AIM: To investigate the protective effects of artemisinin on mice and its inhibition effects on the release of pro-inflammatory cytokines challenged with CpG DNA. METHODS: A total of 60 mice of Kunming species were randomly divided into six groups. Animals received an intraperitoneal injection of D-galactosamine (D-Gal, 600 mg?kg -1) 1 hour prior to intravenous injection of CpG DNA. CpG DNA group received CpG DNA at 4 mg?kg -1 via caudal vein, artemisinin group were orally administered artemisinin at 200 mg?kg -1, CpG DNA plus artemisinin group first received artemisinin at 50, 100, and 200 mg?kg -1, respectively, then received CpG DNA at 4 mg?kg -1, and the control group received the saline only. The mortality was observed within seven days after injection. RAW 264.7 cell lines were cultivated in vitro and stimulated by CpG DNA to release TNF-? and IL-6, then various concentrations of artemisinin were administrated to observe its dose-dependent inhibitory effect, and artemisinin were also added at different time to observe the time-dependent effect. Contents of cytokines in culture supernatant were detected by ELISA. RESULTS: Different concentrations of artemisinin decreased the death of mice challenged with CpG DNA, and the mortality were dropped from 80% to 10% (P

Amyloid beta-Peptides ; toxicity ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Estradiol ; pharmacology ; PC12 Cells ; Peptide Fragments ; toxicity ; Rats

Objective　Genetic analysis was performed on a female child with chromosome Xq28 heterozygous deletion and suspected X-linked recessive disease to determine the morbidity and prognosis. Methods　A female child was admitted to the hospital on day 20 because of "jaundice for 20 days and difficulty in stopping bleeding at acupuncture sites". Low depth whole genome test of amniocentesis in late pregnancy suggested missing copy number of hemophilia A and X-linked mental retardation type 72. In order to further confirm the diagnosis and prognosis, peripheral blood of the children and their parents were collected for gene testing, chromosome inactivation test and genetic analysis. Results　Chromosome Xq28 of the child had 439.4 kb copy number heterozygous deletion variation, which was a clear disease-coding gene for functional loss included in ClinGen database. Chromosome inactivation test showed that the paternal X chromosome of the child was extremely inactivated. Haplotype analysis suggested that the normal chromosome of the subject was inherited from the mother, and there was heterozygous deletion on the paternal X chromosome, so it was inferred that the child will not develop disease or just have mild symptoms. Conclusion　It is necessary to analyze the X chromosome inactivation test for female patients with the pathogenic variation of X-linked recessive genetic disease to determine the possibility of the disease.

Administration, Oral ; Humans ; Rotavirus Infections ; immunology ; prevention & control ; Viral Vaccines ; administration & dosage ; immunology

Objective To explore the control measures of drug induced liver disease.Methods The age,disease drugs,clinical manifestations,treatment and prognosis of 162 cases with drug induced liver disease were analyzed retrospectively.Results Drug induced liver disease incidence in different age groups are slightly more old age group,Lead to liver damage to many types of drugs,a common anti-TB drugs and other lipid-lowering medicine and Chinese herbal medicine and health drugs can not be ignored.Clinical performance of drug-induced liver disease is different,mostly had good prognosis after treatment,a few turn into cirrhosis,a very small number of fulminant hepatic failure or even death.Conclusion Avoid the use of the drug that may injure the liver,liver protection must also drugs should be regularly reviewed liver function.

Single cell RNA sequencing (scRNA-seq) is an advanced technology to study the transcriptome information at the single cell level. The application of this technology can attribute to analyze the heterogeneous map of cells in the process of disease development, and precisely identify the specific cell subsets that are responsive to pharmacological therapy. Currently, scRNA-seq technology has been widely applied in the field of drug research, including studies on therapeutic targets, drug-induced adverse reactions, drug resistance and vaccine. This work reviews the application of scRNA-seq technology in drug discovery, which offers a scientific basis for personalized and accurate medication therapy.

Brain ; Dyspepsia ; therapy ; Gastritis ; Humans ; Medicine, Chinese Traditional ; Research Design ; Syndrome

Small and medium-sized enterprises (SMEs) are important components in Chinese medicine industry. However, the lack of big brand is becoming an urgent problem which is critical to the survival of SMEs. This article discusses the concept and traits of Chinese medicine of big brand, from clinical, scientific and market value three aspects. Guided by market value, highlighting clinical value, aiming at the scientific value improvement of big brand cultivation, we put forward the key points in cultivation, aiming at obtaining branded Chinese medicine with widely recognized efficacy, good quality control system and mechanism well explained and meanwhile which can bring innovation improvement to theory of Chinese medicine. According to the characters of SMEs, we hold a view that to build multidisciplinary research union could be considered as basic path, and then, from top-level design, skill upgrading and application three stages to probe the implementation strategy.
China ; Drug Industry ; economics ; standards ; trends ; Drugs, Chinese Herbal ; economics ; standards ; Medicine, Chinese Traditional ; economics ; standards ; trends ; Plants, Medicinal ; growth & development ; Quality Control

ObjectiveTo find out the prevalence of brucellosis in Baotou,and to provide a scientific basis for integrated control of brucellosis.MethodIn 2010,high-risk people 7 to 70 years of age engaged in livestock farming,grazing,slaughtering,processing and selling were investigated in 9 districts (banners,counties)of Baotou city.In accordance with the principle of informed consent,blood samples were collected,serum titer was tested using the rose bengal agglutination test and tube agglutination test(SAT),and serum titer of 1:100(+ +) was considered as positive brucellosis,and results were evaluated based on brucellosis monitoring standards(GB 16885-1997).ResultsA total of 5832 copy blood samples were collected,and 250 cases were tested positive,the positive rate was 4.25％.The number of new cases was 164,accounting for 2.81％ (164/5832).Age was mainly concentrated in the 40 to 60 years,accounting for 66.0％(165/250).Brucellosis infection in the livestock industry accounted for 80.87％ (4757/5882).From the regional distribution,DAMAOQI had the most detected cases,and the detection rate was 12.10％ (73/603).ConclusionsContinue to increase the brucellosis prevention knowledge propaganda,carry out brucellosis surveillance of high-risk people,quarantine,and strengthen immunity is the only measure of effective control of brucellosis epidemic.

MicroRNAs ( miRNAs) are small noncoding RNAs that post transcriptionally regulate the gene expression , involving in the process of growth , development , aging and death of organisms .It has been demonstrated that miRNAs were associated with the structure and function of blood vessels and heart , involving in the aging process of blood vessels and cardiomyocytes .For example , miR-29, miR-34a, miR-217, miR-146a may participate in the regulation of aging blood vessels;miR-21, miR-22, miR-18, miR-19 may emerge as the regulators of aging cardiomyocytes .These miRNAs may function as the novel biomarkers for the cardiovascular ag-ing.This article reviews the current studies on miRNA and cardiovascular aging .