Administration, Oral ; Humans ; Rotavirus Infections ; immunology ; prevention & control ; Viral Vaccines ; administration & dosage ; immunology

Orthodontic Anchorage Procedures

Objective To evaluate the value of mediastinoscopy in the diagnosis of undifferentiated mediastinal diseases. Methods A total of 35 mediastinoscopies were performed for patients with undifferentiated mediastinal disease from November 1999 to February 2002 and suspicious tissues at peritracheal,retrosternal,subcarinal,or bilateral hilar areas were obtaiued for pathological examination. Results Thirty four of 35 patients with undetermined mediastinal diseases had definite pathologic diagnosis.The diagnosis accuracy was 97%(34/35).Of the 34 patients,18 were diagnosed as malignant accounting for 52.9%.Sixteen of the 34 were diagnosed as benign,accounting for 47.1%.The diagnostic consistency of preoperation and postoperation was 47.1%(16/34).The misdiagnosis of preoperation was 52.9%(18/34).In this series,the benigns were apt to lead to misdiagnosis.10 of 16 patients with benign disease were diagnoscd as initially malignancies,representing 62.5%.In the entire group one patient had complications (2.9%).Yet no perioperative death occurred. Conclusions Mediastinoscopy is a valuable procedure which helps mede a correct diagnosis of mediastinal diseases.Given its safety and usefulness It is suggested that mediastinoscopy should be used routinely in the diagnosis of undifferentiated mediastinal diseases.

1.0 cm) by radiographic examinations, video-mediastinoscopy showed positive results in 30 cases, and negative in 14 cases, in which the surgical exploration found no evidence of metastasis to mediastinal lymph nodes. No postoperative complications and mortality were observed. Conclusions Video-mediastinoscopy offers clear operation field, high resolution, convenient application, safety and reliability. We suggest that it be adopted as a routine method in the diagnosis of mediastinal tumor and the staging of lung cancer.

Objective: To compare the clinical value of video-mediastinoscopy (VM) and standard mediastinoscopy(SM) in diagnosis of thoracic diseases. Methods: The clinical data of 100 patients with mediastinoscopy were studied retrospectively. Of them, 54 patients underwent VM, and 46 patients underwent SM. The operative parameters including operating time, complications and post-operative hospital stay, the diagnosis accuracy of undifferentiated mediastinal diseases and the accuracy of perioperative staging of lung cancer were compared. Results: The average operating time in VM group was (53.9?17.2) mins, which was less than that in SM group (69.4?24.7)mins (t=2.05,P

Objective To evaluate the role of mediastinoscopy with biopsy on right trachea-bronchial lymph node (R4) in the diagnosis and pathologic staging of lung cancer. Methods From November 1999 to May 2004, sixty-six patients with lung cancer (47 right and 19 left) received mediastinoscopic biopsy on R4 lymph node. 17 patients were preoperative diagnosed and 49 suspected have lung cancer. The approach included cervical mediastinoscopy in 62 cases, parasternal mediastinoscopy in 1 and cervical combined with parasternal mediastinoscopy in 3. Results In total 66 patients, R4 lymph node positive was found in 49 cases, including 38 patients with right lung cancer and 11 with left lung cancer. The metastasis rate in small cell lung cancer, adenocarcinoma and squamous cell carcinoma were 100%, 82.2% and 52%, respectively. For 49 preoperatively suspected lung cancer patients, malignant tumor was confirmed by mediastinoscopy with biopsy in 40 and open thoracic surgery in 9. All the operations were successful and the patients recovered smoothly without severe postoperative complications. Conclusion R4 lymph node is one of the most common and important site for the tumor metastasis in lung cancer. Mediastinoscopy with biopsy on R4 lymph node has incomparable advantages in diagnosis and staging lung cancer over other examinations and biopsy on other sites of lymph nodes.

Humans ; Lymphohistiocytosis, Hemophagocytic ; Male ; Middle Aged

Objective To study the application effect of different internal fixation scheme in older children with proximal hu-merus fractures .Methods The clinical data of 102 cases of elder children with proximal humerus fracture from March 2009 to Feb-ruary 2014 in the hospital were retrospectively analyzed according to different internal fixation methods ,they were divided randomly into A ,B ,C group ,34 cases in group A ,using elastic intramedullary retrograde intramedullary internal operation ;32 cases in group B ,using open reduction and Kirschner wire internal fixation operation ;27 cases in group C ,using open reduction and plate internal fixation operation .Compared and study the operation treatment effect on children in three groups .Results The operation time ,hos-pitalization time ,fracture healing time ,the excellent and good rate of postoperative shoulder joint Neer score ,complication rate of patients among the three groups had no statistical significance (P > 0 .05) .Compared with group B and group C ,the amount of bleeding was significantly decreased and incision length was significantly shortened in group A(P< 0 .05) .Conclusion Elastic intr-amedullary retrograte internal fixation ,kirschner wire interal fixation ,open reduction internal fixation ,all can fix proximal humeral fractures in older children safely and effectively ,and the clinical effect of elastic intramedullary retrograte internal fixation is better than other two fixation .

To investigate the inherent characteristics of the pathogenic Yersinia enterocolitica. Polymerase chain reaction (PCR),analysis of DNA sequence,randomly amplified polymorphic DNA (RAPD) were used to study the pathogenic and nonpathogenic 10 strains of Yersinia enterocolitica. The Pathogenic Yersinia enterocolitica of serotyps 0∶3?0∶9 and 0∶5,27 were confirmed containing pathogenic gene of adherent invasion locus(ail) and their DNA fingerprints were much more similar,and it was also found that the serotype 0∶22 contained ail gene whose fingerprints was partly similar with the pathogenic ones in molecular structure. The pathogenic Yersinia enterocolitica strains were similar in the inherent and polymorphic. The serotype 0∶22 may have relativly evaluated association with pathogenic Yersinia enterocolitica, which is possible that it got potentially pathogenicity.

To investigate the influential factors of different concentrations of some reagents and the comparability among the laboratories of RAPD. RAPD was carried out with different concentrations of reagents and three different amplified cyclers to amplify 11 strains of pathogenic vibrios. 11 strains of pathogenic vibvios were genotyped into 4 types, and could be easily repeated.The different concentrations of primer and 4 dNTP could influence the result of RAPD,but the fingerprints of RAPD produced respectively with 3 amplified cyclers were consistent. It is suggested that RAPD can be compared among the laboratories with the standard reagents.