Possessing the unique feathers of high targeting and inducing the active immunity of patients,magnetic-mediated hyperthermia(MMH)has been regarded as a very promising cancer-treatment approach.Researches about in-stent MMH for esophagus cancel have been widely carried out with focusing on heating mechanism,feasibility studies and clinical trials.

Objective To compare the clinical curative effect between transjugular intrahepatic portosystemic stent-shunt (TIPS) and TIPS together with gastric coronary vein embolization (GCVE) in treating cirrhosis portal hypertension (PHT) associated with upper gastrointestinal hemorrhage (UGH),and to discuss the necessity,feasibility and clinical curative effect of TIPS plus GCVE.Methods The clinical data of 38 PHT patients with UGH,who were admitted to authors' hospital during the period from April 2010 to May 2012,were retrospectively analyzed.Only TIPS was employed in 15 patients (group A),and TIPS plus GCVE was adopted in 23 patients (group B).Before and after operation,the indexes,hemodynamics of portal vein and spleen,the morphology of spleen,and the degree of gastrointestinal varices were determined and analyzed.The patients were followed up to observe the occurrence of postoperative complications.Results In both groups,the postoperative portal vein pressure showed an obvious reduction with accelerated velocity of flow,and the splenic venous congestion index was decreased,these changes were statistically significant when compared with the preoperative ones (P＜0.05),and which was more obvious in group B than in group A (P＜0.05).After the treatment,the esophagogastric varices (EGV) was obviously improved,the improvement rates of group B and group A were 94.7％ and 66.6％ respectively,the emergency hemostasis rates of group B and group A were 100％ and 75.0％ respectively,The rates of re-bleeding were 4.3％ and 28.5％ respectively;the above results of group B were statistically better than those of group A (P＜0.05).No statistically significant differences in liver function indexes existed between preoperative values and postoperative ones in the same group as well as in group comparison (P＞0.05).The differences in the postoperative hepatic encephalopathy (HE) occurrence and in primary patency rate of stent between the two groups were not statistically significant (P＞0.05).In both groups,the re-intervention patency rate was 100％.The incidence rate of HE in patients in whom the distal end of stent was located in the left branch of portal vein was strikingly lower than that in patients in whom the distal end of stent was located in the right branch of portal vein (P＜0.05).Conclusion For the treatment of PHT associated with UGH,TIPS combined with GCVE carries reliable curative effect,this therapy is superior to simple use of TIPS.

OBJECTIVE:To discuss the importance of essential drug supply system for national drug policies and the role of public governance theory in the supply of essential drugs.METHODS:In view of the status of the construction of medical insurance system in China for residents in city and countryside,some strategies for the improvement of the health service system were put forward from aspect of public governance.RESULTS:Taking service as orientation,changing the government administration to service administration,introducing non-profit organization and bringing the function of non-public ownership into full play can help improve the national essential drug policy and drug supply ensuring system and guarantee the people's accessibility to essential drugs and medication safety.CONCLUSION:It is urgent to establish the supply and marke-ting system of essential drugs.

Objective To establish the mouse model of sclerotic skin. Methods The sclerotic skin was induced by local injections of bleomycin (BLM) in C3H and BALB/c mice. The injection solution was prepared with BLM at the concentration of 200?g/mL in PBS. In the test group 0.1 mL BLM solution was injected daily into the back of the mice for 3 weeks. For the control mice same amount of PBS was injected daily for 3 weeks. After 3 weeks, the histology of the skin and lungs was compared between the different groups, also the changes of skin thickness and quantity of collagen. Results After the treatment of BLM sclerotic skin was observed in both C3H and BALB/c mice, in which the thickness of skin and the quantity of collagen (6 mm ? 6 mm) were higher than those in the control mice (P

Objective To investigate the predictive value of serum histone deacetylase 3(HDAC3) ,cystatin C(CysC) and albu‐min levels on a large area of myocardial infarction .Methods According to whether heart failure and (or) cardiogenic shock occur‐ring during hospitalization ,102 patients with acute myocardial infarction(AMI) were divided into the two groups :the non - compli‐cating heart failure and (or) cardiogenic shock group(n= 63) and the complicating heart failure and (or) cardiogenic shock group(n= 39) .Then according to whether the creatine kinase(CK‐MB) peak value was greater than 200 IU /L ,102 AMI patients were di‐vided into two groups :CK‐MB peak values ≥ 200 IU /L group(n= 58) and the CK‐MB peak values < 200 IU / L group(n= 44) .The serum HDAC3 ,CysC and albumin levels were detected at early morning on 2 d of admission in all patients .Results Compared with the non - complicating heart failure and (or) cardiogenic shock group ,serum HDAC3 and CysC levels in the the complicating heart failure and (or) cardiogenic shock group were significantly increased(P< 0 .01) ,while serum albumin protein level was significantly in the occurrence of heart failure and(or)cardiogenic shock group .Compared with the CK‐MB peak value < 200 IU /L group ,serum HDAC3 and CysC levels in the CK‐MB peak value > 200 IU /L group were significantly increased (P< 0 .01) ,while serum albumin level was significantly decreased(P< 0 .01) .Conclusion Serum HDAC3 ,CysC and albumin levels have certain predictive value on a large area of myocardial infarction and conduce to judge the prognosis of patients .

Minimal hepatic encephalopathy (MHE)is defined as early hepatic encephalopathy without grossly evident neurologic abnormali-ties,but with cognitive deficits that can be revealed by psychometric testing.However,numerous studies have shown a lack of obvious clini-cal manifestations in MHE patients,and there is no international unified diagnostic gold standard for this disease.Affected patients′quality of life and ability to work are markedly impaired,and some even gradually progress to symptomatic hepatic encephalopathy.The treatment methods for MHE mainly include eliminating the precipitating factors for this disease and medication.This review presents the options for treatment of MHE and gives an objective evaluation of the efficacy of each treatment scheme.Currently,several treatment methods are used together,but no studies have demonstrated that combined use of treatment methods can improve the treatment outcome.

OBJECTIVE:To optimize the formulation of indomethacin-loading solid lipid nanoparticle(SLN). METHODS:Us-ing indomethacin as model drug,glyceryl behenate as oil phase,poloxamer 188 and polyethylene glycol-12-hydroxystearic acid as emulsifier,with turbidity,entrapment efficiency and drug loading amount as index,Box-Behnken response surface methodology was used to optimize the amount of oil phase,emulsifier-oil phase ratio,drug-oil phase ratio. The physicochemical properties of SLNs were characterized by SEM and DSC. RESULTS:The optimal formulation was as follows as oil phase of 0.91%,emulsifier-oil ratio of 1∶1,drug-oil phase ratio of 1∶5. The turbidity,entrapment efficiency and drug loading amount of prepared nanoparticle were 1 025-1 030 NTU,98.94%-99.08%,2.43%-2.46%,respectively;particle size and polydispersity index(PDI)were 181.5-182.3 nm and 0.340-0.341(n=3). The results of DSC showed that indomethacin was not present in crystalline state dispersed into SLNs. CONCLUSIONS:The optimal formulation is screened successfully,and indomethacin-loaded SLNs have been prepared.

BACKGROUND: In the early stage following spinal cord injury, a series of changes in metabolism and secretion take place, such as accelerated bone resorption and decreased bone formation. Determination of the biochemical markers of bone metabolism early after spinal cord injury may help understand the metabolic changes and facilitate risk evaluation for osteoporosis on such occasion.OBJECTIVE: To study the earlychanges of bone mass and bone metabolism indices in guinea pigs with spinal cord injury and evaluate the impact of spinal cord injury on bone metabolism.DESIGN: Randomly controlled animal experiment. SETTING: Department of Rehabilitation Medicine, Zhongshan Hospital of Fudan UniversityMATERIALS: The experiment was conducted in the Institute of Rehabilitation Medicine, Zhongshan Hospital of Fudan University in March 2004. Twenty-three male guinea pigs with body weight of 427-710 g (averaging 570 g) were provided by the Experimental Animal Center,Zhongshan Hospital of Fudan University and divided into experimental group (n=10) and normal control group (n=13). All the guinea pigs were kept under the same environment and fed with standard food for two weeks.METHODS: Small microsurgery scissors was used to induce a 1.5-mm transverse incision of the spinal cord, with the bleeding carefully managed and the wound closed. Thirty minutes after surgery, the guinea pigs recovered consciousness with bilateral lower limb paralysis (hypertonic paraplegia) and loss of superficial and deep sensations, but without urinary and fecal incontinence. The animals in the control group had sham operation. After housing in the same environment with standard food for 2 weeks, urine,serum and shinbone samples were collected from the animals for measurement of serum bone alkaline phosphatase (BALP), urinary pyridinoline (Pyd)/creatinine (Cr) and Ca/Cr. t test and H test were used for comparison of the samples between the two groups.parison of biochemical markers for bone formation between the two groups.RESULTS: Ten animals were analyzed in each group. No significant difference was observed in the BMD and bone dry weight between the two groups (P ＞ 0.05), but the bone ash weight of experimental group was remarkably lower than that of the control group [(0.284±0.050) vs (0.361±0.064) mg, t=3.254, P ＜ 0.05). The experimental group had significantly lower serum BALP than the control group [(0.640±0.240)vs (1.328±0.606) U/L, H=9.499, P ＜ 0.05), but higher urinary Pyd/Cr [(0.206±0.080) vs (0.117±0.037), P ＜ 0.01) and Ca/Cr [(0.003±0.003)vs (0.002±0.002), P ＜ 0.05] than the control group (H=9.676, 4.124,P ＜ 0.05).CONCLUSION: After the spinal cord injury, bone resorption remarkably increases and the function of the osteoblasts is inhibited. Bone metabolic abnormalities can occur in the early stage of spinal cord injury, and the biochemical indices for bone metabolism are sensitive in evaluating the risks for osteoporosis after spinal cord injury.

BACKGROUND: The invasion of highly metastatic carcinoma cells on reconstituted basement membrane component is considered as a common model for in vitro experimental studies of invasion and metastasis of malignant tumors. It has been reported that synthetic peptide influences some tumor cells. However, the effect of synthetic peptide on highly metastatic human lung giant-cell carcinoma cell(PG) has been rarely studied.OBJECTIVE: To study the effects of synthetic peptide RGD, YIGSR and their derivatives on invasive ability of PG in vitro.DESIGN: A completely randomized controlled trial was conducted.SETTING and MATERIALS: The human lung highly metastatic tumor and NIH3T3 cell line were both supplied by the Department of Pathology of Beijing Medical University. The synthetic peptides were provided by the Synthetic Laboratory of the Institute of Materia Medica of the Chinese Academy of Medical Sciences and Chengde Medical College. Transwell invasion chamber was purchased from Costor Company. The whole experiment was conducted at the Department of Biochemistry of Chengde Medical College.INTERVENTIONS: NIH3T3 cell line was used to prepare chemotactic factor. PG cells were seeded in Transwell invasion chamber. Under the effect of chemotactic factor, the effects of synthetic peptides on PG invasive ability were examined.MAIN OUTCOME MEASURES: PG cell number of passing through the reconstituted basement membrane was used as an index to evaluate the invasive ability of tumor cells.RESULTS: An appropriate number of PG cells were cultured for 10 hours with chemotactic factor, and the number ofinvasive cells reached a number range that can be applied to statistical analysis(50 to 100 invasive cells). After 100 mg/L of synthetic peptides RGD, YIGSR were incubated for 48 hours with PG cells,the rate of inhibition on invasive ability was 53.63% and 36.86% respectively, and 0% and 6.48% respectively after incubation for 10 hours.CONCLUSION: The PG cells were the proper cells for in vitro experimental studies of invasive ability of cancer cells for the purpose of screening of anti-tumor medicine. RGD and Juncha/Zacha-peptide YIGSR showed a strong activity in inhibiting tumor invasion and a potential value in prevention and treatment of malignant tumor development.

BACKGROUND:Basic fibroblast growth factor is a pluripotent cytokine that can promote the proliferation of mesodermal and neuroectodermal cells.
OBJECTIVE:To observe the effect of basic fibroblast growth factor in human periodontal ligament cells cultured in vitro.
METHODS:Human periodontal ligament cells at passage 5 were inoculated into the 96-wel plates at the density of 1×108/L, and were randomly divided into four groups. The cells were cultured inα-MEM containing 15%fetal bovine serum and 0, 1, 10, 100μg/L basic fibroblast growth factor, respectively. At 1, 3, 5, 7 days of the culture, the cellproliferation was determined, and the activity of alkaline phosphates was detected at 1 and 7 days.
RESULTS AND CONCLUSION:There were significant differences in the proliferation of human periodontal ligament cells among the four groups (F=6.586, P=0.024). As the increase of the basic fibroblast growth factor concentrations, the absorbance value was gradual y increased and reached the peak in 100μg/L basic fibroblast growth factor group (P<0.05). The alkaline phosphatase activity in basic fibroblast growth factor groups was lower than that of the control group (P=0.000), the higher the concentration was, the lower activity was (P<0.05). Results show that basic fibroblast growth factor can promote the proliferation of human periodontal ligament cells and inhibit the activity of alkaline phosphatase, and the effect is concentration-dependent.