1.Establish of animal model of nonarteritic anterior ischemic optic neuropathy
Chinese Journal of Experimental Ophthalmology 2013;(1):91-95
Nonarteritic anterior ischemic optic neuropathy (NAION)is a common optic neuropathy seriously affecting the visual function in the middle-aged and elderly population.However,its pathogenesis is not completely clear,and therefore its treating efficacy is dissatisfactory.The current study on NAION is focused on the establishment of suitable animal model and the pathogenesis.In recent years,the relatively ideal animal models(including rodent and primate) have been established by photodynamic methods,which make people have more in-depth understanding on the pathophysiologic mechanism of NAION and lay the basis for the research of therapies.The selection of experimental animals,various induction methods and existing problems in the creation of NAION animal model were reviewed and analyzed in this artical.
3.Discussion on the Ethical Quality of Medical Journal Editors
Juan WANG ; Huiqun MA ; Wen ZHENG ; Yae TANG ; Yan DONG
Chinese Medical Ethics 2016;29(4):606-608
Based on three aspects of academic research, academic morality and academic publication, this pa-per discussed the ethical quality that medical journal editors should possess. Medical knowledge and knowledge of medical ethics are the basic ethical qualities that a good medical editor should possess. Editor should strictly control the manuscript process, abide by the professional ethics, and contribute to the fast and correct spread of medical information.
5.Computer-aided Diagnosis of Gastric Carcinoma B ased on Feature Selection and Probability Neural Network
Jun LIU ; Wen-Li MA ; Wen-Juan YAO ; Wen-Ling ZHENG
Progress in Modern Biomedicine 2008;8(5):924-927
Based on signal to noise ratio and probabilistic neural network method associated with experimental data,all analysis model in gastric carcinoma is presented.According to the available information,the samples of gastric carcinoma can be tested and ana.Lyzed.The signal to noise ratio is first calculated.Secondly,records in the database are chosen as a training set to build a probabilistie neural network model and the feature subset is selected according to accuracy.Finally,test set is to test accuracy of model.The model is implemented using MATLAB,and it can be generalized and applied to similar disease auxiliary diagnosis region.
6.NB-UVB promotes melanogenesis and cell proliferation through downregulating the expression of miRNA-25 in human melanocytes
Yan DONG ; Wenbin LI ; Wen ZHENG ; Juan WANG ; Huiqun MA ; Weihui ZENG
Journal of Xi'an Jiaotong University(Medical Sciences) 2016;37(3):399-402,407
Objective To investigate the effect of narrow-band UVB (NB-UVB ) on melanocytes proliferation,melanin production,tyrosinase activities and miRNA-2 5 expression so as to explore the relationship between NB-UVB and miRNA-25 and the possible mechanism of NB-UVB for treatment of vitiligo.Methods Melanocytes cultured in vitro were treated with 40 mJ/cm2 dose of NB-UVB for 72 h,the effects of NB-UVB on cell proliferation,tyrosinase activity and melanin content were investigated.After NB-UVB stimulation for 1 2 h,the effect of NB-UVB on miRNA-2 5 expression in melanocytes was detected. Melanocytes were transfected with miRNA-2 5 mimics,miRNA-2 5 inhibitor and miRNA-2 5 mutant,respectively,the changes of cell proliferation, tyrosinase activitiy and melanin content were observed.Cell viability was detected using MTT method.Tyrosinase activities were measured with levodopa as the substrate.NaOH assay was used for the detection of melanin content. Real-time quantitative polymerase chain reaction (qRT-PCR)was used to detect miRNA-25 expression.Results After NB-UVB of 40 mJ/cm2 dose was used for the cells cultured for 72 h,the viability of melanocytes,tyrosinase activities,and melanin content were significantly increased (P<0.05 ).After NB-UVB stimulation for 12 h, miRNA-2 5 expression was significantly lower than that in the control group (P<0 .0 5 ).After knockdown of miRNA-2 5 ,the cell proliferation of melanocytes,tyrosinase activities and melanin content were increased,whereas overexpression of miRNA-2 5 decreased the cell proliferation, tyrosinase activities and melanin production.Overexpression of miRNA-2 5 partially inhibited the effect of NB-UVB on the treatment of melanocytes. Conclusion NB-UVB may promote cell proliferation,increase tyrosinase activities and melanin formation through partially downregulating the expression of miRNA-2 5 in melanocytes.
7.Effect of Acupuncture and Moxibustion plus Zushima on Serum VEGF and bF GF in Patients with Knee Osteoarthritis
Shanghai Journal of Acupuncture and Moxibustion 2019;38(2):224-228
Objective To investigate the effect of acupuncture and moxibustion plus Zushima on serum vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b FGF) in patients with knee osteoarthritis.Method Two hundred and thirty-six patients with knee osteoarthritis were allocated, using a random number table, to two groups, 118 cases each. The control group was treated with Celecoxib and the observation group, with acupuncture and moxibustion plus Zushima. The therapeutic effects, and pre-treatment and post-treatment VAS scores and WOMAC scores and serum VEGF and bFGF levels were compared between the two groups of patients. The adverse reaction incidences were compared between the two groups of patients. Result The total efficacy rate was 87.3% in the control group and 94.9% in the observation group and was significantly higher in the observation group than in the control group (P<0.05). There were no statistically significant pre-treatment differences in the VAS score and WOMAC score between the two groups (P>0.05). The VAS score and WOMAC score decreased significantly in both groups after treatment (P<0.05). After treatment, there was no statistically significant difference in the VAS score between the two groups (P>0.05) but the WOMAC score was significantly lower in the observation group than in the control group (P<0.05). There were no statistically significant pre-treatment differences in VEGF and bFGF between the two groups (P>0.05). After treatment, VEGF and bFGF decreased significantly in both groups (P<0.05) and were significantly lower in the observation group than in the control group (P<0.05). The adverse reaction incidence was3.4% in the control group and 1.7% in the observation group with no statistically significant difference between the two groups (P> 0.05). Conclusion Acupuncture and moxibustion plus Zushima can reduce inflammatory reactions, inhibit synovial angiogenesis, and effectively relive the pain, and improve articular functions in the treatment of knee osteoarthritis.
8.Research on Urine Metabolomics of HIV/AIDS Patients with Spleen-lung Qi-deifciency Based on H-NMR Technique
Qianlei XU ; Guanxiao WEN ; Juan WANG ; Shiping XIE ; Suna MA ; Ning ZHANG ; Wei LIU ; Yongxia CUI
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(2):356-361
This study was aimed to explore the characteristics of urine metabolomics among HIV/AIDS patients with spleen-lung qi-deficiency. H-NMR technique was combined with principal component analysis and cluster analysis in the comparison of urine metabolic products among 24 HIV/AIDS cases with spleen-lung qi-deficiency and 20 healthy control cases. The results showed that urine metabolic products of HIV/AIDS patients with spleen-lung qi-deficiency and healthy people by H-NMR technique detection and PLS-DA analysis can classify the outline of urine metabolites. There were about 20 variables in the difference between two groups. The speculated substances contained glycyl-L-leucine, L-valine, α-aminobutyric acid, methyl succinic acid, glycine propionyl, and etc. It was concluded that H-NMR technique was able to classify the outline of urine metabolites between HIV/AIDS cases with spleen-lung qi-deficiency and healthy people. Part of the potential existed characteristic markers contributed to the clinical diagnosis of traditional Chinese medicine (TCM) syndrome differentiation of AIDS. It had certain effect in its quantitative analysis.
10.Correlative studies on uPAR receptor mRNA expressions with vascular endothelial growth factor, microvessel density, progression and survival time of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA ; Wen-juan XU ; Zhong MENG
Chinese Journal of Pathology 2005;34(5):306-307
Adenocarcinoma
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blood supply
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metabolism
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mortality
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secondary
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Adult
;
Aged
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Female
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Humans
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Lymphatic Metastasis
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Male
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Microcirculation
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Middle Aged
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Neovascularization, Pathologic
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metabolism
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pathology
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Prognosis
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RNA, Messenger
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biosynthesis
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genetics
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Receptors, Cell Surface
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biosynthesis
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genetics
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Receptors, Urokinase Plasminogen Activator
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Stomach Neoplasms
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blood supply
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metabolism
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mortality
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pathology
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Survival Rate
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Vascular Endothelial Growth Factor A
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metabolism