Objective To compare the success rate, the one-time success rate, the average required time and the incidence of complications of two methods in oronasal conversion of nose biliary duct in naso-biliary drainage. Methods 60 cases of patients with ENBD in the ERCP in our department from March,2008 to March, 2010 were divided into the study group and the control group by random. The patients with sleeving the gastric tube by the guidewire during oronasal conversion of nose biliary duct were considered as the study group, and the patients using the traditional method were considered as the control group. The success rate of sleeving gastric tube, the one-time success rate of grasping, the average required time and the adverse reactions of the two groups were compared. Results The success rate of the study group was 100%, and the control group was 90%, there was no statistical significance. The one-time success rate of the study group was 76.7%, higher than 46.7% of the control group. The average required time of the study group were (1.3±0.3) min, shorter than (5.1±0.7) min of the control group. The incidence of complications in the study group were 13.3%, lower than 93.3% of the control group. Conclusions To sleeve the gastric tube by the guidewire can increase the one-time success rate of grasping the gastric tube, shorten time of grasping the gastric tube and significantly reduce discomfort of patients.

Objective To analyze the risk factors and distribution of pathogenic bacteria of urinary tract infection (UTI) patients with spi-nal cord injury (SCI). Methods A total of 452 patients with SCI collected from December, 2015 to June, 2016 were retrospectively analyzed. Results 104 cases (23.1%) were diagnosed as UTI. 119 strains were identified, including 96 strains of Gram-negative bacteria (80.7%) and 22 strains of Gram-positive bacteria (19.3%). Female, invasive operation, depressed lower limb muscle strength, neurogenic bladder and pre-vention of antibacterial agents use were considered to be the main risk infection factors. The invasive operation included indwelling urethral catheterization, intermittent catheterization and other transurethral operation. The incidence rate of UTI in patients with SCI who also accept-ed indwelling catheter was 100.0%. Conclusion Gram-negative bacteria is the dominated pathogenic bacteria in SCI patients with UTI. It should be noticed the characteristics of pathogen, which both may have the high drug resistance rate and high separation rate, to select exact-ly antimicrobial agents to control the spread and drug resistance of pathogenic bacteria. In addition, patients with underlying diseases, inva-sive operation and lower strength should be monitored targeted.

Objective:To understand the effect and existing problems of teaching reform about primary health care demands in local medical universities and colleges.Methods:With sampling survey method,150 students from Xi'an Medical College were investigated using questionnaires.Results:Totally 93.1％ of the freshmen approved of setting up the courses,72.6％ of the students thought that nursing ethics and skills were equally important,43.2％ of the students thought learning this course made themselves have a new understanding of nursing ethics.Totally 84.2％ of the students were satisfied with the teacher's teaching process,while 69％ of the students were not satisfied with the existing teaching materials.Conclusion:The teaching method oriented by practical problems and the teachers' own efforts are prerequisites for students' acceptance of this course.Content setting based on post needs is an important link of nursing ethics teaching effect.Setting up sustainable teaching time is the basis of the teaching effect.High quality teaching materials are necessary conditions to achieve the teaching orderly.

Anticonvulsants ; adverse effects ; Body Weight ; drug effects ; Epilepsy ; drug therapy ; Female ; Fructose ; adverse effects ; analogs & derivatives ; Humans ; Infant ; Male

Enterovirus A71 (EV-A71) causes hand, foot, and mouth disease (HFMD) and various neurological complications, including aseptic meningitis and neurogenic pulmonary edema in young children. HFMD caused by EV-A71 have broken out several times in the Asia-Pacific region since 2007. And it has been a serious threat to public health. There is no effective vaccine or antiviral drug. The pathogenesis of EV-A71 infection is unknown, and EV-A71 3C protein plays an irreplaceable role in replication and anti - innate immunity. Further research on EV-A71 3C protein is conducive to understand the pathogenesis of EV-A71 infection and antiviral drug.
Animals ; Antiviral Agents ; pharmacology ; Enterovirus ; drug effects ; immunology ; metabolism ; physiology ; Humans ; Immunity, Innate ; Viral Proteins ; chemistry ; genetics ; metabolism ; Virus Replication ; drug effects

Agenesis of Corpus Callosum ; Chromosomes, Human, X ; Gene Deletion ; Glycerol Kinase ; genetics ; Humans ; Infant ; Male ; Syndrome

Shenqi mixture tonic (C. A. mixture )is a compounded Chinese herb preparation composed of Codnopsis pilosula (Franch. )Nannf., Astragalus membranaceus Bge. and Polygonum multiflorum Thunb. Its antiaging effect was studied on 24 months old SD rats. The body weight, food intake,cardiac capacity and activities ofbrain monoamine oxidase-B(MAO-B)and red blood cell superoxide dismutase (SOD)were estimated to assess its antiaging effect. Results indicated that after feeding C. A. mixture for a fixed time the activity of MAO-B was decreased and that of red blood cell SOD increased obviously. Calorie production and cold resistant capabbility of the aged animals were improved significantly though there were no gain in body weight. In addition ,the cardiac capacity was enhanced. From the above results it can be concluded that C. A. mixture possesses evident antiaging effect

Abstract?AlM: To observe the clinical efficacy and safety of glaucoma filtration surgery combined with the application of 5 - fluorouracil for clinical treatment to offer the reference.?METHODS:A total of 91 cases of patients with glaucoma treated in our hospital were randomly divided into three groups, 30 cases of group A were given mitomycin in surgery, 31 cases of group B were given 5-fluorouracil, 30 cases of group C were given cyclosporine. They were given 12 -month-follow-up after operation, the operation success rate were recorded, the incidence of 1 -year-adverse reactions, visual acuity, intraocular pressure, low intraocular pressure, shallow anterior chamber, corneal damage index were recorded.?RESULTS: lntraocular pressure ofgroups A, B, C were respectively (20.5±8.6)mmHg,(15.2±5.5)mmHg,(20.0± 8. 1 ) mmHg, by the analysis of variance, group B was significantly lower than other two groups,P<0. 05;patients of groups A, B, C whose visual acuity increased ratios after 1y were respectively 22. 5%, 38. 1%,and 23. 1% , by the Chi. square test, the increased ratio of group B was higher than other two groups , P<0. 05; The operation success rates after 1y of operation of groups A, B, C were respectively 67. 5%,88. 1%,71. 8%,by the Chi square test, the increased ratio of group B was higher than other two groups, P<0. 05;The general incidence of adverse reaction were respectively 22. 5%,9. 5%,20. 5%,by the Chi square test , the increased ratio of group B was lower than other two groups, P<0. 05.?CONCLUSlON:Glaucoma filtration surgery combined with the application of 5-fluorouracil can improve the operation success rate, decrease the occurrence rate of adverse reaction, and safety can be improved.

Objective To study the expression of lipoprotein lipase(LPL) in human glomerular mesangial cells and the effect of very low-density lipoprotein(VLDL) on the expression of LPL.Methods LPL mRNA expression,protein synthesis and activity were detected in human glomerular mesangial cells by RT-PCR,Western blot and a radio-chemical analysis respectively.Effect of VLDL on the expression of LPL in mesangial cells was detected by Western blot.Results In human glomerular mesangial cells,a 276 bp band,that was specific for human LPL,was identified by RT-PCR,and the same of a 55 kd band,specific for human LPL by western blot.LPL activity of mesangial cells was also detected in the medium after release by heparin.VLDL stimulated LPL protein synthesis in mesangial cells in a time-and dose-dependent manner.Conclusion LPL is expressed by human mesangial cells and it has catalytic activity.Expression of LPL in mesangial cells is regulated by VLDL.