Objective To study changes of aspartate(ASP) and glutamaic acid(GLU) in cerebral cortex of neonatal Sprague-Dawely(SD) rats after hypoxia-ischemia and nitric oxide synthase(NOS) immunoactive expression in cerebral neurons were examined to explore mailuoning′s protective effect on hypoxia-ischemia brain damage(HIBD).Methods The HIBD model was established as follows.The right common carotids of the neonatal SD rats 7 days were temporaily ligatured for 1 hour.Then the neonatal SD rats were exposed to 8% oxygen and 92% nitrogen gas mixture for 2 hours. The ASP and GLU were determined in right cerebral cortex using chromatograph,compared with sham-operated group and mailuoning administrated. Ultrastructure changes of neurons in the right cerebral cortex of neonatal SD rats were observed after sham-operated,hypoxia-ischemia and mailuoning administrated using electronmicroscope.Results The level of excitatory amino acid was promoted in right cerebral cortex after hypoxia-ischemia.The volume of excitatory amino acid was reduced sharply mailuoning administrated. Ultrastructure of neurons in the cerebral cortex showed serious injure after hypoxia-ischemia and ultrastructure of neurons in the cerebral cortex appeared slight damage.Conclusion Mailuoning may possess protective effects to the neurons after hypoxia-ischemia through supplying blood to neurons reducing release of excitatory amino acid.

OBJECTIVETo evaluate of the efficacy and safety of entecavir (ETV) combined with Shenxian Yiganling (SY) versus ETV therapy on patients with HBeAg-positive chronic hepatitis B (CHB) for 48 weeks.

METHODSOne hundred and sixty-four CHB patients were assigned to two groups with the cohort study: the ETV combined with SY treatment group and the ETV control group. The alanine aminotransferase (ALT), the undetectable HBV DNA level, and HBeAg negative conversion rate, and HBeAg serological negative conversion rate were observed before and after treatment.

RESULTSAt week 48, there was no significant difference in the normalization of ALT levels (70.00% vs 67.61%, P > 0.05) and undetectable HBV DNA levels (72.50% vs 73.24%, P > 0.05) between the two groups. There was significant difference in the HBeAg negative conversion rate (39.44% vs 23.75%) and HBeAg serological negative conversion rate (32.39% vs 15.00%) (both P < 0.05).

CONCLUSIONETV combined with SY promoted the HBeAg serological negative conversion rate possibly through the recovery of the immune functions.

Adult ; Antiviral Agents ; therapeutic use ; DNA, Viral ; blood ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Guanine ; analogs & derivatives ; therapeutic use ; Hepatitis B e Antigens ; blood ; Hepatitis B, Chronic ; blood ; drug therapy ; Humans ; Male ; Middle Aged ; Phytotherapy ; Prospective Studies ; Treatment Outcome ; Young Adult

Objective To investigate the effects of extracellular ATP on the expression of(Mi- crotubule associated proteins MAP-2)and the recovery of motor function after spinal cord injury in rats. Methods Six rats were selected randomly from 66 adult healthy Wistar rats as the normal control group,the rest animals were divided into two groups after contusion injury was performed by drop weight method with Allen impactor:Group A(ATP group)and Group B(control group),each group contained thirty rats.At days 1,3,7,14,and 28 after injury,the rats were killed,the expression of MAP-2 was detected with immunohistochemistry.The expression of MAP-2 in the adjacent area was quantitatively an- alyzed with a computer image analysis system.The recovery of motor function after spinal cord injury was assessed with improved Tarlov scores.Results The expression of MAP-2 was higher in Group A than in Group B after spinal cord injury in rats.Significant difference was revealed by the expression of MAP- 2 between the two groups at days 14 and 28 after injury(P

OBJECTIVETo explore the relative factors on the failure in digit replantation in order to take preventions to control the risk factors.

METHODSFrom January 2013 to December 2013, 236 consecutive patients (311 fingers) underwent digit replantation were collected to analyze retrospectively, involving 183 males and 53 females with an average age of 34.5 years old ranging from 2 to 62 years old (6 cases under 6 years old and 230 cases elder than 6 years old). There were 51 thumbs, 87 index fingers, 78 middle fingers, 63 ring fings and 32 little thumbs. Forty cases(forty fings) who were failured as the observation group, the others as the control group. The factors of age, gender, finger, cause of injury, smoking history, ischemia duration, plane of division, condition of venous drainage and condition of arterial repair we assessed.

RESULTSAll 236 cases with 311 fingers were replanted, 40 fingers were failured after operation. The relative factors on the failure in digit replantation included smoking history, cause of injury, plane of division, condition of venous drainage and condition of arterial repair (P< 0.05). There were no significant correlation between the failure and age, gender, finger and ischemia duration (P>0.05).

CONCLUSIONSmoking history, causes of injury, plane of division, condition of venous drainage and condition of arterial repair are risks of failure in digit replantation. Before choosing the type of operation, it should be think about the patient's general conditions, injury status, grasp firmly the operative indications and actively carry out surgical treatment.

Adolescent ; Adult ; Child ; Female ; Finger Injuries ; surgery ; Fingers ; surgery ; Humans ; Male ; Middle Aged ; Reconstructive Surgical Procedures ; Replantation ; Retrospective Studies ; Risk Factors ; Thumb ; injuries ; surgery ; Treatment Failure ; Young Adult

Objective To investigate the clinical efficacy of staging treatment with various acupuncture therapies mainly on Yifeng (TE17) point for the treatment of peripheral facial paralysis. Methods One hundred and two peripheral facial paralysis patients were randomly divided into trial group and control group, 51 patients in each group. The trial group was given acupuncture mainly on Yifeng point, by Yang needling(plum-blossom needling) for acute stage, Qi needling(concentrated triple-needling) for resting stage and Bang needling(lateral needling) for recovery stage. The control group was given conventional acupuncture. The treatment for the two groups was performed once a day, 5 continuous days constituted a course, and the treatment lasted 3 courses. Before treatment and after treatment for 1, 2, 3 course (s), facial nerve function scores were evaluated with House-Brackmann Grading Scale (HB) and the scores of pain in head and face were evaluated with Visual Analogue Scale (VAS). And the clinical efficacy of the two groups was assessed after treatment. Results (1) After treatment for 3 courses, the cure rate and total effective rate of the trial group were 82.35％, 100.00％, and those of the control group were 60.78％, 86.27％, respectively. The differences between the two groups were significant (P <0.05). (2) After treatment, HB scores and VSA scores of patients with facial pain and headache in the two groups were improved after treatment (P < 0.05 compared with those before treatment), and the improvement in the trial group was superior to that in the control group (P < 0.05). Conclusion Staging treatment with various acupuncture therapies mainly on Yifeng point is more effective for the treatment of peripheral facial paralysis than conventional acupuncture.

Plasma obestatin level was determined in patients with impaired glucose regulation and type 2 diabetes mellitus.The plasma obestatin levels in patients of both groups were significantly decreased as compared with that in controls.Plasma obestatin level was negatively correlated with body mass index,HbA_(1C),waist-to-hip ratio,plasma insulin and HOMA-IR.Obestatin level seems to be related with metabolic disorder.

Adult ; Aged ; Female ; Fibroblast Growth Factor 1 ; genetics ; Fibroblast Growth Factor 2 ; genetics ; Humans ; Middle Aged ; Neoplasm Staging ; Neoplasms, Glandular and Epithelial ; metabolism ; pathology ; Ovarian Neoplasms ; metabolism ; pathology ; RNA, Messenger ; analysis ; Receptor Protein-Tyrosine Kinases ; genetics ; Receptor, Fibroblast Growth Factor, Type 1 ; Receptors, Fibroblast Growth Factor ; genetics

Enterovirus A, Human ; Enterovirus Infections ; drug therapy ; Female ; Humans ; Infant ; Meningitis, Viral ; drug therapy

Objective To observe the clinical efficacy of stage-based treatment of peripheral facial paralysis with Yifeng (TE 17) selected as the major point treated by different acupuncture methods.Method A total of 102 patients with peripheral facial paralysis were randomized into a treatment group and a control group, 51 cases each. The treatment group was intervened by selecting Yifeng (TE 17) as the major point, treated with centro-square needling in the acute stage, triple needling in the resting stage, and proximal needling in the remission stage; the control group was intervened by ordinary acupuncture. The two groups were treated once a day, 5 d as a course of treatment. Before and after 3 treatment courses, the House Brackmann (HB) facial nerve grading scale and Visual Analogue Scale (VAS) in those patients complicated with pain were observed, and the clinical efficacies were compared.Result The total effective rate and recovery rate were respectively 100.0％ and 82.3％ in the treatment group versus 86.3％ and 60.8％ in the control group, and the between-group differences were statistically significant (P<0.05). The HB scores were significantly changed respectively after 1, 2 and 3 treatment courses in the two groups compared with the pre-treatment scores (P<0.05). The HB scores in the treatment group were significantly different from those in the control group respectively after 1, 2 and 3 treatment courses (P<0.05). The patients complicated with facial pain or headache presented significant differences in VAS score respectively after 1, 2 and 3 treatment courses in the two groups (P<0.05). In comparing the VAS score in the patients complicated with facial pain or headache, there were significant differences between the two groups respectively after 1, 2 and 3 treatment courses (P<0.05).Conclusion Stage- based treatment with Yifeng (TE 17) selected as the major point treated with different acupuncture methods can produce valid efficacy in treating peripheral facial paralysis.

OBJECTIVE To explore the protective effects and mechanisms of Ginsenoside Rg1 (Rg1) on H2O2-induced hippocampal neurons aging in vitro. METHODS The primary culture hippo-campal neurons(7 d)were randomly placed into six groups:normal control group,H2O2(200 μM)treat-ment group,and H2O2+Rg1(1,5 and 10μM)groups.The neurons were with Rg1(1,5 and 10 μmol·L-1) for 6h. H2O2(200 μmol·L-1) was added to the medium and incubate for 18 h. The Dihydroethidium (DHE) staining was performed for ROS production assessment. The LDH release and Hoechst 33258 were performed to examine the neuronal damage and apoptosis. The immunoblot was used to deter-mine the expression of β-Gal,NOX2,p22phox,p47phox,NLRP-1,ASC and Caspase-1 in hippocampal neurons.The ELISA was performed to detect the levels of IL-1β and IL-18 released in the supernatant in hippocampal neurons.RESULTS Rg1(5 and 10 μmol·L-1)significantly reduced the ROS production, attenuated H2O2-induced neuronal damage and apoptosis (P<0.05, P<0.01). The immunoblot results showed that Rg1(5 and 10 μmol·L-1)treatment significantly decreased the expression of β-Gal,NOX2, p22phox,p47phox,NLRP-1,ASC and Caspase-1 in hippocampal neurons(P<0.05,P<0.01).Additionally, Rg1(5 and 10 μmol·L-1)treatment significantly decreased IL-1β and IL-18 release in the supernatant. CONCLUSION The protective effect of Rg1 in H2O2-induced hippocampal neurons aging may be due to inhibit NOX2-NLRP1 activation.