Humans ; L-Lactate Dehydrogenase ; Lactic Acid ; Soccer*

BACKGROUND: As a tumor marker, lactate dehydrogenase is widely used as a prognostic factor in malignant lymphomas and a therapeutic monitor in ovarian dysgerminoma. But in other tumors, the clinical role of lactate dehydrogenase is not clear yet. To investigate the role of LD as a tumor marker, we analyzed patients with various cancers and high LD level. METHODS: The serum LD level was assayed in the cancer patients who visited our hospital. For the patients with high LD level, isoenzyme was also assayed. The response of chemotherapy was classified according to the WHO criteria into remission, which covers complete and partial remission, stable disease and progressive disease. The relationship between the tumor response and the LD response was examined in terms of sensitivity, specificity and positive predictive value. RESULTS: A total of 100 cancer patients were found to have high LD level; among them, 76 patients underwent chemotherapy. There were 38 patients with LD increased and 38 patients with LD decreased after chemotherapy. No patient was in remission, 6 patients in stable disease, and 32 patients in progressive disease among 38 patients with increased LD level; 18 patients in remission, 10 patients in stable disease, and 10 patients in progressive disease among 38 patients with decreased LD level. The sensitivity of LD increase in the patients with tumor progression is 76.2%, the specificity 73.7%, and the positive predictive value 84.2%(71.4%, 25.0% and 76.9% respectively in stomach cancer patients). The sensitivity of LD decrease in the responsive patients is 100%, the specificity 65.5% and the positive predictive value 47.4%.(100%, 64.7% and 14.3% in stomach cancer patients respectively). The LD isoenzyme pattern was not specific in this study. CONCLUSION: Serum LD measurement may be helpful in monitoring tumor response, especially for stomach cancer, after chemotherapy.
Drug Therapy ; Dysgerminoma ; Humans ; L-Lactate Dehydrogenase ; Lactic Acid* ; Lymphoma ; Sensitivity and Specificity ; Stomach Neoplasms

Serum lactate degydrogenase (LD) and LD isoenzyme activities were evaluated in sera from 74 patients of coal workers' pneumoconiosis (CWP) to investigate biochemical changes of these enzyme activities according to radiographical profusions of CWP. Subjects were participated patients in the detailed examination of pneumoconiosis in December 1989. LD activities were evaluated by lactate to pyruvate methods and isoenzyme activities were determined by agarose gel electrophoresis. And small opacity profusions on chest radiographs of subjects were evaluated by radiologist. The results were as follows: 1. LD activities were not significantly different among categories of radiographical profusions. 2. LD4 activities were shown difference significantly among categories of radiographical profusions (category 1: 8.2+/-2.69 U/l, category 2: 10.1+/-3.90, category 3: 10.6+/-2.63; p<0.05). 3. Activities of LD(1), LD(2), LD(3), and LD(5) were not different significantly among categories of radiographical profusions. Conclusionly, serum LD(4) activity seems to be increased by fibroplasia of CWP, but increasing activities of LD4 should not affect serum LD activities significantly.
Anthracosis* ; Coal* ; Electrophoresis, Agar Gel ; Humans ; Isoenzymes* ; L-Lactate Dehydrogenase* ; Lactic Acid* ; Pneumoconiosis ; Pyruvic Acid ; Radiography, Thoracic* ; Thorax*